Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,828 G→C 4.6% intergenic (+640/‑291) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8280G→C 4.6% 22.3 ‑0.9 ‑1.1285intergenic (+640/‑291)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/8):  ref base (141/131):  total (146/139)
Fisher's exact test strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.41e-02

Only 100 of 309 total aligned reads displayed.

AACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395728‑1395928
                                                                                                      |                                                                                                     
aaCTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTg                                                                                                       <  2:4070834/101‑1 (MQ=255)
  ccgatctGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGa                                                                                                     <  1:450340/95‑1 (MQ=255)
  cTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGa                                                                                                     >  2:3091211/1‑101 (MQ=255)
  cTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGa                                                                                                     <  1:4146816/101‑1 (MQ=255)
  cTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGa                                                                                                     >  2:2680446/1‑101 (MQ=255)
  cTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGa                                                                                                     <  2:2302331/101‑1 (MQ=255)
   tGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.c                                                                                                   <  1:136234/101‑1 (MQ=255)
   tGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.c                                                                                                   >  1:4858102/1‑101 (MQ=255)
    ggTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.Cg                                                                                                  <  2:2043389/101‑1 (MQ=255)
      tATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTc                                                                                                <  2:3055873/101‑1 (MQ=255)
         ggATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGaagatcgg                                                                                              >  2:450290/1‑94 (MQ=255)
            tCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTccc                                                                                          <  1:1258332/101‑1 (MQ=255)
             cTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccc                                                                                         <  1:1736450/101‑1 (MQ=255)
             cTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccc                                                                                         >  1:4361293/1‑101 (MQ=255)
             cTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccc                                                                                         <  2:3701831/101‑1 (MQ=255)
              tGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTccccc                                                                                        >  1:2566054/1‑101 (MQ=255)
               cgatctaaCGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccccc                                                                                       <  1:657254/95‑1 (MQ=255)
                 cgcAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcgc                                                                                     <  1:4109664/101‑1 (MQ=255)
                 cgcAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcgc                                                                                     >  2:3810500/1‑101 (MQ=255)
                 cgcAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcgc                                                                                     >  2:839918/1‑101 (MQ=255)
                  gcAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcccg                                                                                    >  1:3373228/1‑101 (MQ=255)
                   cAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgg                                                                                   <  1:2400696/101‑1 (MQ=255)
                   cAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgg                                                                                   >  1:1617412/1‑101 (MQ=255)
                   cAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgg                                                                                   >  2:5309472/1‑101 (MQ=255)
                     aaCGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTccccccagatcg                                                                                 >  2:657197/1‑95 (MQ=255)
                      aCGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCggggg                                                                                >  2:3444345/1‑101 (MQ=255)
                       cGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGc                                                                               <  2:5070009/101‑1 (MQ=255)
                        ggAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCg                                                                              <  1:1716095/101‑1 (MQ=255)
                         cggccccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCg                                                                              <  2:2903217/91‑1 (MQ=0)
                          ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt                                                                             >  1:5592627/10‑101 (MQ=0)
                          ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt                                                                             >  2:5267004/10‑101 (MQ=0)
                          aaaGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                            >  1:1023535/1‑101 (MQ=255)
                           ggcccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTg                                                                            <  2:369024/93‑1 (MQ=0)
                           gacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTg                                                                            <  1:2996774/93‑1 (MQ=0)
                           aaGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.GGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                                           >  1:4926827/1‑101 (MQ=255)
                           aaGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                                           <  1:5762172/101‑1 (MQ=255)
                            acccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGCgg                                                                           >  2:2528788/8‑101 (MQ=0)
                             gTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                         <  2:1033477/101‑1 (MQ=255)
                             gTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                         <  1:2176042/101‑1 (MQ=255)
                             gTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGGGGAt                                                                         >  2:5530636/1‑101 (MQ=255)
                              tCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  2:5663451/101‑1 (MQ=255)
                              tCTGTCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  2:4972843/101‑1 (MQ=255)
                                  tcgtcgCCCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                    <  2:5188700/101‑1 (MQ=255)
                                   cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                   >  1:5303001/1‑101 (MQ=255)
                                     tcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                                 <  2:3271056/101‑1 (MQ=255)
                                      cgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgc                                                                <  1:4695201/101‑1 (MQ=255)
                                       gcgccccccGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgcg                                                               <  1:2767737/98‑1 (MQ=255)
                                         tCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgcc                                                             <  2:1564768/101‑1 (MQ=255)
                                         tCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgcc                                                             >  2:3192613/1‑101 (MQ=255)
                                            ccccGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCgccgcc                                                          >  1:2342065/1‑101 (MQ=255)
                                             cccGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCt                                                         >  1:5164294/1‑101 (MQ=255)
                                              ccGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTa                                                        <  2:196753/101‑1 (MQ=255)
                                               cgcgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTAt                                                       >  2:2347524/1‑101 (MQ=255)
                                                gcgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATc                                                      <  1:3220998/101‑1 (MQ=255)
                                                gcgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATc                                                      <  2:1478883/101‑1 (MQ=255)
                                                 cgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGTTCGAAACTGCGCCGCCTATCg                                                     <  1:3290582/101‑1 (MQ=255)
                                                 cgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATcc                                                     >  2:4753159/1‑100 (MQ=255)
                                                 cgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTGCGCCGCCTATCg                                                     >  1:4041182/1‑101 (MQ=255)
                                                 cgGGGCCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGCGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTAtgg                                                     >  1:3892749/1‑99 (MQ=255)
                                                    gggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCAt                                                  >  1:5426897/1‑101 (MQ=255)
                                                     ggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATc                                                 <  2:2205020/101‑1 (MQ=255)
                                                     ggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATc                                                 >  1:3079630/1‑101 (MQ=255)
                                                      gCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATcc                                                >  1:1075071/1‑101 (MQ=255)
                                                      gCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATcc                                                <  1:3783248/101‑1 (MQ=255)
                                                       cGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCg                                               <  1:3816402/101‑1 (MQ=255)
                                                        gTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                                              <  2:4926546/101‑1 (MQ=255)
                                                         tGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCggc                                             >  2:3599438/1‑100 (MQ=255)
                                                          ggATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTc                                            >  2:4144951/1‑101 (MQ=255)
                                                           gATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCg                                           <  1:195161/101‑1 (MQ=255)
                                                           gATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCg                                           >  2:4623860/1‑101 (MQ=255)
                                                            aTCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGa                                          <  2:1710515/101‑1 (MQ=255)
                                                              cGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAgc                                        >  2:4270728/1‑101 (MQ=255)
                                                                aaaCCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAgcgc                                      >  2:3892335/1‑101 (MQ=255)
                                                                   ccTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGcgccgc                                   <  2:2111163/101‑1 (MQ=255)
                                                                    cTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCcgcg                                  <  2:4361027/101‑1 (MQ=255)
                                                                     tGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCgcgc                                 >  2:3133806/1‑101 (MQ=255)
                                                                       cg.gcgTGAGTTGCCAGTGATAGT.CGCCGTGGA.CGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAg                               <  2:4865900/101‑1 (MQ=255)
                                                                          gcgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGATCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTc                             <  1:695049/101‑1 (MQ=255)
                                                                           cgTGATTTGCCAGTTATAGC.CGCCGTGGA.CGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCg                            <  1:3957071/101‑1 (MQ=38)
                                                                           cgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCg                            <  1:2248935/101‑1 (MQ=255)
                                                                               aGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTcc                        >  2:2379561/1‑101 (MQ=255)
                                                                               aGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTcc                        <  1:3422423/101‑1 (MQ=255)
                                                                                       gTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCgg                >  2:781711/1‑101 (MQ=255)
                                                                                       gTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCgg                >  1:4304028/1‑101 (MQ=255)
                                                                                        tGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCggg               <  2:5419119/101‑1 (MQ=255)
                                                                                         gATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCgggg              <  1:839986/101‑1 (MQ=255)
                                                                                           tAGA.CGCCGTGGA.CGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGc            <  1:3722989/101‑1 (MQ=255)
                                                                                           tAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGNNNNNCGCGCAGTCGCTCCCCCCGCGGGGGc            >  1:4647209/1‑101 (MQ=255)
                                                                                           tAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGc            >  1:481414/1‑101 (MQ=255)
                                                                                             gA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGt          <  2:3368026/101‑1 (MQ=255)
                                                                                                 gCCGTGGC.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGa       <  2:1210814/101‑1 (MQ=255)
                                                                                                   cGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc     <  1:934930/101‑1 (MQ=255)
                                                                                                   cGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc     <  2:136783/101‑1 (MQ=255)
                                                                                                   cGTGGA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc     <  2:3296913/101‑1 (MQ=255)
                                                                                                    gTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg    >  1:773196/1‑101 (MQ=255)
                                                                                                    gTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg    <  1:3342083/101‑1 (MQ=255)
                                                                                                    gTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg    >  1:1427633/1‑101 (MQ=255)
                                                                                                    gTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg    >  1:1110430/1‑101 (MQ=255)
                                                                                                     tGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa   <  1:315860/101‑1 (MQ=255)
                                                                                                      ggA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  <  1:5548487/101‑1 (MQ=255)
                                                                                                      |                                                                                                     
AACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395728‑1395928

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG