Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,786,541 C→T 2.7% A225A (GCC→GCT RCAP_rcc01648 → ABC transporter permease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,786,5410C→T 2.7% 8.5 ‑0.3 ‑0.1259A225A (GCC→GCTRCAP_rcc01648ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (4/3):  ref base (120/132):  total (124/135)
Fisher's exact test strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.65e-01

Only 100 of 275 total aligned reads displayed.

TGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CT.GG.CCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATCC  >  NC_014034/1786444‑1786641
                                                                                                    |                                                                                                    
tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGGCTtt                                                                                                    <  2:3784146/101‑1 (MQ=255)
tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGGCTtt                                                                                                    >  1:5377062/1‑101 (MQ=255)
   tGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGt                                                                                                >  1:3464038/1‑101 (MQ=255)
    tccGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTa                                                                                               <  2:2603983/100‑1 (MQ=255)
       gCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATcc                                                                                            <  2:1209911/101‑1 (MQ=255)
        cATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCa                                                                                           >  2:5371967/1‑101 (MQ=255)
          tCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATc                                                                                         <  2:5216060/101‑1 (MQ=255)
           cTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCt                                                                                        <  2:5049118/101‑1 (MQ=255)
           cTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCt                                                                                        >  1:5244786/1‑101 (MQ=255)
           cTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCt                                                                                        >  2:1333962/1‑101 (MQ=255)
            tGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTg                                                                                       >  2:5551577/1‑101 (MQ=255)
            tGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTg                                                                                       >  1:988387/1‑101 (MQ=255)
                 gccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAttt                                                                                  <  2:852142/101‑1 (MQ=255)
                   cgcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTcc                                                                                >  1:1533783/1‑101 (MQ=255)
                   cgcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTcc                                                                                <  1:5000525/101‑1 (MQ=255)
                     cggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAg                                                                              >  2:5659182/1‑101 (MQ=255)
                      ggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGa                                                                             >  2:2020835/1‑101 (MQ=255)
                       gcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGAt                                                                            >  1:4181637/1‑101 (MQ=255)
                        cgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATg                                                                           <  2:4115968/101‑1 (MQ=255)
                        cgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATg                                                                           <  1:482247/101‑1 (MQ=255)
                          cgggcgggGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGtt                                                                         <  2:4328037/101‑1 (MQ=255)
                           gggcggggCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGACTTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTc                                                                        >  2:1101228/1‑101 (MQ=255)
                           gggcggggCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTc                                                                        >  2:5469010/1‑101 (MQ=255)
                            ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                       <  2:178930/101‑1 (MQ=255)
                            ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                       <  1:1944047/101‑1 (MQ=255)
                            ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                       >  1:2771482/1‑101 (MQ=255)
                             gcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGa                                                                      >  1:2316965/1‑101 (MQ=255)
                              cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                     >  1:3147635/7‑101 (MQ=255)
                               ggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACa                                                                    >  2:2635923/6‑101 (MQ=255)
                               ggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACa                                                                    >  2:3846798/6‑101 (MQ=255)
                               ggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACa                                                                    >  2:5501572/6‑101 (MQ=255)
                               ggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACa                                                                    <  1:5551869/96‑1 (MQ=255)
                                 ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAtt                                                                  <  1:1456014/98‑1 (MQ=255)
                                 ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAtt                                                                  <  1:299176/98‑1 (MQ=255)
                                  ggTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTg                                                                 <  2:1585978/100‑1 (MQ=255)
                                  gctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTg                                                                 <  1:5611247/99‑1 (MQ=255)
                                   ctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGc                                                                <  2:4848888/100‑1 (MQ=255)
                                     ttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCg                                                              >  2:619682/1‑101 (MQ=255)
                                     ttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCg                                                              <  1:4209968/101‑1 (MQ=255)
                                      tCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGa                                                             >  1:598312/1‑101 (MQ=255)
                                       cGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAg                                                            >  2:5454380/1‑101 (MQ=255)
                                       cGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAg                                                            <  1:4483547/101‑1 (MQ=255)
                                        gCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAgg                                                           >  2:1043808/1‑101 (MQ=255)
                                        gCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAgg                                                           >  2:3199349/1‑101 (MQ=255)
                                        gCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAgg                                                           >  2:291341/1‑101 (MQ=255)
                                            aTCTGGAAGGTCGTGCTGGTGGTCGAATTT.CT.GGTCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCt                                                       <  1:4966639/101‑1 (MQ=255)
                                             tCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTc                                                      >  2:1412189/1‑101 (MQ=255)
                                             tCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTc                                                      <  2:210125/101‑1 (MQ=255)
                                             tCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTc                                                      <  1:1005126/101‑1 (MQ=255)
                                              cTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCg                                                     <  1:3264551/101‑1 (MQ=255)
                                              cTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCg                                                     <  1:5082521/101‑1 (MQ=255)
                                              cTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCg                                                     <  2:1932287/101‑1 (MQ=255)
                                               tGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGc                                                    >  1:689196/1‑101 (MQ=255)
                                               tGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGc                                                    >  1:5293680/1‑101 (MQ=255)
                                                ggAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGcc                                                   <  1:4703592/101‑1 (MQ=255)
                                                 gAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCt                                                  <  1:869034/101‑1 (MQ=255)
                                                 gAAGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCt                                                  >  1:5387460/1‑101 (MQ=255)
                                                  aaGGTCGTGCTGTTAGTCGGATTTCCG.GG.CCGCTCGAGCGGGATAGG.CTTTCGTATCCATCTGAATTGCCAGATGTTCGACATTGCCGAGGTGCTCGCCTg                                                 <  2:5041263/101‑1 (MQ=255)
                                                  aaGGTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTg                                                 <  1:2636072/101‑1 (MQ=255)
                                                     gTCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggc                                              >  2:2129174/1‑101 (MQ=255)
                                                      tCGTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcg                                             <  1:5498971/101‑1 (MQ=255)
                                                        gTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                           <  2:5093083/101‑1 (MQ=255)
                                                        gTGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                           >  1:659275/1‑101 (MQ=255)
                                                         tGCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggcg                                          <  1:3031918/101‑1 (MQ=255)
                                                          gCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggcgg                                         <  1:5659477/101‑1 (MQ=255)
                                                          gCTGGTGGTCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggcgg                                         <  2:3650391/101‑1 (MQ=255)
                                                             ggtggtCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCtt                                      >  2:303725/1‑101 (MQ=255)
                                                              gtggtCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCttt                                     >  2:1702167/1‑101 (MQ=255)
                                                               tggtCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTc                                    >  2:4387241/1‑101 (MQ=255)
                                                               tggtCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTc                                    >  2:5428075/1‑101 (MQ=255)
                                                                 gtCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGt                                  >  2:668631/1‑101 (MQ=255)
                                                                 gtCGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGt                                  <  2:3616902/101‑1 (MQ=255)
                                                                   cGAATTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCt                                <  2:4865326/101‑1 (MQ=255)
                                                                     aaTTT.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGc                              >  1:411747/1‑101 (MQ=255)
                                                                       ttt.CTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGt                            >  1:4670663/1‑101 (MQ=255)
                                                                         ttctggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTg                           >  1:2159863/6‑101 (MQ=255)
                                                                           cgggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGAt                         <  2:3934105/98‑1 (MQ=255)
                                                                               g.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTc                     <  2:3147446/101‑1 (MQ=255)
                                                                               g.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTc                     <  1:3963146/101‑1 (MQ=255)
                                                                                 ccGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCg                    <  2:1036242/101‑1 (MQ=255)
                                                                                  cGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGc                   <  1:1666334/101‑1 (MQ=255)
                                                                                   gCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGcc                  >  2:5187962/1‑101 (MQ=255)
                                                                                    cTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCg                 <  1:2916018/101‑1 (MQ=255)
                                                                                    cTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCg                 <  2:3831864/101‑1 (MQ=255)
                                                                                    cTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCg                 <  2:2349068/101‑1 (MQ=255)
                                                                                    cTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCg                 >  1:1776443/1‑101 (MQ=255)
                                                                                       gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg              >  2:347508/1‑101 (MQ=255)
                                                                                       gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg              >  1:3589957/1‑101 (MQ=255)
                                                                                        aaCGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGa             <  2:1533690/101‑1 (MQ=255)
                                                                                           gggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATg          >  2:335945/1‑101 (MQ=255)
                                                                                            ggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATgg         <  1:2271998/101‑1 (MQ=255)
                                                                                              aTCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCt       >  1:740624/1‑101 (MQ=255)
                                                                                              aTCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGCCTGCGTGATGCTCGCCGTCGAATGGCt       >  1:4599425/1‑101 (MQ=255)
                                                                                                tcggcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTg      >  1:3788136/5‑101 (MQ=255)
                                                                                                tcggcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTg      <  2:2714721/97‑1 (MQ=255)
                                                                                                 cggcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGa     >  1:3615631/4‑101 (MQ=255)
                                                                                                  ggcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGAt    >  1:1100733/3‑101 (MQ=255)
                                                                                                  ggcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGAt    <  2:2940909/99‑1 (MQ=255)
                                                                                                   gcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATc   >  2:460241/2‑101 (MQ=255)
                                                                                                   gcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATc   >  1:1315382/2‑101 (MQ=255)
                                                                                                    |                                                                                                    
TGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CT.GG.CCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATCC  >  NC_014034/1786444‑1786641

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG