Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,175 +C 100% coding (1318/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1751.→C100.0% 618.0 160coding (1318/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (77/83):  ref base (0/0):  total (77/83)

Only 100 of 160 total aligned reads displayed.

GGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCAGGGCG.C.GGC.A.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCT  >  NC_014034/3256084‑3256257
                                                                                              |                                                                                            
gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                       <  2:3297064/101‑1 (MQ=255)
gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGAGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                       >  1:3713074/1‑101 (MQ=255)
 ggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cc                                                                                      <  2:1117135/101‑1 (MQ=255)
   cgcgGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccg                                                                                    <  2:4655123/101‑3 (MQ=255)
   cgcgGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccg                                                                                    <  1:4505937/101‑3 (MQ=255)
    gcggcgGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgc                                                                                   <  2:1364866/101‑4 (MQ=255)
      ggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCGTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcga                                                                                 >  2:594196/1‑96 (MQ=255)
      ggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcga                                                                                 >  1:1057283/1‑96 (MQ=255)
       gcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGAt                                                                                 <  2:4943324/101‑1 (MQ=255)
        cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccagatcg                                                                               >  2:84450/1‑94 (MQ=255)
         ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATgg                                                                               >  1:1168661/1‑101 (MQ=255)
          gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggc                                                                              >  1:1761335/1‑100 (MQ=255)
           tGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggca                                                                             <  1:3611027/101‑3 (MQ=255)
            gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGTACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcac                                                                            <  2:207738/101‑4 (MQ=255)
             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacg                                                                           <  1:1445596/101‑5 (MQ=255)
             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacg                                                                           >  1:5525771/1‑97 (MQ=255)
             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacg                                                                           >  1:5358074/1‑97 (MQ=255)
             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacg                                                                           >  1:3323786/1‑97 (MQ=255)
             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacg                                                                           >  1:4436173/1‑97 (MQ=255)
             cAGCGCGTCGCGGCCGAGGGGCGTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacg                                                                           <  1:1475947/101‑5 (MQ=255)
              aGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacgg                                                                          >  2:1520527/1‑96 (MQ=255)
                 gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctg                                                                       <  1:2067926/101‑9 (MQ=255)
                 gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctg                                                                       <  2:3897946/101‑9 (MQ=255)
                 gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctg                                                                       <  2:4210634/101‑9 (MQ=255)
                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                      <  1:2019902/101‑10 (MQ=255)
                   gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctggc                                                                     >  2:2700103/1‑91 (MQ=255)
                        ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.t                                                              >  1:2408958/1‑101 (MQ=255)
                        ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.t                                                              >  2:2486112/1‑101 (MQ=255)
                         gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.tt                                                             <  1:1595111/101‑2 (MQ=255)
                          ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttg                                                            <  2:5733711/101‑3 (MQ=255)
                          ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttg                                                            >  1:4316636/1‑99 (MQ=255)
                           cGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgc                                                           >  2:3404072/1‑98 (MQ=255)
                           cGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgc                                                           >  1:4205776/1‑98 (MQ=255)
                            gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgcc                                                          >  2:1950289/1‑97 (MQ=255)
                             aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctc                                                         >  2:5566693/1‑96 (MQ=255)
                              ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAg.ttgctcg                                                        <  1:3750444/101‑7 (MQ=255)
                                ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctcgcc                                                      >  1:1246545/1‑93 (MQ=255)
                                 gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGccc                                                      <  2:4886128/101‑1 (MQ=255)
                                  cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                     <  2:3021350/101‑1 (MQ=255)
                                  cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCg                                                     <  2:2881937/101‑1 (MQ=255)
                                   ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCgg                                                    <  1:344869/101‑1 (MQ=255)
                                   ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCgg                                                    >  2:4289346/1‑101 (MQ=255)
                                   ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCgg                                                    >  1:2804054/1‑101 (MQ=255)
                                    tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCCCCCGGc                                                   >  1:4420254/1‑101 (MQ=255)
                                     ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCa                                                  <  1:3737274/101‑1 (MQ=255)
                                     ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCa                                                  >  1:4625329/1‑101 (MQ=255)
                                     ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCa                                                  <  1:3520728/101‑1 (MQ=255)
                                       gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAgg                                                <  1:5566985/101‑1 (MQ=255)
                                        cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCCggg                                               >  1:3368931/1‑101 (MQ=255)
                                         tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAgggg                                              >  1:1214553/1‑100 (MQ=255)
                                         tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGgc                                              <  1:5415317/101‑1 (MQ=255)
                                         tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGgc                                              <  2:3227876/101‑1 (MQ=255)
                                         tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGgc                                              <  2:4022056/101‑1 (MQ=255)
                                         tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGgc                                              >  2:1198156/1‑101 (MQ=255)
                                          cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAggggg                                             >  1:230588/1‑99 (MQ=255)
                                          cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGgcg                                             >  1:3805953/1‑101 (MQ=255)
                                           aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAgggggg                                            >  2:4219636/1‑98 (MQ=255)
                                           aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCCGCACGgcgg                                            >  1:3733068/1‑100 (MQ=255)
                                           aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCCGCAAGggg.c                                           >  2:1600682/1‑101 (MQ=255)
                                              ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGG.C.ggg                                       >  1:5046813/1‑100 (MQ=255)
                                               gcggcgGGCACCGCTTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.gggc                                      <  2:2622437/101‑3 (MQ=255)
                                                 ggcggGCTCCGCAGGGCCGCGGGTCTGGCCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.gggcac                                    <  1:4579758/101‑5 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGGCCGGCAGGGGG.C.gggcac                                    >  2:2456292/1‑97 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.gggcac                                    <  2:2045056/101‑5 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.gggcac                                    <  1:2482608/101‑5 (MQ=255)
                                                  gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.ggggacg                                   >  2:1755149/1‑96 (MQ=255)
                                                  gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.gggcacg                                   <  1:5107196/101‑6 (MQ=255)
                                                   cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.c.gg                                <  2:643826/101‑1 (MQ=255)
                                                    gggCGCGGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.c.ggg                               <  1:953559/101‑2 (MQ=255)
                                                    gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCCGGGCG.CGGGC.A.c.ggg                               >  1:2651925/1‑100 (MQ=255)
                                                    gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.ggggacggg                                 >  1:470811/1‑94 (MQ=255)
                                                     ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.c.gggc                              <  1:457775/101‑3 (MQ=255)
                                                     ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.c.gggc                              <  2:78744/101‑3 (MQ=255)
                                                     ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.c.gggc                              <  2:5189755/101‑3 (MQ=255)
                                                     ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.c.gggc                              <  2:5365509/101‑3 (MQ=255)
                                                     ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCCCCCGGCAGCGCG.C.gggcacgcgc                                >  2:1881288/1‑93 (MQ=255)
                                                      gCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.c.gggcg                             >  1:3205896/1‑98 (MQ=255)
                                                        aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.c.gggcggc                           <  1:3016054/101‑6 (MQ=255)
                                                         ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.CGGGcggcc                           <  2:2553074/101‑1 (MQ=255)
                                                            cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.CGGGcggcccgc                        >  2:1737136/1‑100 (MQ=255)
                                                              tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCCC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.CGGGcggcccggcc                      <  1:16557/101‑1 (MQ=255)
                                                               ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGG.A.CGGGCGGGCCGGCCg                     >  2:4275752/1‑101 (MQ=255)
                                                                 ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCCGGGGG.CGGGC.ACC.GGCGGCCCGGCCggt                   >  1:1401926/1‑99 (MQ=255)
                                                                 ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.CGGGCGGCCCGGCCGAt                   <  1:2486254/101‑1 (MQ=255)
                                                                  cgcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCC.GGC.A.CGGGCGGGCCGGCCGATc                  >  1:1973241/1‑101 (MQ=255)
                                                                    cgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.ACC.GGCGGGCCGGCCGATCTg                >  2:1918642/1‑101 (MQ=255)
                                                                      ggTCTGACCCTGGCGGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCAGAGTTCCTCGCCCGGCAGGGCG.CGGGC.A.CGGGCGGCCCGGCCGATCTGCg              <  1:5752612/101‑1 (MQ=255)
                                                                      ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCTGAGTTGCTCGCCCGGCAGGGCG.CGGGG.A.CGGGCGGCCCGGCCGCTCTTCg              >  1:2737818/1‑101 (MQ=255)
                                                                       gTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.CGGGCGGCCCGGCCGATCTGCgg             >  2:2988739/1‑101 (MQ=255)
                                                                          tGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.C.CGGGCGGCCCCGCCCAGCTGCGTCTg          >  2:1449201/1‑101 (MQ=255)
                                                                          tGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.CGGGCGGCCCGGCCGGTCTGCGGCTg          >  2:2331576/1‑101 (MQ=255)
                                                                           gACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGCAA.G.GGCGGCCCGGCCCATCTGCgggcgg         >  2:1333428/1‑97 (MQ=255)
                                                                            aCCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.CGGGGGGCCCAGCCGATCTGCGGCTggg        >  2:3697241/1‑100 (MQ=255)
                                                                             ccctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.CGGGCGGCCCGGCCGATCTGCGGCTGGAc       <  2:352825/96‑1 (MQ=255)
                                                                             ccctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.CGGGCGGCCCGGCCGATCTGCGGCTGGAc       <  1:1950400/96‑1 (MQ=255)
                                                                             ccctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.CGGGCGGCCCGGCCGATCTGCGGCTGGAc       <  1:5333248/96‑1 (MQ=255)
                                                                              cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGGGTTGCTCGCCCGGCAGGGCG.CGGGC.A.CGGGGGGCCCGGCCGATCTGGGGCTGGGCg      >  1:1908715/5‑101 (MQ=255)
                                                                                tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.CGGGCGGCCCGGCCGATCTGCGGCTGGACggg    <  1:5254532/99‑1 (MQ=255)
                                                                                tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.CGGGCGGCCCGGCCGATCTGCGGCCGGGCggg    >  1:5165797/3‑101 (MQ=255)
                                                                                  gCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGGC.A.CGGGCGGCCCGGCCGATCTCTGGCTCGGCGGGcc  >  1:301249/1‑100 (MQ=255)
                                                                                              |                                                                                            
GGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCAGGGCG.C.GGC.A.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCT  >  NC_014034/3256084‑3256257

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG