Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,499 T→A 16.8% intergenic (+311/‑620) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4990T→A16.8% 164.3 ‑2.5 ‑0.5340intergenic (+311/‑620)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (33/24):  ref base (126/156):  total (160/180)
Fisher's exact test strand distribution p-value = 8.09e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.27e-01

Only 100 of 375 total aligned reads displayed.

CACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACC  >  NC_014034/1395399‑1395599
                                                                                                    |                                                                                                     
cACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgt                                                                                                       <  1:1008518/101‑1 (MQ=255)
  cccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgtgc                                                                                                     <  2:5292777/101‑2 (MQ=255)
   ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTc                                                                                                    <  2:434386/101‑1 (MQ=255)
      cTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCt                                                                                                 >  2:3858337/1‑101 (MQ=255)
       tGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                                                                                                <  1:3312939/101‑1 (MQ=255)
       tGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                                                                                                >  2:5218121/1‑101 (MQ=255)
           ccTGCCGGTGATTGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccccc                                                                                            >  1:3138296/1‑101 (MQ=255)
           ccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccccc                                                                                            <  1:611722/101‑1 (MQ=255)
            cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                                                           <  1:4641189/101‑1 (MQ=255)
            cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                                                           >  2:1609413/1‑101 (MQ=255)
             tGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcg                                                                                          <  1:445083/101‑1 (MQ=255)
              gCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccccc                                                                                         >  2:1946442/1‑99 (MQ=255)
               ccGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcgcg                                                                                        <  1:1456709/101‑1 (MQ=255)
               ccGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcgcg                                                                                        <  2:5119066/101‑1 (MQ=255)
                cggtgatcggtgatCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgg                                                                                       >  2:3436785/1‑101 (MQ=255)
                  gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg                                                                                     >  1:4009755/1‑101 (MQ=255)
                   tgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggggg                                                                                    >  1:1772931/1‑101 (MQ=255)
                   tgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggggg                                                                                    >  1:5062271/1‑101 (MQ=255)
                   tgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggggg                                                                                    <  1:2375281/101‑1 (MQ=255)
                   tgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCcgggg                                                                                    >  2:3984185/1‑101 (MQ=255)
                    gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggggg                                                                                   >  2:1326372/1‑100 (MQ=255)
                    gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                                                   <  2:174487/101‑1 (MQ=255)
                    gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                                                   <  2:2100303/101‑1 (MQ=255)
                    gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                                                   <  2:2696019/101‑1 (MQ=255)
                    gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                                                   <  2:1433974/101‑1 (MQ=255)
                     atcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCg                                                                                  <  1:129537/101‑1 (MQ=255)
                      tcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGt                                                                                 >  1:4784691/1‑101 (MQ=255)
                       cggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTg                                                                                <  2:3391719/101‑1 (MQ=255)
                         gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa                                                                              <  1:3809293/101‑1 (MQ=255)
                         gctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                             >  2:291142/10‑101 (MQ=2)
                          tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                             <  2:3000360/101‑1 (MQ=255)
                          ccgcacgtggTCGCCCCCCCCGCGGGGGCGTCGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                            <  1:1376366/93‑1 (MQ=0)
                            ggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                          >  2:3793831/7‑101 (MQ=2)
                            atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGCGTGGGTCCCGCCGCggggggcgggcgcg                                                                           >  2:2599967/1‑92 (MQ=25)
                              acgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCCCCCGGGGCTCTGGTC.ACGGTTTGGGAAGGAGCCGCTCCCCCCGCGGGGGCGTGGCTCgtcc                                                                        >  1:5707463/5‑98 (MQ=2)
                              acgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                        >  1:4342707/5‑101 (MQ=2)
                                gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                                      <  2:4584177/99‑1 (MQ=2)
                                gCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                       <  1:202891/101‑1 (MQ=255)
                                 cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                                      >  1:3682932/1‑101 (MQ=255)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                     >  1:5437641/1‑101 (MQ=255)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAAt                                                                     >  2:3930654/1‑101 (MQ=255)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtct                                                                   >  2:1560552/1‑101 (MQ=255)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtct                                                                   <  2:5773985/101‑1 (MQ=255)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtct                                                                   <  1:2980200/101‑1 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttc                                                                >  2:4703054/1‑98 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttc                                                                >  1:3497810/1‑98 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                                                 >  2:792827/1‑101 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                                                 <  2:5632324/101‑1 (MQ=255)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                                               <  1:3475614/101‑5 (MQ=2)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg.g                                                               <  2:688923/101‑1 (MQ=255)
                                         ccccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                                                             >  2:4824836/1‑95 (MQ=0)
                                         ccccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTg                                                             >  2:2870188/1‑101 (MQ=255)
                                          cccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGc                                                            <  1:804068/101‑1 (MQ=255)
                                           ccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGcc                                                           >  1:3170801/1‑101 (MQ=255)
                                            cccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCg                                                          <  2:4739575/101‑1 (MQ=255)
                                              cgcgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTc                                                        <  2:2023410/101‑1 (MQ=255)
                                                 gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCggt                                                     >  2:3572195/1‑99 (MQ=255)
                                                 gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCt                                                     >  1:1151582/1‑101 (MQ=255)
                                                  ggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTc                                                    >  2:1006178/1‑101 (MQ=255)
                                                  gcggCGTGGATCGAACCATCGCCACTATGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTc                                                    <  2:2649431/99‑1 (MQ=255)
                                                   gggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCg                                                   >  2:4109415/1‑101 (MQ=255)
                                                    ggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGt                                                  >  2:2451526/1‑101 (MQ=255)
                                                      cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGTATGCCGTcttctgct                                                 >  1:5293820/1‑94 (MQ=37)
                                                      cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTcc                                                <  2:5546908/101‑1 (MQ=255)
                                                        tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAg                                              <  1:2684305/101‑1 (MQ=255)
                                                           aTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCg                                           <  1:3367757/101‑1 (MQ=255)
                                                             cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGc                                         >  2:4165013/1‑101 (MQ=255)
                                                              gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGcc                                        >  1:1663205/1‑101 (MQ=255)
                                                               aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCt                                       >  2:2234151/1‑101 (MQ=255)
                                                               aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCt                                       >  1:2834114/1‑101 (MQ=255)
                                                                aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTg                                      <  1:1058147/101‑1 (MQ=255)
                                                                 aCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGGCTGc                                     >  2:1075162/1‑101 (MQ=255)
                                                                    tCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCtcg                                  <  2:815338/101‑1 (MQ=255)
                                                                      gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCtcgtc                                <  1:2747905/101‑1 (MQ=255)
                                                                         gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCt                             <  1:1586426/101‑1 (MQ=255)
                                                                          cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTc                            <  2:2834614/101‑1 (MQ=255)
                                                                            ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTccc                          >  2:4851943/1‑101 (MQ=255)
                                                                             tGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcccc                         <  1:423811/101‑1 (MQ=255)
                                                                                cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCcg                      >  1:5034750/1‑101 (MQ=255)
                                                                                  gTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCcgcg                    <  2:4555794/101‑1 (MQ=255)
                                                                                    cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCggg                  >  1:353716/1‑101 (MQ=255)
                                                                                    cGACAGGCTGGTAACGTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCggg                  <  2:4438236/101‑1 (MQ=255)
                                                                                     gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCgggg                 <  1:3634688/101‑1 (MQ=255)
                                                                                      aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCggggg                >  1:4586535/1‑101 (MQ=255)
                                                                                      aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCggggg                >  1:2289480/1‑101 (MQ=255)
                                                                                      aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCCCcgggg                >  1:4878154/1‑101 (MQ=255)
                                                                                       cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGc               <  1:2870349/101‑1 (MQ=255)
                                                                                        aGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCGCCCCCCGCGGGGGCg              <  2:3661282/101‑1 (MQ=255)
                                                                                          gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTg            <  1:2206726/101‑1 (MQ=255)
                                                                                          gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTg            <  1:5587719/101‑1 (MQ=255)
                                                                                          gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTg            <  1:944138/101‑1 (MQ=255)
                                                                                          gCTGGTAACGTGTCGCCCCCCCCGCGGGGTCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTg            <  1:5159793/101‑1 (MQ=255)
                                                                                            tGGTAGCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGa          <  1:4078514/101‑1 (MQ=255)
                                                                                            tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGa          >  2:5007977/1‑101 (MQ=255)
                                                                                             ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGAt         <  1:2451660/101‑1 (MQ=255)
                                                                                               tAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCg       <  2:2337974/101‑1 (MQ=255)
                                                                                                 aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     <  1:690549/101‑1 (MQ=255)
                                                                                                  cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa    >  1:3754592/1‑101 (MQ=255)
                                                                                                  cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa    <  1:1877176/101‑1 (MQ=255)
                                                                                                    tgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc  <  1:5538085/101‑1 (MQ=255)
                                                                                                    |                                                                                                     
CACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCG.GTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACC  >  NC_014034/1395399‑1395599

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG