Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,443,778 G→C 100% A33A (GCC→GCG RCAP_rcc01340 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,443,7780G→C100.0% 856.7 226A33A (GCC→GCGRCAP_rcc01340LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (121/105):  ref base (0/0):  total (121/105)

Only 100 of 236 total aligned reads displayed.

GCCGCGCCCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGGTCGATGCGCCGCGAGATCGTGGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTGGCTCCA  >  NC_014034/1443680‑1443875
                                                                                                  |                                                                                                 
gccgcgccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGCTCGTCGc                                                                                                 >  1:2309779/1‑101 (MQ=255)
gccgcgccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGc                                                                                                 >  1:1823298/1‑101 (MQ=255)
gccgcgccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCCCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGc                                                                                                 <  1:5262435/101‑1 (MQ=255)
 ccgcgctCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCa                                                                                                <  1:929726/101‑1 (MQ=255)
 ccgcgccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCa                                                                                                <  1:2231233/101‑1 (MQ=255)
   gcgccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAAc                                                                                              >  1:1674668/1‑101 (MQ=255)
    cgccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACg                                                                                             >  1:3911666/1‑101 (MQ=255)
        cTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCaa                                                                                         <  1:2634217/101‑1 (MQ=255)
         tGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAg                                                                                        <  1:1992485/101‑1 (MQ=255)
          ggtcggtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGa                                                                                       <  1:5377662/101‑1 (MQ=255)
          ggtcggtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGa                                                                                       <  2:666719/101‑1 (MQ=255)
          ggtcggtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGa                                                                                       >  2:4241995/1‑101 (MQ=255)
           gtcggtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGAc                                                                                      <  1:203555/101‑1 (MQ=255)
            tcggtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGAcc                                                                                     >  2:297079/1‑101 (MQ=255)
            tcggtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGAcc                                                                                     >  2:182016/1‑101 (MQ=255)
            gcggtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGCcc                                                                                     <  1:3294460/100‑1 (MQ=255)
             cggtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGAccc                                                                                    >  2:1835703/1‑101 (MQ=255)
             cggtcAGGTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGAccc                                                                                    <  1:3683378/101‑1 (MQ=255)
               gtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAg                                                                                  >  2:2117910/1‑101 (MQ=255)
               gtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAg                                                                                  >  2:5466278/1‑101 (MQ=255)
               gtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAg                                                                                  <  1:4030901/101‑1 (MQ=255)
                 ccgTTCGTGCCCGGTCTGGCGGCCCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTc                                                                                <  2:4018917/99‑1 (MQ=255)
                 cAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTc                                                                                <  2:4440930/101‑1 (MQ=255)
                 cAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTc                                                                                >  1:1081485/1‑101 (MQ=255)
                  aGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcg                                                                               <  1:4015413/101‑1 (MQ=255)
                  aGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcg                                                                               <  2:1427666/101‑1 (MQ=255)
                  aGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcg                                                                               >  2:3245840/1‑101 (MQ=255)
                   gTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcgc                                                                              <  2:2240528/101‑1 (MQ=255)
                     tccgatctggTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcgccg                                                                            <  2:2924463/93‑1 (MQ=255)
                      cGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCATGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcgccgc                                                                           <  1:4935461/101‑1 (MQ=255)
                      cGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcgccgc                                                                           <  1:5670960/101‑1 (MQ=255)
                      cGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcgccgc                                                                           >  1:5304493/1‑101 (MQ=255)
                        tGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcgccgcgc                                                                         >  2:3218900/1‑101 (MQ=255)
                         gCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcgccgcgcc                                                                        >  1:4670965/1‑101 (MQ=255)
                          cccGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcgccgcgccg                                                                       >  1:1863627/1‑101 (MQ=255)
                             ggTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcgccgagatcgga                                                                    >  1:2924630/1‑93 (MQ=255)
                               tCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGccc                                                                  >  1:1055147/1‑101 (MQ=255)
                                 tGGCGGCGCAGGAACAGGGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTg                                                                <  2:2611949/101‑1 (MQ=255)
                                  ggcggcGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGc                                                               >  1:4260784/1‑101 (MQ=255)
                                       cgcAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGt                                                          >  2:896158/1‑101 (MQ=255)
                                       cgcAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGt                                                          <  2:2604391/101‑1 (MQ=255)
                                         cAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGc                                                        >  2:2934091/1‑101 (MQ=255)
                                         cAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGc                                                        >  1:2878334/1‑101 (MQ=255)
                                          aGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGcc                                                       >  2:5575475/1‑101 (MQ=255)
                                           ggAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGccc                                                      >  2:5041652/1‑101 (MQ=255)
                                            gAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCt                                                     <  1:999120/101‑1 (MQ=255)
                                            gAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCt                                                     >  2:544484/1‑101 (MQ=255)
                                            gAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCt                                                     >  2:3042873/1‑101 (MQ=255)
                                            gAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCt                                                     >  2:1611658/1‑101 (MQ=255)
                                             accAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTc                                                    <  1:4269904/99‑1 (MQ=255)
                                             aaCAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTc                                                    >  1:607736/1‑101 (MQ=255)
                                              aCAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTcg                                                   <  1:1727845/101‑1 (MQ=255)
                                                 gCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGTCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTcgcgc                                                <  1:4465400/101‑1 (MQ=255)
                                                   ggCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCgcgcgc                                              >  1:2625248/1‑101 (MQ=255)
                                                     cAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgcca                                            <  1:2714244/101‑1 (MQ=255)
                                                     cAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgcca                                            <  1:4360187/101‑1 (MQ=255)
                                                      aGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccag                                           >  2:4265797/1‑101 (MQ=255)
                                                      aGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccag                                           >  2:3691314/1‑101 (MQ=255)
                                                      aGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccag                                           >  1:3376193/1‑101 (MQ=255)
                                                         ccGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccagcgc                                        >  2:3532251/1‑101 (MQ=255)
                                                          cGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccagcgcc                                       >  1:5066587/1‑101 (MQ=255)
                                                          cGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccagcgcc                                       >  1:4199073/1‑101 (MQ=255)
                                                           gAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccagcgcca                                      >  1:313302/1‑101 (MQ=255)
                                                            aGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGcgccagcgccag                                     >  2:4822911/1‑101 (MQ=255)
                                                            aGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGcgccagcgccag                                     <  2:1068620/101‑1 (MQ=255)
                                                             gCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGa                                    <  1:3092288/101‑1 (MQ=255)
                                                               gTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGaaa                                  >  1:242786/1‑101 (MQ=255)
                                                                tCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAAc                                 >  1:1143276/1‑101 (MQ=255)
                                                                 cGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGCCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCCCCAGCGCCAGAAACa                                >  2:4934441/1‑101 (MQ=255)
                                                                  gCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAg                               <  1:5775752/101‑1 (MQ=255)
                                                                  gCCTCCATCCGCTCGATGCGCCGCGAGATCGTCACAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAg                               <  1:5026194/101‑1 (MQ=255)
                                                                    cTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGcc                             >  1:76500/1‑101 (MQ=255)
                                                                     tCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCg                            >  2:4740911/1‑101 (MQ=255)
                                                                     tCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCg                            <  2:847204/101‑1 (MQ=255)
                                                                      ccATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCTTGCCCTCGCGCGCCAGCGCCAGAAACACCCgg                           >  1:5507777/1‑101 (MQ=255)
                                                                      ccATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCgg                           >  2:5321259/1‑101 (MQ=255)
                                                                        aTCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGAt                         <  1:5590738/101‑1 (MQ=255)
                                                                        aTCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGAt                         <  1:1234165/101‑1 (MQ=255)
                                                                        aTCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCCCCGCGCCGCGCCCTGCAGCGTGCCCCCGCGCGCCAGCGCCGGAAACAGCCGGag                         >  1:4369373/1‑100 (MQ=255)
                                                                          ccGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATgt                       <  2:4622535/101‑1 (MQ=255)
                                                                           cgctCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATgtc                      <  2:2878173/98‑1 (MQ=255)
                                                                           cgctCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATgtc                      <  2:1943486/98‑1 (MQ=255)
                                                                             ctCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATgtcgt                    <  1:4761741/100‑1 (MQ=255)
                                                                               cGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTcc                  >  2:3226326/1‑101 (MQ=255)
                                                                               cGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTcc                  >  2:643288/1‑101 (MQ=255)
                                                                                gATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTccc                 <  2:3911424/101‑1 (MQ=255)
                                                                                gATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTccc                 >  1:1346694/1‑101 (MQ=255)
                                                                                gATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTccc                 >  1:1742959/1‑101 (MQ=255)
                                                                                gATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTccc                 >  2:1530830/1‑101 (MQ=255)
                                                                                 aTGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCa                >  1:1568850/1‑101 (MQ=255)
                                                                                    cgccgcTAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGtt             <  2:2676427/101‑1 (MQ=255)
                                                                                    cgccgcGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGtt             >  2:3960229/1‑101 (MQ=255)
                                                                                     gccgcGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTc            <  1:280507/101‑1 (MQ=255)
                                                                                       cgcgAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCtg          <  1:4844523/101‑1 (MQ=255)
                                                                                        gcgAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCtgt         <  1:4499347/101‑1 (MQ=255)
                                                                                          gagaTCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTgg       >  1:2117731/1‑101 (MQ=255)
                                                                                            gaTCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTGGCt     <  1:3553010/101‑1 (MQ=255)
                                                                                             aTCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTGGCTc    >  1:728861/1‑101 (MQ=255)
                                                                                               cGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTGGCTCCa  >  1:827563/1‑101 (MQ=255)
                                                                                               cGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTGGCTCCa  >  2:3614441/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCCGCGCCCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGGTCGATGCGCCGCGAGATCGTGGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTGGCTCCA  >  NC_014034/1443680‑1443875

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG