Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,4030T→. 0.8% 0.0 ‑2.1 ‑0.0378intergenic (‑182/‑776)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/3):  ref base (190/185):  total (190/188)
Fisher's exact test strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.35e-01

Only 100 of 391 total aligned reads displayed.

CGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1434303‑1434503
                                                                                                       |                                                                                                    
cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCt                                                                                                      <  1:779832/101‑1 (MQ=255)
 gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTg                                                                                                     >  2:2831617/1‑101 (MQ=255)
 gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTg                                                                                                     >  2:185320/1‑101 (MQ=255)
   ggATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCg                                                                                                   >  2:5249268/1‑101 (MQ=255)
   ggATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCg                                                                                                   <  1:3030029/101‑1 (MQ=255)
     aTCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTc                                                                                                 <  1:3909779/101‑1 (MQ=255)
     aTCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTc                                                                                                 <  2:2156840/101‑1 (MQ=255)
      tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCGCTGCTTCACG.GCTGCGTCa                                                                                                <  1:2760109/101‑1 (MQ=255)
        gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAAc                                                                                              >  1:5595192/1‑101 (MQ=255)
        gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAAc                                                                                              >  1:2253286/1‑101 (MQ=255)
         aaaCCGGATGCTTGGGCCGGAACCCCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAAcc                                                                                             <  2:2727205/101‑1 (MQ=37)
         aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAAcc                                                                                             >  2:1306894/1‑101 (MQ=255)
             aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGt                                                                                         >  2:1063893/1‑101 (MQ=255)
             aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGt                                                                                         >  2:4399114/1‑101 (MQ=255)
               gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCg                                                                                       <  1:2726376/101‑1 (MQ=255)
               gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCg                                                                                       <  2:3421356/101‑1 (MQ=255)
               gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCg                                                                                       <  1:1382177/101‑1 (MQ=255)
                 tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCt                                                                                     >  1:4037421/1‑101 (MQ=255)
                  gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTc                                                                                    >  1:4178041/1‑101 (MQ=255)
                  gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTc                                                                                    <  1:2971884/101‑1 (MQ=255)
                     ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTcccc                                                                                 <  1:3298909/101‑1 (MQ=255)
                     ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTcccc                                                                                 >  1:3605684/1‑101 (MQ=255)
                      tGGGCGGAACACCTGATCCTGCTGGGTAGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTccccc                                                                                >  2:4136451/1‑101 (MQ=255)
                       gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTcccccc                                                                               >  2:1705960/1‑101 (MQ=255)
                         gCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCcgc                                                                             <  2:965104/101‑1 (MQ=255)
                          cGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCcgcg                                                                            >  1:5358397/1‑101 (MQ=255)
                           ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCgg                                                                           <  2:1151137/101‑1 (MQ=255)
                           ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCgg                                                                           >  2:1010381/1‑101 (MQ=255)
                             aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCgggg                                                                         <  2:2097317/101‑1 (MQ=255)
                             aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCgggg                                                                         >  1:5680028/1‑101 (MQ=255)
                             aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCgggg                                                                         <  2:3115403/101‑1 (MQ=255)
                              acacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCggggg                                                                        >  2:220893/1‑101 (MQ=255)
                              acacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCggggg                                                                        <  2:761706/101‑1 (MQ=255)
                               cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGc                                                                       <  2:696737/101‑1 (MQ=255)
                                 ccTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGt                                                                     <  1:4399381/101‑1 (MQ=255)
                                   tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGGTCCCCCCCCGGGGGCGTgg                                                                   >  2:4548377/1‑101 (MQ=255)
                                   tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTgg                                                                   <  1:3494887/101‑1 (MQ=255)
                                    gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGa                                                                  >  1:4346893/1‑101 (MQ=255)
                                    gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGa                                                                  >  1:5619941/1‑101 (MQ=255)
                                     gcgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTg                                                                    <  1:1832677/91‑1 (MQ=2)
                                       gggctgtcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGGGGa                                                                  >  2:4224076/9‑101 (MQ=0)
                                       gggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                  <  2:2475549/93‑1 (MQ=2)
                                       gggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGGGGa                                                                  >  1:3782163/9‑101 (MQ=0)
                                         tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                             <  1:284449/101‑1 (MQ=255)
                                           ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                           <  1:2589283/101‑1 (MQ=255)
                                            atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                             >  1:5259270/4‑101 (MQ=2)
                                            atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                                             >  1:5706284/4‑101 (MQ=2)
                                            atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCCTCGCTCCCCCCGCGGGGGGGTGGATCGaa                                                             >  1:4722987/4‑101 (MQ=2)
                                             gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                         <  2:267366/101‑1 (MQ=255)
                                              ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                                        <  1:4606331/101‑1 (MQ=255)
                                              cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                           <  2:5055095/100‑1 (MQ=2)
                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                                          <  2:5685521/101‑2 (MQ=2)
                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                                       <  2:5350320/101‑1 (MQ=255)
                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgc                                                         >  1:640131/1‑99 (MQ=2)
                                                  gccccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGTCCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctg                                                       <  1:1782793/98‑5 (MQ=2)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                    <  2:5452690/101‑1 (MQ=255)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                    >  1:95218/1‑101 (MQ=255)
                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTGCCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                                      >  1:4964940/1‑96 (MQ=2)
                                                   cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                                                   <  1:1571609/101‑1 (MQ=255)
                                                    tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                                     >  2:4479162/1‑95 (MQ=2)
                                                    tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                                     >  2:468022/1‑95 (MQ=2)
                                                    tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                                     >  1:311355/1‑95 (MQ=2)
                                                    tCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                                  <  2:3313468/101‑1 (MQ=255)
                                                     ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                                    >  1:3272680/1‑94 (MQ=2)
                                                     ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                                    >  1:4523961/1‑94 (MQ=2)
                                                      cccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctga                                                   >  2:4382530/1‑93 (MQ=2)
                                                       ccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgac                                                  <  2:5138694/101‑10 (MQ=2)
                                                       ccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                                               >  2:1933485/1‑101 (MQ=255)
                                                       ccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                                               <  2:1262437/101‑1 (MQ=255)
                                                        cccGCGGGGGCGTGGATCGAAACGGGACGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGAGGGGATCGAAACACCGagggca                                                 >  2:1457755/1‑96 (MQ=2)
                                                         ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                             >  2:198958/1‑101 (MQ=255)
                                                         ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                             <  2:1549333/101‑1 (MQ=255)
                                                          cgcgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcg                                            <  1:2202653/101‑1 (MQ=255)
                                                            cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                          <  1:1191759/101‑1 (MQ=255)
                                                            cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                          <  2:5776473/101‑1 (MQ=255)
                                                               gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                       >  1:5078828/1‑101 (MQ=255)
                                                                ggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGACGAgg                                      >  2:2512421/1‑101 (MQ=255)
                                                                 gCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAggg                                     <  2:4785836/101‑1 (MQ=255)
                                                                  cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                                    <  2:3802414/101‑1 (MQ=255)
                                                                  cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                                    >  2:2245623/1‑101 (MQ=255)
                                                                   gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                                   <  2:860979/101‑1 (MQ=255)
                                                                     ggATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                                 >  2:602994/1‑101 (MQ=255)
                                                                      gATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAgg                                >  2:4962825/1‑101 (MQ=255)
                                                                          gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                            >  2:3914543/1‑101 (MQ=255)
                                                                          gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                            <  1:4872349/101‑1 (MQ=255)
                                                                            aaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                          <  1:2440264/101‑1 (MQ=255)
                                                                             aC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCa                         >  1:1657949/1‑101 (MQ=255)
                                                                                 gTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                       >  1:3998219/1‑101 (MQ=255)
                                                                                  tctcCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc                      >  2:2348026/1‑101 (MQ=255)
                                                                                   ctcCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg                     >  1:3213500/1‑101 (MQ=255)
                                                                                   ctcCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg                     <  2:2783174/101‑1 (MQ=255)
                                                                                    tcCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGc                    <  2:2921043/101‑1 (MQ=255)
                                                                                      cGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTc                  <  1:2010432/101‑1 (MQ=255)
                                                                                       gCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc                 <  2:162664/101‑1 (MQ=255)
                                                                                           tGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc             <  1:1031937/101‑1 (MQ=255)
                                                                                            gCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTAGCTCCCCCcg            <  1:1455275/101‑1 (MQ=255)
                                                                                            cttccgatctcTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc             <  1:3318219/91‑1 (MQ=255)
                                                                                                 aCG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCaggg       >  2:3861307/1‑101 (MQ=255)
                                                                                                      cTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGt   >  1:2373406/1‑101 (MQ=39)
                                                                                                       tGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCggg  >  1:4486504/1‑99 (MQ=38)
                                                                                                       |                                                                                                    
CGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1434303‑1434503

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG