Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,563 T→C 11.3% L482P (CTG→ACC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5630T→C11.3% 73.8 ‑0.7 ‑1.3300L482P (CTG→CCG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/20):  ref base (127/138):  total (141/159)
Fisher's exact test strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.24e-02

Only 100 of 339 total aligned reads displayed.

AACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1397463‑1397663
                                                                                                        |                                                                                                      
gctcttccgatctCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCt                                                                                                        <  2:410508/92‑1 (MQ=38)
 acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.g                                                                                                      <  1:1104576/101‑1 (MQ=255)
  cacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCtcg                                                                                                      >  2:243847/1‑99 (MQ=255)
     cGTCATCCCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.ctt                                                                                                 <  1:3844756/101‑3 (MQ=255)
         atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCt                                                                                             >  1:2607650/1‑101 (MQ=255)
          tcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTc                                                                                            <  1:2169665/101‑1 (MQ=255)
          ccaccacGCAACCCCCCGGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTc                                                                                            <  2:5481547/100‑1 (MQ=255)
           cactacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcc                                                                                           >  2:4322439/1‑101 (MQ=255)
              cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTccccc                                                                                        >  1:1742685/1‑101 (MQ=255)
               acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcccccc                                                                                       >  2:901066/1‑101 (MQ=255)
                cGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcg                                                                                      <  1:380966/101‑1 (MQ=255)
                 gCAACCGCCCTGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                                     <  2:3729998/101‑1 (MQ=255)
                       gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                               >  1:796788/1‑101 (MQ=255)
                       gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                               <  1:474413/101‑1 (MQ=255)
                       gCCCGGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                               <  1:3957730/101‑1 (MQ=255)
                         ccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGt                                                                             >  2:3666177/1‑101 (MQ=255)
                           ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                          <  1:1010202/91‑1 (MQ=2)
                            gCACCACCGGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACAACCCAAAAATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                          <  1:4810009/101‑1 (MQ=38)
                            gCACCACCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                          <  2:5194685/101‑1 (MQ=255)
                             caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                         <  2:3292588/101‑1 (MQ=255)
                              ccgctgcgTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  1:2078904/94‑1 (MQ=0)
                               tcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  2:5621194/95‑1 (MQ=0)
                               cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                       <  2:682534/95‑1 (MQ=2)
                               ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                       >  2:5314202/1‑101 (MQ=255)
                                cgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAAT.CG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                      <  1:2242879/97‑1 (MQ=0)
                                 cccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                     <  2:4689789/100‑1 (MQ=255)
                                   cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                   >  1:516151/1‑101 (MQ=255)
                                    cgTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                   <  2:4198967/100‑1 (MQ=2)
                                     gacGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                                 >  2:3492002/3‑100 (MQ=255)
                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcggg                                                                >  2:4707655/1‑98 (MQ=2)
                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                 <  1:5595504/101‑1 (MQ=255)
                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGGGGGGAGCGaaaagggg                                                               >  1:3578633/1‑96 (MQ=0)
                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                                 <  1:4196028/101‑2 (MQ=2)
                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                                 >  2:4563723/1‑100 (MQ=2)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                               >  1:2507597/1‑100 (MQ=2)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                               <  1:1586032/101‑2 (MQ=2)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                                >  2:1495369/1‑99 (MQ=2)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                                <  2:1390842/101‑3 (MQ=2)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGGGGGTCGAAAcggggcg                                                             >  2:2311462/1‑95 (MQ=0)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcga                                                              >  2:3816304/1‑99 (MQ=2)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                               >  2:1553241/1‑98 (MQ=2)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGAGGCGTGGATCGAAACaaaa                                                               <  2:3157138/101‑4 (MQ=2)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                              >  1:1189406/1‑101 (MQ=255)
                                         cTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                                                             <  1:3055915/101‑4 (MQ=2)
                                         cTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                                                             <  1:1499886/101‑4 (MQ=2)
                                         cTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATcgat                                                             >  2:3565456/1‑98 (MQ=2)
                                         cTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaac                                                              >  1:749210/1‑97 (MQ=2)
                                         cTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                                              <  2:5069185/101‑5 (MQ=2)
                                         cTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                                              >  1:2312889/1‑97 (MQ=2)
                                         cTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgc                                                             <  1:4905232/101‑1 (MQ=255)
                                          tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                            >  1:2855559/1‑97 (MQ=2)
                                          tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCT.GT.C..GGCCGCGAACACCGATAC.CG.CGGCGCTCCCCCCGCGGGGGCGGGGATCGAAACATcgttc                                                            >  2:2835365/1‑97 (MQ=2)
                                          cccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                            <  2:5013856/100‑5 (MQ=2)
                                          cccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                            <  2:4630797/100‑5 (MQ=2)
                                           ccccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                                           >  1:3733821/1‑101 (MQ=255)
                                            cccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                                          >  1:433869/1‑95 (MQ=2)
                                             ccccGCGGGGGCGTTGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                                         <  2:3852165/101‑1 (MQ=255)
                                             ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                                         <  2:1969322/101‑1 (MQ=255)
                                             ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                                         <  2:4136939/101‑1 (MQ=255)
                                             ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                                         <  1:2780411/101‑1 (MQ=255)
                                                  cgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                                    <  1:456993/101‑1 (MQ=255)
                                                    ggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                                  >  1:543436/1‑101 (MQ=255)
                                                     gggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                                 >  1:337808/1‑101 (MQ=255)
                                                     gggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGCCCCCACCCCGCCGGGGGCGGGGATCGAAAGAACCGCGGATCGGCGCGa                                                 >  2:1353659/1‑101 (MQ=255)
                                                       gCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcc                                               <  2:3090147/101‑1 (MQ=255)
                                                        ggTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                              <  1:3552292/100‑1 (MQ=255)
                                                        cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                              >  2:2660237/1‑101 (MQ=255)
                                                        cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                              <  1:3666405/101‑1 (MQ=255)
                                                          tGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                            >  2:3841246/1‑101 (MQ=255)
                                                            gATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                          >  1:1271606/1‑101 (MQ=255)
                                                            gATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                          >  2:2158615/1‑101 (MQ=255)
                                                            gATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAATCCGCGGATCGGCGTGAcccgccc                                          >  1:4658313/1‑101 (MQ=255)
                                                             aTCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTACCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                         <  1:2144080/101‑1 (MQ=255)
                                                             aTCAAAACACCCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GC.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                         <  2:1167460/101‑1 (MQ=255)
                                                                  aacaacCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa                                    >  2:1680940/1‑101 (MQ=255)
                                                                    caacCCGATCATGGC.C..CGCCTG.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc                                  >  1:3278616/1‑101 (MQ=255)
                                                                      acCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg                                <  2:734497/101‑1 (MQ=255)
                                                                         cGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGACCCGCCCCGCCCATCGGGCg                             >  2:2743840/1‑101 (MQ=255)
                                                                          gATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACATCGGCGGATCGGGGTGACCCGCCCCGCCCATCGGTCGc                            >  2:2564177/1‑101 (MQ=255)
                                                                          gATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCCTCGGTCGc                            >  2:4907532/1‑101 (MQ=255)
                                                                          gATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGGCGc                            >  1:5062284/1‑101 (MQ=255)
                                                                           aTCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                           >  2:2368872/1‑101 (MQ=255)
                                                                              aTGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                        >  1:2660663/1‑101 (MQ=255)
                                                                              aTGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                        >  1:1826338/1‑101 (MQ=255)
                                                                              aTGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                        >  1:97823/1‑101 (MQ=255)
                                                                                ggC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCT.CCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc                     <  1:873876/101‑1 (MQ=255)
                                                                                  c.c..cGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcg                    <  1:3342108/101‑1 (MQ=255)
                                                                                  c.c..cGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcg                    <  1:243880/101‑1 (MQ=255)
                                                                                     cactgcTC.ACCGCCGCGCG.AG.CGTGTCTCCACCCGCGGGGGCGTCGACCGAAACTTCCGCAGATTGGCTTTTCCCGCCCCGCCCATCGGCCGCTCCCCCcg                    <  2:1821172/96‑1 (MQ=255)
                                                                                          cTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg               >  2:2912373/1‑101 (MQ=255)
                                                                                          cTC.AGCGCCGCGCG.GA.CGTCGCCCCCCCCGCGGGGGGGTGGATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg               <  1:5780207/101‑1 (MQ=38)
                                                                                           tC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCggggg              >  2:5792056/1‑101 (MQ=255)
                                                                                           tC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGCCGCTCCCCCCCCggggg              >  1:5245690/1‑101 (MQ=255)
                                                                                            c.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc             <  1:290997/101‑1 (MQ=255)
                                                                                              aGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCg            >  2:868676/1‑101 (MQ=255)
                                                                                                 gccgcGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTgg         >  2:4797708/1‑101 (MQ=255)
                                                                                                   cgcgCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGAt       <  2:4420476/101‑1 (MQ=255)
                                                                                                    gcgcT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATc      <  2:5404818/101‑1 (MQ=255)
                                                                                                    gcgcT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCCTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATc      <  2:1921486/101‑1 (MQ=255)
                                                                                                     cgcT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCg     >  2:3166668/1‑101 (MQ=255)
                                                                                                        |                                                                                                      
AACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1397463‑1397663

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG