Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,478 A→C 6.3% E120D (GAA→GAC RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,4780A→C 6.3% 37.0 0.0 ‑0.1287E120D (GAA→GACRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/9):  ref base (135/134):  total (144/143)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.87e-01

Only 100 of 302 total aligned reads displayed.

CCCCGCGGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGC  >  NC_014034/1396378‑1396577
                                                                                                    |                                                                                                    
ccccGCGGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGaa                                                                                                      <  2:3494631/101‑1 (MQ=255)
 cccGCGGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGaaa                                                                                                     <  1:4801457/101‑1 (MQ=255)
  ccGCGGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAAt                                                                                                    >  1:3767459/1‑101 (MQ=255)
    gcgGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTc                                                                                                  >  2:3995689/1‑101 (MQ=255)
    gcgGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTc                                                                                                  >  1:2098605/1‑101 (MQ=255)
     cgGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTcc                                                                                                 >  2:286829/1‑101 (MQ=255)
      gggggCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCcg                                                                                                >  1:4473380/1‑101 (MQ=255)
        gggCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCcgcg                                                                                              >  1:4168760/1‑101 (MQ=255)
           cGTGGATCGAAACCGCGCCCTGCGGGTGATTGCGACGGGCGCCGCGGCGGTCGCTCCCCCCACGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGa                                                                                           <  2:4791446/101‑1 (MQ=38)
           cGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGa                                                                                           >  2:1506795/1‑101 (MQ=255)
           cGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGa                                                                                           >  2:2452375/1‑101 (MQ=255)
           cGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGa                                                                                           >  2:2653636/1‑101 (MQ=255)
            gTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGaa                                                                                          <  2:2507326/101‑1 (MQ=255)
              ggATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAAc                                                                                        >  1:3624284/1‑101 (MQ=255)
              ggATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAAc                                                                                        >  1:3151994/1‑101 (MQ=255)
                aTCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAAcgc                                                                                      >  1:1807076/1‑101 (MQ=255)
                       ccGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGAGGATCGAAACGGACCTGAAATTCCGCGGGAAACgcggcggcg                                                                               <  2:2951753/101‑1 (MQ=255)
                        cgcgCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGa                                                                              >  2:3319818/1‑101 (MQ=255)
                          cgcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGc                                                                            >  1:2544521/1‑101 (MQ=255)
                          cgcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGc                                                                            <  1:5420392/101‑1 (MQ=255)
                           gcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCa                                                                           >  2:876146/1‑101 (MQ=255)
                            cccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAc                                                                          <  1:1690637/101‑1 (MQ=255)
                            cccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAc                                                                          <  1:3903971/101‑1 (MQ=255)
                             ccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.g                                                                        >  2:2280675/1‑101 (MQ=255)
                              cTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGGAC.Gt                                                                       >  2:1056341/1‑101 (MQ=255)
                               tGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTc                                                                      <  2:238762/101‑1 (MQ=255)
                                gCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCg                                                                     >  2:394994/1‑101 (MQ=255)
                                gCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCg                                                                     <  2:3537990/101‑1 (MQ=255)
                                  gggTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCt                                                                   <  2:3596223/101‑1 (MQ=255)
                                  gggTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCt                                                                   >  1:1486652/1‑101 (MQ=255)
                                  gggAGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCt                                                                   >  1:2511098/1‑101 (MQ=255)
                                   ggTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTc                                                                  <  1:1935844/101‑1 (MQ=255)
                                    gTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcc                                                                 <  2:1830318/101‑1 (MQ=255)
                                    gTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcc                                                                 <  2:1941644/101‑1 (MQ=255)
                                     tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccc                                                                <  2:3151805/101‑1 (MQ=255)
                                     tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccc                                                                >  2:3531174/1‑101 (MQ=255)
                                       aTGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccccc                                                              <  2:2166595/101‑1 (MQ=255)
                                       aTGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccccc                                                              <  2:3872435/101‑1 (MQ=255)
                                        tGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccc                                                             <  2:1272988/101‑1 (MQ=255)
                                        tGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccc                                                             <  1:2953864/101‑1 (MQ=255)
                                        tGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccc                                                             >  2:5308675/1‑101 (MQ=255)
                                                 gCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCg                                                    >  2:2992928/1‑101 (MQ=255)
                                                  caccacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGt                                                   >  1:3600019/1‑101 (MQ=255)
                                                   accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                                  <  2:4048819/101‑1 (MQ=255)
                                                     cacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                <  1:2390703/101‑1 (MQ=255)
                                                      tgcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATc                                              <  1:5232659/95‑1 (MQ=2)
                                                      acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                               >  1:5004601/1‑101 (MQ=255)
                                                      acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                               <  1:830254/101‑1 (MQ=255)
                                                      acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                               >  2:2093567/1‑101 (MQ=255)
                                                        ggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                             >  1:3560250/1‑101 (MQ=255)
                                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                          >  1:3408052/1‑101 (MQ=255)
                                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                          >  1:203906/1‑101 (MQ=255)
                                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                       >  1:5077920/1‑101 (MQ=255)
                                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                       >  2:1412244/1‑101 (MQ=255)
                                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.CTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                       >  1:4937886/1‑101 (MQ=255)
                                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgct                                      >  1:979100/1‑98 (MQ=2)
                                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                     >  1:5586094/1‑97 (MQ=0)
                                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                     <  2:4952825/101‑5 (MQ=0)
                                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                     >  2:167330/1‑97 (MQ=0)
                                                                 tCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCtt                                    >  2:2011870/1‑101 (MQ=255)
                                                                 tCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCtt                                    >  1:1763691/1‑101 (MQ=255)
                                                                 tCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctggc                                   <  1:1299346/101‑7 (MQ=0)
                                                                 tCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctggc                                   >  1:4283566/1‑95 (MQ=0)
                                                                   cccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                                  <  1:4381148/101‑1 (MQ=255)
                                                                   cccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                                  <  2:5234477/101‑1 (MQ=255)
                                                                   cccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                                  <  1:2791071/101‑1 (MQ=255)
                                                                     cccGCGGGGGCGTGGAGCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCTGCGAGCAC.GTCTCTCCCCCCGGTGGTGGGTTGATCGAAACTGCTTTCGt                                <  2:1776629/101‑1 (MQ=255)
                                                                       cgcgGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTgg                              >  1:3360388/1‑101 (MQ=255)
                                                                       cgcgGGGGCGTGGATCGAAACGCACCTGAAATTCCGCAGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTgg                              <  2:5738044/101‑1 (MQ=255)
                                                                         cgGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGa                            >  1:4875878/1‑101 (MQ=255)
                                                                         cgGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGa                            <  1:4666341/101‑1 (MQ=255)
                                                                          gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTGCTTTCGTGGGAc                           >  1:1079201/1‑101 (MQ=255)
                                                                          gggagCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGAc                           >  1:3495556/1‑101 (MQ=255)
                                                                            gggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTg                         <  1:1377635/101‑1 (MQ=255)
                                                                            gggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTg                         >  1:5558581/1‑101 (MQ=255)
                                                                            gggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTg                         <  1:5195024/101‑1 (MQ=255)
                                                                             ggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTgg                        <  2:5522262/101‑1 (MQ=255)
                                                                               cGTNGNTCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGcc                      <  1:1538760/101‑1 (MQ=255)
                                                                               cGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGcc                      >  1:160332/1‑101 (MQ=255)
                                                                               cGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGcc                      >  1:3215546/1‑101 (MQ=255)
                                                                               cGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGcc                      >  2:1518151/1‑101 (MQ=255)
                                                                                gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCa                     <  2:3789165/101‑1 (MQ=255)
                                                                                 tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg                    <  2:4656268/101‑1 (MQ=255)
                                                                                 tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg                    >  1:5651239/1‑101 (MQ=255)
                                                                                   gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgcc                  >  2:4238463/1‑101 (MQ=255)
                                                                                   gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACAGGCCAgcc                  >  2:5639341/1‑101 (MQ=255)
                                                                                      cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccgcc               >  2:647808/1‑101 (MQ=255)
                                                                                      cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccgcc               <  1:459103/101‑1 (MQ=255)
                                                                                       gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccgccg              <  2:5077635/101‑2 (MQ=255)
                                                                                        aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccg             >  1:73115/1‑101 (MQ=255)
                                                                                         aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccgc            <  1:2993095/101‑1 (MQ=255)
                                                                                         aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccgc            >  2:4287878/1‑101 (MQ=255)
                                                                                           cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCgcgc          >  1:2430833/1‑101 (MQ=255)
                                                                                            ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGcc         <  2:4102540/101‑1 (MQ=255)
                                                                                              aCCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCaa       <  1:2865200/101‑1 (MQ=255)
                                                                                               ccTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAg      >  2:771161/1‑101 (MQ=255)
                                                                                               ccTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAg      <  1:3803614/101‑1 (MQ=255)
                                                                                                cTGAAATTCCGCGGGAAGCGCGGCGGCGAGCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGt     <  2:4473108/101‑1 (MQ=255)
                                                                                                 tGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTc    <  1:2280801/101‑1 (MQ=255)
                                                                                                  gAAATTCCGCGGGATCCCCGGCGGCGAGCAC.CTCGCTCCCCCCGCGGTGGCGTAGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCg   <  1:666932/101‑1 (MQ=38)
                                                                                                    |                                                                                                    
CCCCGCGGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGC  >  NC_014034/1396378‑1396577

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG