BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,397,008	C→T	15.5%	S297L (TCG→TTG)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,397,008	0	C→T	15.5%	146.3 ‑1.2 ‑0.0	341	S297L (TCG→TTG)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (24/29): ref base (153/135): total (177/164)
Fisher's exact test strand distribution p-value = 3.00e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.59e-01

Only 100 of 375 total aligned reads displayed.


TCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCC  >  NC_014034/1396908‑1397108


  cccccGCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCtcgc                                                                                                         >  2:2087085/1‑100 (MQ=255)


  cccccGCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCACTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCtcg.t                                                                                                        <  1:1988384/101‑1 (MQ=255)


     ccGCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTcg.tcgt                                                                                                     <  2:3178568/101‑1 (MQ=255)


     ccGCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGAATCGAAAC.ATCAGCTcg.tcgt                                                                                                     <  2:2907424/101‑1 (MQ=255)


        cgGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTgg                                                                                                  >  2:4348316/1‑101 (MQ=255)


         gggggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGt                                                                                                 >  2:579705/1‑101 (MQ=255)


             gCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGATTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGc                                                                                             >  2:5228674/1‑101 (MQ=255)


              cGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGcc                                                                                            >  2:1250221/1‑101 (MQ=255)


               gTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCg                                                                                           >  1:1678737/1‑101 (MQ=255)


                 ggATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGACAGCCGGa                                                                                         <  2:1857742/101‑1 (MQ=255)


                  gATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATGGAAAC.ATCAACTCG.TCGTTGGTCAGCCGGAc                                                                                        >  1:3614762/1‑101 (MQ=255)


                  gATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAc                                                                                        <  1:2316727/101‑1 (MQ=255)


                       aaaCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGt                                                                                  >  2:3612103/1‑101 (MQ=255)


                        aaCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTc                                                                                 <  2:4751067/101‑1 (MQ=255)


                        aaCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTc                                                                                 <  1:2911494/101‑1 (MQ=255)


                         aCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTcc                                                                                >  1:4516402/1‑101 (MQ=255)


                         aCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTcc                                                                                >  2:2717481/1‑101 (MQ=255)


                           aGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...Gt                                                                           <  2:2402558/101‑1 (MQ=255)


                                   cgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTcccc                                                                   >  1:2456491/1‑101 (MQ=255)


                                       cGCATCGGCTTCCGCCTGCGTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCcgc                                                               <  1:674880/101‑1 (MQ=255)


                                        gCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCcgcg                                                              >  1:2222464/1‑101 (MQ=255)


                                        gCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GACGCTCCCCCcgtg                                                              >  1:4979035/1‑99 (MQ=255)


                                          aTCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCggg                                                            >  2:3351447/1‑101 (MQ=255)


                                             ggCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGc                                                         <  1:4768362/101‑1 (MQ=255)


                                               cTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCgg                                                       >  2:605655/1‑100 (MQ=255)


                                                ttCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                      >  2:4410464/1‑101 (MQ=255)


                                                ttCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                      >  1:1041593/1‑101 (MQ=255)


                                                  ggatcccgccggTCGCTCCCCGCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTg                                                      >  1:4267715/11‑101 (MQ=2)


                                                  ccGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGa                                                    <  1:3798738/101‑1 (MQ=255)


                                                  ccGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGa                                                    <  1:5213436/101‑1 (MQ=255)


                                                   gatcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                     <  1:214744/92‑1 (MQ=2)


                                                   cGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGAt                                                   >  2:3504379/1‑101 (MQ=255)


                                                    gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                  <  1:1250304/101‑1 (MQ=255)


                                                    gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                  <  2:1658284/101‑1 (MQ=255)


                                                    gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                  <  1:1813167/101‑1 (MQ=255)


                                                    gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                  <  1:4610672/101‑1 (MQ=255)


                                                    gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                  >  2:4005709/1‑101 (MQ=255)


                                                     tcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGAt                                                   <  1:2983761/94‑1 (MQ=2)


                                                     ccagcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                   >  2:832349/9‑101 (MQ=2)


                                                     ccTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 <  2:2843516/101‑1 (MQ=255)


                                                      cccgccggTCGCTGTCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGCCCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                  <  2:3856163/95‑1 (MQ=2)


                                                       tGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                               >  2:21799/1‑101 (MQ=255)


                                                        cgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                <  1:1132375/97‑1 (MQ=2)


                                                         cGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAAc                                             >  2:2260207/1‑101 (MQ=255)


                                                          ggggTCGCGCCCCCCGCGGGGGCGTGGATC.AAAC.CCCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                              <  2:1748998/99‑1 (MQ=2)


                                                          ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                              >  1:3446193/4‑101 (MQ=0)


                                                          gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                            <  2:5483654/101‑1 (MQ=255)


                                                          gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                            <  1:3269946/101‑1 (MQ=255)


                                                          gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                            <  2:1639463/101‑1 (MQ=255)


                                                          gTGGTCGCTCCCCCCGCGGGGGCGTGG.TCGAAACAATCGGTTCG.CGGTTGTTAGGCGGGAG.GAGGTCC...GTCCCTCCCCCCCCGGGGGGGGGGTTCGAACCt                                            >  1:3179087/1‑101 (MQ=9)


                                                           cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                             <  1:1567538/100‑1 (MQ=2)


                                                           cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                             <  1:1858969/100‑1 (MQ=2)


                                                            ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTNGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                            >  1:1749097/1‑100 (MQ=2)


                                                            cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaa                                            >  2:1081893/2‑99 (MQ=2)


                                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgg                                           >  2:1363450/1‑99 (MQ=2)


                                                               cccTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGACGt                                       <  1:4454549/99‑1 (MQ=255)


                                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcg                                         >  1:3560212/1‑100 (MQ=2)


                                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGt                                       <  1:5065379/101‑1 (MQ=255)


                                                                gcgccccccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTc                                      <  1:5269020/98‑1 (MQ=255)


                                                                gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTc                                      <  2:2504230/101‑1 (MQ=255)


                                                                  tCCCCCCGCGGGGGCGTTGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                      <  2:5146270/101‑5 (MQ=2)


                                                                  tCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                                    >  2:126490/1‑101 (MQ=255)


                                                                  cccccccGCGGTGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCCCC...GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                      <  2:5703996/100‑5 (MQ=2)


                                                                  cccccccGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                      <  2:1595560/100‑5 (MQ=2)


                                                                  cccccccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                                    <  2:4150303/100‑1 (MQ=255)


                                                                   ccccccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTg                                   <  2:1996031/101‑1 (MQ=255)


                                                                   ccccccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTg                                   >  1:4116965/1‑101 (MQ=255)


                                                                      cccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGt                                <  2:5031921/101‑1 (MQ=255)


                                                                      cccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGt                                <  2:4571202/101‑1 (MQ=255)


                                                                       ccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTg                               <  1:1444651/101‑1 (MQ=255)


                                                                       ccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTg                               <  1:1320834/101‑1 (MQ=255)


                                                                        cgcgGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtagatctcg                                <  1:2740762/101‑11 (MQ=0)


                                                                        cgcgGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGa                              >  2:5307689/1‑101 (MQ=255)


                                                                           gggggCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                           <  1:4348582/101‑1 (MQ=255)


                                                                           gggggCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                           <  1:417601/101‑1 (MQ=255)


                                                                           gggggCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                           >  1:3361209/1‑101 (MQ=255)


                                                                            ggggCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATggg                          <  1:1609888/101‑1 (MQ=255)


                                                                              ggCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgc                        <  1:605713/101‑1 (MQ=255)


                                                                               gCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcg                       >  1:1000163/1‑101 (MQ=255)


                                                                                  tGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGa                    >  1:5564240/1‑101 (MQ=255)


                                                                                   ggATCGAAAC.ATCAGCTCG.TCGTTGGTTAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGGGATGGGGCGCGAg                   >  1:5391774/1‑101 (MQ=255)


                                                                                   ggATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAg                   >  2:69822/1‑101 (MQ=255)


                                                                                    gATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGTGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                  <  2:4978754/101‑1 (MQ=255)


                                                                                      tCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcac                <  1:1864331/101‑1 (MQ=255)


                                                                                      tCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcac                <  2:1041518/101‑1 (MQ=255)


                                                                                      tCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcac                <  1:3985068/101‑1 (MQ=255)


                                                                                        gAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt              <  2:4516130/101‑1 (MQ=255)


                                                                                        gAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt              <  2:4286966/101‑1 (MQ=255)


                                                                                         aaaC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg             >  1:1360970/1‑101 (MQ=255)


                                                                                          aaC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgt            <  2:3284342/101‑1 (MQ=255)


                                                                                          aaC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGGGATGGGGCGCGAGCACATgt            >  2:4242268/1‑101 (MQ=255)


                                                                                               tCAGCTCG.TCGTTTGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcgtc        >  2:8891/1‑101 (MQ=255)


                                                                                               tCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcgtc        <  1:5723666/101‑1 (MQ=255)


                                                                                                cAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATctcgtcc       >  2:5620404/1‑100 (MQ=255)


                                                                                                cAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGtcgtcg       <  1:1922372/101‑1 (MQ=255)


                                                                                                 aGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGc      >  2:5080520/1‑101 (MQ=255)


                                                                                                  gCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGGACATGTCGTCGCt     >  1:4860515/1‑101 (MQ=255)


                                                                                                  gCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCt     <  1:2606820/101‑1 (MQ=255)


                                                                                                  gCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCt     <  2:4852460/101‑1 (MQ=255)


                                                                                                   cTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGAGCGAGCACATGTCGTCGCTc    >  2:375459/1‑101 (MQ=255)


TCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCC  >  NC_014034/1396908‑1397108

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG