Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,965,271 C→G 100% intergenic (+39/+53) bdhA → / ← RCAP_rcc01818 3‑hydroxybutyrate dehydrogenase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,965,2710C→G100.0% 46.6 16intergenic (+39/+53)bdhA/RCAP_rcc018183‑hydroxybutyrate dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/10):  ref base (0/0):  total (6/10)

GATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...GCGGG..CT..CCG..CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTC  >  NC_014034/1965184‑1965366
                                                                                           |                                                                                                     
gATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCGCCC...GGGGG..CG..CCC..cccc                                                                                     >  2:419049/1‑101 (MQ=38)
 aTCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...GGGGG..GG..CCT..cccccg                                                                                   >  2:1708443/1‑99 (MQ=38)
  tCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...CCGGGC.CT..CCC..cccccc                                                                                   >  2:4682411/1‑99 (MQ=38)
     ccGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...GGGGG..GG..CCG..cgccccccgc                                                                               >  2:3308862/1‑95 (MQ=37)
      cGGAACGACGATTTCTGTGGATGCCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CG...GGGGG..CG..CAG..ccccccggacc                                                                              >  2:939848/1‑94 (MQ=21)
          acgacgATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...GGGGG..GG..CCGC.CCCCCGAccccccg                                                                           >  2:3781504/1‑99 (MQ=25)
              cgaTTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...GGGGG..GTGCCCC..CCCCGGCccccccggga                                                                        >  2:5703908/1‑96 (MQ=21)
                  ttgtgggggtGGCGGGTGGGCGGGGCGGGGAGAGATTCCCCCGCGGCGCAACCCGGGGCCCCCCCG.CC...GGGGG..CT..CCGC.CCCCGGACCCCCGAGGTATTTc                                                                   <  2:4655465/92‑1 (MQ=2)
                                       ggCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...GGGGG..CT..CCGC.CCCCGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGgcg                                              >  2:2864647/1‑101 (MQ=21)
                                       ggCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...CGGGG..GT..CCGCCCCCCGGACCCCC.AGGGATTTCCGGCAAGATGAAAGAgggggg                                              >  2:5706962/1‑99 (MQ=18)
                                           cTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CCCGGGGGGG..CG..CCG..CCCC.CCCCCCCCAGCCATTTCGGCCAGGAAGAAAGAggggggggc                                           >  1:4745001/1‑96 (MQ=9)
                                               gagagaTTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...CGGGG..CT..CCGC.CCCCCCACCCCCGAGGCATTTCAGGCAAGATGAAAGCAGGGCGCTcgcgcg                                      >  2:3861534/1‑96 (MQ=18)
                                               gaaactctccgcCGCGGGACAACCCCGGGCCGCCCCG.CC...GGGGG..CT..CCGC.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAggcg                                      <  1:3086230/92‑1 (MQ=17)
                                                 gagaTTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CCCG.GGGGG..CC..CCC..CCCCGCGCCCCCCCGGGGTTTCCGGCAGAAGGAAAAAGGGGGGCCCGggcgc                                     >  2:4722940/1‑100 (MQ=2)
                                                       ccgcCGCGGGCCACGCGCGGGCCGCCCCG.CC...GGGGG..CT..CCGC.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCcttctt                              <  2:2382752/100‑1 (MQ=18)
                                                        cccggcGGGCCAGGCCCGGGCCCCCCCG.CC...GGGGG..CT..CCGC.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCttcttc                             <  2:3175623/96‑1 (MQ=18)
                                                             gggggCACGCCCGGGCCGCCCCG.CC...GGGGG..CT..CCGC.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTcgccgc                        <  2:655900/100‑1 (MQ=21)
                                                              ggggCAAGCCCGAGGCGACCCG.CC...GGGGGGTCC..CCC..CCCCGGACCCCC.CGGGATTTCAGGCAAGATGGAAAAGGGGCGGCCAGGGGGCCTTCTTCgccgcc                       >  2:655000/1‑101 (MQ=16)
                                                                          gccccccccG.CC...GGGGG..CT..CCGC.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGtc           <  1:714661/95‑1 (MQ=25)
                                                                              gccccG.CC...GGGGG..CT..CCGC.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCgg       <  1:1516874/99‑1 (MQ=255)
                                                                              cccccG.CC...GGGGG..CT..CCGC.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCgg       <  1:2136738/99‑1 (MQ=255)
                                                                              cccccG.CC...GGGGG..CT..CCGC.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCgg       <  1:419093/99‑1 (MQ=255)
                                                                              cccccG.CC...GGGGG..CT..CCGC.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCgg       <  1:4470079/99‑1 (MQ=255)
                                                                               ccccG.CC...GGGGG..CT..CCGC.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCggg      <  1:4105728/100‑1 (MQ=255)
                                                                                 ccg.cc...gGGGG..CT..CCGC.CCCCGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGct    >  2:2748579/1‑101 (MQ=37)
                                                                                  cg.cc...ggGGG..CTC.CCC..CCCCGGACCCCCTAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGctt   >  1:1871959/1‑101 (MQ=255)
                                                                                   g.cc...ggGGG..CT..CCGC.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAACAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGcttc  <  1:1378221/101‑1 (MQ=37)
                                                                                           |                                                                                                     
GATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC...GCGGG..CT..CCG..CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTC  >  NC_014034/1965184‑1965366

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG