Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,687 G→C 6.8% intergenic (+499/‑432) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6870G→C 6.8% 35.1 0.0 ‑0.4281intergenic (+499/‑432)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (10/9):  ref base (137/124):  total (147/134)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.65e-01

Only 100 of 317 total aligned reads displayed.

GTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1395587‑1395787
                                                                                                       |                                                                                                     
gTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAg                                                                                                       <  1:5201676/101‑1 (MQ=255)
gTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAg                                                                                                       <  1:4831910/101‑1 (MQ=255)
 tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGt                                                                                                      >  2:1232218/1‑101 (MQ=255)
  ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACGTCGGAATGTAGTCGGCG..G.GCAGtt                                                                                                     >  2:5400493/1‑101 (MQ=38)
   gATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATCTGCATGTAGTCGGCG..G.GCAGGTc                                                                                                    >  2:5043884/1‑101 (MQ=255)
   gATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTc                                                                                                    >  1:2943090/1‑101 (MQ=255)
   gATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTc                                                                                                    <  1:1951234/101‑1 (MQ=255)
       gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.Gc                                                                                               <  2:1267223/101‑1 (MQ=255)
        aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.Gcc                                                                                              >  1:79513/1‑101 (MQ=255)
        aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.Gcc                                                                                              >  1:5571891/1‑101 (MQ=255)
        aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.Gcc                                                                                              >  1:1791025/1‑101 (MQ=255)
         aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCg                                                                                             <  2:2218946/101‑1 (MQ=255)
         aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCg                                                                                             <  1:3962215/101‑1 (MQ=255)
            ccccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAg                                                                                          <  1:2407563/99‑1 (MQ=255)
             cccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGCCGGCG..G.GCAGTTCGA.GCCGCAGt                                                                                         <  2:3377254/100‑1 (MQ=255)
              ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTc                                                                                        <  2:1874184/101‑1 (MQ=255)
              ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTc                                                                                        <  1:4373143/101‑1 (MQ=255)
                   cGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTcc                                                                                   >  1:2100483/1‑101 (MQ=255)
                   cGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTcc                                                                                   >  2:4567470/1‑101 (MQ=255)
                   cGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTcc                                                                                   <  1:4120155/101‑1 (MQ=255)
                    ggATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTccc                                                                                  >  1:5401386/1‑101 (MQ=255)
                       ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTcccccc                                                                               <  1:1560646/101‑1 (MQ=255)
                       ttGTCACTGTCGCGGGGCGAAGCCTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTcccccc                                                                               <  2:3071973/101‑1 (MQ=255)
                        tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCcg                                                                              <  2:419156/101‑1 (MQ=255)
                        tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCACCCCCcg                                                                              >  1:978275/1‑101 (MQ=255)
                         gTCACTTTCGC.GGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCcgcg                                                                            >  2:398376/1‑101 (MQ=255)
                           cccTTTGGCGGGGCGAAGCGTCGCTCCCCCGGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgg                                                                           <  2:4745535/99‑1 (MQ=255)
                           cACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgg                                                                           >  1:1320060/1‑101 (MQ=255)
                               ttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGGAGTCGGCG..G.CCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGc                                                                       <  1:5299461/101‑1 (MQ=255)
                                 cgcgGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGt                                                                     <  1:4726597/101‑1 (MQ=255)
                                  tggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGt                                                                     <  2:64120/91‑1 (MQ=2)
                                  gcgGGGCGAGGCGTCGCGCCCCCCGCGGGGGCGGGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTg                                                                    <  1:3193946/101‑1 (MQ=255)
                                  gcgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTg                                                                    <  1:2892873/101‑1 (MQ=255)
                                   ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTg                                                                    >  2:1146911/10‑101 (MQ=2)
                                   ggtgccgctgCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTg                                                                    <  1:4506999/92‑1 (MQ=2)
                                   cgGGGCTAAGCGTCGCCCCCCCTGGGGGGACGTGGATCAAAACATCGGCATGTAGTCGGGG..G.GCAGTTCGA.GCCGCAGTCGCCCCCCCCGCGGGGGCGTgg                                                                   <  2:5559757/101‑1 (MQ=255)
                                    gtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                   <  1:268315/93‑1 (MQ=2)
                                    ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                  >  1:1412178/1‑101 (MQ=255)
                                     gggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 >  2:906044/1‑101 (MQ=255)
                                     gggCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 <  2:3592893/101‑1 (MQ=255)
                                      ggggAAGCGTCCCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  2:706801/98‑1 (MQ=255)
                                      ggCGAGGCGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  1:1019576/101‑1 (MQ=255)
                                      ggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  2:4450296/101‑1 (MQ=255)
                                      gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 <  2:2835973/95‑1 (MQ=2)
                                       ccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  2:5326537/96‑1 (MQ=2)
                                        cgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               >  2:4212932/5‑101 (MQ=2)
                                         gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                             <  2:34782/101‑1 (MQ=255)
                                         gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                             >  1:3453074/1‑101 (MQ=255)
                                          ctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCGGCAGTGTCA.GGTTTTCCA.GCGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                             <  1:3548070/99‑1 (MQ=2)
                                          ctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                             <  2:4929980/99‑1 (MQ=2)
                                           tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            >  1:1659929/2‑101 (MQ=2)
                                           tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            >  2:3324653/2‑101 (MQ=2)
                                           tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            >  2:2704099/2‑101 (MQ=2)
                                           tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            >  1:5459360/2‑101 (MQ=2)
                                             cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCCGAACTg                                                         >  2:5614574/1‑101 (MQ=255)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgg                                                        >  1:3709910/1‑101 (MQ=255)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgg                                                        >  2:3816160/1‑101 (MQ=255)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTAt                                                     >  1:1979828/1‑101 (MQ=255)
                                                 cccccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTAt                                                     <  2:2904973/98‑1 (MQ=255)
                                                  cccccccgGGGGGGGCGTGGATCGAAACATCGGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATg                                                    <  2:4320010/99‑1 (MQ=255)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                   >  2:2385341/1‑101 (MQ=255)
                                                    ccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGa                                                  >  1:2302457/1‑101 (MQ=255)
                                                     cccccGGGGGGGCGGGGATCGAAACATCTGCATGTGGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGAt                                                 <  1:5683637/101‑1 (MQ=255)
                                                     cccccGCGGGGGCCTGGATCCAAACCCCCGCATCTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGAt                                                 <  1:4919133/101‑1 (MQ=37)
                                                      ccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGGATGGATc                                                >  2:2065391/1‑101 (MQ=255)
                                                      ccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGGATGGATc                                                >  2:4017561/1‑101 (MQ=255)
                                                          gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                            >  1:5070293/1‑101 (MQ=255)
                                                          gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                            <  2:1410514/101‑1 (MQ=255)
                                                                gCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAAc                                      >  1:5721522/1‑101 (MQ=255)
                                                                   tGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                   >  1:579043/1‑101 (MQ=255)
                                                                   tGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                   >  2:5763105/1‑101 (MQ=255)
                                                                   tGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGGAGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                   >  2:5002243/1‑101 (MQ=255)
                                                                     gATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa                                 >  2:3893119/1‑101 (MQ=255)
                                                                      aTCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAg                                >  2:195141/1‑101 (MQ=255)
                                                                        cgcaaCATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                              >  1:3297113/4‑101 (MQ=255)
                                                                        cGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGGGCAAACGGAAAgcc                              >  2:1704518/1‑99 (MQ=255)
                                                                        cGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                              <  2:413936/101‑1 (MQ=255)
                                                                        cGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                              >  2:3220798/1‑101 (MQ=255)
                                                                         gAAACATCTGCATGTAGTCGGCG..G.GCAGTTGGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                             <  1:4154648/101‑1 (MQ=255)
                                                                         gAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                             >  2:664709/1‑101 (MQ=255)
                                                                          aaaCATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTg                            >  2:3181015/1‑101 (MQ=255)
                                                                           aaCATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                           >  1:3056047/1‑101 (MQ=255)
                                                                           aaCATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                           >  1:4311345/1‑101 (MQ=255)
                                                                           aaCATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                           >  2:4846339/1‑101 (MQ=255)
                                                                           aaCATCTGCATGTAGGCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                           <  1:1067295/101‑1 (MQ=255)
                                                                            aCATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtc                          <  1:1854577/101‑1 (MQ=255)
                                                                             cttCTGCATGTAGTCGGCG..G.GCAGTTCGA.GGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcg                         <  1:380268/99‑1 (MQ=255)
                                                                             cATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcg                         >  2:2551755/1‑101 (MQ=255)
                                                                              acctgctggTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt                        <  1:5232267/93‑1 (MQ=255)
                                                                               tCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc                       >  2:5309163/1‑101 (MQ=255)
                                                                                cTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGgcgtcg                      >  2:2000575/1‑101 (MQ=255)
                                                                                  gCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt                    <  2:4072575/101‑1 (MQ=255)
                                                                                   cATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTc                   >  1:2457145/1‑101 (MQ=255)
                                                                                   cATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTc                   >  1:536504/1‑101 (MQ=255)
                                                                                      gTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGGCTGTCGTCGCTcccc                >  2:2154292/1‑101 (MQ=255)
                                                                                        aGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc              >  2:4107372/1‑101 (MQ=255)
                                                                                          tgggcg..g.GCATTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcgc            <  2:5201928/99‑1 (MQ=255)
                                                                                            ggcg..g.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgg          >  1:3369135/1‑101 (MQ=255)
                                                                                                    gCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAAACGGGATGGGTCTTGGCCAAACGGAAAGGCCGTCGGCCCCCCCCCCCCcgggggg     >  1:2013780/1‑99 (MQ=12)
                                                                                                      aGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTg   >  1:4009694/1‑101 (MQ=255)
                                                                                                       |                                                                                                     
GTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1395587‑1395787

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG