Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,613,981 C→T 2.0% intergenic (+144/+83) rnd2 → / ← uvrD ribonuclease D/DNA helicase II

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,613,9810C→T 2.0% 2.0 ‑1.2 ‑0.0202intergenic (+144/+83)rnd2/uvrDribonuclease D/DNA helicase II
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (77/120):  total (77/125)
Fisher's exact test strand distribution p-value = 3.00e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 240 total aligned reads displayed.

TCCCCCTGCCCGCCGAGGGCCCAAAGG.CCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGT  >  NC_014034/1613882‑1614080
                                                                                                       |                                                                                                       
ccccccGCCCCGCGGGGGCCCCAAGGG.CCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTgcg                                                                                                        <  2:3100132/100‑1 (MQ=14)
         ccgcagGGGGCCCAAGGC.CCCTGGCCCGGCCCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGaa                                                                                              <  1:2314340/101‑1 (MQ=14)
          cgccgAGGGCCCAAGGG.CCCGCGGCCGGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGaaa                                                                                             <  1:1078202/101‑1 (MQ=255)
            ccgGGGGCCAAAAGG.CCCTCGGCCTGCCCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACg                                                                                           <  1:756997/101‑1 (MQ=255)
              gAGGGCCCAAAGG.CCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGGGGGCCTCCGCCGCCCGGGGGGGGGGGCG.GGC.CCTTTTTGGCC.TTGGGGAGACgg                                                                                          >  2:1600561/1‑101 (MQ=2)
               ggggCCCAAGGG.CCCGGGGCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTc                                                                                        <  2:5655925/100‑1 (MQ=255)
                 ggCCCAAGGC.CCCTCGGCCTGCCCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCtt                                                                                      <  1:3971400/101‑1 (MQ=255)
                  gCCAAAAGC.CCCTCGGCCTGCCCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTg                                                                                     <  1:3218177/101‑1 (MQ=255)
                   gccAAAGGCCCCGCGCCCTGCCCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTg                                                                                     <  2:5430168/100‑1 (MQ=17)
                     cAAAGG.CCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGGGCGTTCGCGCCC.CCGGGGGGGGGGGCA.GGG.CCTTTTTGCGC.CTGGGGGAACGGGCTTGCcg                                                                                  >  1:1602446/1‑101 (MQ=14)
                          gccccTCGGCCGGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.c                                                                             <  1:725699/99‑1 (MQ=255)
                            cccTGGGCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTg                                                                           <  2:915166/101‑1 (MQ=255)
                              cTCGCCCTGCCCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGtt                                                                         <  2:3048157/101‑1 (MQ=255)
                                  gCCTGCGCCCGCCCCGCCATCGGCGGGGGGGCTATCGCCG.CCCGGGCGGGGCGCC.CGG.CCTTGTTTCGCGTTGGGGGAACGGCTTTTCCCCGG.cgatggga                                                                      >  2:4373311/1‑94 (MQ=2)
                                   ccTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.At                                                                   <  2:1282190/101‑1 (MQ=255)
                                    cggcccccgccccgcCATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.g                                                                 <  1:1983451/96‑1 (MQ=255)
                                    cTGCGCCCGCCCCGCCATCGGCGGGGGCCTTCGCGCCC.CCCGGGGGGGGGGGC.GGC.CCTTTTTTTGC.TTTGGGGGAAGGGCTTTCCGCGG.CGGGTGCgggg                                                                   >  1:4301544/1‑98 (MQ=2)
                                    cTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.g                                                                 <  2:4650752/101‑1 (MQ=255)
                                     tGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCC.CCCGGGGGGGGCGCC.GGG.CCTTGTTGCGC.TTTGGGGAAAGGGCTTGCCGCGG.GCGTTGCG.AT.gc                                                                >  2:1842993/1‑101 (MQ=12)
                                       cccccgccccgcCATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.gcgg                                                              <  1:5717836/99‑1 (MQ=255)
                                       cccccgccccgcCATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.gcgg                                                              <  1:2090687/99‑1 (MQ=255)
                                        gcccgcccCGCCATCGGCGGGGGCCTTTGCGCCC.CCCCGGCGGGGGGCAGGGCGCTTTTTTG.GC.TTGGGG.GACGGGCTTGCCGCGGCCGGTTGCG.AT.gcgg                                                              >  2:5335852/1‑101 (MQ=9)
                                          ccgccccgccATCGGCGGGCGCATTCGCGCCG.CGGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.gcgggcg                                                           <  1:4980658/101‑1 (MQ=255)
                                              cccgccATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGa                                                       <  2:2243641/101‑1 (MQ=255)
                                              cccgccATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGa                                                       <  2:5044444/101‑1 (MQ=255)
                                               ccgccATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGaa                                                      <  2:1087752/101‑1 (MQ=255)
                                               ccgccATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGaa                                                      <  2:3181003/101‑1 (MQ=255)
                                               ccgccATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGaa                                                      <  1:2784494/101‑1 (MQ=255)
                                                cgccATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGGCGCC.GGC.CCTTGTTGTGC.TTGGGGGAACGGTCTTGCCGCGG.CTGTTGCG.GC.GCGGGGGGGGgaa                                                     >  2:2281255/1‑101 (MQ=17)
                                                 gccATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAg                                                    <  2:144421/101‑1 (MQ=255)
                                                 gccATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAg                                                    <  2:1314760/101‑1 (MQ=255)
                                                 gccATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAg                                                    <  1:1202975/101‑1 (MQ=255)
                                                 gccATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAg                                                    <  2:5267774/101‑1 (MQ=255)
                                                 gccATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAg                                                    <  2:2046651/101‑1 (MQ=255)
                                                    aTCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCa                                                 <  1:3903042/101‑1 (MQ=255)
                                                       ggcgggcgCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTg                                              >  2:2629936/1‑101 (MQ=255)
                                                       ggcgggcgCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTg                                              >  1:5624592/1‑101 (MQ=255)
                                                        gcgggcgCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGc                                             <  2:3593092/101‑1 (MQ=255)
                                                           ggcgCCTTCGCGCCG.CCGGGGCGGAACGCA.GGC.CTTTGTTGCCC.TTGGGGAAACGGTCTCGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCt                                          <  2:5229887/101‑1 (MQ=18)
                                                           ggcgCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCt                                          <  2:4988220/101‑1 (MQ=255)
                                                           ggcgCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCt                                          >  2:2858560/1‑101 (MQ=255)
                                                            gcgcATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCtt                                         >  2:5104687/1‑101 (MQ=255)
                                                            gcgcATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCtt                                         <  2:2426168/101‑1 (MQ=255)
                                                             cgcATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTc                                        <  1:1958163/101‑1 (MQ=255)
                                                              gcATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTcc                                       >  2:842430/1‑101 (MQ=255)
                                                              gcATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTcc                                       <  1:626638/101‑1 (MQ=255)
                                                               tccgggggcag.ccgGGGCGGGTAAAA.GGC.CTCTGTCGTGC.TTGGGGAAACGGTCTTGGCACAG.GTGATGCG.CCAGCGGGCGGGGAAAGGCAGTGCCTTTcc                                       <  2:5733859/91‑1 (MQ=21)
                                                               cATTCGCGCCG.CCGTGGCGGGACGCA.CGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCt                                      <  2:779887/101‑1 (MQ=255)
                                                               cATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGGAAGGCAGGGCCCTTCCt                                      >  1:3395746/1‑101 (MQ=255)
                                                               cATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCt                                      >  2:4222560/1‑101 (MQ=255)
                                                               cATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCt                                      >  1:3614900/1‑101 (MQ=255)
                                                                aTTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGGAAGGCAGTGCCCTTCCTg                                     >  1:1144406/1‑101 (MQ=255)
                                                                 ttCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGa                                    <  1:1013916/101‑1 (MQ=255)
                                                                   cgcgCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATc                                  >  1:4037830/1‑101 (MQ=255)
                                                                    cgcggcgggggggGGGGACAAA.GGC.CTCTGTCGTGC.TTGGGGAAACGGTCTTGGCACAG.GTGATGCGCCA.GCGGGCGGGGAAAGGCAGTGCCTTTCCCGAt                                   <  1:606066/92‑1 (MQ=25)
                                                                      gccg.ccGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGc                               <  2:4056490/101‑1 (MQ=255)
                                                                       ggccggggggCGGGCCAAA.GGC.CTTTGTCGTGC.TTGGGGAAACGGTCTTGGCACAG.GTGATGCGCCG.GCGGGCGCGGAAAGGCAGTGCCTTTCCCGATCCg                                <  1:5185760/95‑1 (MQ=18)
                                                                        cg.ccgGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGccc                             >  2:2596466/1‑101 (MQ=255)
                                                                        cg.ccgGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGccc                             <  1:257474/101‑1 (MQ=255)
                                                                            cgGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGaa                          >  2:2978950/1‑101 (MQ=255)
                                                                             ggggCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAAc                         >  1:2136825/1‑101 (MQ=255)
                                                                             ggggCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAAc                         >  2:3390435/1‑101 (MQ=255)
                                                                             ggggCGGGACGCA.GCC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAAc                         <  1:4624854/101‑1 (MQ=255)
                                                                               ggCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCg                       <  2:4591804/101‑1 (MQ=255)
                                                                               ggCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCg                       >  2:732406/1‑101 (MQ=255)
                                                                               ggCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCg                       <  2:3946390/101‑1 (MQ=255)
                                                                                gCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCgg                      >  1:1210024/1‑101 (MQ=255)
                                                                                gCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCgg                      <  1:3145408/101‑1 (MQ=255)
                                                                                gCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCACTGCCCTTCCTGATCCGCCCGAACCgg                      >  1:4862175/1‑101 (MQ=255)
                                                                                 cGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCggg                     <  1:1434506/101‑1 (MQ=255)
                                                                                 cGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCggg                     <  1:5467519/101‑1 (MQ=255)
                                                                                      cGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCa                >  2:869187/1‑101 (MQ=255)
                                                                                       gCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAg               <  2:3845736/101‑1 (MQ=255)
                                                                                       gCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAg               >  1:1730762/1‑101 (MQ=255)
                                                                                       gCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAg               <  1:4122967/101‑1 (MQ=255)
                                                                                        cA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGa              >  1:2462332/1‑101 (MQ=255)
                                                                                        cA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGa              <  1:4759498/101‑1 (MQ=255)
                                                                                         a.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGaa             >  2:937305/1‑101 (MQ=255)
                                                                                         a.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGaa             >  1:4913682/1‑101 (MQ=255)
                                                                                            gC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACg           <  2:4781582/101‑1 (MQ=255)
                                                                                            gC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACg           <  1:2908609/101‑1 (MQ=255)
                                                                                            gC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACg           >  2:5491783/1‑101 (MQ=255)
                                                                                             c.cTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACgg          >  1:3836002/1‑101 (MQ=255)
                                                                                               cTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGc         >  2:4303205/1‑101 (MQ=255)
                                                                                               cTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGc         <  2:2916506/101‑1 (MQ=255)
                                                                                               cTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGc         <  2:1231986/101‑1 (MQ=255)
                                                                                                tttGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGct        <  2:4861353/101‑2 (MQ=255)
                                                                                                tttGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCa        <  1:2764985/101‑1 (MQ=255)
                                                                                                tttGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCa        <  1:3311490/101‑1 (MQ=255)
                                                                                                 ttgttgCGC.TTGGGGAAACGGTCGTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCCAACCGGGGCTCAGAACGGCAc       <  1:985065/101‑1 (MQ=255)
                                                                                                  tgttgCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcg      >  1:388996/1‑101 (MQ=255)
                                                                                                  tgttgCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcg      <  1:889292/101‑1 (MQ=255)
                                                                                                  tgttgCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcg      >  2:4501499/1‑101 (MQ=255)
                                                                                                   gttgCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcgt     >  2:5791041/1‑101 (MQ=255)
                                                                                                    ttgCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcgtc    >  2:560992/1‑101 (MQ=255)
                                                                                                    ttgCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcgtc    >  1:3039333/1‑101 (MQ=255)
                                                                                                    gggcgc.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcgtc    >  2:3365069/3‑101 (MQ=255)
                                                                                                     tgCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcgtcg   <  1:4493154/101‑1 (MQ=255)
                                                                                                      gcgc.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcgtcgt  <  1:5108592/101‑1 (MQ=255)
                                                                                                      gcgc.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcgtcgt  >  1:3725782/1‑101 (MQ=255)
                                                                                                       |                                                                                                       
TCCCCCTGCCCGCCGAGGGCCCAAAGG.CCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCG.CCGGGGCGGGACGCA.GGC.CTTTGTTGCGC.TTGGGGAAACGGTCTTGCCGCGG.CTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGT  >  NC_014034/1613882‑1614080

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG