Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,024 C→A 12.6% intergenic (+836/‑95) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,0240C→A12.6% 105.1 0.0 ‑0.0331intergenic (+836/‑95)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/23):  ref base (131/158):  total (150/181)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.96e-01

Only 100 of 362 total aligned reads displayed.

TCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAT  >  NC_014034/1395924‑1396124
                                                                                                      |                                                                                                       
 cGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGcc                                                                                                        <  2:2056968/101‑1 (MQ=255)
  gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCt                                                                                                       <  1:2598521/101‑1 (MQ=255)
  gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCt                                                                                                       <  2:2886886/101‑1 (MQ=255)
   aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTg                                                                                                      >  1:4166521/1‑101 (MQ=255)
    aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGc                                                                                                     >  1:4499520/1‑101 (MQ=255)
    aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGc                                                                                                     >  1:5784933/1‑101 (MQ=255)
     aCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGc.c                                                                                                   <  2:175315/101‑1 (MQ=255)
       gATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.cgc                                                                                                 <  1:2132675/101‑1 (MQ=255)
        aTGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.cgc..g                                                                                              <  1:2963119/101‑1 (MQ=255)
         tGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..Gt                                                                                             <  2:3759686/101‑1 (MQ=255)
         tGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..Gt                                                                                             >  2:3471322/1‑101 (MQ=255)
           cGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCg                                                                                           <  1:781779/101‑1 (MQ=255)
              tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTc                                                                                        >  2:2696664/1‑101 (MQ=255)
                aTGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTccc                                                                                      >  2:1637258/1‑101 (MQ=255)
                   gCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTTCC..TCACCCCTGC.CGC..GTCGCTcccccc                                                                                   >  1:5117047/1‑101 (MQ=255)
                   gCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTcccccc                                                                                   <  2:3079456/101‑1 (MQ=255)
                    ccTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCcg                                                                                  >  2:3854736/1‑101 (MQ=255)
                    ccTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCcg                                                                                  <  2:4303763/101‑1 (MQ=255)
                    ccTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCcg                                                                                  <  1:2696813/101‑1 (MQ=255)
                      tACGTCGGGCAGTGGATTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCcgcg                                                                                >  2:1212264/1‑101 (MQ=255)
                         gTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCgggg                                                                             <  1:3242463/101‑1 (MQ=255)
                           cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGc                                                                           >  1:3645473/1‑101 (MQ=255)
                           cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGc                                                                           <  2:3327705/101‑1 (MQ=255)
                            gggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCg                                                                          >  2:748416/1‑101 (MQ=255)
                             atctgTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCCCGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGt                                                                         <  1:1444162/97‑1 (MQ=255)
                                aggggccTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAACCTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                                      <  1:1007181/95‑1 (MQ=38)
                                aGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                                      >  1:2396185/1‑101 (MQ=255)
                                 gTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                     <  2:2314819/101‑1 (MQ=255)
                                 gTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTAGAt                                                                     >  2:1444069/1‑101 (MQ=255)
                                  tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                    <  1:5530928/101‑1 (MQ=255)
                                  tGGACGGGTCGCGCCCCCCGCGGGGTCGTGGATCTAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                    <  2:870029/101‑1 (MQ=38)
                                  ctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                     <  1:3011103/95‑1 (MQ=2)
                                   ttcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                    <  1:1350890/96‑1 (MQ=9)
                                   tgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                    <  2:2231940/96‑1 (MQ=2)
                                   ggcgcggTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                    <  1:630716/96‑1 (MQ=2)
                                   ggACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                   >  2:3658219/1‑101 (MQ=255)
                                     aCTGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                 <  1:2493161/101‑1 (MQ=255)
                                     aCTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                 <  1:4371265/101‑1 (MQ=255)
                                     aCTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                 <  2:5164081/101‑1 (MQ=255)
                                      gcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                 <  2:5585802/99‑1 (MQ=2)
                                      cTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                <  2:2434526/101‑1 (MQ=255)
                                        ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                              >  2:2972729/1‑101 (MQ=255)
                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActt                                                             <  1:1981962/101‑3 (MQ=2)
                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActt                                                             <  2:1959601/101‑3 (MQ=2)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgc                                                           >  1:5436433/1‑101 (MQ=255)
                                             gcccccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCCCGTATTGCGCGC.TG...GTCGCTCCCCCCGCGGGGGCGGGGATCGAAActtgat                                                          >  1:4106998/3‑96 (MQ=2)
                                             cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgat                                                          <  2:338103/101‑6 (MQ=2)
                                              tCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCa                                                        <  1:4348258/101‑1 (MQ=255)
                                              tCCCCCCGCGGGGGCGTGGATCGAAACTCGCACTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCa                                                        >  1:5165462/1‑101 (MQ=255)
                                               ccccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatga                                                        <  1:161132/101‑8 (MQ=2)
                                               ccccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatga                                                        >  1:1508549/1‑94 (MQ=2)
                                               ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                       <  1:5634218/101‑1 (MQ=255)
                                                cccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgac                                                       >  1:4306250/1‑93 (MQ=2)
                                                cccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATc                                                      >  2:50999/1‑101 (MQ=255)
                                                 ccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                                                      >  2:2092905/1‑92 (MQ=2)
                                                 ccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                                                      <  1:3405446/101‑10 (MQ=2)
                                                 ccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCt                                                     <  2:4848341/101‑1 (MQ=255)
                                                       gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                               >  1:3841964/1‑101 (MQ=255)
                                                        ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                                              >  1:5413820/1‑101 (MQ=255)
                                                        ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                                              <  1:457218/101‑1 (MQ=255)
                                                        ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                                              >  2:3927971/1‑101 (MQ=255)
                                                         gggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtata                                             <  2:4209748/101‑1 (MQ=255)
                                                           gCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTACATg                                           <  2:4908386/101‑1 (MQ=255)
                                                            cGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGc                                          >  2:652216/1‑101 (MQ=255)
                                                            cGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGc                                          >  2:2354337/1‑101 (MQ=255)
                                                             gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                                         >  1:3291325/1‑101 (MQ=255)
                                                              tGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGccc                                        >  2:5501937/1‑101 (MQ=255)
                                                              tGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGccc                                        >  1:5245079/1‑101 (MQ=255)
                                                              tGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGccc                                        >  1:482373/1‑101 (MQ=255)
                                                                    gAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                                  <  2:5278655/101‑1 (MQ=255)
                                                                    caaaCCCGCCCTCGATCCGCGCTTGCC..TCCCGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                                  <  1:1075237/100‑1 (MQ=255)
                                                                     aaaCTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCTGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                                 <  2:5005061/101‑1 (MQ=255)
                                                                     aaaCTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                                 <  2:5784638/101‑1 (MQ=255)
                                                                     aaaCTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                                 <  2:53598/101‑1 (MQ=255)
                                                                      aaCTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGAc                                >  1:3885628/1‑101 (MQ=255)
                                                                      aaCTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGAc                                <  1:1312119/101‑1 (MQ=255)
                                                                       aCTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACg                               >  1:2060887/1‑101 (MQ=255)
                                                                       aCTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACg                               >  1:1160518/1‑101 (MQ=255)
                                                                         tCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGc                             <  1:4904033/101‑1 (MQ=255)
                                                                          cGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCt                            <  2:1582880/101‑1 (MQ=255)
                                                                            cccTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGt                          <  1:1868232/101‑1 (MQ=255)
                                                                             ccTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTc                         <  2:5634478/101‑1 (MQ=255)
                                                                                agATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAGACAGGGGCATCTGATGTATATGCCCGGGGAGACGGCTGTCGCt                      >  2:1354618/2‑101 (MQ=255)
                                                                                 gATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTc                     >  2:3284934/1‑101 (MQ=255)
                                                                                  aTCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcc                    >  1:478581/1‑101 (MQ=255)
                                                                                  aTCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcc                    >  2:5443157/1‑101 (MQ=255)
                                                                                    ccGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccc                  >  2:4435855/1‑101 (MQ=255)
                                                                                      gcgcTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccccc                <  1:888500/101‑1 (MQ=255)
                                                                                      gcgcTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccccc                <  2:1729951/101‑1 (MQ=255)
                                                                                      gcgcTTGCC..TCACGCCTGC.CAC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccccc                <  2:4069357/101‑1 (MQ=255)
                                                                                       cgcTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcg               <  1:4067869/101‑1 (MQ=255)
                                                                                         cTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcgcg             >  2:5218864/1‑101 (MQ=255)
                                                                                          ttGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCgg            >  1:2778846/1‑101 (MQ=255)
                                                                                            gCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCgggg          >  2:3711477/1‑101 (MQ=255)
                                                                                              c..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGc        >  1:225422/1‑101 (MQ=255)
                                                                                              c..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGc        <  2:1074996/101‑1 (MQ=255)
                                                                                              c..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGc        >  2:1306994/1‑101 (MQ=255)
                                                                                              c..TCACGCCTGC.CGC..GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGc        <  1:3471539/101‑1 (MQ=255)
                                                                                                     gCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGa   >  1:3142915/1‑101 (MQ=255)
                                                                                                      ccTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAt  <  2:2526076/101‑1 (MQ=255)
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TCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAT  >  NC_014034/1395924‑1396124

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG