Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 978,015 +G 1.8% coding (17/522 nt) RCAP_rcc00889 ← NosL family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034978,0151.→G 1.8% 4.5 ‑3.0 ‑0.0221coding (17/522 nt)RCAP_rcc00889NosL family protein
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (101/116):  total (105/116)
Fisher's exact test strand distribution p-value = 4.94e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.63e-01

Only 100 of 266 total aligned reads displayed.

GGGTTTGGGCAGATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATGCGAGCCGGGCATCTTCAGGCCCA  >  NC_014034/977915‑978115
                                                                                                                                                                                                          
 ggTTTGGGCAGATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCCAGCGCGGTgcggcg                                                                                                      >  1:5720020/1‑101 (MQ=255)
 ggTTTGGGCAGATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTgcggcg                                                                                                      <  2:813462/101‑1 (MQ=255)
 ggTTTGGGCAGATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTgcggcg                                                                                                      >  2:535601/1‑101 (MQ=255)
 ggTTTGGGCAGATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGGgcggcg                                                                                                      >  1:1889193/1‑101 (MQ=255)
  gTTTGGGCAGATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGcggcgg                                                                                                     <  1:3172635/101‑1 (MQ=255)
   tttGGGCAGATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCggg                                                                                                    >  1:2698652/1‑101 (MQ=255)
   tttGGGCAGATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCCGCCAGCAGGCCAAGCGCGGTGCGGCggg                                                                                                    <  1:4509660/101‑1 (MQ=255)
    ttGGGCAGATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCgggg                                                                                                   >  2:1134864/1‑100 (MQ=255)
    ttGGGCAGATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGa                                                                                                   <  2:2362377/101‑1 (MQ=255)
    ttGGGCAGATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGa                                                                                                   <  2:2137966/101‑1 (MQ=255)
     tGGGCAGATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCgggga                                                                                                  >  1:1818699/1‑99 (MQ=255)
     tGGGCAGATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCgggga                                                                                                  >  2:3615549/1‑99 (MQ=255)
      gggCAGATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGaaa                                                                                                 <  1:1497075/101‑1 (MQ=255)
       ggCAGATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCTAAGCGCGGTGCGGCGGGAAAg                                                                                                <  1:3273972/101‑1 (MQ=255)
         cAGATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGCGCGGCGGGGAAggg                                                                                              >  1:51294/1‑100 (MQ=255)
          aGATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGGAAgggt                                                                                             >  2:2652424/1‑99 (MQ=255)
           gATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTTCGGCGGGGAAGGGTg                                                                                            >  1:2624488/1‑101 (MQ=255)
           gATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCCCCGAGACGCCTGCTAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTg                                                                                            <  2:4818598/101‑1 (MQ=255)
            aTCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGGAAgggggc                                                                                           >  1:4448203/1‑97 (MQ=255)
             tggAGGCGCGTCGCATCCGGGCGGGCGGCGTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGca                                                                                          <  2:1095463/99‑1 (MQ=255)
                aGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGGAAGGGTGGACAt                                                                                       >  1:431413/1‑101 (MQ=255)
                    gcgTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTc                                                                                   <  1:1328913/101‑1 (MQ=255)
                     cgTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCa                                                                                  <  1:621691/101‑1 (MQ=255)
                      gTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCCGGCCAAGCGCGGTGCGGCGGGGAAGGGTGGACATgggcca                                                                                 >  1:5314852/1‑97 (MQ=255)
                       tCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTCCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAAc                                                                                <  2:5007189/101‑1 (MQ=255)
                        cGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGGAAGGGTGGACATgggccacc                                                                               >  2:1000200/1‑95 (MQ=255)
                        cGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACt                                                                               <  2:32882/101‑1 (MQ=255)
                        cGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACt                                                                               <  1:1655799/101‑1 (MQ=255)
                         gCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGGAAGGGTGCACATGGGCAACTc                                                                              >  1:5168229/1‑101 (MQ=255)
                          cATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTcc                                                                             <  1:535651/101‑1 (MQ=255)
                           acccGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCa                                                                            <  2:3221058/99‑1 (MQ=255)
                             ccGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATc                                                                          <  2:2831203/101‑1 (MQ=255)
                             ccGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATc                                                                          <  2:3737665/101‑1 (MQ=255)
                              cgcgcgGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGGAAGGTTGCACATGGTCCACTCCATcc                                                                         >  2:774051/1‑101 (MQ=255)
                              cgcgcgGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATcc                                                                         <  1:1001090/101‑1 (MQ=255)
                               gcgcgGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATccc                                                                        <  2:2495020/101‑1 (MQ=255)
                                cgcgGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATcccc                                                                       <  1:484112/101‑1 (MQ=255)
                                 gcgggcggCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCg                                                                      <  2:4694427/101‑1 (MQ=255)
                                  cgggcggCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGGAAGGGTGCACATGGTCAACTCCATcccccg                                                                     >  1:4700629/1‑99 (MQ=255)
                                    ggcggcCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGGAAAGTTGCAAAAAGGACACTCCATACCCggac                                                                   >  2:2687738/1‑99 (MQ=255)
                                    ggcggcCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGcc                                                                   <  1:711484/101‑1 (MQ=255)
                                     gcggcCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCg                                                                  <  2:344257/101‑1 (MQ=255)
                                      cggcCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGGTGCACAAGGGCAACTCCACccccccccgg                                                                 >  1:4162453/1‑95 (MQ=255)
                                        gcCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGGGGCGGGAAAGGTTGTACATTGTCCACTCCAACCCCTGcccggc                                                               >  2:4281298/1‑97 (MQ=255)
                                         ccTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGcc                                                              <  2:4585396/101‑1 (MQ=255)
                                          cTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGGAAAGGTGCCCATGGGCAACTCCCTCCCCCGCCCTGCCg                                                             >  1:1103499/1‑101 (MQ=255)
                                          cTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCg                                                             <  2:4174849/101‑1 (MQ=255)
                                          cTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGGTGCACCTGGTCAACTCCATCCCCCGCCGGGCCg                                                             >  1:2207990/1‑101 (MQ=255)
                                              ggcaggcaAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGGTGCACATGGTCAACTCCATCCCCGGCCCTGCCggggt                                                         >  2:1626890/1‑99 (MQ=255)
                                                caggcaAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAAGGTGCCCATGGTCAAGTCCTTCCCCGGCCGTGcggcggttg                                                       >  1:2426128/1‑93 (MQ=255)
                                                 aggcaAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGGTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGGTTGc                                                      >  2:2017776/1‑101 (MQ=255)
                                                 aggcaAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGGTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGGTTGc                                                      >  2:5220365/1‑101 (MQ=255)
                                                   gcaAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAc                                                    <  1:4367246/101‑1 (MQ=255)
                                                     aaaGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCGGGGCCGGGTTTGCGC.gg                                                 >  1:3111507/1‑101 (MQ=255)
                                                       aGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGcc                                               <  1:4007771/101‑1 (MQ=255)
                                                          gCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGa                                            <  1:153400/101‑1 (MQ=255)
                                                           cAAGCGCCGAGCCGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGaa                                           <  2:5310117/101‑1 (MQ=255)
                                                               cgcCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGGTGGACATGGTAAACTCCATCCCCGGCCGGGCCGGGGTTGCAC.GGCCCGACTggt                                       >  2:1891345/1‑99 (MQ=255)
                                                                gcCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGGTGCACATGGTCACCTCCATCCCCCGCCGTGCCCGGGTTGCAC.GGCCGGGATGATg                                      >  2:396931/1‑101 (MQ=255)
                                                                   gagaCGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGGATGTTGcca                                   >  2:4865723/1‑99 (MQ=255)
                                                                    cttccgatctcagcagGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCaaa                                  <  1:1745277/91‑1 (MQ=255)
                                                                    agaCGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCaaa                                  <  2:2980307/101‑1 (MQ=255)
                                                                     gaCGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCaaaa                                 <  1:396975/101‑1 (MQ=255)
                                                                      aCGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAg                                <  2:107695/101‑1 (MQ=255)
                                                                      aCGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGGTTGCAC.GGCCGGAATGTTGCAAAAg                                >  2:4322765/1‑101 (MQ=255)
                                                                        gCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGt                              <  2:62899/101‑1 (MQ=255)
                                                                         ccTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGGTGCACATGGGCAAATCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGGATGTTGCaaaaaggc                             >  1:2723317/1‑97 (MQ=255)
                                                                          cTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGGATGTTGCAAAAGGGCa                            >  1:3686351/1‑101 (MQ=255)
                                                                           tGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGTCaa                           <  1:3615777/101‑1 (MQ=255)
                                                                           tGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGGTTGCAC.GGCCGGGATGTTGGAAAAGGGCaa                           >  1:5271137/1‑101 (MQ=255)
                                                                           tGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGGTGCACATGGTCAACTCCATCCCCGGCCGGGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAAGTCaa                           >  2:1779371/1‑101 (MQ=255)
                                                                            gCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGGTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGGTTGCAC.GGCCGGGATGTTGCAAAAGGTCaag                          >  2:2321222/1‑100 (MQ=255)
                                                                            gCCAGCAGGCCAAGCGCGGTGCCGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGTCAAt                          <  2:915671/101‑1 (MQ=255)
                                                                             ccAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATg                         <  1:888106/101‑1 (MQ=255)
                                                                             ccAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATg                         <  1:774119/101‑1 (MQ=255)
                                                                             ccAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATg                         <  1:592796/101‑1 (MQ=255)
                                                                              cagcagGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATGc                        <  2:4389102/101‑1 (MQ=255)
                                                                               agcagGCCCAGCGCAGTGCGGCGGGAAAGGTTGTACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAACGGTCAATGCg                       <  1:4999853/101‑1 (MQ=255)
                                                                               agcagGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGGTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATGCg                       >  2:1518199/1‑101 (MQ=255)
                                                                                gcagGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATGCGa                      <  2:4484922/101‑1 (MQ=255)
                                                                                 cagGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATGCGAg                     <  1:2139394/101‑1 (MQ=255)
                                                                                 cagGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATGCGAg                     <  2:17392/101‑1 (MQ=255)
                                                                                  agGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCCAAAGGTCAATGCGAGc                    >  2:1209148/1‑101 (MQ=255)
                                                                                  agGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGGTTGCCC.GGCCGGAATGTTGCAAAAGGTCAATGCGAGc                    >  1:3024256/1‑101 (MQ=255)
                                                                                  agGCCAAGCGCGGTGCGGCGGGAAAGGGTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATGCGAGc                    >  2:5628588/1‑101 (MQ=255)
                                                                                   ggCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATGCGAGcc                   >  1:2979176/1‑101 (MQ=255)
                                                                                   ggCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATGCGAGcc                   <  2:2207868/101‑1 (MQ=255)
                                                                                      cAAGCGCGGTGCGGCGGGAAAAGTTTCACCCGGTCAACTCCATCCCCGGCCCCGCCGGGGTTGGACGGGCCGGAAGGTT.CCAAAGGGCAATGCGGGGCggg                >  2:4085015/1‑101 (MQ=255)
                                                                                       aaGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGGTTGCAC.GGCCGGAATGTTGCAAAAGGGCAATGCGAGCCGGGc               >  1:490228/1‑101 (MQ=255)
                                                                                         gcgcGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGGTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATGCGAGCCGGGCAt             >  2:3994996/1‑101 (MQ=255)
                                                                                            cgGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCCGCCGTGCCGGGGTTGCAC.GGCCGGGATGTTGCAAAAGGTGAATGCGAGCCGGGCATCtt          >  1:1173227/1‑101 (MQ=255)
                                                                                            cgGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCCGCCGTGCCGGGGTTGCAC.GGCCGGAATGTTGCAAAAGGGCAATGCGAGCCGGGCATCtt          >  2:5701000/1‑101 (MQ=255)
                                                                                            cgGTGCGGCGGGAAAGGTTGCACATGGGCAACTACATCCCCGGCCGAGCCGGGTTTGCAC.GGCCGGGATGTTGCAAAAGAGCAATGCGCGcaaggaaactt          >  2:1440516/1‑91 (MQ=255)
                                                                                             ggTGCGGCGGGAAAGGGTGCACATGGTCAACTCCATCCCCCGCCGTGCCGGGGTTGCAC.GGCCGGAATGTTGCAAAAGGGCAATGCGAGCCGGGCATCTTc         >  2:3188526/1‑101 (MQ=255)
                                                                                                gcggcgGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATGCGAGCCGGGCATCTTCAgg      <  1:4567256/101‑1 (MQ=255)
                                                                                                 cggcggGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATGCGAGCCGGGCATCTTCAGGc     <  2:5137897/101‑1 (MQ=255)
                                                                                                 cggcggGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGGTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATGCGAGCCGGGCATCTTCAGGc     >  1:2264029/1‑101 (MQ=255)
                                                                                                  ggcggGAAAGGTTGAACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGCATGTTGCAAAAGGTCAATGCGAGCCGGGCATCTTCAGGcc    >  2:1330008/1‑101 (MQ=255)
                                                                                                   gcggGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATGCGAGCCGGGCATCTTCAGGccc   <  1:1209229/101‑1 (MQ=255)
                                                                                                    cggGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATTCGAGCCGGGGATCTTCAGGCCCa  >  1:3835224/1‑101 (MQ=255)
                                                                                                                                                                                                          
GGGTTTGGGCAGATCGAGGCGCGTCGCATCCGCGCGGGCGGCCTGCGGCAGGCAAAGGGCAAGCGCCGAGACGCCTGCCAGCAGGCCAAGCGCGGTGCGGCGGGAAAGGTTGCACATGGTCAACTCCATCCCCGGCCGTGCCGGGTTTGCAC.GGCCGGAATGTTGCAAAAGGTCAATGCGAGCCGGGCATCTTCAGGCCCA  >  NC_014034/977915‑978115

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 41 ≤  ATCG