Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,564 G→C 11.2% L482L (CTG→ACC RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5640G→C11.2% 70.6 ‑0.6 ‑0.7300L482L (CTG→CTCRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (13/18):  ref base (124/131):  total (142/158)
Fisher's exact test strand distribution p-value = 5.69e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.24e-01

Only 100 of 339 total aligned reads displayed.

ACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1397464‑1397664
                                                                                                         |                                                                                                     
acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.g                                                                                                       <  1:1104576/101‑1 (MQ=255)
 cacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.Ga                                                                                                      >  1:2859937/1‑101 (MQ=255)
  cccGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.c                                                                                                    <  1:3801799/100‑1 (MQ=255)
     gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTc                                                                                                 >  2:2120496/1‑101 (MQ=255)
      tcatcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCg                                                                                                >  1:836117/1‑101 (MQ=255)
       catcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGc                                                                                               <  1:769372/101‑1 (MQ=255)
        atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCt                                                                                              <  1:3822898/101‑1 (MQ=255)
         tcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTc                                                                                             <  1:2169665/101‑1 (MQ=255)
         ccaccacGCAACCCCCCGGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTc                                                                                             <  2:5481547/100‑1 (MQ=255)
             cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTccccc                                                                                         >  1:1742685/1‑101 (MQ=255)
              acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcccccc                                                                                        >  2:901066/1‑101 (MQ=255)
               cGCAACCGCCCTGCACCCCCGGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcg                                                                                       <  2:1346350/101‑1 (MQ=255)
                gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                                      <  2:2355602/101‑1 (MQ=255)
                      gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                                <  1:474413/101‑1 (MQ=255)
                        ccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGt                                                                              >  2:3666177/1‑101 (MQ=255)
                         cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                             <  2:4245535/101‑1 (MQ=255)
                          ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                           <  1:1010202/91‑1 (MQ=2)
                           gCACCACCGGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACAACCCAAAAATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                           <  1:4810009/101‑1 (MQ=38)
                           gCACCACCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                           <  2:5194685/101‑1 (MQ=255)
                            caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                          <  2:3292588/101‑1 (MQ=255)
                             ccgctgcgTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                         <  1:2078904/94‑1 (MQ=0)
                              tcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                         <  2:5621194/95‑1 (MQ=0)
                              cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                        <  2:682534/95‑1 (MQ=2)
                              ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                        >  2:5314202/1‑101 (MQ=255)
                               cgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAAT.CG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                       <  1:2242879/97‑1 (MQ=0)
                                 ctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAAT.CG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                     >  1:5776906/3‑101 (MQ=2)
                                 agcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                      <  1:2670299/98‑1 (MQ=2)
                                  cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                    >  1:516151/1‑101 (MQ=255)
                                    gacGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                                  >  2:3492002/3‑100 (MQ=255)
                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcggg                                                                 >  2:4707655/1‑98 (MQ=2)
                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                  >  2:1663266/1‑101 (MQ=2)
                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                                   >  1:3595309/1‑101 (MQ=2)
                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                  >  2:1055317/1‑101 (MQ=255)
                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                  <  2:4417526/101‑1 (MQ=255)
                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                  <  1:5595504/101‑1 (MQ=255)
                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                                 <  1:338403/101‑1 (MQ=2)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                                <  1:1586032/101‑2 (MQ=2)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                                <  2:137027/101‑2 (MQ=2)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                                 >  2:4682133/1‑99 (MQ=2)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                                 <  1:3035318/101‑3 (MQ=2)
                                       gCTCCCCCCGCGGGGGCGTGGGTCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcga                                                               >  1:3286248/1‑99 (MQ=2)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                                              <  2:2253908/101‑7 (MQ=2)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                                <  2:4518772/101‑4 (MQ=2)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGAGGCGTGGATCGAAACaaaa                                                                <  2:3157138/101‑4 (MQ=2)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                               >  1:1189406/1‑101 (MQ=255)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                                                              <  1:1499886/101‑4 (MQ=2)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATcgat                                                              >  2:3565456/1‑98 (MQ=2)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                                               <  2:5069185/101‑5 (MQ=2)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                                               >  1:2312889/1‑97 (MQ=2)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgc                                                              <  1:4905232/101‑1 (MQ=255)
                                         tCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaa                                                            <  2:1792929/101‑9 (MQ=2)
                                         tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCT.GT.C..GGCCGCGAACACCGATAC.CG.CGGCGCTCCCCCCGCGGGGGCGGGGATCGAAACATcgttc                                                             >  2:2835365/1‑97 (MQ=2)
                                         cccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                             <  2:5013856/100‑5 (MQ=2)
                                          ccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaag                                                           <  2:3833086/101‑10 (MQ=2)
                                           cccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                                           >  1:702922/1‑95 (MQ=2)
                                             cccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                                         >  2:1545276/1‑101 (MQ=255)
                                                 cgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                                     >  1:4616252/1‑101 (MQ=255)
                                                  gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                                    >  2:4101642/1‑101 (MQ=255)
                                                   ggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                                   <  2:1941575/101‑1 (MQ=255)
                                                   ggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                                   >  1:543436/1‑101 (MQ=255)
                                                      gCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcc                                                <  2:3090147/101‑1 (MQ=255)
                                                       cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                               >  2:1898290/1‑101 (MQ=255)
                                                       cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                               >  2:2660237/1‑101 (MQ=255)
                                                        ggggATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                              <  2:1492902/99‑1 (MQ=255)
                                                         tGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                             >  1:4111341/1‑101 (MQ=255)
                                                          ggATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCTCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                                            >  2:3849294/1‑101 (MQ=255)
                                                           gATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                           >  1:1271606/1‑101 (MQ=255)
                                                            aTCGAATCAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                          <  1:973383/101‑1 (MQ=255)
                                                            aTCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTACCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                          <  1:2144080/101‑1 (MQ=255)
                                                             tCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                                         >  2:4626226/1‑101 (MQ=255)
                                                                aaaCAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                                      >  1:371742/1‑101 (MQ=255)
                                                                aaaCAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                                      >  1:3850196/1‑101 (MQ=255)
                                                                 aacaacCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa                                     <  1:3349709/101‑1 (MQ=255)
                                                                  acaccCCGATCAGGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                                    <  1:2523282/101‑1 (MQ=255)
                                                                    aacCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                                  >  1:4208906/1‑101 (MQ=255)
                                                                     acCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg                                 >  2:2436555/1‑101 (MQ=255)
                                                                     acCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg                                 <  2:734497/101‑1 (MQ=255)
                                                                        cGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGACCCGCCCCGCCCATCGGGCg                              >  2:2743840/1‑101 (MQ=255)
                                                                         gATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACATCGGCGGATCGGGGTGACCCGCCCCGCCCATCGGTCGc                             >  2:2564177/1‑101 (MQ=255)
                                                                         gATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCCTCGGTCGc                             >  2:4907532/1‑101 (MQ=255)
                                                                         gATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGGCGc                             >  1:5062284/1‑101 (MQ=255)
                                                                            cATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc                          <  1:3074553/101‑1 (MQ=255)
                                                                             aTGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                         >  1:1826338/1‑101 (MQ=255)
                                                                             aTGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                         <  1:2467645/101‑1 (MQ=255)
                                                                               ggC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccccc                       <  1:3554506/101‑1 (MQ=255)
                                                                                gC.C..CGCCTC.GGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc                      <  2:3713451/101‑1 (MQ=255)
                                                                                 gac..cGCCGC.AGCGCCGCGCT.GA.CGACGCACCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc                      <  1:2794470/99‑1 (MQ=255)
                                                                                       gCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCCCgg                  >  1:2638766/1‑101 (MQ=255)
                                                                                        ccTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCggg                 <  1:4340221/101‑1 (MQ=255)
                                                                                         cTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg                >  2:2912373/1‑101 (MQ=255)
                                                                                          tC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCggggg               >  2:5792056/1‑101 (MQ=255)
                                                                                          tC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGCCGCTCCCCCCCCggggg               >  1:5245690/1‑101 (MQ=255)
                                                                                           c.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc              <  2:4180287/101‑1 (MQ=255)
                                                                                             aGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCg             >  2:868676/1‑101 (MQ=255)
                                                                                                gccgcGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTgg          >  2:4797708/1‑101 (MQ=255)
                                                                                                   gcgcT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATc       <  2:5404818/101‑1 (MQ=255)
                                                                                                    cgcT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCg      >  2:3166668/1‑101 (MQ=255)
                                                                                                     gcT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa     >  2:4125947/1‑101 (MQ=255)
                                                                                                       t.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa   >  2:3786541/1‑101 (MQ=255)
                                                                                                         gA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc  <  1:4658911/101‑1 (MQ=255)
                                                                                                         |                                                                                                     
ACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1397464‑1397664

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG