Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 282,512 +A 1.2% intergenic (+53/+170) mltB → / ← RCAP_rcc00247 membrane‑bound lytic murein transglycosylase B/NnrU family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034282,5121.→A 1.2% 4.5 ‑0.6 ‑0.4247intergenic (+53/+170)mltB/RCAP_rcc00247membrane‑bound lytic murein transglycosylase B/NnrU family protein
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (157/87):  total (160/87)
Fisher's exact test strand distribution p-value = 5.54e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.25e-01

Only 100 of 292 total aligned reads displayed.

AACGGCTGGGTGACGATCAAGCTTCTGGAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTGTTCCCGCGTGAGACAACCGG  >  NC_014034/282412‑282612
                                                                                                                                                                                                          
aaCGGCTGGGTGACGATCAAGCTTCTGGAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGAccgccg                                                                                                       >  2:2073091/1‑101 (MQ=255)
aaCGGCTGGGTGACGATCAAGCTTCTGGAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGAAATCAGCCCCGTGAccgccg                                                                                                       >  2:4316905/1‑101 (MQ=255)
 agggCTGGGTGACGATCAAGCTTCTGGAAAGCTGGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCgg                                                                                                      <  1:5543000/99‑1 (MQ=255)
      tGGGTGACGATCAAGCTTCTGGAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGcc                                                                                                 >  1:2695886/1‑101 (MQ=255)
      ggggTGACGATCAAGCTTCTGGAAACCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGcc                                                                                                 <  2:1229899/100‑1 (MQ=255)
       gggTGACGATCAAGCTTCTGGAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGccc                                                                                                <  1:496539/101‑1 (MQ=255)
       gggTGACGATCAAGCTTCTGGAAAGCCTGCGCAAAGGTTGATCTAGCCGAGGCAATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGccc                                                                                                <  1:941146/101‑1 (MQ=255)
        gttgccGATAAAGCTTCTGGAAAGCCTGCGCAAGGGTTGATCTGGCCGAGGCCATCGCGGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCa                                                                                               <  2:2195681/96‑1 (MQ=37)
         gTGACGATCAAGCTTCTGGAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCaa                                                                                              >  1:214609/1‑101 (MQ=255)
             cGATCAAGCTTCTGGAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGc                                                                                          <  2:5028965/101‑1 (MQ=255)
              gATCAAGCTTCTGGAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGcc                                                                                         <  1:1005740/101‑1 (MQ=255)
               aTCAAGCTTCTGGAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGccc                                                                                        >  1:2286989/1‑101 (MQ=255)
                tCAAGCTTCTGGAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCt                                                                                       <  1:1662704/101‑1 (MQ=255)
                tCAAGCTTCTGGAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCt                                                                                       >  2:4462978/1‑101 (MQ=255)
                tCAAGCTTCTGGAAAGCCTGCGAAAGGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCt                                                                                       <  2:4183823/101‑1 (MQ=255)
                 cAAGCTTCTGGAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTc                                                                                      <  1:3699551/101‑1 (MQ=255)
                 cAAGCTTCTGGAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTc                                                                                      >  1:1796421/1‑101 (MQ=255)
                 cAAGCTTCTGGAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTc                                                                                      >  1:3241519/1‑101 (MQ=255)
                 cAAGCTTCTGGAAAGCCGGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTc                                                                                      <  2:2281227/101‑1 (MQ=255)
                    gCTTCTGGAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGc                                                                                   >  1:4429374/1‑101 (MQ=255)
                     cTTCTGGAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGcc                                                                                  >  1:611200/1‑101 (MQ=255)
                      ttCTGGAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCa                                                                                 >  2:4131077/1‑101 (MQ=255)
                        cgttctaGCCTGCGAAACGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAgc                                                                               <  2:5198032/95‑1 (MQ=21)
                          ggAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAgcgc                                                                             >  2:4360874/1‑101 (MQ=255)
                            aaaGCCGGCGCAAAGGTTGATCGGGCCGAGGCCATCGCCGAGCCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGcgccc                                                                           <  1:422753/101‑1 (MQ=255)
                              aGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAgcagatcg                                                                         >  1:5198318/1‑95 (MQ=255)
                                  tGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGcccgccccg                                                                     >  2:5439708/1‑101 (MQ=255)
                                  tGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGcccgccccg                                                                     <  1:1431045/101‑1 (MQ=255)
                                  tGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGcccgccccc                                                                     >  2:1839948/1‑100 (MQ=255)
                                    cgcAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCccgccccgcc                                                                   >  2:754075/1‑101 (MQ=255)
                                        agggTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATg                                                               <  1:1255246/99‑1 (MQ=255)
                                        aaGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATg                                                               >  2:615261/1‑101 (MQ=255)
                                         aGGTTGATCGGGCCGGGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATgg                                                              <  1:4754079/101‑1 (MQ=255)
                                             tGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcggg                                                          >  2:2327402/1‑101 (MQ=255)
                                               aTCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcggggg                                                        >  1:4541416/1‑99 (MQ=255)
                                               aTCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcgggcg                                                        >  2:5703873/1‑101 (MQ=255)
                                               aTCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcgggcg                                                        >  2:2985806/1‑101 (MQ=255)
                                                tCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGgcg.c                                                      >  1:3893432/1‑101 (MQ=255)
                                                tCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGgcg.c                                                      >  1:2413659/1‑101 (MQ=255)
                                                 cTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcgggcgtt                                                      <  2:5748190/101‑3 (MQ=255)
                                                 cTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.Ct                                                     >  1:4277918/1‑101 (MQ=255)
                                                  tGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGggg.cct                                                    >  1:5620631/1‑99 (MQ=255)
                                                    gCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTtct                                                  <  2:280193/101‑1 (MQ=255)
                                                    gCCGAGCCCATCGCCGGGCCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTtct                                                  <  1:482771/101‑1 (MQ=255)
                                                      cGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGGG.CCtcccc                                                >  1:1148878/1‑98 (MQ=255)
                                                      cGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTtctcc                                                >  1:1881107/1‑100 (MQ=255)
                                                      cGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTtctcc                                                >  1:829862/1‑100 (MQ=255)
                                                      cGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTtcccc                                                >  2:4673026/1‑98 (MQ=255)
                                                      cGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTcg                                                >  2:1626959/1‑101 (MQ=255)
                                                      cGAGGCCACCGCCGGGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTcg                                                <  2:2695737/101‑1 (MQ=255)
                                                       gggcccacccccGGGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTcgc                                               <  1:506968/91‑1 (MQ=17)
                                                         ggCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTcgccc                                             >  1:3177856/1‑101 (MQ=255)
                                                          gCCATCGCCGGGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTcgcccg                                            <  1:2973382/101‑1 (MQ=255)
                                                          gCCATCGCCGAGCCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTcgcccg                                            <  2:3618676/101‑1 (MQ=255)
                                                            cATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTcgcccgcc                                          >  2:3179567/1‑101 (MQ=255)
                                                             aTCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCccgccg                                         <  1:4036983/101‑1 (MQ=255)
                                                             aTCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCcccccg                                         >  2:403947/1‑101 (MQ=255)
                                                             aTCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCCGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCccgccg                                         >  2:4763186/1‑101 (MQ=255)
                                                                 cgggggCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGAc                                     <  1:2590790/97‑1 (MQ=255)
                                                                 ccggcgCCCTGCGGGACATCAGCCCGGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGAc                                     <  2:3968562/96‑1 (MQ=255)
                                                                  cGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGGCCCCCGGGGCg                                    >  2:3718071/1‑101 (MQ=255)
                                                                   gAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTcgccccccccgaccg                                   >  1:1996095/1‑94 (MQ=255)
                                                                      gCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGgcg                                >  2:5221525/1‑101 (MQ=255)
                                                                      gCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCCCCGGGGCGGgcg                                >  1:3027193/1‑101 (MQ=255)
                                                                      ccccTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGgcg                                <  1:2766804/100‑1 (MQ=255)
                                                                       cccTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGgcgc                               <  2:119013/101‑1 (MQ=255)
                                                                        ccTGCGGGACATCAGCCCCGGGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGcc                              <  1:416059/101‑1 (MQ=255)
                                                                         cTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCCCCCGGGGGGGCGCCg                             >  2:5150592/1‑101 (MQ=17)
                                                                           gCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGGG.CTTCTCGCCCCCCGGGGGGGGGGCCGGc                           >  1:2120820/1‑101 (MQ=17)
                                                                           gCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCCGGACGGGGGCCGGc                           >  2:5482852/1‑101 (MQ=255)
                                                                             gggACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCct                         >  1:3452245/1‑101 (MQ=255)
                                                                             gggACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCct                         >  2:5066923/1‑101 (MQ=255)
                                                                              ggACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCctc                        >  2:3253168/1‑101 (MQ=255)
                                                                               gACACCAGCCCCGGGCCCGCGGGAGGCCCAAAGGCCCCCGGCCAGCCCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCctct                       <  1:1472315/101‑1 (MQ=14)
                                                                                aCATCAGCCCCGTGCCCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTg                      <  2:1902202/101‑1 (MQ=255)
                                                                                aCATCAGCCCCGTGACCGCCGGAGGCCAAAAGGCCCTCGGCAAGCCCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTg                      <  2:2480589/101‑1 (MQ=255)
                                                                                aCATCAGCCCCATGACCGCCGAGGGCCCAAAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCCATCGCGCCCCCCGGGGCCGgcgcgcggctct                       >  1:3162267/1‑93 (MQ=14)
                                                                                 cagcAGCCCCGGGGCGCCGGGGGGCCCAGAGCCCCTGGGCCGGCGCCCCCCCCCCCCATGCGGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTGt                     <  1:99198/98‑1 (MQ=2)
                                                                                   tCAGCCCCGGGACCCCCGGAGGCCAAAAGGCCCCCGGCCAGCCCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTGTTc                   <  1:5338604/101‑1 (MQ=14)
                                                                                     aGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGGGCCGGCCCCCGTTccc                 >  1:1985760/1‑101 (MQ=14)
                                                                                     aGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTGTTccc                 >  2:3294857/1‑101 (MQ=255)
                                                                                       cccgGTGACCGCCGGAGGCCCAAAGCCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTGTTCCcgc               <  2:1332521/101‑1 (MQ=255)
                                                                                        cccGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTGTTCCcccg              >  2:5382389/1‑101 (MQ=255)
                                                                                        cccGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCCCTGTTCCcgcg              >  1:5706656/1‑101 (MQ=255)
                                                                                        cccGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCCCTGTTCCcccg              >  1:218987/1‑101 (MQ=255)
                                                                                         ccGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTGTTCCcccgg             >  1:4645918/1‑100 (MQ=255)
                                                                                          cgggcccgccgGGGGCCCAAAGGCCCCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTGTTCCCGCGTg            <  1:1209364/96‑1 (MQ=255)
                                                                                          cgggACCGCCGGAGGCCCAAGGGCCCGCGCCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTGTTCCCGCGTg            <  1:5242796/98‑1 (MQ=17)
                                                                                          cGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTGTTCCCGCGTg            >  2:5680318/1‑101 (MQ=255)
                                                                                             gcccgccgGAGGCCCAAGGGCCCTCGGCCGGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTGTTCCCGCGTgaga         <  1:2485870/99‑1 (MQ=255)
                                                                                             gACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGGCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTGTTCCCGCGTgaga         >  2:2955730/1‑101 (MQ=255)
                                                                                             gACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.GTTCTCGCCCGCCGGGGCGGGCGGCGGCCTCTGTTTCCGCGTgaga         >  2:1519096/1‑101 (MQ=14)
                                                                                             gACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGGGCCGGCCTCTGTTTCCGCCTggga         >  2:3494029/1‑101 (MQ=14)
                                                                                              cccgccgGAGGCCCAAAGGCCCTCGGCCAGCCCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTGTTCCCGCGTGAGAc        <  2:4884500/100‑1 (MQ=255)
                                                                                                cgccgGAGGCCCAAAGGCCCTCGGCGAGCGCCCGCCCCGCCCATGGCGGGCG.GTGACACCCCGCCGGGCTGGGCGCCGGGCTCTGTTTCCGCGTGAGACaa      >  2:4594977/1‑101 (MQ=17)
                                                                                                cgccgGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTGTTCCCGCGGGAGAcca      >  2:146400/1‑99 (MQ=255)
                                                                                                 gccgGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTGTTCCCGCGTGAGACAAc     <  1:1319524/101‑1 (MQ=255)
                                                                                                 ccgggAGGCCCAAAGGCCTCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTGTTCCCGCGTGAGACAAc     <  2:1304222/98‑1 (MQ=255)
                                                                                                   cgGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCCCCCGGGCGGGCGCCCGCCCCTGTTCCCCCGTGAGACAACCg   >  2:3837272/1‑101 (MQ=17)
                                                                                                    ggAGGCCCAAGGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTGTTCCCGCGTGAGACAACCgg  <  1:3632782/101‑1 (MQ=255)
                                                                                                                                                                                                          
AACGGCTGGGTGACGATCAAGCTTCTGGAAAGCCTGCGCAAAGGTTGATCTGGCCGAGGCCATCGCCGAGGCCCTGCGGGACATCAGCCCCGTGACCGCCGGAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGACGGGCGCCGGCCTCTGTTCCCGCGTGAGACAACCGG  >  NC_014034/282412‑282612

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG