Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,556 G→A 20.6% A480T (GCC→AGC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5560G→A20.6% 171.0 ‑2.4 ‑0.0305A480T (GCC→ACC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (24/39):  ref base (123/118):  total (147/158)
Fisher's exact test strand distribution p-value = 8.88e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.17e-01

Only 100 of 345 total aligned reads displayed.

GGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1397456‑1397656
                                                                                                         |                                                                                                      
ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAgcg                                                                                                        >  2:2391789/1‑101 (MQ=255)
 gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAgcgc                                                                                                       >  1:3710900/1‑101 (MQ=255)
 gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAgcgc                                                                                                       >  2:25384/1‑101 (MQ=255)
   tCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGcgccg                                                                                                     >  2:1021237/1‑101 (MQ=255)
      aaaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCgcgc                                                                                                  >  2:339084/1‑101 (MQ=255)
      aaaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCgcgc                                                                                                  >  2:74260/1‑101 (MQ=255)
       aaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCt                                                                                                 >  2:4691473/1‑101 (MQ=255)
        acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.g                                                                                               <  1:1104576/101‑1 (MQ=255)
         cacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCtcg                                                                                               >  2:243847/1‑99 (MQ=255)
           ccGTCATCACCACGCACCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.Cg                                                                                           <  2:3767670/101‑1 (MQ=255)
            cGTCATCCCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.ctt                                                                                          <  1:3844756/101‑3 (MQ=255)
            cGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGt                                                                                          >  2:5025190/1‑101 (MQ=255)
                atccCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCt                                                                                      <  1:2190789/101‑1 (MQ=255)
                atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCt                                                                                      <  1:3822898/101‑1 (MQ=255)
                atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCt                                                                                      >  1:2607650/1‑101 (MQ=255)
                  caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                                                                                    <  2:5085233/101‑1 (MQ=255)
                   accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                                   >  1:523486/1‑101 (MQ=255)
                      acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                                                                                >  2:2313764/1‑101 (MQ=255)
                      acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                                                                                <  1:5364708/101‑1 (MQ=255)
                      acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                                                                                >  2:901066/1‑101 (MQ=255)
                         cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgcg                                                                             <  1:4773674/101‑1 (MQ=255)
                          aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                            >  2:5380486/1‑101 (MQ=255)
                          aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                            >  2:3552075/1‑101 (MQ=255)
                          aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCAGCgg                                                                            <  2:4897496/101‑1 (MQ=255)
                              gCCCGGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                        <  1:3957730/101‑1 (MQ=255)
                                 cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                     <  2:3712684/101‑1 (MQ=255)
                                 cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                     <  2:4245535/101‑1 (MQ=255)
                                  ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                   <  1:1010202/91‑1 (MQ=2)
                                   gCACCACCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                   <  2:5194685/101‑1 (MQ=255)
                                    gcgcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.G.AT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  2:1775035/94‑1 (MQ=0)
                                    caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  1:1679812/101‑1 (MQ=255)
                                      tcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 <  2:5621194/95‑1 (MQ=0)
                                      cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                <  1:766838/95‑1 (MQ=2)
                                       ccccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                               <  1:596845/99‑1 (MQ=255)
                                       ccccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAA..CTGACCCGGCAC.C..GGCCTATGCGCTG.GAC.GT.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              >  1:1842902/3‑101 (MQ=2)
                                       accgcgTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCGATGATCAAA.TC.C..GCAATCGGCCGGGCTCG.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                <  1:1553335/96‑1 (MQ=0)
                                        gctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.G.AT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              >  1:1270437/4‑101 (MQ=2)
                                         tgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             <  2:3320313/98‑1 (MQ=2)
                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCGCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                           <  1:929925/101‑1 (MQ=255)
                                           cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            <  1:82644/100‑1 (MQ=2)
                                           cgTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            <  2:4198967/100‑1 (MQ=2)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcggg                                                         >  2:4707655/1‑98 (MQ=2)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                           >  1:3595309/1‑101 (MQ=2)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                          <  2:4417526/101‑1 (MQ=255)
                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                         <  1:338403/101‑1 (MQ=2)
                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                          <  1:4196028/101‑2 (MQ=2)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                        >  1:2507597/1‑100 (MQ=2)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                         <  1:3035318/101‑3 (MQ=2)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                         >  2:1224800/1‑99 (MQ=2)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                         <  2:1390842/101‑3 (MQ=2)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                         <  1:2082591/101‑3 (MQ=2)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                        >  2:1553241/1‑98 (MQ=2)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGAGGCGTGGATCGAAACaaaa                                                        <  2:3157138/101‑4 (MQ=2)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                       >  1:1189406/1‑101 (MQ=255)
                                                 tCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                     >  1:2855559/1‑97 (MQ=2)
                                                 cccccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                     <  2:4630797/100‑5 (MQ=2)
                                                  ccccccGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaag                                                   <  2:3833086/101‑10 (MQ=2)
                                                  ccccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                     <  1:5570144/101‑7 (MQ=0)
                                                  ccccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                                    >  1:3733821/1‑101 (MQ=255)
                                                   cccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                                   >  1:433869/1‑95 (MQ=2)
                                                   cccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCCGCGCCGCTCT.CA.CGTCGTTCCTCCCGCGGGTGCGTTTATCTAAActcacgaaga                                                   >  2:310365/1‑92 (MQ=21)
                                                    ccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                                  <  2:1969322/101‑1 (MQ=255)
                                                     cccGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                                 <  1:5667739/101‑9 (MQ=0)
                                                      ccGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCg                                                <  2:2187822/101‑1 (MQ=255)
                                                        gcgGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGc                                              <  1:1745408/101‑1 (MQ=255)
                                                        gcgGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGc                                              >  1:2243043/1‑101 (MQ=255)
                                                         cgGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                             <  1:1440409/101‑1 (MQ=255)
                                                         cgGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                             >  1:4616252/1‑101 (MQ=255)
                                                          gggggCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                            >  2:4101642/1‑101 (MQ=255)
                                                            gggCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGCCCCCACCCCGCCGGGGGCGGGGATCGAAAGAACCGCGGATCGGCGCGa                                          >  2:1353659/1‑101 (MQ=255)
                                                               cGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                       >  2:1898290/1‑101 (MQ=255)
                                                                 tGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                     >  2:5476236/1‑101 (MQ=255)
                                                                 tGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                     >  2:4110810/1‑101 (MQ=255)
                                                                  ggATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCTCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                                    >  2:3849294/1‑101 (MQ=255)
                                                                  ggATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccccc                                    >  2:723943/1‑101 (MQ=255)
                                                                   gATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                   >  2:4969/1‑101 (MQ=255)
                                                                   gATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAATCCGCGGATCGGCGTGAcccgccc                                   >  1:4658313/1‑101 (MQ=255)
                                                                        aaaCAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgcccccccc                              >  2:2656548/1‑101 (MQ=255)
                                                                         aacaac.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa                             >  2:1680940/1‑101 (MQ=255)
                                                                           caac.CCGATCATG.GC.C..CGCCTGAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc                           >  1:3278616/1‑101 (MQ=255)
                                                                           caac.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCacc                           >  2:1497255/1‑99 (MQ=255)
                                                                            acc.ccGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                          <  1:2313890/99‑1 (MQ=255)
                                                                                 cGATCCTG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCg                      <  2:2850440/101‑1 (MQ=255)
                                                                                 cGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGACCCGCCCCGCCCATCGGGCg                      >  2:2743840/1‑101 (MQ=255)
                                                                                   aTCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                    >  2:3248869/1‑101 (MQ=255)
                                                                                   aTCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                    <  2:3598319/101‑1 (MQ=255)
                                                                                     cATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc                  <  1:3074553/101‑1 (MQ=255)
                                                                                      aTG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                 <  1:5025471/101‑1 (MQ=255)
                                                                                      aTG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                 >  1:2660663/1‑101 (MQ=255)
                                                                                      aTG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                 >  1:4835748/1‑101 (MQ=255)
                                                                                       tG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccc                >  2:4572133/1‑101 (MQ=255)
                                                                                        g.gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccccc               <  1:3554506/101‑1 (MQ=255)
                                                                                          gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc              <  1:5083292/101‑1 (MQ=255)
                                                                                           gac..cGCCGCAGCGCCGCGCT.GA.CGACGCACCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc              <  1:2794470/99‑1 (MQ=255)
                                                                                           c.c..cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcg             <  1:3342108/101‑1 (MQ=255)
                                                                                           c.c..cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcg             <  1:243880/101‑1 (MQ=255)
                                                                                                cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgcg           <  1:2144085/101‑1 (MQ=255)
                                                                                                   cTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg        >  2:2912373/1‑101 (MQ=255)
                                                                                                    tCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCggggg       >  2:5792056/1‑101 (MQ=255)
                                                                                                      aGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCg     >  2:868676/1‑101 (MQ=255)
                                                                                                         |                                                                                                      
GGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1397456‑1397656

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG