Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,434,539	1	.→A	100.0%	2.5	6	intergenic (‑318/‑640)	RCAP_rcc01334/RCAP_rcc01335	hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (2/3):  ref base (0/1):  total (2/4)

TTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCCGCGGCG.TTTGACCTGGTCGCGG..GCAGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1434475‑1434577                                                                   |                                        tcgatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCT.GGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATc      <  2:911031/95‑1 (MQ=2) gtgatcgTCGCTCCCCCCGCGGGGGCGTGGATCAAAACCTATGAGGGCT.GGCGGCCGGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATc      <  2:3831383/95‑1 (MQ=2)   ggtggTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCCCCGGCGCCGCAGCGAGTGCGTCTGGA..ACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa   <  2:1437358/97‑1 (MQ=2)   gatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCT.GGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa    >  2:3990375/5‑101 (MQ=2)    atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCT.GGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa   <  2:4620679/98‑1 (MQ=2)     tcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCT.GGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa  >  1:5015058/3‑101 (MQ=2)                                                                   |                                        TTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCCGCGGCG.TTTGACCTGGTCGCGG..GCAGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1434475‑1434577 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG