Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	974,265	+C	100%	intergenic (+145/+114)	RCAP_rcc00884 → / ← RCAP_rcc00885	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	974,265	1	.→C	100.0%	112.1	31	intergenic (+145/+114)	RCAP_rcc00884/RCAP_rcc00885	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/12):  ref base (0/0):  total (19/12)

ACCCGCCGTCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCG.TGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.GGGGT..GTGCG.GAAGCCCCCGGCGGGTCGCCTCGGCGACAG  >  NC_014034/974191‑974343                                                                            |                                                                                   ggggggcttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGGGCGGCGGCGTGTTTTTTCCttt                                                           >  1:1608896/9‑100 (MQ=18)  gggggcttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGTGCAGCGGCTTGTTTTTACCTTGc                                                          >  1:3402538/8‑101 (MQ=25)  gggggcttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTTACCTTGc                                                          >  1:293582/8‑101 (MQ=37)    gggcttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGa                                                        >  1:4839415/6‑101 (MQ=255)      gcttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACa                                                      <  1:355655/98‑1 (MQ=255)        ttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTTACCTTTCGACaaa                                                    >  1:2964183/2‑101 (MQ=38)         tCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAat                                                   <  2:1534898/101‑1 (MQ=255)           gCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTTACCTTTTGACAAaaat                                                 >  1:803712/1‑101 (MQ=38)           gCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAatat                                                 <  2:2960711/101‑1 (MQ=255)            ccccccGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATc                                                <  1:2454078/101‑1 (MQ=255)            ccccccGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATc                                                <  1:4830006/101‑1 (MQ=255)            ccccccGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCAACTTGCGACAAATATc                                                >  1:3519299/1‑101 (MQ=255)              tctcGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATccc                                              <  2:2917524/98‑1 (MQ=255)               cccGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.g                                            >  1:2736461/1‑101 (MQ=255)               cccGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGAACAATATCCC.g                                            >  2:3333897/1‑101 (MQ=38)                ccGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATccccg                                            >  2:4447121/1‑99 (MQ=255)                ccGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCAGGATCCGGCATGGCCGCTGAGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.gg                                           >  1:4950761/1‑101 (MQ=38)                 cGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTTACCTTGCGACAAATATcccaga                                           >  1:2917691/1‑98 (MQ=38)                 cGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.ggg                                          <  1:42776/101‑1 (MQ=255)                  ggACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.cggg                                         >  1:3521654/1‑101 (MQ=255)                  ggACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAAAATCCC.gggg                                         >  2:4022209/1‑101 (MQ=255)                     ccccccAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTTACCTTTCGGCAAATATCCC.ggggggc                                      >  1:5112570/1‑98 (MQ=38)                     ccccccAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.gggggtc                                      >  2:1779418/1‑98 (MQ=255)                      cccccAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.gggggtcc                                     <  1:5580254/101‑5 (MQ=255)                        cccAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.gggggtccgg                                   <  2:2387074/101‑7 (MQ=39)                        cccAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.gggggtccgg                                   <  1:3857296/101‑7 (MQ=39)                        cccAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.gggggtccgg                                   <  1:1783365/101‑7 (MQ=39)                        cccAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.GGGG...GTGCg.g                               >  2:5365681/1‑101 (MQ=255)                         ccAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.gggggtccggg                                  >  1:1660081/1‑94 (MQ=38)                          cAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTTCGACAAAAATCCC.cggggtccgggg                                 >  1:1437269/1‑93 (MQ=255)                          cAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGGCAAATATCCC.gcggggccgggg                                 >  2:4624569/1‑93 (MQ=255)                           aGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.gggggtccggggg                                <  1:5698909/101‑10 (MQ=38)                                                          cgggATCCGGCATGGCCGCTGCGCAGGGGCTTGTTTTCACCTTGCGACAAATACCCCGGGGGTCGGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGCGACAg  <  1:1807502/101‑1 (MQ=9)                                                                            |                                                                                   ACCCGCCGTCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCG.TGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.GGGGT..GTGCG.GAAGCCCCCGGCGGGTCGCCTCGGCGACAG  >  NC_014034/974191‑974343 Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG