BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,479	C→T	16.0%	intergenic (+291/‑640)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,479	0	C→T	16.0%	155.1 ‑1.4 ‑0.4	355	intergenic (+291/‑640)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (33/24): ref base (146/150): total (179/176)
Fisher's exact test strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.98e-01

Only 100 of 375 total aligned reads displayed.


GGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCG  >  NC_014034/1395379‑1395579


gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                                                                                       >  1:5033205/1‑101 (MQ=255)


ggggaCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                                                                                       >  1:5235853/1‑101 (MQ=255)


  gggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCgg                                                                                                     >  1:4556070/1‑101 (MQ=255)


  gggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCgg                                                                                                     >  2:3384338/1‑101 (MQ=255)


    gCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCCCTTTGGCGGTc                                                                                                   >  1:1277530/1‑101 (MQ=255)


     cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCg                                                                                                  <  1:3938075/101‑1 (MQ=255)


     cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCg                                                                                                  <  1:4127395/101‑1 (MQ=255)


     cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCg                                                                                                  >  2:2737899/1‑101 (MQ=255)


      gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCTCGCGGGGGCGTGGATCGAAACATCGCCGC.TTGGCGGTCCAc                                                                                                >  1:2305269/1‑101 (MQ=255)


        ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTCGCGGTCGACa                                                                                               >  2:3030113/1‑101 (MQ=255)


         gATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                                                                              <  2:5335712/101‑1 (MQ=255)


         gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                                                                              >  2:2130315/1‑101 (MQ=255)


          aTCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGGGGTCGCTCCCCCCGCGGGGACGTTGATCGAAACATCGCCGCTTTGGCGGTCGGCAgg                                                                                             >  1:4949447/1‑101 (MQ=38)


            cGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                                                                                           <  2:3248864/101‑1 (MQ=255)


             gAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTg                                                                                          <  2:2249688/101‑1 (MQ=255)


              aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                                                                         >  2:5587427/1‑101 (MQ=255)


              aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGCGGACAGGCTgg                                                                                         >  1:2584412/1‑101 (MQ=255)


               aaCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGt                                                                                        >  2:570184/1‑101 (MQ=255)


                 cTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGGaa                                                                                      >  2:4002762/1‑101 (MQ=255)


                   gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                                                                                    >  1:4772535/1‑101 (MQ=255)


                    cACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgt                                                                                   <  1:1008518/101‑1 (MQ=255)


                    cACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgt                                                                                   >  1:2010865/1‑101 (MQ=255)


                      cccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgtgt                                                                                 <  2:2638602/101‑1 (MQ=255)


                       ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTc                                                                                <  2:434386/101‑1 (MQ=255)


                       ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTc                                                                                >  2:2208261/1‑101 (MQ=255)


                          cTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCt                                                                             <  1:2864074/101‑1 (MQ=255)


                          cTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCt                                                                             <  2:289402/101‑1 (MQ=255)


                          cTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGGAAAGTGTCGCt                                                                             >  2:1030972/1‑101 (MQ=255)


                           tGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGcgc                                                                            >  1:2806509/1‑99 (MQ=255)


                           tGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                                                                            <  1:3312939/101‑1 (MQ=255)


                            ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                                                                           <  2:5438441/101‑1 (MQ=255)


                              cccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccc                                                                         <  1:3139766/101‑1 (MQ=255)


                                cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                                       >  1:2420560/1‑101 (MQ=255)


                                cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                                       >  1:3545085/1‑101 (MQ=255)


                                cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                                       <  1:4641189/101‑1 (MQ=255)


                                    cggtgatcggtgatCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgg                                                                   >  2:3436785/1‑101 (MQ=255)


                                       tgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggggg                                                                >  2:1290923/1‑101 (MQ=255)


                                       tgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggggg                                                                >  1:5062271/1‑101 (MQ=255)


                                       tgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggggg                                                                >  1:1772931/1‑101 (MQ=255)


                                        gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                               <  2:794523/101‑1 (MQ=255)


                                        gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                               <  2:1433974/101‑1 (MQ=255)


                                            ggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGGgg                                                           >  2:4656103/1‑101 (MQ=255)


                                             gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa                                                          <  1:3809293/101‑1 (MQ=255)


                                             gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGCGGCGTGGa                                                          <  2:166269/101‑1 (MQ=255)


                                             gctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  2:3797524/92‑1 (MQ=2)


                                              tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         >  1:5728610/1‑101 (MQ=255)


                                              tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  1:867993/101‑1 (MQ=255)


                                              ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:2868548/93‑1 (MQ=0)


                                               tggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       >  2:5129891/8‑101 (MQ=2)


                                               gatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:627700/101‑1 (MQ=255)


                                                atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGCGTGGGTCCCGCCGCggggggcgggcgcg                                                       >  2:2599967/1‑92 (MQ=25)


                                                atcAGCGCTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTCGCCGCTTCGGCGGACGACAGGCTGTTAACGTGTCGCTCCCCCCCCCGGGGCGAggctta                                                       >  2:3901125/1‑97 (MQ=25)


                                                 tcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      <  1:3748413/101‑1 (MQ=255)


                                                    gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                  >  2:724624/3‑101 (MQ=2)


                                                     tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACag                                                 >  1:4020515/2‑100 (MQ=2)


                                                     gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                  <  1:1646531/100‑1 (MQ=255)


                                                      ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACagc                                                <  1:592333/101‑3 (MQ=2)


                                                      ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACagc                                                >  2:2668049/1‑99 (MQ=2)


                                                      ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                 >  1:5437641/1‑101 (MQ=255)


                                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtc                                                <  2:3949811/101‑1 (MQ=255)


                                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtc                                                >  2:1938972/1‑101 (MQ=255)


                                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctt                                              >  2:4804208/1‑100 (MQ=2)


                                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtct                                               >  2:1560552/1‑101 (MQ=255)


                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                              >  1:3034850/1‑101 (MQ=255)


                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                              >  1:5623428/1‑101 (MQ=255)


                                                         cGCTCCCCCAGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgcttt                                             >  2:4720972/1‑99 (MQ=2)


                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttc                                            <  1:1508051/101‑4 (MQ=2)


                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttc                                            <  1:2954674/101‑4 (MQ=2)


                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                             <  2:5235566/101‑1 (MQ=255)


                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                           <  1:3475614/101‑5 (MQ=2)


                                                            tCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaa                                          >  2:2439313/1‑96 (MQ=2)


                                                                cccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCC.GTGCCg                                      >  1:956244/1‑101 (MQ=255)


                                                                  cgcgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTc                                    <  2:2023410/101‑1 (MQ=255)


                                                                  cgcgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTc                                    <  2:1330235/101‑1 (MQ=255)


                                                                   gcgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCACCGCGGGGGCGTGGATCGAAACATCTCG.GTGCGGTCg                                   >  1:5560049/1‑101 (MQ=255)


                                                                    cgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGc                                  >  1:2720527/1‑101 (MQ=255)


                                                                     gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCggt                                 >  2:3572195/1‑99 (MQ=255)


                                                                     gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCt                                 >  1:1151582/1‑101 (MQ=255)


                                                                       gggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCg                               <  2:1746213/101‑1 (MQ=255)


                                                                       gggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCg                               >  2:4109415/1‑101 (MQ=255)


                                                                          cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGTATGCCGTcttctgct                             >  1:5293820/1‑94 (MQ=37)


                                                                          cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTcc                            <  2:5546908/101‑1 (MQ=255)


                                                                            tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAg                          <  1:710469/101‑1 (MQ=255)


                                                                              aaTCCAACCATCGCCGGTTTGGCGGTCGACAGGCTGGTAACGGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGcc                        <  2:4542997/100‑1 (MQ=37)


                                                                               aTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCg                       <  1:3367757/101‑1 (MQ=255)


                                                                                tCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCgg                      >  1:440305/1‑101 (MQ=255)


                                                                                tCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCgg                      <  2:5032922/101‑1 (MQ=255)


                                                                                 cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGc                     >  2:4165013/1‑101 (MQ=255)


                                                                                    aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTg                  <  1:1058147/101‑1 (MQ=255)


                                                                                    aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTg                  <  1:1315308/101‑1 (MQ=255)


                                                                                    aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTg                  >  1:321111/1‑101 (MQ=255)


                                                                                     aCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCCGCCGGCCTGc                 >  1:3661510/1‑101 (MQ=255)


                                                                                      cATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCt                >  1:1446338/1‑101 (MQ=255)


                                                                                      cATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCt                <  2:2303306/101‑1 (MQ=255)


                                                                                       aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGccc               >  2:2955141/1‑99 (MQ=255)


                                                                                        tCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCtcg              <  2:815338/101‑1 (MQ=255)


                                                                                         cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCtcgt             <  1:3501539/101‑1 (MQ=255)


                                                                                               tttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcc       <  1:2138662/101‑1 (MQ=255)


                                                                                                ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTccc      >  2:4851943/1‑101 (MQ=255)


                                                                                                 tGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcccc     >  1:4325602/1‑101 (MQ=255)


GGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCG  >  NC_014034/1395379‑1395579

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG