Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,195 +G 100% coding (1338/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1951.→G100.0% 509.4 134coding (1338/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (72/62):  ref base (0/0):  total (72/62)

Only 100 of 138 total aligned reads displayed.

GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCA..GGGCG.C.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGA.TGCCGGGCGCCGCGAC  >  NC_014034/3256108‑3256278
                                                                                              |                                                                                           
ggCCGAGGGGGTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.t                                                                                     <  1:3792131/101‑1 (MQ=255)
ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.t                                                                                     >  2:1839370/1‑101 (MQ=255)
ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.t                                                                                     >  2:2486112/1‑101 (MQ=255)
ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.t                                                                                     >  1:2408958/1‑101 (MQ=255)
 gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.tt                                                                                    <  1:1595111/101‑2 (MQ=255)
  ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttg                                                                                   >  1:4316636/1‑99 (MQ=255)
  ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttg                                                                                   >  1:1334266/1‑99 (MQ=255)
  ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttg                                                                                   <  2:5733711/101‑3 (MQ=255)
   cGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgc                                                                                  >  2:3404072/1‑98 (MQ=255)
    gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgct                                                                                 >  2:2284745/1‑97 (MQ=255)
    gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgcc                                                                                 >  2:1950289/1‑97 (MQ=255)
     aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctc                                                                                >  1:3408429/1‑96 (MQ=255)
      ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAg.ttgctcg                                                                               <  1:2864701/101‑7 (MQ=255)
       gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctcgc                                                                              >  2:5587065/1‑94 (MQ=255)
         gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGccc                                                                             <  2:4886128/101‑1 (MQ=255)
         gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGccc                                                                             <  1:3580830/101‑1 (MQ=255)
          cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCg                                                                            <  2:2881937/101‑1 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCgg                                                                           >  2:4289346/1‑101 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCgg                                                                           >  1:2804054/1‑101 (MQ=255)
            tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCCCCCGGc                                                                          >  1:4420254/1‑101 (MQ=255)
             ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCa                                                                         <  1:3737274/101‑1 (MQ=255)
             ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCa                                                                         <  1:3520728/101‑1 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCA..gg                                                                     <  2:2423372/101‑1 (MQ=255)
                cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCC..ggg                                                                    >  1:3368931/1‑101 (MQ=255)
                cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..ggg                                                                    <  1:1967639/101‑1 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..gggg                                                                   >  1:1214553/1‑100 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGgc                                                                   <  1:5415317/101‑1 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGgc                                                                   >  2:1198156/1‑101 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGgc                                                                   <  2:3227876/101‑1 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGgc                                                                   <  2:4022056/101‑1 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGgcg                                                                  >  1:3805953/1‑101 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCC..GGgcgg                                                                 >  2:2989229/1‑100 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCCGCA..CGgcgg                                                                 >  1:3733068/1‑100 (MQ=255)
                      ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGGG.C.ggg                                                            >  1:5046813/1‑100 (MQ=255)
                       gcggcgGGCACCGCTTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.C.gggc                                                           <  2:2622437/101‑3 (MQ=255)
                         ggcggGCTCCGCAGGGCCGCGGGTCTGGCCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.C.gggcac                                                         <  1:4579758/101‑5 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGGCCGGCA..GGGGG.C.gggcac                                                         >  2:2456292/1‑97 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.C.gggcac                                                         <  1:2482608/101‑5 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.C.gggcac                                                         <  2:2045056/101‑5 (MQ=255)
                          gggggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.C.gggcacg                                                        <  1:1520620/99‑6 (MQ=255)
                          gcggGGACCGCGTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.C.gggcacg                                                        <  1:1198237/101‑6 (MQ=255)
                          gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.C.ggggacg                                                        >  2:1755149/1‑96 (MQ=255)
                          gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.C.gggcacg                                                        <  1:5107196/101‑6 (MQ=255)
                           cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.c.gg                                                      <  1:4457805/101‑1 (MQ=255)
                            gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCC..GGGCG.CGGGCA.c.ggg                                                     >  1:2651925/1‑100 (MQ=255)
                            gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.C.ggggacggg                                                      >  1:470811/1‑94 (MQ=255)
                             ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.c.gggc                                                    <  2:141884/101‑3 (MQ=255)
                             ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.c.gggc                                                    <  1:457775/101‑3 (MQ=255)
                             ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.c.gggc                                                    <  2:5189755/101‑3 (MQ=255)
                             ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.c.gggc                                                    <  2:78744/101‑3 (MQ=255)
                                aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.c.gggcggc                                                 <  1:3016054/101‑6 (MQ=255)
                                 ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGcggcc                                                 <  1:1403113/101‑1 (MQ=255)
                                 ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGcggcc                                                 <  2:2553074/101‑1 (MQ=255)
                                  cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGGG.CGGGCA.c.gggcgggcc                                               >  2:81590/1‑94 (MQ=255)
                                  cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGcggccc                                                <  2:2766255/101‑1 (MQ=255)
                                   gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGcggcccg                                               <  1:5407251/101‑1 (MQ=255)
                                    cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGcggcccgc                                              >  2:1737136/1‑100 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCCC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGcggcccggcc                                            <  1:16557/101‑1 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGcggcccggcc                                            <  1:2069226/101‑1 (MQ=255)
                                       ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGGA.CGGGCGGGCCGGCCg                                           >  2:4275752/1‑101 (MQ=255)
                                         ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCC..GGGGG.CGGGCACC.GGCGGCCCGGCCggt                                         >  1:1401926/1‑99 (MQ=255)
                                         ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGAt                                         <  1:2486254/101‑1 (MQ=255)
                                          cgcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCC.GGCA.CGGGCGGGCCGGCCGATc                                        >  1:1973241/1‑101 (MQ=255)
                                           gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGGCG.G.GGCC.G.GGCGGCCCGGCCCATCt                                       >  1:3035849/1‑101 (MQ=255)
                                           gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCt                                       <  2:5539830/101‑1 (MQ=255)
                                            cgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCACC.GGCGGGCCGGCCGATCTg                                      >  2:1918642/1‑101 (MQ=255)
                                            cgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTg                                      <  2:131623/101‑1 (MQ=255)
                                              ggTCTGACCCTGGCGGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCAGAGTTCCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCg                                    <  1:5752612/101‑1 (MQ=255)
                                              ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCTGAGTTGCTCGCCCGGCA..GGGCG.CGGGGA.CGGGCGGCCCGGCCGCTCTTCg                                    >  1:2737818/1‑101 (MQ=255)
                                               gTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCgg                                   >  2:2988739/1‑101 (MQ=255)
                                                tCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGGTCTGCGGc                                  >  1:4034651/1‑101 (MQ=255)
                                                tCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTggtct                                  >  1:4608120/1‑97 (MQ=255)
                                                  tGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCC.CGGGCGGCCCCGCCCAGCTGCGTCTg                                >  2:1449201/1‑101 (MQ=255)
                                                  tGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTg                                <  1:1839480/101‑1 (MQ=255)
                                                    aCCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGGGGCCCAGCCGATCTGCGGCTggg                              >  2:3697241/1‑100 (MQ=255)
                                                     ccctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGAc                             <  1:5333248/96‑1 (MQ=255)
                                                      cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGGGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGGGGCCCGGCCGATCTGGGGCTGGGCg                            >  1:1908715/5‑101 (MQ=255)
                                                       ctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCCATCTGCGGCGGGGCgg                           >  2:1494417/4‑101 (MQ=255)
                                                        tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACggg                          <  1:5254532/99‑1 (MQ=255)
                                                          gCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGGA.CGGGCGGCCCGGCCGATCTGCGGCCGGGCggggc                        >  2:3276218/1‑99 (MQ=255)
                                                             gaggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCt                     >  1:817570/11‑101 (MQ=255)
                                                             gaggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt                     <  1:4529465/91‑1 (MQ=255)
                                                                  gcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCTGA.TGc               >  1:4746278/6‑101 (MQ=255)
                                                                  gcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGA.TGc               <  2:4992620/96‑1 (MQ=255)
                                                                    agctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCCGTCTGCGGCTGGGCGGGGTCCCGA.TTCCg             >  2:2141747/4‑101 (MQ=255)
                                                                     gctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGGA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGCCCTGG.TGcccg            >  2:3698513/3‑99 (MQ=255)
                                                                     gctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGGCGGGCTGCGGCTGGAGGGGCCCCTggttcccg             >  1:4207892/3‑94 (MQ=255)
                                                                       tGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCTGA.TGCCGGgc          >  2:416208/1‑101 (MQ=255)
                                                                       tGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGA.TGCCGGgc          >  1:3739642/1‑101 (MQ=255)
                                                                       tGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGA.TGCCGGgc          <  1:5587357/101‑1 (MQ=255)
                                                                       tGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGA.TGCCGGgc          <  2:5410772/101‑1 (MQ=255)
                                                                       cggaacccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGA.TGCCGGgc          <  2:3732840/95‑1 (MQ=255)
                                                                        ggaacccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGA.TGCCGGgcg         >  2:5640913/6‑101 (MQ=255)
                                                                         gaacccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGA.TGCCGGgcgc        >  1:3338985/5‑101 (MQ=255)
                                                                         gaacccGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCACC.GGCGGGCCGGCCGCTCTGCGGCTGGGCCGGGCCCCGA.TGCGGGgcgc        >  2:2674525/5‑101 (MQ=255)
                                                                          accccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGA.TGCCGGGcgcc       <  1:5159641/98‑1 (MQ=255)
                                                                          aacccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGA.TGCCGGGcgcc       <  2:4435901/98‑1 (MQ=255)
                                                                          aacccGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATTGCCggcggc        >  1:116237/4‑97 (MQ=255)
                                                                          aacccGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGA.TGCCGGGcgcc       >  1:555364/4‑101 (MQ=255)
                                                                               gcgATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCG.CGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGA.TGCCGGGCGCCGCGAc  <  1:2456426/101‑1 (MQ=255)
                                                                                              |                                                                                           
GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCA..GGGCG.C.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGA.TGCCGGGCGCCGCGAC  >  NC_014034/3256108‑3256278

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG