Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,086,542 G→T 100% P584T (CCG→ACG)  RCAP_rcc01002 ← integrase catalytic subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,086,5420G→T100.0% 1202.3 310P584T (CCG→ACG) RCAP_rcc01002integrase catalytic subunit
Reads supporting (aligned to +/- strand):  new base (160/150):  ref base (0/0):  total (160/150)

Only 100 of 320 total aligned reads displayed.

AACGCAGATCAGTTTGCCGGGCTGCCCGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGGCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGATCTCGTAGGGGCGGAAAAGA  >  NC_014034/1086444‑1086639
                                                                                                  |                                                                                                 
   gCAGATCAGTTTGCCGGGCTGCCCGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGt                                                                                              <  2:3305272/101‑1 (MQ=255)
    cAGATCAGTTTGCCGGGCTGCCCGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTg                                                                                             <  1:561074/101‑1 (MQ=255)
    cAGATCAGTTTGCCGGGCTGCCCGGTTTCCTTGGCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTg                                                                                             <  1:4958601/101‑1 (MQ=255)
     aGATCAGTTTGCCGGGCTGCCCGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGa                                                                                            >  1:1461977/1‑101 (MQ=255)
     aGATCAGTTTGCCGGGCTGCCCGGTTTCCTTGTCGAACTCGCGCACCCAGAACCGATCGGCCTGGTGATAGACGTAACCGACCATCACATTCGTCTCGTGa                                                                                            >  2:913679/1‑101 (MQ=255)
           gTTTGCCGGGCTGCCCGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCTTGCTAgggg                                                                                      >  2:1991439/1‑101 (MQ=255)
           gTTTGCCGGGCTGCCCGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAgggg                                                                                      <  2:5451080/101‑1 (MQ=255)
            tttGCCGGGCTGCCCGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAggggg                                                                                     >  1:2155690/1‑101 (MQ=255)
            tttGCCGGGCTGCCCGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCATCGTCTCGTGATAggggg                                                                                     <  1:169671/101‑1 (MQ=255)
             ttGCCGGGCTGCCCGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGc                                                                                    <  2:3972518/101‑1 (MQ=255)
                   ggCTGCCCGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAgcgc                                                                              >  1:688513/1‑101 (MQ=255)
                     cTGCCCGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGcgcgg                                                                            >  1:2911037/1‑100 (MQ=255)
                     cTGCCCGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGt                                                                            >  2:1442225/1‑101 (MQ=255)
                      tGCCCGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTg                                                                           >  1:3037236/1‑101 (MQ=255)
                       gCCCGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGa                                                                          <  2:3152094/101‑1 (MQ=255)
                       gCCCGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGa                                                                          >  2:1598408/1‑101 (MQ=255)
                       gCCCGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGa                                                                          <  1:4122913/101‑1 (MQ=255)
                        cccGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGAt                                                                         <  1:5246227/101‑1 (MQ=255)
                        cccGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGAt                                                                         <  2:4375345/101‑1 (MQ=255)
                         ccGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATc                                                                        >  1:2008621/1‑101 (MQ=255)
                         ccGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGGGATc                                                                        >  2:1007808/1‑101 (MQ=255)
                          cGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCg                                                                       >  1:343074/1‑101 (MQ=255)
                          cGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCg                                                                       <  1:5377102/101‑1 (MQ=255)
                          cGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCg                                                                       <  2:1156298/101‑1 (MQ=255)
                           ggTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGa                                                                      >  1:5696784/1‑101 (MQ=255)
                           ggTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGa                                                                      <  1:2289483/101‑1 (MQ=255)
                            atttCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGaa                                                                     >  2:5422072/2‑101 (MQ=255)
                             tttCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGaaa                                                                    >  1:1184940/1‑101 (MQ=255)
                              ttCCTTGTCGAACTCGCGCAGCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCTCCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAAt                                                                   <  2:5749914/101‑1 (MQ=255)
                              ttCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAAt                                                                   >  1:3019668/1‑101 (MQ=255)
                                ccTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATAc                                                                 <  1:5495978/101‑1 (MQ=255)
                                 cTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACt                                                                >  1:5030947/1‑101 (MQ=255)
                                  ttGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACtg                                                               >  2:3786208/1‑101 (MQ=255)
                                  ttGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACtg                                                               <  2:2725807/101‑1 (MQ=255)
                                  ttGTCGAACTCGCGCACCCAGACCCGATCAGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACtg                                                               <  2:182978/101‑1 (MQ=255)
                                   tGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACtgg                                                              <  2:3864732/101‑1 (MQ=255)
                                      cGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACtggttg                                                           <  2:4082931/101‑1 (MQ=255)
                                      cGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACtggttg                                                           >  1:1930278/1‑101 (MQ=255)
                                      cGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACtggttg                                                           <  2:688456/101‑1 (MQ=255)
                                      cGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACtggttg                                                           <  2:4649647/101‑1 (MQ=255)
                                       nanNNCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACtggttgg                                                          <  1:4198464/100‑1 (MQ=255)
                                       gAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACtggttgg                                                          >  2:5138703/1‑101 (MQ=255)
                                        aaCTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACtggttggt                                                         <  2:2912184/101‑1 (MQ=255)
                                          cTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGgtgt                                                       >  2:2138278/1‑101 (MQ=255)
                                          cTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGgtgt                                                       >  1:865636/1‑101 (MQ=255)
                                            cgcgCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTc                                                     >  2:4228349/1‑101 (MQ=255)
                                                cACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCcaca                                                 <  1:362535/101‑1 (MQ=255)
                                                cACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCcaca                                                 <  2:3029287/101‑1 (MQ=255)
                                                 ccccAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAg                                                <  2:3706285/100‑1 (MQ=255)
                                                 aCCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAg                                                >  1:1674863/1‑101 (MQ=255)
                                                   ccAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGGTAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGGGTTCCACAGCa                                              >  1:4920077/1‑101 (MQ=255)
                                                   ccAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCa                                              >  2:5321930/1‑101 (MQ=255)
                                                    cAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCAc                                             <  2:3631783/101‑1 (MQ=255)
                                                    cAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCAc                                             >  1:4297495/1‑101 (MQ=255)
                                                    cAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCAc                                             <  1:3582883/101‑1 (MQ=255)
                                                     aGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCAcc                                            >  1:1819506/1‑101 (MQ=255)
                                                     aGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCAcc                                            <  1:3716299/101‑1 (MQ=255)
                                                     aGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCAcc                                            >  1:1509325/1‑101 (MQ=255)
                                                      gACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCt                                           <  1:1382516/101‑1 (MQ=255)
                                                      gACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGAACCt                                           >  2:1111611/1‑101 (MQ=255)
                                                       aCCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTc                                          >  1:391051/1‑101 (MQ=255)
                                                          cGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAgatcggaaga                                       >  2:1323958/1‑92 (MQ=255)
                                                           gATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTcgccg                                      >  2:20593/1‑101 (MQ=255)
                                                              cGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTcgccgcgc                                   >  2:2269857/1‑101 (MQ=255)
                                                              cGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTcgccgcgc                                   >  1:3383924/1‑101 (MQ=255)
                                                              cGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGAACCTcgccgcgc                                   >  2:1864084/1‑101 (MQ=255)
                                                               ggCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTcgccgcgcc                                  <  2:349884/101‑1 (MQ=255)
                                                                gCCTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGAGAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTcgccgcgccg                                 >  2:2959438/1‑101 (MQ=255)
                                                                  cTGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGACGCGCCgtg                               >  2:4277733/1‑101 (MQ=255)
                                                                   tGGTGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCgtgt                              <  1:340123/101‑1 (MQ=255)
                                                                      tGATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGt                           >  1:4745801/1‑101 (MQ=255)
                                                                       gATAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTa                          <  1:585344/101‑1 (MQ=255)
                                                                        aTAGTCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGtcc                         >  2:4977634/1‑99 (MQ=255)
                                                                          aGTCGTAACCGACCATCACCTTCGTCTCGGGATAGGGGGAAAGCGCGTGAGCGAAATACTGGTTGGTGTTCCAAAGCACCTCGCCGCGCCGTGTGGAACgg                       >  2:1521187/1‑101 (MQ=255)
                                                                           gTCGTAACCGACCATCGCCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTGCCACAGCACCTCGCCGCGCCGTGTGGTACGGa                      <  2:806283/101‑1 (MQ=255)
                                                                            tCGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGGGGTACGGAt                     >  2:2521826/1‑101 (MQ=255)
                                                                             cGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGAtc                    >  1:765711/1‑101 (MQ=255)
                                                                             cGTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGAtc                    >  1:243693/1‑101 (MQ=255)
                                                                              gTAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGAtct                   >  2:1675964/1‑101 (MQ=255)
                                                                               tAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGAtctc                  >  2:4739865/1‑101 (MQ=255)
                                                                               tAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGAtctc                  <  1:4411272/101‑1 (MQ=255)
                                                                               tAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGAtctc                  >  1:2739721/1‑101 (MQ=255)
                                                                               tAACCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGAtctc                  <  2:5787304/101‑1 (MQ=255)
                                                                                aaCCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGATCTCg                 >  1:3460406/1‑101 (MQ=255)
                                                                                aaCCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGATCTCg                 <  1:4557138/101‑1 (MQ=255)
                                                                                aaCCGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGATCTCg                 >  2:3393780/1‑101 (MQ=255)
                                                                                  ccGACCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGATCTCGTa               >  2:5602629/1‑101 (MQ=255)
                                                                                     aCCATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGATCTCGTAggg            <  2:2626285/101‑1 (MQ=255)
                                                                                      ccATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGATCTCGTAgggg           >  1:404100/1‑101 (MQ=255)
                                                                                      ccATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGATCTCGTAgggg           <  1:1080896/101‑1 (MQ=255)
                                                                                       cATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCTCCGCGCCGTGTGGTACGGATCTCGTAGGGGc          <  1:5138989/101‑1 (MQ=255)
                                                                                       cATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGATCTCGTAGGGGc          >  2:5026280/1‑101 (MQ=255)
                                                                                       cATCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGATCTCGTAGGGGc          <  1:908280/101‑1 (MQ=255)
                                                                                        aTCACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGATCTCGTAGGGGCg         >  1:2811063/1‑101 (MQ=255)
                                                                                          cACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGATCTCGTAGGGGCGGa       >  2:4554147/1‑101 (MQ=255)
                                                                                          cACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGATCTCGTAGGGGCGGa       >  2:2425231/1‑101 (MQ=255)
                                                                                          cACCTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGATCTCGTAGGGGCGGa       >  2:2110424/1‑101 (MQ=255)
                                                                                            ccTTCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGATCTCGTAGGGGCGGaaa     <  1:3226749/101‑1 (MQ=255)
                                                                                              ttCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGATCTCGTAGGGGCGGAAAAg   <  1:2651937/101‑1 (MQ=255)
                                                                                               tCGTCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGATCTCGTAGGGGCGGAAAAGa  >  1:2546509/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
AACGCAGATCAGTTTGCCGGGCTGCCCGGTTTCCTTGTCGAACTCGCGCACCCAGACCCGATCGGCCTGGTGATAGTCGTAACCGACCATCACCTTCGGCTCGTGATAGGGGGCAAGCGCGTGATCGAAATACTGGTTGGTGTTCCACAGCACCTCGCCGCGCCGTGTGGTACGGATCTCGTAGGGGCGGAAAAGA  >  NC_014034/1086444‑1086639

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG