Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,027 G→C 16.5% T303T (ACG→ACC RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,0270G→C16.5% 129.6 ‑0.3 ‑0.0359T303T (ACG→ACCRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (24/29):  ref base (134/146):  total (180/179)
Fisher's exact test strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 378 total aligned reads displayed.

ATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1396927‑1397127
                                                                                                       |                                                                                                       
aTCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.g                                                                                                         >  1:430438/1‑101 (MQ=255)
  cGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GAt                                                                                                       >  1:2833236/1‑101 (MQ=255)
    aaaCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGt                                                                                                     >  1:1460664/1‑101 (MQ=255)
     aaCAGCCTGCGCGGCCGCATCGCCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTc                                                                                                    <  2:3793627/101‑1 (MQ=255)
      aCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTcc                                                                                                   >  1:4516402/1‑101 (MQ=255)
       cAGCCTGCGCGGCCGCATCAGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...g                                                                                               <  1:3265856/101‑1 (MQ=255)
        aGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...Gt                                                                                              >  2:3899242/1‑101 (MQ=255)
         gCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTc                                                                                             <  2:3691992/101‑1 (MQ=255)
            tGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCt                                                                                          <  1:4454814/101‑1 (MQ=255)
             gcgcGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTc                                                                                         <  2:216078/101‑1 (MQ=255)
             gcgcGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTc                                                                                         >  1:4096206/1‑101 (MQ=255)
             gcgcGGCCGCATCGGCTCCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTc                                                                                         <  2:5194856/101‑1 (MQ=255)
               gcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTccc                                                                                       >  2:2329306/1‑101 (MQ=255)
                cgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTcccc                                                                                      >  1:2456491/1‑101 (MQ=255)
                 ggCCGCATCGGCTTCCGCCTGCGTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTccccc                                                                                     <  1:4135302/101‑1 (MQ=255)
                         cGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCggggg                                                                             >  2:5725811/1‑101 (MQ=255)
                          ggCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCAAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGc                                                                            <  2:4088154/101‑1 (MQ=255)
                           gCTTCCGCCTGCGTGGTCGCACCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCg                                                                           <  2:5687584/101‑1 (MQ=255)
                             ttCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                                         >  2:4410464/1‑101 (MQ=255)
                               ccGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGa                                                                       <  1:3798738/101‑1 (MQ=255)
                                gatcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                                        <  1:214744/92‑1 (MQ=2)
                                 gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  1:1250304/101‑1 (MQ=255)
                                 gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     >  1:5736583/1‑101 (MQ=255)
                                 gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  1:4610672/101‑1 (MQ=255)
                                 gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  1:1813167/101‑1 (MQ=255)
                                 atcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGa                                                                       <  2:3364082/93‑1 (MQ=2)
                                  ccagcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                      >  1:2946532/9‑101 (MQ=2)
                                   cccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  2:1726980/95‑1 (MQ=2)
                                   cccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  1:5105474/95‑1 (MQ=2)
                                     cgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                   >  2:5242643/5‑101 (MQ=2)
                                      cGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                <  1:50238/101‑1 (MQ=255)
                                       ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 >  1:3446193/4‑101 (MQ=0)
                                       gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                               <  2:1639463/101‑1 (MQ=255)
                                       gTGGTCGCTCCCCCCGCGGGGGCGTGG.TCGAAACAATCGGTTCG.CGGTTGTTAGGCGGGAG.GAGGTCC...GTCCCTCCCCCCCCGGGGGGGGGGTTCGAACCt                                                               >  1:3179087/1‑101 (MQ=9)
                                        cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                <  1:1858969/100‑1 (MQ=2)
                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGc                                                             >  1:2042340/1‑101 (MQ=2)
                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGc                                                             >  2:5075782/1‑101 (MQ=2)
                                            ccccccccccGCGGTGGCGTTGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGt                                                          <  2:793454/97‑1 (MQ=255)
                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcg                                                            >  1:5202010/1‑100 (MQ=2)
                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcg                                                            >  1:3560212/1‑100 (MQ=2)
                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGt                                                          >  2:2757552/1‑101 (MQ=255)
                                             ggaagccccGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgt                                                           >  2:292523/6‑99 (MQ=2)
                                             gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTc                                                         >  1:1602827/1‑101 (MQ=255)
                                             gCTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactg                                                           >  1:5620687/1‑96 (MQ=2)
                                              cTCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactga                                                          >  1:4361271/1‑95 (MQ=2)
                                               tCCCCCCGCGGGGGCGTTGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                                         <  2:5146270/101‑5 (MQ=2)
                                               tCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                                         >  2:59120/1‑97 (MQ=2)
                                               tCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                                         >  2:2044395/1‑97 (MQ=2)
                                               tCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtaa                                                         >  2:259648/1‑97 (MQ=2)
                                               tCCCCCCGCGGGGGCGTGGATCGAAAC.ATAAGCTCG.TAGTTGGTAAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                                                       >  1:5364597/1‑101 (MQ=255)
                                               tCCCCCCGCGGGGGCGTGGATCGAAAC..CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactgac                                                         >  2:4891758/1‑94 (MQ=2)
                                                ccccccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTg                                                      >  1:1052064/1‑101 (MQ=255)
                                                 cccccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGa                                                     <  1:1400385/101‑1 (MQ=255)
                                                   cccGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGt                                                   <  2:4571202/101‑1 (MQ=255)
                                                     cgcgGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGa                                                 >  1:1338911/1‑101 (MQ=255)
                                                     cgcgGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGa                                                 <  2:1956222/101‑1 (MQ=255)
                                                     cgcgGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGa                                                 <  2:3554477/101‑1 (MQ=255)
                                                        gggggCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                                              >  2:1581755/1‑101 (MQ=255)
                                                        gggggCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCA...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                                              >  2:243757/1‑101 (MQ=255)
                                                            gCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcg                                          >  1:1000163/1‑101 (MQ=255)
                                                              gTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcg                                        >  2:2233359/1‑101 (MQ=255)
                                                               tGGATCGAAAC.ATCAGCTCG.TCGCTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACTGCCGTCTTGAGTGATGGGGcgcgg                                       >  2:1678559/1‑100 (MQ=255)
                                                                ggATCGAAAC.ATCAGCTNG.TCGTGGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAg                                      <  2:1259883/101‑1 (MQ=255)
                                                                ggATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGac                                      >  2:4787265/1‑100 (MQ=255)
                                                                ggATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAg                                      >  2:4310755/1‑101 (MQ=255)
                                                                 gATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGTGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                                     <  2:4978754/101‑1 (MQ=255)
                                                                 gATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                                     <  1:4712274/101‑1 (MQ=255)
                                                                 gATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                                     <  2:179504/101‑1 (MQ=255)
                                                                     gAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt                                 <  2:4516130/101‑1 (MQ=255)
                                                                     gAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt                                 >  1:5267650/1‑101 (MQ=255)
                                                                     gAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt                                 <  1:4902285/101‑1 (MQ=255)
                                                                      aaaC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACCTg                                >  1:5524945/1‑101 (MQ=255)
                                                                       aaC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGGGGGAGGACATgt                               >  1:1020729/1‑101 (MQ=255)
                                                                       aaC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGGGATGGGGCGCGAGCACATgt                               >  2:4242268/1‑101 (MQ=255)
                                                                        aC.AGCAGCTCG.TCGTTGGGCAGCCGCAC.GATGTCC...GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtc                              <  2:1070853/101‑1 (MQ=38)
                                                                         c.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcg                             >  2:3262754/1‑101 (MQ=255)
                                                                         c.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcg                             >  2:3333165/1‑101 (MQ=255)
                                                                             cAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGtcgtcg                          <  1:1922372/101‑1 (MQ=255)
                                                                              aGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGc                         <  1:3351652/101‑1 (MQ=255)
                                                                               gCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCt                        <  1:666610/101‑1 (MQ=255)
                                                                               gCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCt                        <  1:2606820/101‑1 (MQ=255)
                                                                                cTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGACTGATGGGGCGCGAGCACATGTCGCCGCTc                       >  1:1876441/1‑101 (MQ=255)
                                                                                 tcg.tcgTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGGGAGCACATGTCGTCGCTcc                      >  2:5445910/1‑101 (MQ=255)
                                                                                      cgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc                  >  1:2696443/1‑101 (MQ=255)
                                                                                      cgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc                  <  1:2355256/101‑1 (MQ=255)
                                                                                        ttGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcgc                <  1:4808568/101‑1 (MQ=255)
                                                                                            tCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCgggg            <  1:1721505/101‑1 (MQ=255)
                                                                                            tCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCgggg            <  2:1000088/101‑1 (MQ=255)
                                                                                            tCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCgggg            <  1:762990/101‑1 (MQ=255)
                                                                                             cAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCggggg           >  2:653898/1‑101 (MQ=255)
                                                                                              aGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGc          <  1:3678018/101‑1 (MQ=255)
                                                                                               gCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCg         <  1:4217567/101‑1 (MQ=255)
                                                                                                 cGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGATCCCCCCGCGGGGGCGTg       >  2:889879/1‑101 (MQ=255)
                                                                                                  ggAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTgg      >  2:1162465/1‑101 (MQ=255)
                                                                                                    aC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGAt    <  1:2260333/101‑1 (MQ=255)
                                                                                                    aC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGAt    <  2:2833075/101‑1 (MQ=255)
                                                                                                     c.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATc   <  1:397562/101‑1 (MQ=255)
                                                                                                       gATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCg  <  2:5568394/101‑1 (MQ=255)
                                                                                                       gATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCg  <  2:1308663/101‑1 (MQ=255)
                                                                                                       gATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGGGGATCg  >  1:3371070/1‑101 (MQ=255)
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ATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1396927‑1397127

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG