Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,552 C→G 11.5% L478L (CTC→GTG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5520C→G11.5% 71.9 ‑0.5 ‑0.0320L478L (CTC→CTGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/19):  ref base (130/135):  total (154/166)
Fisher's exact test strand distribution p-value = 5.80e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.07e-01

Only 100 of 353 total aligned reads displayed.

GCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1397452‑1397652
                                                                                                        |                                                                                                      
gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTc                                                                                                        <  1:2928391/101‑1 (MQ=255)
 cGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGATCATG.GC.C..CGCCTCa                                                                                                       <  1:991651/101‑1 (MQ=255)
     gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAgcgc                                                                                                   <  2:3433266/101‑1 (MQ=255)
     gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAgcgc                                                                                                   >  2:1663967/1‑101 (MQ=255)
      aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGcgcc                                                                                                  >  1:5405108/1‑101 (MQ=255)
        cGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGcgccgc                                                                                                <  2:5013727/101‑1 (MQ=255)
           aaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCt                                                                                             >  2:4691473/1‑101 (MQ=255)
            acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.g                                                                                           <  1:1104576/101‑1 (MQ=255)
             cacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.Ga                                                                                          <  2:263783/101‑1 (MQ=255)
             cacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.Ga                                                                                          >  1:2859937/1‑101 (MQ=255)
             cacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.Ga                                                                                          >  2:3625373/1‑101 (MQ=255)
              acCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.c                                                                                        >  2:2523140/1‑101 (MQ=255)
               ccGTCATCACCACGCACCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.Cg                                                                                       <  2:3767670/101‑1 (MQ=255)
               ccGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.Cg                                                                                       >  1:3803664/1‑101 (MQ=255)
                 gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.Cgcc                                                                                     >  2:2835101/1‑99 (MQ=255)
                    atccCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCt                                                                                  <  1:2190789/101‑1 (MQ=255)
                    atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCt                                                                                  >  1:2607650/1‑101 (MQ=255)
                       accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                               >  1:523486/1‑101 (MQ=255)
                       accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                               >  2:290964/1‑101 (MQ=255)
                         cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccccc                                                                             >  1:1742685/1‑101 (MQ=255)
                          acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                                                                            >  2:901066/1‑101 (MQ=255)
                           cGCAACCGCCCTGCACCCCCGGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcg                                                                           <  2:1346350/101‑1 (MQ=255)
                            gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                          <  2:2355602/101‑1 (MQ=255)
                             cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgcg                                                                         <  1:4773674/101‑1 (MQ=255)
                              aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                        >  2:3552075/1‑101 (MQ=255)
                              aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                        >  2:5043873/1‑101 (MQ=255)
                              aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCCCCCCCCGCgg                                                                        >  2:4718922/1‑101 (MQ=255)
                                     cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                 <  2:3712684/101‑1 (MQ=255)
                                     cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                 <  2:4245535/101‑1 (MQ=255)
                                      ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                               <  1:1010202/91‑1 (MQ=2)
                                        caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                              <  1:1679812/101‑1 (MQ=255)
                                         ccgctgcgTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  1:2078904/94‑1 (MQ=0)
                                          cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            <  1:766838/95‑1 (MQ=2)
                                          ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            >  2:5314202/1‑101 (MQ=255)
                                           ccccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                           <  1:596845/99‑1 (MQ=255)
                                           ccccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAA.CTGACCCGGCAC.C..GGCCTATGCGCTG.GAC.GT.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          >  1:1842902/3‑101 (MQ=2)
                                           aggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          <  2:3980391/96‑1 (MQ=2)
                                            gctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.G.AT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          >  1:1270437/4‑101 (MQ=2)
                                             tgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         <  2:3320313/98‑1 (MQ=2)
                                             agcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          <  1:2670299/98‑1 (MQ=2)
                                              gcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         >  2:5153600/3‑101 (MQ=2)
                                               ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCGCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                       <  1:929925/101‑1 (MQ=255)
                                               cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                        <  1:82644/100‑1 (MQ=2)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGGGGGGAGCGaaaagggg                                                    >  1:3578633/1‑96 (MQ=0)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                     <  1:338403/101‑1 (MQ=2)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                     <  1:5110389/101‑1 (MQ=2)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                      <  1:4196028/101‑2 (MQ=2)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                      >  2:4563723/1‑100 (MQ=2)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                    >  1:2507597/1‑100 (MQ=2)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                    <  2:137027/101‑2 (MQ=2)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                     >  2:1495369/1‑99 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                                  <  2:2253908/101‑7 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGAGGCGTGGATCGAAACaaaa                                                    <  2:3157138/101‑4 (MQ=2)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                                  <  1:3840092/101‑6 (MQ=0)
                                                     cccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                 <  2:4630797/100‑5 (MQ=2)
                                                      ccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGGGGGTCGAAAcggggcgaag                                               >  1:1531939/1‑92 (MQ=2)
                                                      ccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                 >  2:4355957/1‑95 (MQ=0)
                                                      ccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                 <  1:5570144/101‑7 (MQ=0)
                                                       cccccGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                               >  1:433869/1‑95 (MQ=2)
                                                       cccccGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                               >  1:3913391/1‑95 (MQ=2)
                                                       cccccGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                               <  2:5459070/101‑7 (MQ=2)
                                                       cccccGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCCGCGCCGCTCT.CA.CGTCGTTCCTCCCGCGGGTGCGTTTATCTAAActcacgaaga                                               >  2:310365/1‑92 (MQ=21)
                                                         cccGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                             <  1:5667739/101‑9 (MQ=0)
                                                         cccGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                             <  2:550667/101‑1 (MQ=255)
                                                         cccGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                             <  1:1205088/101‑1 (MQ=255)
                                                         cccGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                             >  2:1545276/1‑101 (MQ=255)
                                                          ccGCGGGGGCGTGGATCGAAACAACCCGGTCATG.GC.C..CGCCGCAGCGCCGCGCT.GA.CGCCCCTCCCCCCGCGGGGGCGGGGATCGAAACAACCGCGGTTCg                                            >  2:1872416/1‑101 (MQ=37)
                                                            gcgGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGc                                          >  1:2243043/1‑101 (MQ=255)
                                                             cgGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                         <  1:456993/101‑1 (MQ=255)
                                                             cgGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                         <  1:1440409/101‑1 (MQ=255)
                                                               ggggCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                       <  2:1941575/101‑1 (MQ=255)
                                                                   ggTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                   <  1:3552292/100‑1 (MQ=255)
                                                                   cGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                   <  1:3666405/101‑1 (MQ=255)
                                                                    gTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                  <  2:1714259/101‑1 (MQ=255)
                                                                     tGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                 >  2:4110810/1‑101 (MQ=255)
                                                                     tGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                 >  1:4111341/1‑101 (MQ=255)
                                                                      ggATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCTCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                                >  2:3849294/1‑101 (MQ=255)
                                                                       gATCGAAACAACCCGATCGTT.TC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                               <  2:670665/101‑1 (MQ=255)
                                                                       gATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                               >  2:2158615/1‑101 (MQ=255)
                                                                       gATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAATCCGCGGATCGGCGTGAcccgccc                               >  1:4658313/1‑101 (MQ=255)
                                                                        aTCAAAACACCCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GC.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                              <  2:1167460/101‑1 (MQ=255)
                                                                          cGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccccc                            >  1:4854378/1‑99 (MQ=255)
                                                                            aaaCAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                          >  1:371742/1‑101 (MQ=255)
                                                                            aaaCAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                          <  2:5688214/101‑1 (MQ=255)
                                                                              acaacCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                        <  2:3822088/101‑1 (MQ=255)
                                                                              acaacCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                        >  2:2408911/1‑101 (MQ=255)
                                                                               caacCCGATCATG.GC.C..CGCCTGAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc                       >  1:3278616/1‑101 (MQ=255)
                                                                                accccGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                      <  1:2313890/99‑1 (MQ=255)
                                                                                 acCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg                     <  2:734497/101‑1 (MQ=255)
                                                                                    cGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCg                  >  2:2663564/1‑101 (MQ=255)
                                                                                     gATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCCTCGGTCGc                 >  2:4907532/1‑101 (MQ=255)
                                                                                      aTCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                >  2:3248869/1‑101 (MQ=255)
                                                                                        cATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc              <  1:3074553/101‑1 (MQ=255)
                                                                                         aTG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc             <  1:5025471/101‑1 (MQ=255)
                                                                                              gac..cGCCGCAGCGCCGCGCT.GA.CGACGCACCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc          <  1:2794470/99‑1 (MQ=255)
                                                                                              c.c..cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcg         <  1:243880/101‑1 (MQ=255)
                                                                                              c.c..cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcg         <  1:3342108/101‑1 (MQ=255)
                                                                                                   cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgcg       <  1:2144085/101‑1 (MQ=255)
                                                                                                    cccTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGAgg      <  2:813017/100‑1 (MQ=255)
                                                                                                        cAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc  <  2:4180287/101‑1 (MQ=255)
                                                                                                        |                                                                                                      
GCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1397452‑1397652

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG