BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,504,126	+G	1.9%	intergenic (‑19/+64)	recO ← / ← RCAP_rcc03309	DNA repair protein RecO/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,504,126	1	.→G	1.9%	5.2 ‑4.4 ‑0.0	253	intergenic (‑19/+64)	recO/RCAP_rcc03309	DNA repair protein RecO/hypothetical protein
Reads supporting (aligned to +/- strand): new base (4/0): ref base (80/168): total (85/168)
Fisher's exact test strand distribution p-value = 1.18e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.90e-01

Only 100 of 303 total aligned reads displayed.


TGGCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGAAGCGTGC  >  NC_014034/3504026‑3504224


tGGCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.c.c                                                                                                       >  2:2577414/1‑101 (MQ=255)


tGGCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.c.c                                                                                                       <  2:2779506/101‑1 (MQ=255)


tGGCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.c.c                                                                                                       <  1:2668157/101‑1 (MQ=255)


  gCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..gg                                                                                                   <  1:3814141/101‑1 (MQ=255)


    cGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.G.C..gggg                                                                                                 >  2:3434803/1‑101 (MQ=255)


     gTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..ggggg                                                                                                >  2:2864235/1‑101 (MQ=255)


     gTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCCCTGTCTTGCCAGTCCATGGTGCCCCGGCCGAAGG.G.C..ggggg                                                                                                >  2:1621626/1‑101 (MQ=255)


      tGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTACCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.G.C..ggcggg                                                                                               >  1:4870011/1‑100 (MQ=255)


       gAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.CGC..gggggg                                                                                               >  1:1428120/1‑100 (MQ=255)


       gAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGtt                                                                                              <  2:3828070/101‑1 (MQ=255)


        aaCACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.G.C..GGGGGgtt                                                                                             >  1:3738148/1‑101 (MQ=255)


        aaCACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.G.C..GGGGGgtt                                                                                             >  2:1388875/1‑101 (MQ=255)


        aaCACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGttt                                                                                             <  1:4880191/101‑1 (MQ=255)


        aaCACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGttt                                                                                             <  1:1766628/101‑1 (MQ=255)


         acgccTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGtttt                                                                                            <  1:2369272/98‑1 (MQ=255)


         acacCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGtttt                                                                                            <  2:5464423/101‑1 (MQ=255)


          cacCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..CGGGGTTTTg                                                                                           >  2:597879/1‑101 (MQ=255)


            ccTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCa                                                                                         <  2:4213004/101‑1 (MQ=255)


            ccTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCa                                                                                         <  2:2087664/101‑1 (MQ=255)


             cTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGGGTTGcca                                                                                        >  2:3165081/1‑99 (MQ=255)


                gACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCAcccc                                                                                     <  2:68173/101‑1 (MQ=255)


                gACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..CGGGGTGTTACAccac                                                                                     >  1:3886757/1‑99 (MQ=255)


                 aCAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCAccccc                                                                                    <  1:5054791/101‑1 (MQ=255)


                  cAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCg                                                                                   <  2:4812108/101‑1 (MQ=255)


                  cAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGGTTTTCACCCCCg                                                                                   >  2:5528413/1‑101 (MQ=255)


                      cGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGcc                                                                               <  1:3238002/101‑1 (MQ=255)


                      cGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGcc                                                                               <  2:4630075/101‑1 (MQ=255)


                      cGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGATGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGcc                                                                               <  1:4431150/101‑1 (MQ=255)


                       gCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGGTTTTCACCCCCCGGccc                                                                              >  1:5155274/1‑101 (MQ=255)


                       gCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGGTTTGCACCCCCGGGccc                                                                              >  2:1944729/1‑101 (MQ=255)


                       gCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGGTTTGCACCCCCCGGccc                                                                              >  1:5578375/1‑101 (MQ=255)


                        ccGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGcccc                                                                             <  1:2751181/101‑1 (MQ=255)


                         cGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGccccc                                                                            <  1:813215/101‑1 (MQ=255)


                         cGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGGTTTGGACCCCCGGGccccc                                                                            >  1:4808084/1‑101 (MQ=255)


                          gAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCg                                                                           <  1:159248/101‑1 (MQ=255)


                           aGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.G.C..GGGGGGTTTGCAACCCCGGGccccccc                                                                          >  2:4612363/1‑99 (MQ=255)


                           aGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGc                                                                          <  2:1451298/101‑1 (MQ=255)


                             cTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAg                                                                        <  1:4150717/101‑1 (MQ=255)


                              tttCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.G.CGGGGGGGTTTTTGCCCCCCGGCccccccgg                                                                         >  1:1981616/1‑98 (MQ=255)


                                  gCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGatat                                                                   <  2:4967104/101‑1 (MQ=255)


                                  gCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGGTTTGCACCCCCCGCCCCCCGCCGGatct                                                                   >  2:2714466/1‑99 (MQ=255)


                                   ccGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGGTTTGTACCCCCCGTACCCCCCACGATAtt                                                                  >  2:3664757/1‑101 (MQ=255)


                                    cGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATAttt                                                                 <  2:4869730/101‑1 (MQ=255)


                                     gTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTg                                                                <  1:3435008/101‑1 (MQ=255)


                                     gTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGGTTTTCACCCCCCGGCCCCCGCCGGATATTTg                                                                >  1:4353170/1‑101 (MQ=255)


                                      tGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGa                                                               <  2:3611140/101‑1 (MQ=255)


                                        cAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.CCGGGGGGTTTTCCCCCCCCGGCCCCCCCCGGGATTTTTgg                                                               >  2:2986799/1‑100 (MQ=255)


                                        cAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAgg                                                             <  1:3619501/101‑1 (MQ=255)


                                        cAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGGTTTGCACCCCCCGGCCCCCGCAGGGTATTTGAgg                                                             >  2:5184892/1‑101 (MQ=255)


                                        cAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAACG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAgg                                                             <  1:4949466/101‑1 (MQ=255)


                                           gCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCaa                                                          <  2:4321690/101‑1 (MQ=255)


                                            gcggTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCGCCCCCGGGCCCCCGCAGGATATTTGAGGCAAg                                                         <  2:5719917/99‑1 (MQ=255)


                                            cGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAg                                                         <  2:3324928/101‑1 (MQ=255)


                                             gggTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGa                                                        <  1:2936732/101‑1 (MQ=255)


                                             gggTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGa                                                        <  2:1931487/101‑1 (MQ=255)


                                              ggTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.t                                                      <  2:564289/101‑1 (MQ=255)


                                               gTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.Ta                                                     >  2:3259172/1‑101 (MQ=255)


                                                tCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.Taa                                                    <  1:2103269/101‑1 (MQ=255)


                                                 cGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.Taaa                                                   <  1:1146856/101‑1 (MQ=255)


                                                  gAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGGGGCAAGA.Taaaa                                                  >  1:1813030/1‑101 (MQ=255)


                                                  gAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGGTTTTCACCCCCCGGGCCCCCCCGGGTATTTTAGGCAAGA.Taaaa                                                  >  1:530500/1‑101 (MQ=255)


                                                    aaGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGc                                                <  2:5270659/101‑1 (MQ=255)


                                                     atgATGTTGCCTCTTTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..TGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCg                                               <  1:5758610/99‑1 (MQ=38)


                                                     aGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCg                                               <  2:155559/101‑1 (MQ=255)


                                                     aGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCg                                               <  1:164855/101‑1 (MQ=255)


                                                       gATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGaa                                             <  2:1188477/101‑1 (MQ=255)


                                                       gATCGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGATTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGaa                                             <  1:147371/101‑1 (MQ=255)


                                                         tGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAg                                           >  2:3224918/1‑101 (MQ=255)


                                                                gCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGt                                    <  2:4171967/101‑1 (MQ=255)


                                                                 cTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTc                                   >  2:1711271/1‑101 (MQ=255)


                                                                 cTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTc                                   <  1:3209848/101‑1 (MQ=255)


                                                                  tGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCa                                  <  2:5117703/101‑1 (MQ=255)


                                                                      ttGCCAGTCCATGGTGCCCGTGACGAAGGCC.C..GGGGGGTTTGCCCCCCCGGGCCCCC.CCGGGGATTTTAGGGAAGAGAAAAAGGGAAAGCGgggggggggt                               >  1:2329576/1‑95 (MQ=255)


                                                                      ttGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCt                              <  1:5664676/101‑1 (MQ=255)


                                                                       tGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCtt                             <  1:2995371/101‑1 (MQ=255)


                                                                        ggcAGTCCATGGCGCCCGTTAGGAAGG.C.C..GCGGTTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAAA.TAAAAGCGAAAGCCGGGGGCAGTCttt                            <  2:4763469/99‑1 (MQ=37)


                                                                         ccAGTCCATGGTGCCCGTGACGAAGG.C.CG.GGGGGTTTTGACCCCCCGGGCCCCCCCGGGA.ATTTTAGGGAAGA.AAAAAACGAAAACCGGGGGGAGGCtttt                           >  1:463158/1‑100 (MQ=255)


                                                                          naNNCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCa                          <  1:3459995/100‑1 (MQ=255)


                                                                          cAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCa                          >  2:4743084/1‑101 (MQ=255)


                                                                           aGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCAg                         <  2:4033135/101‑1 (MQ=255)


                                                                           aGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCAg                         >  1:1691669/1‑101 (MQ=255)


                                                                            gTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGGTTTGCACCCCCGGGGCCCCCCAGGGTATTTTAGGGAAGA.TAAAAAAGAAAACCGGGGGCAggaggtcggg                        >  1:3193156/1‑92 (MQ=255)


                                                                              ccATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTcc                      <  1:4525168/101‑1 (MQ=255)


                                                                              ccATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTcc                      <  1:767713/101‑1 (MQ=255)


                                                                                aTGGTGCCCGTGACGAAGG.C.CG.GGGGGTTTTGCCCCCCCGGGCCCCC.CCGGGTATTTTTGGGAAAG.TAAAAAAGAAAACCGGGGGCCGGCTTTTAGTCCgg                    >  2:2164194/1‑100 (MQ=255)


                                                                                  ggTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCt                  <  2:756670/101‑1 (MQ=255)


                                                                                   gTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCtt                 <  1:1257985/101‑1 (MQ=255)


                                                                                   gTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCtt                 <  1:5106704/101‑1 (MQ=255)


                                                                                    tGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTc                <  2:3737920/101‑1 (MQ=255)


                                                                                     gCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCa               >  2:2671762/1‑101 (MQ=255)


                                                                                          tGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAg          >  2:955403/1‑101 (MQ=255)


                                                                                          tGACGAAGG.C.C..GGGGGGTTTGCACCCCCGGGCCCCCGCCGGGTATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGGCAGTCTTTCAGTCCGTCTTCATCCAg          >  2:2387771/1‑101 (MQ=38)


                                                                                          tGACGAAGG.C.C..AGGGGTTTTGCACCCCCCGGCCGCCGCAGGAAATTTGAGGCCAGA.TAAAAGACAAAGACGGGGACAGGCTTTCACTCCGTCTTCACCCAg          >  2:4516111/1‑101 (MQ=255)


                                                                                           gACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGa         <  1:3353565/101‑1 (MQ=255)


                                                                                           gACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGa         <  1:4065974/101‑1 (MQ=255)


                                                                                            aCGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGaa        <  2:429098/101‑1 (MQ=255)


                                                                                            aCGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGaa        <  2:3774459/101‑1 (MQ=255)


                                                                                            aCGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGaa        <  1:5185178/101‑1 (MQ=255)


                                                                                             cGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGAAg       <  1:2777393/101‑1 (MQ=255)


                                                                                              gAAGG.C.C..GGGGGGTTTTCACCCCCCGGCCCCCCCCGGGTATTTTAGGCAAGA.TAAAAACGAAAACCGGGGGCAGTCTTTTAGTCCGTCTTCATCCAGaaac      >  2:354435/1‑99 (MQ=255)


TGGCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGG.C.C..GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGA.TAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGAAGCGTGC  >  NC_014034/3504026‑3504224

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 27 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG