Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 296,280 +T 4.8% intergenic (‑80/‑203) RCAP_rcc00258 ← / → RCAP_rcc00259 lipoprotein/porin family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034296,2800.→T 4.8% 36.2 0.0 ‑1.1267intergenic (‑80/‑203)RCAP_rcc00258/RCAP_rcc00259lipoprotein/porin family protein
Reads supporting (aligned to +/- strand):  new base (6/4):  ref base (145/109):  total (151/116)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.58e-02

Only 100 of 315 total aligned reads displayed.

CAAAGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTCCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTCCCGCCGGTCCCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTCGGTC  >  NC_014034/296180‑296382
                                                                                                                                                                                                           
cAAAGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTc                                                                                                        >  1:2326964/1‑101 (MQ=255)
 aaaGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcc                                                                                                       >  2:3483363/1‑101 (MQ=255)
 aaaGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcc                                                                                                       >  2:3397056/1‑101 (MQ=255)
 aaaGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcc                                                                                                       >  2:1919009/1‑101 (MQ=255)
 aaaGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcc                                                                                                       >  1:4017965/1‑101 (MQ=255)
   agagGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccc                                                                                                     <  1:2956564/101‑1 (MQ=255)
     agGTTGCTTTTTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccg                                                                                                   <  2:5374028/101‑2 (MQ=255)
     agGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACACGATTTTTcccccg                                                                                                   >  2:2689635/1‑100 (MQ=255)
     agGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccg                                                                                                   <  1:631661/101‑2 (MQ=255)
      ggTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccgc                                                                                                  <  2:2977068/101‑3 (MQ=255)
       gTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccgcg                                                                                                 >  2:586552/1‑98 (MQ=255)
        ttGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccgcgg                                                                                                >  2:322247/1‑97 (MQ=255)
        ttGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccgcgg                                                                                                >  1:3617534/1‑97 (MQ=255)
        ttGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccgcgg                                                                                                <  2:311305/101‑5 (MQ=255)
         tGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCggg                                                                                              <  2:703659/101‑1 (MQ=255)
          gCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGa                                                                                             <  1:3939777/101‑1 (MQ=255)
          gCTTTCTTCATACGATCGACTGCCACCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGa                                                                                             >  2:514359/1‑101 (MQ=255)
           cTTTCTTCATACGATCGACTGCCCCCGCAGGCTGACCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAc                                                                                            <  2:4526998/101‑1 (MQ=255)
           cTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAc                                                                                            <  1:590656/101‑1 (MQ=255)
           cTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAc                                                                                            >  2:1603129/1‑101 (MQ=255)
            tttCTTCATACGATCGAGTGCCCCCGCAGGCTGACCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAcc                                                                                           <  2:4887413/101‑1 (MQ=255)
            tttCTTCATACGATCGACTGCCCCCGCAGGCTGACCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAcc                                                                                           <  2:4017718/101‑1 (MQ=255)
            tttCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAcc                                                                                           >  1:384211/1‑101 (MQ=255)
             ttcttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCTGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccg                                                                                          <  2:5033472/101‑1 (MQ=255)
              tcttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgc                                                                                         >  2:2240687/1‑101 (MQ=255)
                ttcATACGATCGACTGCCCCCGCAGGCTGACCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgccc                                                                                       <  1:4128415/101‑1 (MQ=255)
                ttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgccc                                                                                       >  1:1682979/1‑101 (MQ=255)
                ttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgccc                                                                                       <  1:3763367/101‑1 (MQ=255)
                  cATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccgc                                                                                     >  1:2972363/1‑101 (MQ=255)
                   aTACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCcgcg                                                                                    >  1:4701923/1‑101 (MQ=255)
                   aTACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCcgcg                                                                                    >  1:1193548/1‑101 (MQ=255)
                    tACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCgcgg                                                                                   <  2:1193467/101‑1 (MQ=255)
                      cGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCgcggcg                                                                                 >  2:1360804/1‑101 (MQ=255)
                       gATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGcggcgg                                                                                >  1:3356638/1‑101 (MQ=255)
                          cGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGgc                                                                             >  2:524709/1‑101 (MQ=255)
                           gACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGgcg                                                                            >  1:4380621/1‑101 (MQ=255)
                           gACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGgcg                                                                            >  2:5564968/1‑101 (MQ=255)
                            aCTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGgcgc                                                                           >  1:1578172/1‑101 (MQ=255)
                                  cccGCAGGCTGACCCCTTTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTc                                                                     <  2:2188862/101‑1 (MQ=255)
                                  cccGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTc                                                                     <  1:2773903/101‑1 (MQ=255)
                                   ccGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTcc                                                                    <  1:2470742/101‑1 (MQ=255)
                                   ccGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTcc                                                                    <  1:5272715/101‑1 (MQ=255)
                                   ccGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTcc                                                                    <  1:5644862/101‑1 (MQ=255)
                                    cGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccg                                                                   >  2:4723444/1‑100 (MQ=255)
                                    cGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccc                                                                   >  2:4470035/1‑101 (MQ=255)
                                       aGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgccg                                                                <  1:2530995/101‑5 (MQ=255)
                                       aGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgccg                                                                >  2:2806411/1‑97 (MQ=255)
                                       aGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgccg                                                                <  1:78101/101‑5 (MQ=255)
                                       aGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgccg                                                                >  1:5676114/1‑97 (MQ=255)
                                       aGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgccg                                                                >  2:4165676/1‑97 (MQ=255)
                                       aGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgccg                                                                >  1:1924175/1‑97 (MQ=255)
                                       aGGCTGAACCCATTGGCTNTCCCTGTCTCTNGCCGATGGCCGNGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgccg                                                                <  1:322291/101‑5 (MQ=255)
                                        ggCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCgg                                                              >  1:93296/1‑101 (MQ=255)
                                          cTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTc                                                            >  2:1421750/1‑101 (MQ=255)
                                          cTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACC.CCCGCCCGTc                                                            >  2:5763460/1‑101 (MQ=255)
                                           tGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcc                                                           >  2:4805189/1‑101 (MQ=255)
                                             aaCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccca                                                         >  2:4252147/1‑100 (MQ=255)
                                              aCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcccag                                                        <  2:2507027/101‑3 (MQ=255)
                                               cccATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcccagc                                                       >  2:4269578/1‑98 (MQ=255)
                                               cccATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcccagc                                                       >  2:3842379/1‑98 (MQ=255)
                                                ccATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcccagcg                                                      <  2:809846/101‑5 (MQ=255)
                                                ccATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGAATTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAGCCT.CCGCCGGGcccagcg                                                      >  2:969348/1‑97 (MQ=255)
                                                 cATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCCACCT.CCGCCGGT.CCCAGCgg                                                    >  1:2376980/1‑101 (MQ=255)
                                                    tGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCggggg                                                 >  1:2680471/1‑101 (MQ=255)
                                                     ggCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGa                                                <  2:1269295/101‑1 (MQ=255)
                                                     ggCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGa                                                <  1:4607055/101‑1 (MQ=255)
                                                      gCTTTCCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGAc                                               <  1:4265257/101‑1 (MQ=255)
                                                       cTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACt                                              <  1:1746036/101‑1 (MQ=255)
                                                            cttGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGc                                         <  2:4630376/99‑1 (MQ=255)
                                                            ccTGTCTCTAGCCGATGGCCGGGGGGGCGATAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGc                                         >  2:3768315/1‑101 (MQ=255)
                                                             cTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCa                                        <  1:2846244/101‑1 (MQ=255)
                                                                 ctctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGc                                    <  1:4550235/101‑1 (MQ=255)
                                                                 ctctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGc                                    <  2:2876819/101‑1 (MQ=255)
                                                                  cgatctcGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCt                                   <  2:1402052/95‑1 (MQ=255)
                                                                   ctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCTCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTg                                  <  1:5133469/101‑1 (MQ=255)
                                                                     aGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcc                                >  1:5188459/1‑100 (MQ=255)
                                                                      gCCGATGGCCGGGGGGGCGACAAGA.TTTTCCCCCCGCGGGACCGCCCGCGGCGGGGCGCACCCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcac                               <  2:5478992/101‑1 (MQ=255)
                                                                          aTGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCAcg                           >  1:1490670/1‑101 (MQ=255)
                                                                            ggCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCAcgcg                         >  2:3561358/1‑101 (MQ=255)
                                                                               cGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGtt                      <  1:2822719/101‑1 (MQ=255)
                                                                               cGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGtt                      >  1:5784281/1‑101 (MQ=255)
                                                                               cGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGtt                      >  1:2012200/1‑101 (MQ=255)
                                                                               cGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGtt                      >  1:1006778/1‑101 (MQ=255)
                                                                                 ggggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTc                    <  1:4797615/101‑1 (MQ=255)
                                                                                  gggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGtttag                   >  1:832007/1‑99 (MQ=255)
                                                                                   ggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGc                  <  1:5481130/101‑1 (MQ=255)
                                                                                    gggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCt                 >  1:2100026/1‑101 (MQ=255)
                                                                                      gCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGGCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCttt               >  2:5687334/1‑101 (MQ=255)
                                                                                      gCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCttt               <  1:1379576/101‑1 (MQ=255)
                                                                                      gCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCttt               >  1:843292/1‑101 (MQ=255)
                                                                                      gCGACAAG.TTTTTTCCCCCGCGGGACCGCCCGCAGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCCGTTTCGCttt               <  1:5001501/101‑1 (MQ=255)
                                                                                       cGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTg              <  2:1642579/101‑1 (MQ=255)
                                                                                        gACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCCCGCGGTTTCGCTTTgg             >  2:1077629/1‑101 (MQ=255)
                                                                                           aaGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCg          >  1:3517521/1‑101 (MQ=255)
                                                                                           aaGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCg          >  2:2437767/1‑101 (MQ=255)
                                                                                           aaGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCg          >  2:2065744/1‑101 (MQ=255)
                                                                                            aagattttGCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCg          <  2:2350421/97‑1 (MQ=255)
                                                                                              gattttTCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCt        >  2:3694545/3‑101 (MQ=255)
                                                                                                ttttTCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTc      >  1:492439/1‑101 (MQ=255)
                                                                                                ttttTCCCCCGAGGGACCGCCCGTGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCCCCACGCGGTTTCGCTTTGGCCGCTTc      <  1:3421958/101‑1 (MQ=255)
                                                                                                                                                                                                           
CAAAGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTCCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTCCCGCCGGTCCCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTCGGTC  >  NC_014034/296180‑296382

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG