Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,228 C→A 1.9% S370S (TCC→TCA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2280C→A 1.9% 4.3 ‑1.7 ‑0.4264S370S (TCC→TCARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (139/119):  total (140/124)
Fisher's exact test strand distribution p-value = 1.89e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.99e-01

Only 100 of 286 total aligned reads displayed.

AAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCG  >  NC_014034/1397128‑1397328
                                                                                                      |                                                                                                    
aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATcc                                                                                                      <  2:3153903/101‑1 (MQ=255)
   cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCcgcg                                                                                                   >  1:4437727/1‑101 (MQ=255)
   cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCcgcg                                                                                                   >  2:3785867/1‑101 (MQ=255)
      ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCg                                                                                                <  1:2749147/101‑1 (MQ=255)
      ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCg                                                                                                <  2:3194425/101‑1 (MQ=255)
      ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCg                                                                                                <  2:2079765/101‑1 (MQ=255)
      ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCg                                                                                                >  2:1549675/1‑101 (MQ=255)
         gCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCg                                                                                             >  2:5741348/1‑101 (MQ=255)
            cgcgCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTc                                                                                          <  1:3725027/101‑1 (MQ=255)
             gcgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTcc                                                                                         <  2:3979627/101‑1 (MQ=255)
             gcgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTcc                                                                                         <  2:182194/101‑1 (MQ=255)
             gcgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTcc                                                                                         >  2:3560924/1‑101 (MQ=255)
              cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTccc                                                                                        <  1:4787546/101‑1 (MQ=255)
              cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTccc                                                                                        <  1:1293538/101‑1 (MQ=255)
                ccACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTccccc                                                                                      >  1:3951528/1‑101 (MQ=255)
                  acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCcg                                                                                    <  1:126510/101‑1 (MQ=255)
                      gTCTTCGGCCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggg                                                                                <  2:2807003/101‑1 (MQ=255)
                       tCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCgggg                                                                               >  2:2270507/1‑101 (MQ=255)
                        cTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggggg                                                                              <  2:5241892/101‑1 (MQ=255)
                        cTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggggg                                                                              <  2:1467776/101‑1 (MQ=255)
                        cTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggggg                                                                              >  1:1328427/1‑101 (MQ=255)
                          tCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGCCG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCg                                                                            >  1:3454148/1‑101 (MQ=255)
                            gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                                          <  1:4342729/101‑1 (MQ=255)
                            gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                                          >  1:18867/1‑101 (MQ=255)
                               cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       >  2:4046397/1‑101 (MQ=255)
                               cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       >  2:4103890/1‑101 (MQ=255)
                               cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       <  2:4543519/101‑1 (MQ=255)
                                  ctatcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGA.CCTGGTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     <  1:2658345/97‑1 (MQ=2)
                                    gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  <  2:5035369/101‑1 (MQ=255)
                                     ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                 >  1:3013265/1‑101 (MQ=255)
                                     ccGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                 <  1:5032495/101‑1 (MQ=255)
                                      cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                                >  2:3432715/1‑100 (MQ=255)
                                      cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                >  1:1800718/1‑101 (MQ=255)
                                      cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCA.ACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                 <  1:1168617/101‑1 (MQ=2)
                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                               >  2:4418445/1‑101 (MQ=255)
                                           cTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                                           >  1:2348486/1‑101 (MQ=255)
                                           cTCCCCCCGCGGGGGCGTGGATCGAAACGTGGCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                                           >  1:5129663/1‑101 (MQ=255)
                                              cgcccGGGGGGGGGTGAATCGAAACGTGTCTGCCA.CGCCC.CCCTGCACCAGATCCCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                                                        <  2:1233854/99‑1 (MQ=25)
                                                 ccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcccc                                                     >  1:219753/1‑98 (MQ=255)
                                                  cgcgGGGGCGTGGATCGAAACGTGTCTGCCA.CGTCG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgc                                                    <  1:4992621/101‑1 (MQ=255)
                                                  cgcgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgc                                                    <  1:1951805/101‑1 (MQ=255)
                                                   gcgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgcg                                                   <  2:5539888/101‑1 (MQ=255)
                                                    cgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgcgc                                                  >  2:4594879/1‑100 (MQ=255)
                                                     gggggCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                                                 >  1:3554904/1‑101 (MQ=255)
                                                     gggggCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                                                 >  2:4348960/1‑101 (MQ=255)
                                                       gggCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                                               >  2:1920739/1‑101 (MQ=255)
                                                       gggCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                                               <  1:5720687/101‑1 (MQ=255)
                                                        ggCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                                              >  2:831434/1‑101 (MQ=255)
                                                        ggCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                                              >  1:2123283/1‑101 (MQ=255)
                                                           gTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                                           <  2:2928258/101‑1 (MQ=255)
                                                            tGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTc                                          >  2:464632/1‑101 (MQ=255)
                                                            gggATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTc                                          <  1:2580831/100‑1 (MQ=255)
                                                             ggATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTAGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                                         >  2:5008388/1‑101 (MQ=255)
                                                               aTCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGc                                       >  1:5253518/1‑101 (MQ=255)
                                                               aTCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGc                                       >  2:1394381/1‑101 (MQ=255)
                                                                 cGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc                                     >  1:5682347/1‑101 (MQ=255)
                                                                 cGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc                                     >  1:384344/1‑101 (MQ=255)
                                                                  gAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                                    >  2:3088320/1‑101 (MQ=255)
                                                                  gAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                                    >  2:3325284/1‑101 (MQ=255)
                                                                  gAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                                    <  1:5294969/101‑1 (MQ=255)
                                                                    aaCGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgc                                  >  1:3248233/1‑101 (MQ=255)
                                                                     acttgtCTGCCG.CGTCG.CCCTGCACCAGATCCGCGCCGTCGCATCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTTAGCCCcgcg                                 <  2:5778063/98‑1 (MQ=37)
                                                                     aCGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgcg                                 >  1:5797042/1‑101 (MQ=255)
                                                                       gtgtCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                               >  2:2132527/1‑101 (MQ=255)
                                                                       gtgtCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                               <  2:2624583/101‑1 (MQ=255)
                                                                         gtCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                             >  2:4209994/1‑101 (MQ=255)
                                                                          tCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGCACGGCGTGAGCCCCGCGCACGt                            >  2:1271837/1‑101 (MQ=255)
                                                                           cTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc                           <  1:1707013/101‑1 (MQ=255)
                                                                           cTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc                           <  1:4705405/101‑1 (MQ=255)
                                                                           cTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc                           <  2:3845361/101‑1 (MQ=255)
                                                                            tGCCG.CGACG.CCCTGCACCAGATCCGCGCCGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGGCAGCCCCGCGAACGTCg                          >  2:5557956/1‑101 (MQ=255)
                                                                            tGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTcc                          >  1:1027131/1‑100 (MQ=255)
                                                                            tGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg                          <  2:5129377/101‑1 (MQ=255)
                                                                             gCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc                         >  1:4281199/1‑101 (MQ=255)
                                                                               cA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTc                       <  1:20145/101‑1 (MQ=255)
                                                                                a.cgacg.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcc                      >  2:1198968/1‑101 (MQ=255)
                                                                                  cgacg.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccc                     <  1:2848501/101‑1 (MQ=255)
                                                                                    acg.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccccc                   <  1:1014622/101‑1 (MQ=255)
                                                                                      g.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg                 <  1:781381/101‑1 (MQ=255)
                                                                                      g.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg                 >  2:3503487/1‑101 (MQ=255)
                                                                                      g.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg                 <  2:1724190/101‑1 (MQ=255)
                                                                                      g.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCCCGAAAGAAGCTCCCCCcg                 >  2:5344343/1‑101 (MQ=37)
                                                                                        cccTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcgc                <  2:238893/101‑1 (MQ=255)
                                                                                          cTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCgg              >  1:5194971/1‑101 (MQ=255)
                                                                                          cTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCgg              >  2:219089/1‑101 (MQ=255)
                                                                                            gCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGa            <  2:1976878/101‑1 (MQ=255)
                                                                                              aCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCTGCGGGGGc          >  2:2713559/1‑101 (MQ=255)
                                                                                              aCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc          >  1:141572/1‑101 (MQ=255)
                                                                                              aCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc          >  1:3328207/1‑101 (MQ=255)
                                                                                               ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGCGCg         >  1:1475391/1‑101 (MQ=255)
                                                                                                cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt        >  1:5517509/1‑101 (MQ=255)
                                                                                                cAGACCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt        <  2:5410438/101‑1 (MQ=255)
                                                                                                 aGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGCACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg       >  2:3672472/1‑101 (MQ=255)
                                                                                                 aGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg       <  2:4793067/101‑1 (MQ=255)
                                                                                                  gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg      <  1:3176150/101‑1 (MQ=255)
                                                                                                   aTCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCCCGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTggg     >  1:833890/1‑100 (MQ=255)
                                                                                                   aTCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGa     >  1:973722/1‑101 (MQ=255)
                                                                                                     ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc   <  2:1064734/101‑1 (MQ=255)
                                                                                                     ccGCGCCGTCGCTCCCCCAGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGCACGTCGCTCACCCCGCGGGAGCGTGGAt  >  2:5483930/1‑100 (MQ=255)
                                                                                                      cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg  <  1:456753/101‑1 (MQ=255)
                                                                                                      |                                                                                                    
AAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACG.CCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCG  >  NC_014034/1397128‑1397328

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG