Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,559 G→C 14.3% Q147H (CAG→CGC RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5590G→C14.3% 105.3 ‑2.6 ‑0.1312Q147H (CAG→CACRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (18/27):  ref base (146/121):  total (164/148)
Fisher's exact test strand distribution p-value = 7.69e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.51e-01

Only 100 of 354 total aligned reads displayed.

TGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCG  >  NC_014034/1396459‑1396659
                                                                                                     |                                                                                                     
tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAg                                                                                                       >  1:441704/1‑101 (MQ=255)
tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAg                                                                                                       <  1:2452509/101‑1 (MQ=255)
  gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgcc                                                                                                     >  2:2388400/1‑101 (MQ=255)
  gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgcc                                                                                                     >  2:4238463/1‑101 (MQ=255)
  gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgcc                                                                                                     >  2:5125424/1‑101 (MQ=255)
  gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGACGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgcc                                                                                                     <  2:5235006/101‑1 (MQ=255)
   aTCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgccg                                                                                                    <  2:3624056/101‑1 (MQ=255)
     cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgccgcc                                                                                                  <  1:459103/101‑1 (MQ=255)
       aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccg                                                                                                >  1:73115/1‑101 (MQ=255)
       aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccg                                                                                                >  1:177192/1‑101 (MQ=255)
          cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCgcg.c                                                                                            >  1:2536103/1‑101 (MQ=255)
          cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCgcg.c                                                                                            >  1:2430833/1‑101 (MQ=255)
               cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGt                                                                                       <  1:523379/101‑1 (MQ=255)
                tGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTc                                                                                      <  1:2280801/101‑1 (MQ=255)
                  aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGc                                                                                    >  2:3227789/1‑101 (MQ=255)
                     ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTcc                                                                                 >  1:3747013/1‑101 (MQ=255)
                      tCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTccc                                                                                >  2:2963900/1‑101 (MQ=255)
                       ccGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTcccc                                                                               <  1:4190277/101‑1 (MQ=255)
                       ccGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTGCTTTC.GTGGGACTGGCCCGCCGCCCGCG.CCAAGTCGCTcccc                                                                               >  2:4522751/1‑101 (MQ=255)
                         gcgGGAACCGCGGCGGCGAGCACGTCGCTCCCCGCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTcccccc                                                                             <  1:4984703/101‑1 (MQ=255)
                         gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTcccccc                                                                             >  1:1037161/1‑101 (MQ=255)
                         gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTcccccc                                                                             <  1:2861442/101‑1 (MQ=255)
                         gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTcccccc                                                                             <  1:425523/101‑1 (MQ=255)
                           gggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCcgc                                                                           >  1:1969721/1‑101 (MQ=255)
                             gAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCgg                                                                         >  2:2854627/1‑101 (MQ=255)
                              aaaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCggg                                                                        >  1:1107208/1‑101 (MQ=255)
                              aaaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCggg                                                                        >  1:3953004/1‑101 (MQ=255)
                                  gcggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCg                                                                    >  2:713690/1‑101 (MQ=255)
                                  gcggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCg                                                                    >  1:3034521/1‑101 (MQ=255)
                                      cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                >  2:5578806/1‑101 (MQ=255)
                                       ggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                               >  2:5738638/1‑101 (MQ=255)
                                       ggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                               <  2:2906715/101‑1 (MQ=255)
                                         ggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAAC.CTATGTCGCTCACCCCGCGGGGGCGTGGATc                                                              <  1:1435881/94‑1 (MQ=2)
                                         cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATca                                                             <  2:4765783/101‑2 (MQ=255)
                                         cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             >  2:4595277/1‑101 (MQ=255)
                                         cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  2:203886/101‑1 (MQ=255)
                                          gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            >  2:1752952/1‑101 (MQ=255)
                                          gAGCACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            <  2:3560033/101‑1 (MQ=255)
                                           aGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           <  2:4562825/101‑1 (MQ=255)
                                            cggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAAC.CTATGTCGCTCACCCCGCGGGGGCGTGGATCGaa                                                           <  2:2682152/97‑1 (MQ=2)
                                             cgcGTCGCTCGCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAAC.CTATGTCGCTCACCCCGCGGGGGCGTGGATCGaaa                                                          <  2:4313641/99‑1 (MQ=2)
                                             caggTCACTCCCGCCGCGGGGGGGGGAATCGAAACTGATTAC.GTGGGCCTGGCCACCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                         <  2:4862953/98‑1 (MQ=21)
                                             cACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                         >  1:482894/1‑101 (MQ=255)
                                              gagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAAC.CTATGTCGCTCACCCCGCGGGGGCGTGGGTCGAAAc                                                         >  2:8490/3‑101 (MQ=2)
                                              aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                        <  2:3471147/101‑1 (MQ=255)
                                              aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                        >  1:880009/1‑101 (MQ=255)
                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                      >  1:1929139/1‑101 (MQ=255)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                                    <  1:2964067/101‑1 (MQ=255)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                                    >  1:1474093/1‑101 (MQ=255)
                                                  cGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGCCTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                                    <  2:1347690/101‑1 (MQ=255)
                                                    cTCCCCCGGCGGGGGCGTGGATCGAAACTTCTTTC.GTGGGACTGGCCACCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcg                                                  <  1:2932799/101‑1 (MQ=255)
                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcg                                                  <  2:5647171/101‑1 (MQ=255)
                                                      ccccccGCGGGGGCGTGGNTCGAAAC.GANANC.GGGGCTCTGGTCA.CGGTTTGNGACGNAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcagctttcaat                                               <  1:1393024/101‑11 (MQ=2)
                                                      ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                <  2:3325059/101‑1 (MQ=255)
                                                       cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAAC.CTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                                <  1:5051291/101‑10 (MQ=2)
                                                        ccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGc                                              >  2:3069464/1‑101 (MQ=255)
                                                         cccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCt                                             >  2:2124197/1‑101 (MQ=255)
                                                           cgcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                           <  2:4937605/101‑1 (MQ=255)
                                                            gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                          <  1:5614687/101‑1 (MQ=255)
                                                                gggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgacg                                      <  1:1835291/101‑1 (MQ=255)
                                                                   cGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCt                                   >  2:1022526/1‑101 (MQ=255)
                                                                    gTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                                  >  2:2318996/1‑101 (MQ=255)
                                                                    gTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                                  >  2:660806/1‑101 (MQ=255)
                                                                     tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                 >  1:3544010/1‑101 (MQ=255)
                                                                     tGGATCGAAACTGCTTGC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                 <  1:2035679/101‑1 (MQ=255)
                                                                      ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                                >  1:541826/1‑101 (MQ=255)
                                                                      ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                                >  1:685797/1‑101 (MQ=255)
                                                                      ggATCGAAACTGCTTTC.GTGGGACTGACCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                                <  2:3675600/101‑1 (MQ=255)
                                                                       gATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                               <  2:779781/101‑1 (MQ=255)
                                                                        aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                              >  2:5795011/1‑101 (MQ=255)
                                                                        aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                              <  1:3485836/101‑1 (MQ=255)
                                                                          cGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt                            <  1:1713346/101‑1 (MQ=255)
                                                                           gAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                           >  1:567602/1‑101 (MQ=255)
                                                                            aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                          >  2:1086580/1‑101 (MQ=255)
                                                                            aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                          <  1:1518244/101‑1 (MQ=255)
                                                                             aaCTGCTTTC.GTGGGACTGGCCAGCCGCACGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTc                         <  1:5308965/101‑1 (MQ=255)
                                                                                tGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCggg                      >  2:4065245/1‑101 (MQ=255)
                                                                                tGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCggg                      <  1:2893667/101‑1 (MQ=255)
                                                                                tGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCggg                      <  1:2192012/101‑1 (MQ=255)
                                                                                 gCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt                     >  2:4006075/1‑101 (MQ=255)
                                                                                  cTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGAGTc                    <  1:2124314/101‑1 (MQ=255)
                                                                                   tttC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCg                   >  2:3767707/1‑101 (MQ=255)
                                                                                   tttC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCg                   >  1:5474068/1‑101 (MQ=255)
                                                                                   tttC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCg                   <  2:1763592/101‑1 (MQ=255)
                                                                                   tttC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCCTCGCTTACGACGCCTATCTGATTTCGGGTCg                   <  1:5125710/101‑1 (MQ=255)
                                                                                   ttgc.GTGGGACTGGCCAGCCTCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTGCGGGTCg                   <  1:3825923/98‑1 (MQ=255)
                                                                                   tctgcttGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTc                    >  2:2517207/7‑101 (MQ=255)
                                                                                      c.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc                <  2:3360183/101‑1 (MQ=255)
                                                                                          gggACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccc             >  2:449023/1‑101 (MQ=255)
                                                                                          gggACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGCCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccc             >  2:4707502/1‑101 (MQ=255)
                                                                                           ggACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccccc            >  1:81525/1‑101 (MQ=255)
                                                                                            gccTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccccc           <  2:1496185/99‑1 (MQ=255)
                                                                                              cTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgc         <  1:4921709/101‑1 (MQ=255)
                                                                                               tGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgcg        >  2:2560599/1‑101 (MQ=255)
                                                                                               tGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgcg        >  2:3889131/1‑101 (MQ=255)
                                                                                               tGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgcg        >  2:1166860/1‑101 (MQ=255)
                                                                                                ggCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCgg       >  1:3158435/1‑101 (MQ=255)
                                                                                                 gCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggg      <  1:2653785/101‑1 (MQ=255)
                                                                                                 gCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggg      <  2:5014946/101‑1 (MQ=255)
                                                                                                   cAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggggg    <  2:5429146/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
TGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCG.CCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCG  >  NC_014034/1396459‑1396659

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG