Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,135 C→A 4.2% S339S (TCC→TAA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,1350C→A 4.2% 18.3 ‑2.8 ‑0.0264S339S (TCC→TCA) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/9):  ref base (129/124):  total (131/133)
Fisher's exact test strand distribution p-value = 6.00e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.92e-01

Only 100 of 291 total aligned reads displayed.

TCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGT  >  NC_014034/1397035‑1397235
                                                                                                    |                                                                                                     
tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTcc                                                                                                       <  1:4005956/101‑1 (MQ=255)
 cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCa                                                                                                      >  1:5540180/1‑101 (MQ=255)
 cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCa                                                                                                      >  2:4268728/1‑101 (MQ=255)
 cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCCCGTCTTGAGTGATGGGGCGCGAGCCCATGTCGTCGCTCCCACCGCGGGGGGGTGGATCGAAACGTCCa                                                                                                      >  2:765998/1‑101 (MQ=38)
  gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAg                                                                                                     >  1:690242/1‑101 (MQ=255)
    tCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCt                                                                                                   <  1:3333370/101‑1 (MQ=255)
    tCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCt                                                                                                   >  1:1100904/1‑101 (MQ=255)
    tCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGTCCAGcc                                                                                                   >  2:3945204/1‑100 (MQ=255)
     ccccccGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTc                                                                                                  <  2:2696294/101‑1 (MQ=255)
     ccccccGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTc                                                                                                  >  1:4787098/1‑101 (MQ=255)
       ccccGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTcgc                                                                                                <  1:5043658/101‑1 (MQ=255)
        cccGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTcgcg                                                                                               <  1:2237822/101‑1 (MQ=255)
          cgcgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGcc                                                                                             <  2:4661378/101‑1 (MQ=255)
          cgcgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGcc                                                                                             <  1:705298/101‑1 (MQ=255)
            cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                                                                                           >  2:789303/1‑101 (MQ=255)
            cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                                                                                           >  1:840885/1‑101 (MQ=255)
              ggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                                                                                         <  2:2212354/101‑1 (MQ=255)
               gggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATg                                                                                        <  2:3696513/101‑1 (MQ=255)
                ggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGt                                                                                       >  1:5242182/1‑101 (MQ=255)
                ggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGt                                                                                       >  1:4446818/1‑101 (MQ=255)
                  cGTGGATCTAAACTGCCGTCTTTAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCt                                                                                     <  1:2757713/101‑1 (MQ=255)
                   gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt                                                                                    <  2:5148201/101‑1 (MQ=255)
                   gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt                                                                                    >  2:2433880/1‑101 (MQ=255)
                     ggATCGAAACGGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg                                                                                  <  2:1020654/101‑1 (MQ=255)
                       aTCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAc                                                                                >  2:380837/1‑101 (MQ=255)
                        tCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAcc                                                                               <  2:3596315/101‑1 (MQ=255)
                         cGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.g                                                                             <  2:695648/101‑1 (MQ=255)
                         cGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.g                                                                             <  1:1671966/101‑1 (MQ=255)
                          gAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.Gt                                                                            <  1:2432111/101‑1 (MQ=255)
                           aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTg                                                                           >  1:5775591/1‑101 (MQ=255)
                           aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGGCTTCGACC.GTg                                                                           >  2:2963111/1‑101 (MQ=255)
                              cTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTgcgc                                                                        <  2:3568065/101‑1 (MQ=255)
                               tGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGcc                                                                       <  1:243790/101‑1 (MQ=255)
                               tGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGcc                                                                       <  2:4786817/101‑1 (MQ=255)
                                      ttGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTc                                                                >  2:3757710/1‑101 (MQ=255)
                                       tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcc                                                               <  2:1876331/101‑1 (MQ=255)
                                       tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcc                                                               >  2:2190667/1‑101 (MQ=255)
                                         aGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccc                                                             >  1:2928425/1‑101 (MQ=255)
                                          gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccccc                                                            <  1:5017990/101‑1 (MQ=255)
                                           tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccccc                                                           >  1:3154092/1‑101 (MQ=255)
                                           tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAACTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccccc                                                           <  1:2042413/101‑1 (MQ=255)
                                            gATGGGGCGCGAGCACATGTCGACGCCGCCCCCGGGGGGGCGTGGATCGAAACGGCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                          <  2:1268944/101‑1 (MQ=38)
                                             aTGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcgc                                                         >  2:2786131/1‑101 (MQ=255)
                                               ggggCGCGAGCACATGTCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCgg                                                       <  1:5620696/101‑1 (MQ=255)
                                                gggCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCggg                                                      >  1:964139/1‑101 (MQ=255)
                                                   cgcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGc                                                   >  1:780723/1‑101 (MQ=255)
                                                   cgcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGc                                                   <  1:5179833/101‑1 (MQ=255)
                                                     cgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCACGCGGGGGCGt                                                 >  2:2382890/1‑101 (MQ=255)
                                                      gAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                >  1:4685616/1‑101 (MQ=255)
                                                       aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                               <  1:1785627/101‑1 (MQ=255)
                                                       aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                               >  2:344490/1‑101 (MQ=255)
                                                       aGCACATGTCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                               <  1:1968736/101‑1 (MQ=255)
                                                          acaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  2:1152893/101‑1 (MQ=255)
                                                           gctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  2:2830031/95‑1 (MQ=0)
                                                           caTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  1:2102726/1‑101 (MQ=255)
                                                            ctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          <  2:184872/96‑1 (MQ=2)
                                                            ctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          <  2:1851869/96‑1 (MQ=2)
                                                             tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  1:3885446/97‑1 (MQ=2)
                                                             tGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         >  1:5457366/1‑101 (MQ=255)
                                                              gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        >  1:265886/1‑101 (MQ=255)
                                                              gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        >  2:4990871/1‑101 (MQ=255)
                                                              gcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        >  2:405911/4‑101 (MQ=2)
                                                               tcgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       <  1:1389436/101‑1 (MQ=255)
                                                               tcggcgCTCCCCCCTCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCGCATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       <  2:3854730/101‑1 (MQ=255)
                                                                cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaag                                      <  1:4242528/101‑3 (MQ=255)
                                                                  tcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtg                                    <  2:4583927/101‑1 (MQ=255)
                                                                   cgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtgt                                   >  2:816497/1‑101 (MQ=255)
                                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTc                                  >  2:5798820/1‑101 (MQ=255)
                                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                                 <  1:3612331/101‑1 (MQ=255)
                                                                        cccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGcc                              >  1:4543791/1‑101 (MQ=255)
                                                                         ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                             <  2:5267360/101‑1 (MQ=255)
                                                                         ccccGCGGGGGCGTGGATCGAAACGTCCAGCTAGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGTTCGAAACGTGTCTGCCa                             <  2:5709281/101‑1 (MQ=255)
                                                                          cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCac                            >  1:3212384/1‑101 (MQ=255)
                                                                          cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCac                            <  2:2580727/101‑1 (MQ=255)
                                                                            cgcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacga                          >  1:2584201/1‑101 (MQ=255)
                                                                               gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGGCACGACGc                       >  2:507415/1‑101 (MQ=255)
                                                                               gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGc                       >  2:1563953/1‑101 (MQ=255)
                                                                               gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGc                       >  2:1970983/1‑101 (MQ=255)
                                                                                 gggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGccc                     <  1:5392722/101‑1 (MQ=255)
                                                                                 gggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGccc                     >  2:1832088/1‑101 (MQ=255)
                                                                                  ggCGTGGATCGAAACGTGCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCTCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCt                    >  2:5764287/1‑101 (MQ=255)
                                                                                  ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCt                    <  2:5563948/101‑1 (MQ=255)
                                                                                  ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCt                    <  1:4295234/101‑1 (MQ=255)
                                                                                   gCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTg                   >  1:1602351/1‑101 (MQ=255)
                                                                                     gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCa                 >  1:4853859/1‑101 (MQ=255)
                                                                                       ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAcc               >  2:142519/1‑101 (MQ=255)
                                                                                        gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCa              >  1:4909791/1‑101 (MQ=255)
                                                                                        gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCa              >  1:3845603/1‑101 (MQ=255)
                                                                                        gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCACGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCa              <  1:2383024/101‑1 (MQ=255)
                                                                                         aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCCCGACGCCCTGCACCAg             >  2:104493/1‑101 (MQ=255)
                                                                                         aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAg             <  2:1932465/101‑1 (MQ=255)
                                                                                           cGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.TTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGAt           <  2:5364307/101‑1 (MQ=255)
                                                                                            gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATc          <  2:4518032/101‑1 (MQ=255)
                                                                                            gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATc          >  1:1211689/1‑101 (MQ=255)
                                                                                            gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGACAGAAACGTGTCTGCCACGACGCACTGCACCCGaca          >  2:1689792/1‑99 (MQ=38)
                                                                                             aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATcc         <  2:3153903/101‑1 (MQ=255)
                                                                                                  tCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGTCTGCCACGGCGCCCCGCACCAGATCCGCGcc    >  2:5427405/1‑101 (MQ=255)
                                                                                                   ccAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCg   >  2:4619573/1‑101 (MQ=255)
                                                                                                    cAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGt  <  2:3297716/101‑1 (MQ=255)
                                                                                                    cAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGt  <  1:3570896/101‑1 (MQ=255)
                                                                                                    |                                                                                                     
TCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGT  >  NC_014034/1397035‑1397235

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG