Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,880,582	+G	100%	coding (1003/1053 nt)	pdxA ←	4‑hydroxythreonine‑4‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,880,582	1	.→G	100.0%	32.3	21	coding (1003/1053 nt)	pdxA	4‑hydroxythreonine‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (20/1):  ref base (0/0):  total (20/1)

CAAGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCC.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCGGGCTGGCCTTGCCGGTGCCCGCAATGTCATAGGCCGTGCCATGATCGGGC  >  NC_014034/2880484‑2880682                                                                                                              |                                                                                                     caggggCGGAACCCCGTCAACCCCAGCCAAGGCCCGCCCCCTTCCTTTTCATCTTGCTTAAAATACCCGGGGGGTCGGGGGGCAGATCCCCCGGCG..........GCg.cg                                                                                                    <  2:4802149/98‑1 (MQ=255)  aaGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCg.cgt                                                                                                   >  2:2895368/1‑100 (MQ=255)  aaGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCg.cgt                                                                                                   >  1:2846674/1‑100 (MQ=255)  aaGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCg.cgt                                                                                                   >  1:3697065/1‑100 (MQ=255)       gggcACCCCGTCAACCGCAGCCAAACCCCGCCCCCTTCTTTTTCATTTTGCTTAAAATACCCCGGGGGCCGGGGGGCAGAGCCCCCGGCG..........GCg.cgtcacag                                                                                              <  2:2275079/100‑7 (MQ=16)       cggcAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCg.cgtcacag                                                                                              >  2:1886013/1‑95 (MQ=255)        ggcAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCCGCG..........GCg.cgtcacagc                                                                                             >  1:3541917/1‑94 (MQ=25)          cAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCg.cgtcacagccg                                                                                           >  1:1910507/1‑92 (MQ=25)            gCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGCTCCGGGGGGAGAGCCCCCGGCG..........GCG.CGCAGccgcccgg                                                                                         >  1:2168567/1‑98 (MQ=255)                                              ttttCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCcgc                                                                  >  2:3633399/1‑101 (MQ=2)                                               tttCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCcgcg                                                                 >  1:4804328/1‑101 (MQ=2)                                                 tCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGTGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGAt                                                               >  1:4320577/1‑101 (MQ=9)                                                 tCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGAt                                                               >  2:5118717/1‑101 (MQ=9)                                                 tCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGAt                                                               >  2:187688/1‑101 (MQ=9)                                                 tCATCTTGCTTCAAATACCCCGGGGGCCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGAt                                                               <  1:2629559/101‑1 (MQ=9)                                                  cATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATc                                                              >  1:798018/1‑101 (MQ=9)                                                   aTCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGGGCGGCCCAGCCGCGCCGCCCCCCTCTTCGCCGCCCTCGTCAAGGCCGCGATCa                                                             >  1:3916302/1‑101 (MQ=2)                                                   aTCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCa                                                             >  1:4823257/1‑101 (MQ=9)                                                    tCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAg                                                            >  1:1511547/1‑101 (MQ=9)                                                     cTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGa                                                           >  2:4725230/1‑101 (MQ=9)                                                      ttGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGAc                                                          >  2:3903674/1‑101 (MQ=9)                                                       tGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCCTCTTCGCCGCCATCGTCAAGGCCGCGATCAGACt                                                         >  2:4162841/1‑101 (MQ=9)                                                       tGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACt                                                         >  2:5330819/1‑101 (MQ=9)                                                         cTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGGCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACtgt                                                       >  1:4223589/1‑101 (MQ=9)                                                          ttCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCCCAGCCGCGCCGCCCCCATCTTCGCCGCCCTCGTCAAGGCCGCGATCAGACtggg                                                      >  1:5652502/1‑99 (MQ=9)                                                          ttCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACtgtg                                                      >  2:2535515/1‑101 (MQ=12)                                                          ttCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACtgtg                                                      >  2:988048/1‑101 (MQ=12)                                                           tCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGc                                                     >  1:5485522/1‑101 (MQ=12)                                                            cAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCg                                                    >  2:3718286/1‑101 (MQ=12)                                                            cAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCg                                                    >  1:5337774/1‑101 (MQ=12)                                                             aaaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCgg                                                   >  1:5394219/1‑101 (MQ=12)                                                              aaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGGGCggg                                                  >  2:1047901/1‑101 (MQ=12)                                                                                                             c.cgccgCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCGGGCTGGCCTTGCCGGTGCCCGCAATGTCATAGGCCGTGCCATGATCGGGc  <  1:476123/101‑1 (MQ=255)                                                                                                              |                                                                                                     CAAGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCC.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCGGGCTGGCCTTGCCGGTGCCCGCAATGTCATAGGCCGTGCCATGATCGGGC  >  NC_014034/2880484‑2880682 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG