Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,216 C→A 2.2% P366P (CCC→ACA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2160C→A 2.2% 5.0 ‑2.3 ‑0.0264P366P (CCC→CCARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/5):  ref base (139/119):  total (140/124)
Fisher's exact test strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.79e-01

Only 100 of 291 total aligned reads displayed.

GGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGG  >  NC_014034/1397116‑1397316
                                                                                                      |                                                                                                    
 ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCt                                                                                                     <  1:4295234/101‑1 (MQ=255)
  gCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCtc                                                                                                    >  2:1014547/1‑100 (MQ=255)
   cGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGc                                                                                                   >  2:5262754/1‑101 (MQ=255)
   cGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGc                                                                                                   >  1:550717/1‑101 (MQ=255)
    gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCa                                                                                                  >  1:4853859/1‑101 (MQ=255)
     tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCAc                                                                                                 >  1:1417566/1‑101 (MQ=255)
      ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCAcc                                                                                                >  2:142519/1‑101 (MQ=255)
      ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCAcc                                                                                                <  1:3817779/101‑1 (MQ=255)
       gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCa                                                                                               >  1:4909791/1‑101 (MQ=255)
        aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAg                                                                                              >  1:2328556/1‑101 (MQ=255)
          cGAAACGTCCAGCTCGCGCCACATGTCTTCTACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGAt                                                                                            <  1:2612577/101‑1 (MQ=255)
            aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATcc                                                                                          <  2:3153903/101‑1 (MQ=255)
            aaaCGTCCAGCTCGCCACACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATcc                                                                                          <  2:4548764/101‑1 (MQ=255)
             aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCcg                                                                                         <  2:1051989/101‑1 (MQ=255)
              aCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCcgc                                                                                        <  2:1360882/101‑1 (MQ=255)
               cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCcgcg                                                                                       >  2:3785867/1‑101 (MQ=255)
                 tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGcc                                                                                     <  2:5775296/101‑1 (MQ=255)
                  ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCg                                                                                    >  2:4619573/1‑101 (MQ=255)
                  ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCg                                                                                    <  2:3194425/101‑1 (MQ=255)
                     gCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCg                                                                                 >  1:2793831/1‑101 (MQ=255)
                     gCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCg                                                                                 >  2:5741348/1‑101 (MQ=255)
                       tCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCt                                                                               >  2:3450100/1‑101 (MQ=255)
                        cgcgCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTc                                                                              <  1:5282038/101‑1 (MQ=255)
                         gcgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTcc                                                                             <  2:3979627/101‑1 (MQ=255)
                         gcgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTcc                                                                             <  2:182194/101‑1 (MQ=255)
                          cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTccc                                                                            <  1:1293538/101‑1 (MQ=255)
                            ccccaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTccccc                                                                          <  2:1253076/98‑1 (MQ=255)
                            ccACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTccccc                                                                          >  1:3951528/1‑101 (MQ=255)
                              acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCcg                                                                        <  1:126510/101‑1 (MQ=255)
                                aTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCcgcg                                                                      >  2:4062058/1‑101 (MQ=255)
                                aTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGGGGGTCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGCCGCTCCCCCcgcg                                                                      >  1:7571/1‑101 (MQ=255)
                                  gTCTTCGGCCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggg                                                                    <  2:2807003/101‑1 (MQ=255)
                                   tCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCgggg                                                                   >  1:3409905/1‑101 (MQ=255)
                                   tCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCgggg                                                                   >  2:2270507/1‑101 (MQ=255)
                                   tCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCgggg                                                                   <  1:2087202/101‑1 (MQ=255)
                                    cTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggggg                                                                  >  1:1328427/1‑101 (MQ=255)
                                        gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                              <  1:4342729/101‑1 (MQ=255)
                                        gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                              <  2:5169394/101‑1 (MQ=255)
                                         gccGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                             <  1:2865246/100‑1 (MQ=255)
                                         gccGTGCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                             <  2:213247/100‑1 (MQ=255)
                                            gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  1:5080805/101‑1 (MQ=255)
                                             tGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         >  2:542216/1‑101 (MQ=255)
                                              gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        <  2:4272988/101‑1 (MQ=255)
                                              ctatcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGA.CCTGGTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  1:2658345/97‑1 (MQ=2)
                                                gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      >  1:1842094/1‑101 (MQ=255)
                                                 tcgccgcctcccccGCGGGGGCGTCGATCCAAGCGTGTCTGCCA.CGACG.CCCTGCACCAGAGCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                     <  2:5561198/92‑1 (MQ=25)
                                                 gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCA.ACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      <  2:4374305/100‑1 (MQ=2)
                                                 ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                     <  2:4116134/101‑1 (MQ=255)
                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                                  >  2:20135/1‑101 (MQ=255)
                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                               >  1:3323721/1‑101 (MQ=255)
                                                         ccccccGCGGGGGCGTGGATCGAACCGTGTCTGCCA.CGACC.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCa                                             <  1:5443703/101‑1 (MQ=255)
                                                         ccccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCa                                             <  2:185054/101‑1 (MQ=255)
                                                              cgcgGGGGCGTGGATCGAAACGTGTCTGCCA.CGTCG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgc                                        <  1:4992621/101‑1 (MQ=255)
                                                                cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                                      >  1:4734736/1‑101 (MQ=255)
                                                                   gggCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                                   <  1:5720687/101‑1 (MQ=255)
                                                                   gggCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                                   >  2:1920739/1‑101 (MQ=255)
                                                                    ggCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCGCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                                  >  1:386068/1‑101 (MQ=255)
                                                                    ggCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                                  >  2:831434/1‑101 (MQ=255)
                                                                      cGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                                >  1:890369/1‑101 (MQ=255)
                                                                      cGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                                <  2:5524653/101‑1 (MQ=255)
                                                                      cGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGACCGCGCGAACgggg                                >  1:1171763/1‑99 (MQ=255)
                                                                       gTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                               >  2:2187488/1‑101 (MQ=255)
                                                                       gTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                               <  2:2928258/101‑1 (MQ=255)
                                                                        tGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTc                              >  2:5029441/1‑101 (MQ=255)
                                                                         ggATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTAGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                             >  2:5008388/1‑101 (MQ=255)
                                                                           aTCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGc                           >  1:3689442/1‑101 (MQ=255)
                                                                            tCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcc                          >  1:5244605/1‑101 (MQ=255)
                                                                             cGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc                         <  1:3016944/101‑1 (MQ=255)
                                                                             cGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc                         >  1:5682347/1‑101 (MQ=255)
                                                                              gAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGACCGGCGGCAGcccc                        >  2:2659478/1‑101 (MQ=255)
                                                                              gAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                        >  2:3088320/1‑101 (MQ=255)
                                                                              gAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                        >  2:3325284/1‑101 (MQ=255)
                                                                              gAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                        >  2:4489875/1‑101 (MQ=255)
                                                                              gAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                        >  2:3945391/1‑101 (MQ=255)
                                                                                 aCGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgcg                     >  1:5797042/1‑101 (MQ=255)
                                                                                   gtgtCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                   >  1:4583628/1‑101 (MQ=255)
                                                                                    tgtCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc                  <  1:4424552/101‑1 (MQ=255)
                                                                                     gtCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                 >  2:3515338/1‑101 (MQ=255)
                                                                                       cTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc               <  2:3845361/101‑1 (MQ=255)
                                                                                       cTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc               <  1:4490147/101‑1 (MQ=255)
                                                                                        tGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTcc              >  1:1027131/1‑100 (MQ=255)
                                                                                         gCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc             >  1:2188442/1‑101 (MQ=255)
                                                                                           cA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTc           <  1:1920848/101‑1 (MQ=255)
                                                                                           cA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTc           <  1:20145/101‑1 (MQ=255)
                                                                                            a.cgacg.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcc          >  1:5131508/1‑101 (MQ=255)
                                                                                            a.cgacg.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcc          >  2:1198968/1‑101 (MQ=255)
                                                                                              cgacg.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccc         <  1:3523521/101‑1 (MQ=255)
                                                                                              cgacg.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccc         <  1:3245599/101‑1 (MQ=255)
                                                                                               gacg.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccc        <  1:1720649/101‑1 (MQ=255)
                                                                                                acg.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccccc       <  1:1014622/101‑1 (MQ=255)
                                                                                                 cg.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccccc      >  2:4803271/1‑101 (MQ=255)
                                                                                                 cg.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccccc      <  1:1549769/101‑1 (MQ=255)
                                                                                                  g.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg     <  2:1724190/101‑1 (MQ=255)
                                                                                                  g.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCCCGAAAGAAGCTCCCCCcg     >  2:5344343/1‑101 (MQ=37)
                                                                                                    cccTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcgc    >  2:5064199/1‑101 (MQ=255)
                                                                                                    cccTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcgc    <  2:238893/101‑1 (MQ=255)
                                                                                                     ccTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcgcg   >  1:2420866/1‑101 (MQ=255)
                                                                                                     ccTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcgcg   <  2:1526534/101‑1 (MQ=255)
                                                                                                     ccTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcgcg   <  2:1602257/101‑1 (MQ=255)
                                                                                                      cTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGCCGCTCCCCCCGCgg  >  2:3604598/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
GGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGG  >  NC_014034/1397116‑1397316

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG