Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,965,239 G→T 100% intergenic (+7/+85) bdhA → / ← RCAP_rcc01818 3‑hydroxybutyrate dehydrogenase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,965,2390G→T100.0% 279.4 75intergenic (+7/+85)bdhA/RCAP_rcc018183‑hydroxybutyrate dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (36/39):  ref base (0/0):  total (36/39)

TGGGGGGAACGACGGTCTTCCTGTGTGCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...GCGGG.CT..CCG..CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGG  >  NC_014034/1965141‑1965331
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tGGGGGGAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTtcg                                                                                                     <  1:5560827/101‑1 (MQ=255)
  gggggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGcc                                                                                                   >  2:3724395/1‑101 (MQ=255)
  gggggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGcc                                                                                                   >  2:281938/1‑101 (MQ=255)
  gggggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGcc                                                                                                   <  2:1753507/101‑1 (MQ=255)
   ggggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGGGGCTGGATGGCGCTTGGAGAGATTtcgctg                                                                                                  >  2:714593/1‑99 (MQ=255)
   ggggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCt                                                                                                  >  2:2773984/1‑101 (MQ=255)
   ggggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCt                                                                                                  >  1:2258453/1‑101 (MQ=255)
    gggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTc                                                                                                 <  1:4432817/101‑1 (MQ=255)
    gggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTc                                                                                                 <  1:291836/101‑1 (MQ=255)
     ggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCg                                                                                                <  2:3139242/101‑1 (MQ=255)
     ggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCg                                                                                                >  1:4969984/1‑101 (MQ=255)
     ggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCg                                                                                                <  1:3899478/101‑1 (MQ=255)
      gAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCgg                                                                                               >  1:16484/1‑101 (MQ=255)
       aaCGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTTGCCTCggg                                                                                              >  2:5390795/1‑101 (MQ=255)
       aaCGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCggg                                                                                              >  2:2136616/1‑101 (MQ=255)
       aaCGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCggg                                                                                              <  2:4053074/101‑1 (MQ=255)
       aaCGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCggg                                                                                              <  2:2812289/101‑1 (MQ=255)
       aaCGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCggg                                                                                              >  2:1516781/1‑101 (MQ=255)
       aaCGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCggg                                                                                              >  1:221587/1‑101 (MQ=255)
       aaCGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCggg                                                                                              >  2:837981/1‑101 (MQ=255)
         cgacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGc                                                                                            >  2:1292420/1‑101 (MQ=255)
         cgacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGc                                                                                            >  2:1031908/1‑101 (MQ=255)
          gacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCa                                                                                           >  2:4680744/1‑101 (MQ=255)
          gacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCa                                                                                           <  2:4959385/101‑1 (MQ=255)
           acgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCaa                                                                                          >  1:3715735/1‑101 (MQ=255)
           acgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCaa                                                                                          >  1:2382886/1‑101 (MQ=255)
            cgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAg                                                                                         >  2:3891471/1‑101 (MQ=255)
            cgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAg                                                                                         <  2:2153145/101‑1 (MQ=255)
            cgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAg                                                                                         <  2:4624665/101‑1 (MQ=255)
            cgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAg                                                                                         <  1:4831209/101‑1 (MQ=255)
             ggTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGc                                                                                        >  2:5658216/1‑101 (MQ=255)
             ggTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGc                                                                                        <  2:116425/101‑1 (MQ=255)
              gTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGcc                                                                                       <  2:4851160/101‑1 (MQ=255)
               tCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGccc                                                                                      <  1:3498872/101‑1 (MQ=255)
               tCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGccc                                                                                      <  2:5753629/101‑1 (MQ=255)
               tCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGccc                                                                                      <  1:1537037/101‑1 (MQ=255)
               tCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGccc                                                                                      <  1:5238999/101‑1 (MQ=255)
               tCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGccc                                                                                      <  1:5266101/101‑1 (MQ=255)
                cTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCg                                                                                     <  2:651780/101‑1 (MQ=255)
                cTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCg                                                                                     <  2:2910888/101‑1 (MQ=255)
                 ttCCTGTGTTCCGAGGCGGCGGCGCAGGTCGCCGGCGCGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGa                                                                                    <  1:2203031/101‑1 (MQ=255)
                 ttCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGa                                                                                    <  1:885866/101‑1 (MQ=255)
                  tCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAg                                                                                   <  1:233896/101‑1 (MQ=255)
                   ccTGTGTTCCGAGGCGGCGGCGCAGATCACTGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAgg                                                                                  >  2:4885515/1‑101 (MQ=255)
                   ccTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAgg                                                                                  <  1:3787043/101‑1 (MQ=255)
                    cTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGc                                                                                 <  1:4335926/101‑1 (MQ=255)
                     tgtgttCGGGGGGGGCGGCGCAGATCACCGGCACGACGTTTTCTGTGGATGGCGGCTGGCCGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCg                                                                                <  2:1091122/101‑1 (MQ=255)
                     tgtgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCg                                                                                <  1:1606643/101‑1 (MQ=255)
                     tgtgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCg                                                                                <  1:3493276/101‑1 (MQ=255)
                     cgatctccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCg                                                                                <  2:3592263/95‑1 (MQ=255)
                      gtgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGGCGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGa                                                                               >  2:2947083/1‑101 (MQ=255)
                      gtgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGa                                                                               <  1:2455786/101‑1 (MQ=255)
                       tgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGAc                                                                              <  2:4274076/101‑1 (MQ=255)
                       tgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGAc                                                                              >  1:1055818/1‑101 (MQ=255)
                       tgttCCGAGGCGGCGGCCCAGATCACCGGCACGACGTTTTCTGCGGATGGCGGCTGGCCGGCGCTCTGAGGGGCTTCGCCTCGGGGCACGCCCCAGGCGAc                                                                              >  2:2677647/1‑101 (MQ=255)
                        gttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGAcc                                                                             >  1:827266/4‑101 (MQ=255)
                        gttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGAcc                                                                             <  2:1394783/98‑1 (MQ=255)
                        gttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTAAGAGATTTATCCTCGGGGCAAGCCCGAggaaacc                                                                             <  2:180273/98‑6 (MQ=255)
                         ttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGAccc                                                                            <  2:5676659/99‑1 (MQ=255)
                         ttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGAccc                                                                            <  2:1955134/99‑1 (MQ=255)
                         ttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGAccc                                                                            <  2:317030/99‑1 (MQ=255)
                         gtccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGAccc                                                                            <  2:2853308/99‑1 (MQ=255)
                          tccGAGGCGGCGGCGCAGATCCCCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg                                                                           <  1:5092804/100‑1 (MQ=255)
                          tccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTGGGCCCGGGGCAAGCCCGAGGGGACccg                                                                           >  2:742932/2‑101 (MQ=255)
                          tccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg                                                                           <  2:4400020/100‑1 (MQ=255)
                          tccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg                                                                           <  2:5177413/100‑1 (MQ=255)
                           ccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGagatcg                                                                          >  1:3592480/1‑96 (MQ=255)
                            cGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg.cc                                                                        >  1:73354/1‑101 (MQ=255)
                             gAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg.ccc                                                                       >  2:1234282/1‑100 (MQ=255)
                              aGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg.cccc                                                                      >  1:276331/1‑99 (MQ=255)
                              aGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg.ccac                                                                      >  1:835726/1‑99 (MQ=255)
                                           gATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCGCCC...GGGGG.CG..CCC..cccc                                                  >  2:419049/1‑101 (MQ=38)
                                            aTCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...GGGGG.GG..CCT..cccccg                                                >  2:1708443/1‑99 (MQ=38)
                                             tCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...CCGGGCCT..CCC..cccccc                                                >  2:4682411/1‑99 (MQ=38)
                                                ccGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...GGGGG.GG..CCG..cgccccccgc                                            >  2:3308862/1‑95 (MQ=37)
                                                 cGGAACGACGATTTCTGTGGATGCCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CG...GGGGG.CG..CAG..ccccccggacc                                           >  2:939848/1‑94 (MQ=21)
                                                     acgacgATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...GGGGG.GG..CCGC.CCCCCGAccccccg                                        >  2:3781504/1‑99 (MQ=25)
                                                         cgaTTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...GGGGG.GTGCCCC..CCCCGGCccccccggga                                     >  2:5703908/1‑96 (MQ=21)
                                                             ttgtgggggtGGCGGGTGGGCGGGGCGGGGAGAGATTCCCCCGCGGCGCAACCCGGGGCCCCCCCG.CC...GGGGG.CT..CCGC.CCCCGGACCCCCGAGGTATTTc                                <  2:4655465/92‑1 (MQ=2)
                                                                                  ggCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...GGGGG.CT..CCGC.CCCCGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGgcg           >  2:2864647/1‑101 (MQ=21)
                                                                                  ggCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...CGGGG.GT..CCGCCCCCCGGACCCCC.AGGGATTTCCGGCAAGATGAAAGAgggggg           >  2:5706962/1‑99 (MQ=18)
                                                                                      cTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CCCGGGGGGG.CG..CCG..CCCC.CCCCCCCCAGCCATTTCGGCCAGGAAGAAAGAggggggggc        >  1:4745001/1‑96 (MQ=9)
                                                                                          gagagaTTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...CGGGG.CT..CCGC.CCCCCCACCCCCGAGGCATTTCAGGCAAGATGAAAGCAGGGCGCTcgcgcg   >  2:3861534/1‑96 (MQ=18)
                                                                                            gagaTTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CCCG.GGGGG.CC..CCC..CCCCGCGCCCCCCCGGGGTTTCCGGCAGAAGGAAAAAGGGGGGCCCGggcgc  >  2:4722940/1‑100 (MQ=2)
                                                                                                  |                                                                                                     
TGGGGGGAACGACGGTCTTCCTGTGTGCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...GCGGG.CT..CCG..CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGG  >  NC_014034/1965141‑1965331

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG