Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,570 C→T 13.7% P151L (CCA→CTA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5700C→T13.7% 116.3 ‑1.4 ‑0.2321P151L (CCA→CTA) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (18/26):  ref base (141/136):  total (159/162)
Fisher's exact test strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.13e-01

Only 100 of 349 total aligned reads displayed.

GGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1396470‑1396670
                                                                                                     |                                                                                                    
ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGcc                                                                                                      <  2:4102540/101‑1 (MQ=255)
ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGcc                                                                                                      >  1:1553928/1‑101 (MQ=255)
 gACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCa                                                                                                     <  2:357357/101‑1 (MQ=255)
 gACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCa                                                                                                     >  2:2531954/1‑101 (MQ=255)
   ccTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAg                                                                                                   <  2:4817231/101‑1 (MQ=255)
    cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGt                                                                                                  >  1:5339070/1‑101 (MQ=255)
     tGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTc                                                                                                 <  1:2280801/101‑1 (MQ=255)
      gAAATTCCGCGGGATCCCCGGCGGCGAGCACCTCGCTCCCCCCGCGGTGGCGTAGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCg                                                                                                <  1:666932/101‑1 (MQ=38)
      gAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCg                                                                                                >  1:1333043/1‑101 (MQ=255)
           tCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTccc                                                                                           <  1:395038/101‑1 (MQ=255)
           tCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTccc                                                                                           <  1:286862/101‑1 (MQ=255)
            ccGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTGCTTTC.GTGGGACTGGCCCGCCGCCCGCGCCAAGTCGCTcccc                                                                                          >  2:4522751/1‑101 (MQ=255)
              gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc                                                                                        >  1:1037161/1‑101 (MQ=255)
                gggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgc                                                                                      >  2:1835181/1‑101 (MQ=255)
                gggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCCCCCACcgc                                                                                      >  2:1025241/1‑101 (MQ=255)
                 ggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgcg                                                                                     >  1:5015227/1‑101 (MQ=255)
                   aaaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCggg                                                                                   >  1:1107208/1‑101 (MQ=255)
                    agcgcgGCGGCGAGCACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgggg                                                                                  <  1:2011981/99‑1 (MQ=255)
                      cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc                                                                                <  1:4424071/101‑1 (MQ=255)
                       gcggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCg                                                                               >  1:3034521/1‑101 (MQ=255)
                         ggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTg                                                                             <  2:337063/101‑1 (MQ=255)
                           cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGGCTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                           <  1:2930425/101‑1 (MQ=255)
                            tgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGa                                                                           >  1:211075/10‑101 (MQ=2)
                            ggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                          <  2:2906715/101‑1 (MQ=255)
                            ggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                          <  2:509823/101‑1 (MQ=255)
                            ggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                          >  2:5738638/1‑101 (MQ=255)
                             gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                         <  1:4238723/101‑1 (MQ=255)
                             gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                         <  1:4603931/101‑1 (MQ=255)
                              ggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATc                                                                         <  1:1435881/94‑1 (MQ=2)
                              cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                        >  2:4595277/1‑101 (MQ=255)
                              cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                        <  2:203886/101‑1 (MQ=255)
                                aGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                      <  1:1964826/101‑1 (MQ=255)
                                  ggaggcGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaaa                                                                     <  2:1755427/96‑1 (MQ=2)
                                  cgcGTCGCTCGCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaaa                                                                     <  2:4313641/99‑1 (MQ=2)
                                  caggTCACTCCCGCCGCGGGGGGGGGAATCGAAACTGATTAC.GTGGGCCTGGCCACCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                    <  2:4862953/98‑1 (MQ=21)
                                   gagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAAc                                                                    <  1:2667555/99‑1 (MQ=2)
                                   aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                                   <  2:1463288/101‑1 (MQ=255)
                                   aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                                   <  2:3471147/101‑1 (MQ=255)
                                    cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                                                  >  1:5312097/1‑101 (MQ=255)
                                    agTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                                   >  2:1637317/2‑100 (MQ=2)
                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgt                                                                 <  1:2439447/101‑4 (MQ=2)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                                               <  1:2964067/101‑1 (MQ=255)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                                                >  1:4097479/1‑97 (MQ=2)
                                        gccccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                                              >  1:761690/4‑101 (MQ=255)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgc                                                               >  1:941821/1‑96 (MQ=2)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgc                                                               >  2:694498/1‑96 (MQ=2)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTCGATCGAAActgtgc                                                               >  1:5099152/1‑96 (MQ=2)
                                         cTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgcc                                                              <  1:2145353/101‑7 (MQ=2)
                                           ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                            <  1:3640680/101‑9 (MQ=2)
                                             ccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggtt                                                          <  2:3333292/101‑11 (MQ=2)
                                             ccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggtt                                                          >  2:4977551/1‑91 (MQ=2)
                                              cccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCt                                                        <  2:5587915/101‑1 (MQ=255)
                                              cccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCt                                                        >  2:2124197/1‑101 (MQ=255)
                                                cgcgGTGGCGGGGATCGAAACTGCTTTC.GTGGGACTGGCCGGCCGCGCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                                      <  2:2987841/101‑1 (MQ=38)
                                                cgcgGGGTCGTGGATCGAAACTGCTTTC.GGGGGACTGGCCAGTAGCCTGCGACAAGCCGCTCCCCCCGCGGGCGCGTGGGTCGAAACGATTTCGCCGCTTa                                                      >  2:2563605/1‑101 (MQ=25)
                                                cgcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                                      <  2:4937605/101‑1 (MQ=255)
                                                 gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                                     >  1:5146712/1‑101 (MQ=255)
                                                    ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCCGGGGCGTGGATCGAAACGATTTCGTCGCTTccgac                                                  >  2:5299022/1‑101 (MQ=255)
                                                     gggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgacg                                                 <  1:1835291/101‑1 (MQ=255)
                                                      ggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGGCGCTTacgaccc                                                >  1:4279599/1‑99 (MQ=255)
                                                         gTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                                             >  2:2318996/1‑101 (MQ=255)
                                                           ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                                           >  1:5271151/1‑101 (MQ=255)
                                                           ggATCGAAACTGCTTTC.GTGGGACTGACCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                                           <  2:3675600/101‑1 (MQ=255)
                                                             aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                                         >  2:3929957/1‑101 (MQ=255)
                                                             aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                                         >  2:5795011/1‑101 (MQ=255)
                                                             aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                                         <  1:3485836/101‑1 (MQ=255)
                                                               cGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt                                       <  1:1713346/101‑1 (MQ=255)
                                                                gAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                                      >  1:567602/1‑101 (MQ=255)
                                                                 aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                                     >  2:2733531/1‑101 (MQ=255)
                                                                 aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                                     >  2:1086580/1‑101 (MQ=255)
                                                                  accTGCTTTC.TGGGGCCTGGCCGGCCGCCCGCTCCAAGTCTCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTc                                    <  2:745778/99‑1 (MQ=37)
                                                                  aaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTc                                    <  2:3767216/101‑1 (MQ=255)
                                                                     tGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCggg                                 >  1:2410256/1‑101 (MQ=255)
                                                                      gCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt                                <  2:2952017/101‑1 (MQ=255)
                                                                        tttC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCg                              >  1:5474068/1‑101 (MQ=255)
                                                                        tttC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCg                              <  2:4014739/101‑1 (MQ=255)
                                                                              tGGGACTGGCCAGCCACCCGCTGCTAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccc                         <  2:5489613/101‑1 (MQ=38)
                                                                               gggACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccc                        <  2:4473153/101‑1 (MQ=255)
                                                                               gggACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGCCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccc                        >  2:4707502/1‑101 (MQ=255)
                                                                                ggACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccccc                       <  2:81612/101‑1 (MQ=255)
                                                                                ggACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccccc                       >  1:81525/1‑101 (MQ=255)
                                                                                 gACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccccc                      <  2:3233686/101‑1 (MQ=255)
                                                                                    tGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgcg                   >  2:4558728/1‑101 (MQ=255)
                                                                                    tGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgcg                   >  1:2545107/1‑101 (MQ=255)
                                                                                    tGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgcg                   >  2:1166860/1‑101 (MQ=255)
                                                                                     ggCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCgg                  >  1:3158435/1‑101 (MQ=255)
                                                                                       ccAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCgggg                <  1:5582103/101‑1 (MQ=255)
                                                                                       ccAGCCGCCCGCGCCAAGTCCCTCCCCCCGCGGGGCCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCgggg                <  1:378901/101‑1 (MQ=255)
                                                                                        cAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggggg               >  1:1465314/1‑101 (MQ=255)
                                                                                        cAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggggg               <  2:3087489/101‑1 (MQ=255)
                                                                                         aGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCCCGGGGGc              >  1:4960448/1‑101 (MQ=255)
                                                                                          gccgccCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCg             <  2:2430699/101‑1 (MQ=255)
                                                                                               ccgcGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGAt        >  1:1872546/1‑101 (MQ=255)
                                                                                                cgggcCAAGTCGGTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGGCGCTCCCCCCGCGGGGGCGTGGATc       <  2:1295824/98‑1 (MQ=255)
                                                                                                 gcgcCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCg      <  2:3543793/101‑1 (MQ=255)
                                                                                                 gcgcCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCg      <  2:3843034/101‑1 (MQ=255)
                                                                                                  cgcCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCTCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGa     <  2:2842556/101‑1 (MQ=255)
                                                                                                   gcCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa    <  1:218418/101‑1 (MQ=255)
                                                                                                    ccAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa   <  1:4902460/101‑1 (MQ=255)
                                                                                                    ccAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa   >  2:852487/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
GGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1396470‑1396670

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG