Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,539 T→A 13.7% C141S (TGC→AGC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5390T→A13.7% 134.1 ‑1.9 ‑1.0329C141S (TGC→AGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/26):  ref base (156/128):  total (175/154)
Fisher's exact test strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.94e-02

Only 100 of 350 total aligned reads displayed.

CGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGG  >  NC_014034/1396440‑1396639
                                                                                                   |                                                                                                      
cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                                                                       >  2:1412244/1‑101 (MQ=255)
  cTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCt                                                                                                     >  1:3352975/1‑101 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCttt                                                                                                   <  2:893194/101‑1 (MQ=255)
     cccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                                                                                                  <  1:2791071/101‑1 (MQ=255)
     cccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                                                                                                  <  1:4381148/101‑1 (MQ=255)
     cccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                                                                                                  <  2:2184225/101‑1 (MQ=255)
           cgGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGa                                                                                           >  1:2069896/1‑101 (MQ=255)
            gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGAc                                                                                          >  2:4052851/1‑101 (MQ=255)
            gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGAc                                                                                          <  2:4471494/101‑1 (MQ=255)
            gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGAc                                                                                          >  2:1964715/1‑101 (MQ=255)
            gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTGCTTTC.GTGGGAc                                                                                          >  1:1079201/1‑101 (MQ=255)
            gggagCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGAc                                                                                          >  1:3495556/1‑101 (MQ=255)
              gggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTg                                                                                        >  2:1269725/1‑101 (MQ=255)
               ggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTgg                                                                                       <  2:5522262/101‑1 (MQ=255)
                 cGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGcc                                                                                     >  1:160332/1‑101 (MQ=255)
                 cGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACACGGCGGCGAGCACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GGGGGCCTGGcc                                                                                     >  1:1295907/1‑101 (MQ=38)
                  gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCa                                                                                    <  2:2505733/101‑1 (MQ=255)
                  gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCa                                                                                    >  2:3516208/1‑101 (MQ=255)
                   tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAg                                                                                   >  1:5651239/1‑101 (MQ=255)
                   tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAg                                                                                   <  2:4656268/101‑1 (MQ=255)
                     gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgcc                                                                                 >  2:5125424/1‑101 (MQ=255)
                     gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGACGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgcc                                                                                 <  2:5235006/101‑1 (MQ=255)
                      aTCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgccg                                                                                >  1:3208941/1‑101 (MQ=255)
                        cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgccgcc                                                                              <  1:459103/101‑1 (MQ=255)
                         gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccc                                                                             >  1:4870810/1‑101 (MQ=255)
                          aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccg                                                                            >  1:177192/1‑101 (MQ=255)
                           aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccgc                                                                           <  1:2993095/101‑1 (MQ=255)
                           aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccgc                                                                           >  2:4287878/1‑101 (MQ=255)
                           aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccgc                                                                           >  2:5770246/1‑101 (MQ=255)
                             cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCgcg.c                                                                        >  1:2430833/1‑101 (MQ=255)
                              ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.cc                                                                       <  2:4102540/101‑1 (MQ=255)
                                 ccTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAg                                                                    <  1:3803614/101‑1 (MQ=255)
                                  cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGt                                                                   <  1:523379/101‑1 (MQ=255)
                                    gAAATTCCGCGGGATCCCCGGCGGCGAGCACCTCGCTCCCCCCGCGGTGGCGTAGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCg                                                                 <  1:666932/101‑1 (MQ=38)
                                     aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGc                                                                >  2:3227789/1‑101 (MQ=255)
                                       aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTc                                                              <  1:5511661/101‑1 (MQ=255)
                                          ccGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTcccc                                                           <  1:4190277/101‑1 (MQ=255)
                                          ccGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTGCTTTC.GTGGGACTGGCCCGCCGCCCGCG.CCAAGTCGCTcccc                                                           >  2:4522751/1‑101 (MQ=255)
                                           cgcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTccccc                                                          <  1:4635755/101‑1 (MQ=255)
                                            gcgGGAACCGCGGCGGCGAGCACGTCGCTCCCCGCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTcccccc                                                         <  1:4984703/101‑1 (MQ=255)
                                            gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTcccccc                                                         <  1:2861442/101‑1 (MQ=255)
                                            gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTcccccc                                                         >  1:1037161/1‑101 (MQ=255)
                                             cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCcg                                                        >  1:2952184/1‑101 (MQ=255)
                                              gggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCcgc                                                       >  2:1835181/1‑101 (MQ=255)
                                              gggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCcgc                                                       >  1:1969721/1‑101 (MQ=255)
                                              gggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCCCCCACcgc                                                       >  2:1025241/1‑101 (MQ=255)
                                                  aaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCgggg                                                   >  1:4905640/1‑101 (MQ=255)
                                                    cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGc                                                 <  1:1515729/101‑1 (MQ=255)
                                                     gcggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCg                                                >  2:713690/1‑101 (MQ=255)
                                                      cggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCgg                                               >  2:2375702/1‑100 (MQ=255)
                                                         ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAAC.CTATGTCGCTCACCCCGCGGGGGCGTgg                                             <  2:3645236/91‑1 (MQ=2)
                                                         cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGGCTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                            <  1:2930425/101‑1 (MQ=255)
                                                          tgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAAC.CTATGTCGCTCACCCCGCGGGGGCGTGGa                                            <  2:2893959/92‑1 (MQ=2)
                                                           gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGGGGATc                                          >  1:297178/1‑101 (MQ=255)
                                                            cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATca                                         <  2:4765783/101‑2 (MQ=255)
                                                            cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  1:84896/1‑101 (MQ=255)
                                                             gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        >  2:1752952/1‑101 (MQ=255)
                                                              aGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       <  2:4562825/101‑1 (MQ=255)
                                                               gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  2:2960415/101‑1 (MQ=255)
                                                                ggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAAC.CTATGTCGCTCACCCCGCGGGGGCGTGGATCGaaa                                      >  2:1358472/4‑101 (MQ=2)
                                                                caggTCACTCCCGCCGCGGGGGGGGGAATCGAAACTGATTAC.GTGGGCCTGGCCACCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                     <  2:4862953/98‑1 (MQ=21)
                                                                cACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                     <  2:679546/101‑1 (MQ=255)
                                                                 aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                    <  2:3471147/101‑1 (MQ=255)
                                                                 aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCCCGGGGGCGTGGATCGAAACg                                    >  2:2563958/1‑101 (MQ=255)
                                                                  cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                   <  2:2069779/101‑1 (MQ=255)
                                                                  agTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAAC.CTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAAct                                    <  2:824868/100‑2 (MQ=2)
                                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAAC.CTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                 >  1:2177471/1‑97 (MQ=2)
                                                                      gccccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                               >  1:761690/4‑101 (MQ=255)
                                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                               >  1:3986523/1‑101 (MQ=255)
                                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAAC.CTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgc                                >  1:941821/1‑96 (MQ=2)
                                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAAC.CTATGTCGCTCACCCCGCGGGGGCGTCGATCGAAActgtgc                                >  1:5099152/1‑96 (MQ=2)
                                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcg                              >  2:1537911/1‑101 (MQ=255)
                                                                        tCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAAC.CTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccg                              >  2:1114806/1‑94 (MQ=2)
                                                                         ccccccGCGGGGGCGTGGNTCGAAAC.GANANC.GGGGCTCTGGTCA.CGGTTTGNGACGNAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcagctttcaat                           <  1:1393024/101‑11 (MQ=2)
                                                                         ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAAC.CTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                             <  1:3640680/101‑9 (MQ=2)
                                                                          cccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtcg                           >  1:1944321/1‑101 (MQ=255)
                                                                          cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAAC.CTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                            <  1:5051291/101‑10 (MQ=2)
                                                                           ccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGc                          >  2:3069464/1‑101 (MQ=255)
                                                                           ccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAAC.CTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggtt                           <  2:3333292/101‑11 (MQ=2)
                                                                            cccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCt                         <  2:5587915/101‑1 (MQ=255)
                                                                            cccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCt                         >  1:255653/1‑101 (MQ=255)
                                                                             ccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                        <  2:5650944/101‑1 (MQ=255)
                                                                              cgcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                       >  1:5424326/1‑101 (MQ=255)
                                                                              cgcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                       <  2:4937605/101‑1 (MQ=255)
                                                                               gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                      >  1:5146712/1‑101 (MQ=255)
                                                                               gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                      <  2:3792378/101‑1 (MQ=255)
                                                                                cgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacg                     >  1:1459188/1‑101 (MQ=255)
                                                                                   gggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgacg                  <  1:1835291/101‑1 (MQ=255)
                                                                                    ggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGGCGCTTacgaccc                 >  1:4279599/1‑99 (MQ=255)
                                                                                       gTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa              <  2:5035368/101‑1 (MQ=255)
                                                                                         ggATCGAAACTGCTTTC.GTGGGACTGACCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc            <  2:3675600/101‑1 (MQ=255)
                                                                                          gATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt           <  1:35182/101‑1 (MQ=255)
                                                                                          gATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt           >  1:2472394/1‑101 (MQ=255)
                                                                                           aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg          >  2:3929957/1‑101 (MQ=255)
                                                                                           aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg          >  2:5795011/1‑101 (MQ=255)
                                                                                            tCGAACATGCTTTC.GTGGGACTGGCCGGCGGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGa         <  2:3560092/101‑1 (MQ=38)
                                                                                             cGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt        <  1:1713346/101‑1 (MQ=255)
                                                                                             cGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt        >  1:2366806/1‑101 (MQ=255)
                                                                                               aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt      >  2:2733531/1‑101 (MQ=255)
                                                                                                aaCTGCTTTC.GTGGGACTGGCCAGCCGCACGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTc     <  1:5308965/101‑1 (MQ=255)
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CGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGG  >  NC_014034/1396440‑1396639

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG