Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,565 A→C 12.6% T483P (ACG→CGG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5650A→C12.6% 74.6 ‑2.0 ‑0.6299T483P (ACG→CCG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (11/21):  ref base (111/109):  total (141/158)
Fisher's exact test strand distribution p-value = 1.29e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.41e-01

Only 100 of 339 total aligned reads displayed.

CACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT  >  NC_014034/1397465‑1397665
                                                                                                          |                                                                                                    
  ccGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACg                                                                                                    >  1:3803664/1‑101 (MQ=255)
    gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTc                                                                                                  >  2:2120496/1‑101 (MQ=255)
       atccCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCt                                                                                               <  1:2190789/101‑1 (MQ=255)
        ccaccacGCAACCCCCCGGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTc                                                                                              <  2:5481547/100‑1 (MQ=255)
          accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTccc                                                                                            <  1:3321922/101‑1 (MQ=255)
             acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTcccccc                                                                                         >  2:901066/1‑101 (MQ=255)
              cGCAACCGCCCTGCACCCCCGGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCcg                                                                                        <  2:1346350/101‑1 (MQ=255)
               gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCcgc                                                                                       <  2:2355602/101‑1 (MQ=255)
                cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCcgcg                                                                                      <  1:4773674/101‑1 (MQ=255)
                     gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGc                                                                                 >  1:796788/1‑101 (MQ=255)
                       ccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGt                                                                               >  2:3666177/1‑101 (MQ=255)
                        cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg                                                                              <  2:4245535/101‑1 (MQ=255)
                         ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTT.CACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                            <  1:1010202/91‑1 (MQ=2)
                          gCACCACCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGa                                                                            <  2:5194685/101‑1 (MQ=255)
                           caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                           <  2:3292588/101‑1 (MQ=255)
                             tcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                          <  2:5621194/95‑1 (MQ=0)
                             cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                         <  2:682534/95‑1 (MQ=2)
                             ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                         >  2:5314202/1‑101 (MQ=255)
                              ccccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAA..CTGACCCGGCAC.C..GGCCTA.TGCGCTG.GACGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                       >  1:1842902/3‑101 (MQ=2)
                                gatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTT.CACGGCGGGCTATCGTCGCTCCCCCCCCGGGGGCGTGGATCGAAAc                                                                     >  1:2699345/4‑101 (MQ=2)
                                   gacGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                                   >  2:3492002/3‑100 (MQ=255)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTT.CACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcggg                                                                  >  2:4707655/1‑98 (MQ=2)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                   >  2:1663266/1‑101 (MQ=2)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                   <  1:4666627/101‑1 (MQ=2)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                   >  2:1055317/1‑101 (MQ=255)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                   <  2:4417526/101‑1 (MQ=255)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCCGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                   <  1:531319/101‑1 (MQ=255)
                                   gTCGCACCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAA.TC.C..GCAATC.GGCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                                                    <  1:4623812/101‑2 (MQ=2)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                                  >  1:247383/1‑101 (MQ=2)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTT.CACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGGGGGTCGaaaaggggc                                                                >  1:4491237/1‑95 (MQ=2)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                                 <  2:137027/101‑2 (MQ=2)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                                  >  1:1613898/1‑99 (MQ=2)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                                  >  2:4682133/1‑99 (MQ=2)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                                  <  1:2082591/101‑3 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTT.CACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                                               <  2:2253908/101‑7 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                                 <  1:2802386/101‑4 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                                 <  2:4518772/101‑4 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCTGCGGTCGCTCCCCCCGCGGAGGCGTGGATCGAAACaaaa                                                                 <  2:3157138/101‑4 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                                >  1:2574482/1‑101 (MQ=255)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATcgat                                                               >  2:3565456/1‑98 (MQ=2)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaac                                                                >  1:749210/1‑97 (MQ=2)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                                                <  2:5069185/101‑5 (MQ=2)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTT.CACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaa                                                             <  2:1792929/101‑9 (MQ=2)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCT..GT.C..GGCCGCGAACACCGATACCGCGGCGCTCCCCCCGCGGGGGCGGGGATCGAAACATcgttc                                                              >  2:2835365/1‑97 (MQ=2)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                                               <  1:3840092/101‑6 (MQ=0)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                                              >  1:5073642/1‑101 (MQ=255)
                                        cccccccGCGGGGGCGTGGATCGAATCAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                                              <  1:4164889/100‑1 (MQ=255)
                                        cccccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                              <  2:5013856/100‑5 (MQ=2)
                                         ccccccGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTT.CACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaag                                                            <  2:3833086/101‑10 (MQ=2)
                                         ccccccGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTT.CACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGGGGGTCGAAAcggggcgaag                                                            >  1:1531939/1‑92 (MQ=2)
                                         ccccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatga                                                             >  1:495962/1‑96 (MQ=2)
                                         ccccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGC.AGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                              <  1:5570144/101‑7 (MQ=0)
                                         ccccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                                             >  1:3729148/1‑101 (MQ=255)
                                          cccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                                            <  1:3565673/101‑7 (MQ=2)
                                          cccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                                            >  1:3913391/1‑95 (MQ=2)
                                            cccGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.G.AACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                                          <  1:5667739/101‑9 (MQ=0)
                                            cccGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                                          >  2:1545276/1‑101 (MQ=255)
                                            cccGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                                          <  1:1205088/101‑1 (MQ=255)
                                               gcgGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGc                                                       <  1:1745408/101‑1 (MQ=255)
                                               gcgGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGc                                                       >  1:2243043/1‑101 (MQ=255)
                                                cgGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                                      <  1:1440409/101‑1 (MQ=255)
                                                 gggggCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                                     >  2:4101642/1‑101 (MQ=255)
                                                 gggggCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                                     <  1:3144757/101‑1 (MQ=255)
                                                  ggggCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                                    <  2:1941575/101‑1 (MQ=255)
                                                    ggCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                                  >  1:2903989/1‑101 (MQ=255)
                                                     gCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcc                                                 <  2:3090147/101‑1 (MQ=255)
                                                      cGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                                >  2:2660237/1‑101 (MQ=255)
                                                      cGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                                >  2:1898290/1‑101 (MQ=255)
                                                       ggggATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                               <  2:1492902/99‑1 (MQ=255)
                                                         ggATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCTCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                                             >  2:3849294/1‑101 (MQ=255)
                                                           aTCGAATCAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                           <  1:973383/101‑1 (MQ=255)
                                                            tCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                                          >  1:303692/1‑101 (MQ=255)
                                                            tCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                                          >  2:4626226/1‑101 (MQ=255)
                                                                 acaac.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgcccccccccc                                     >  1:4264022/1‑99 (MQ=255)
                                                                   acc.ccGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                                   <  1:2313890/99‑1 (MQ=255)
                                                                    ac.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg                                  <  2:734497/101‑1 (MQ=255)
                                                                    ac.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg                                  >  2:2436555/1‑101 (MQ=255)
                                                                        cGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGACCCGCCCCGCCCATCGGGCg                               >  2:2743840/1‑101 (MQ=255)
                                                                         gATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACATCGGCGGATCGGGGTGACCCGCCCCGCCCATCGGTCGc                              >  2:2564177/1‑101 (MQ=255)
                                                                         gATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCCTCGGTCGc                              >  2:4907532/1‑101 (MQ=255)
                                                                          aTCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCCCCCCTCGGTcccc                             >  1:1110946/1‑98 (MQ=255)
                                                                             aTG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcct                          <  1:5195753/101‑2 (MQ=255)
                                                                             aTG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                          >  1:3611044/1‑101 (MQ=255)
                                                                             aTG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                          >  1:4835748/1‑101 (MQ=255)
                                                                              tG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccc                         <  1:339128/101‑1 (MQ=255)
                                                                               g.gC.C..CGCCTC.AGCGCCGCGCTGACGTCGCT.CCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc                       <  1:873876/101‑1 (MQ=255)
                                                                                 gC.C..CGCCTC.GGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc                       <  2:3713451/101‑1 (MQ=255)
                                                                                       cGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgcg                    <  1:2144085/101‑1 (MQ=255)
                                                                                          cTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg                 >  2:2912373/1‑101 (MQ=255)
                                                                                          cTC.AGCGCCGCGCGGACGTCGCCCCCCCCGCGGGGGGGTGGATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg                 <  1:5780207/101‑1 (MQ=38)
                                                                                           tC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCggggg                >  2:5792056/1‑101 (MQ=255)
                                                                                            c.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc               <  2:4180287/101‑1 (MQ=255)
                                                                                            c.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc               >  1:2649485/1‑101 (MQ=255)
                                                                                              aGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCg              >  2:868676/1‑101 (MQ=255)
                                                                                                 gccgcGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTgg           >  2:4797708/1‑101 (MQ=255)
                                                                                                   cgcgCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGAt         <  1:3374959/101‑1 (MQ=255)
                                                                                                    gcgcTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATc        <  2:5404818/101‑1 (MQ=255)
                                                                                                     cgcTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCg       >  2:3166668/1‑101 (MQ=255)
                                                                                                      gcTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa      >  2:4125947/1‑101 (MQ=255)
                                                                                                        tGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa    >  2:3786541/1‑101 (MQ=255)
                                                                                                          |                                                                                                    
CACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTC.AGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT  >  NC_014034/1397465‑1397665

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG