Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,976,894 C→G 84.9% intergenic (‑73/+7) fba ← / ← gap1 fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,976,8940C→G84.9% 75.4 ‑0.2 0.0199intergenic (‑73/+7)fba/gap1fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (100/69):  ref base (17/13):  total (117/82)
Fisher's exact test strand distribution p-value = 8.42e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

CCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CGGCGTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT  >  NC_014034/1976801‑1976990
                                                                                                   |                                                                                                
ccGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTtct                                                                                           >  1:5085411/1‑101 (MQ=255)
 cGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTtctc                                                                                          >  2:220280/1‑101 (MQ=255)
  gAAGGGTGATCGGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.GGCG.CCC.CCGGCTTTTCTCa                                                                                         <  2:2766988/101‑1 (MQ=25)
   aaGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAg                                                                                        >  2:2644021/1‑101 (MQ=255)
   aaGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAg                                                                                        >  2:3862154/1‑101 (MQ=255)
    aGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGa                                                                                       >  1:2626941/1‑101 (MQ=255)
     ggtggATCAGGCCCTTTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATCCCCGGGGGGTCG.GGGGGC.AGCC.CCC.CCGGCTTTTCTCAGAt                                                                                      <  2:3262193/97‑1 (MQ=255)
      ggTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCTTTTTTCTTGG.CCG.AAATACGCGGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATc                                                                                     <  2:5557753/101‑1 (MQ=37)
      ggTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATc                                                                                     >  1:105591/1‑101 (MQ=255)
       gTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCa                                                                                    >  2:499126/1‑101 (MQ=255)
        tGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAg                                                                                   >  1:3756687/1‑101 (MQ=255)
           tcggTGCCTTTTTCTCAGTTCCTTGAGTTCGGGGCCTTTTTCTTGG.CCG.AAATCCGCGGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCtt                                                                                <  1:2710406/98‑1 (MQ=25)
           tCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCtt                                                                                >  1:4310832/1‑101 (MQ=255)
            cAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTg                                                                               >  2:3926826/1‑101 (MQ=255)
             atgcccATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGc                                                                              <  2:3521790/97‑1 (MQ=25)
             aGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGc                                                                              >  2:3582587/1‑101 (MQ=255)
             aGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGc                                                                              >  2:843221/1‑101 (MQ=255)
              gTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGcc                                                                             >  1:5645046/1‑101 (MQ=255)
               tGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGccc                                                                            <  2:795565/101‑1 (MQ=255)
               tGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGccc                                                                            <  2:3641383/101‑1 (MQ=255)
               tGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGccc                                                                            >  2:714883/1‑101 (MQ=255)
                gCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCa                                                                           >  2:4065975/1‑101 (MQ=255)
                ccttttttCTAAGTTCTTTGGGTTCGCGGCTTTTTTCTGGG.CCA.AAATCCGCGGGGGGCCG.GGGGGC.AGCC.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCa                                                                           <  2:160989/97‑1 (MQ=255)
                  cttttcCTCAGTCCGTGGAGTCCGCGGCTTTTTTCTGGG.CCG.AAATCCGCGGGGGGCCC.GGGGGC.AGCC.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATc                                                                         <  1:3582804/99‑1 (MQ=255)
                     tttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGcc                                                                      >  1:2773647/1‑101 (MQ=255)
                     tttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGcc                                                                      >  2:1956175/1‑101 (MQ=255)
                       tctcAGTTCTTTGAGTCCGC.GCCTTTTTTTTGGCCCG.AAATCCGCGGGGGGCCG.GGGGGC.AGCC.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAc                                                                    <  2:2296288/101‑1 (MQ=255)
                         tcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATTGCCaacg                                                                  >  2:5313358/1‑98 (MQ=25)
                         tcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcgg                                                                  >  1:5325177/1‑101 (MQ=255)
                          cAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcggc                                                                 >  1:4362577/1‑101 (MQ=255)
                           agatCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcagcg                                                                >  2:4745978/4‑101 (MQ=25)
                           aGTTCCTTGAGTTCGCGGCTTTTTTCTGGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcggcg                                                                <  2:3409705/101‑1 (MQ=37)
                           aGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCCGCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCAcggcg                                                                >  1:4035139/1‑101 (MQ=255)
                           aGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcggcg                                                                >  1:3202076/1‑101 (MQ=255)
                             ttCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGt                                                              >  1:1877053/1‑101 (MQ=255)
                             ttCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGt                                                              >  2:5750223/1‑101 (MQ=255)
                             ttCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGt                                                              >  1:2291534/1‑101 (MQ=255)
                                ctggggtccgcgGCTTTTTTCTGGG.CCG.AAATCCGCGGGGGGTCG.GGGGGC.AGCC.CCC.CGGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATc                                                           <  2:2505448/93‑1 (MQ=21)
                                 ttGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCg                                                          >  2:4524456/1‑101 (MQ=255)
                                   ggggtCGGGGGCTTTTTTTGGG.CCGAAAATCCGCGGGGGGCCG.GGGGGCGGGCC.CCC.CGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCgg                                                         <  1:5698961/97‑1 (MQ=12)
                                   gAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGc                                                        >  2:2343115/1‑101 (MQ=255)
                                    ggTTCGCGCCGTTTTTCGGGC.CCA.AAATCCGCCGGGGGCCC.GGGGCC.GGCC.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGcc                                                       <  1:4330704/100‑1 (MQ=16)
                                      ttCGCGGCTTTTTTTTTGG.CCA.AAAT.CCCCGGGGGTCCGGGGGGC.ACCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCAt                                                     <  1:837048/101‑1 (MQ=21)
                                        cgcgGCCTTTTTTTGGG.CCA.AAATCGGCCGGGGGCCG.GGGGGC.GGCC.CCC.CGGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGc                                                   <  1:3700443/101‑1 (MQ=21)
                                        cgcgGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGc                                                   >  1:615024/1‑101 (MQ=255)
                                           ggCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGt                                                >  1:2247127/1‑101 (MQ=255)
                                           gcccTTTTTTTTGGCCCA.AAACCCCCCGGGGGCCC.GGGGCC.CCCC.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCgg                                                 <  1:2388139/99‑1 (MQ=12)
                                            gCCTTTTTCTTGG.CCG.AAATCCGCGGGGGGTCG.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGtt                                               <  2:3351272/101‑1 (MQ=25)
                                             gcTTTTTCCTGGCCCG.AACTCCGCGGGGGGTCG.GGGGCC.ACCG.CCC.CCGCCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGtt                                               <  2:3320687/100‑1 (MQ=18)
                                             cttttttGTTGG.CCG.AATTCCGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTc                                              <  1:2874942/99‑1 (MQ=25)
                                             ccTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTc                                              <  2:5394031/101‑1 (MQ=255)
                                              cTTTTTCTTGC.CCG.AAATCCGCCGGGGGCCG.GGGGGC.GGCC.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCg                                             <  2:2412038/101‑1 (MQ=21)
                                                ttttCTTGG.CCG.AAATCCGCGGGGGTTCC.GGGGGC.AGCC.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgag                                           <  2:3559130/101‑1 (MQ=25)
                                                ttttCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgag                                           >  2:1200464/1‑101 (MQ=255)
                                                ttttCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgag                                           >  2:5628387/1‑101 (MQ=255)
                                                  ttCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGaa                                         >  1:339261/1‑101 (MQ=255)
                                                  ttCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGaa                                         >  1:4422036/1‑101 (MQ=255)
                                                   tccgatctcG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGaa                                         <  2:2831816/93‑1 (MQ=25)
                                                    cTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCGATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGc                                       >  1:4940999/1‑101 (MQ=25)
                                                     ttGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.CGCG.CCC.CCAGCTTTTCTCAGATCAGCGTGCCCATAGCCACGGCGGTATCGGACATGCGGTTCACGAAGcc                                      >  2:1905242/1‑101 (MQ=25)
                                                       gg.CCG.AAATGCGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcccc                                    <  2:1937317/101‑1 (MQ=25)
                                                       gg.CCG.AAATACGCGGGGGGTCC.GGGGCC.GGCC.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcccc                                    <  1:1607430/101‑1 (MQ=21)
                                                           cG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcatt                                 >  2:5667470/1‑101 (MQ=255)
                                                            g.AAATCCGCGGGGGGCCG.GGGGGC.GGCG.CCCACGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattc                                <  1:972307/101‑1 (MQ=17)
                                                               aaTACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcat                              >  1:3329005/1‑101 (MQ=255)
                                                                  aCGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGt                           >  2:515841/1‑101 (MQ=255)
                                                                   cGCGGGGGGTCC.GGGGGC.CGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTc                          <  1:5440673/101‑1 (MQ=21)
                                                                   cGCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTc                          >  1:2813795/1‑101 (MQ=255)
                                                                    gCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTct                         >  1:1149806/1‑100 (MQ=255)
                                                                    gCCGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCa                         >  2:4450013/1‑101 (MQ=255)
                                                                     cggggggTCC.GGGGGC.AGCC.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCAt                        <  1:5592929/99‑1 (MQ=21)
                                                                     cggggggCCG.GGGGGC.AGCC.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCAt                        <  1:4831822/99‑1 (MQ=18)
                                                                      cGGGGGTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATa                       >  1:5107707/1‑101 (MQ=255)
                                                                       gggggTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAc                      >  1:4681317/1‑101 (MQ=255)
                                                                         gggTCC.GGGGGC.AGCC.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCa                    <  1:1647747/101‑1 (MQ=18)
                                                                           gTCG.GGGGGC.AGCC.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACg                  <  1:2565520/101‑1 (MQ=18)
                                                                           gTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACg                  >  2:2087508/1‑101 (MQ=255)
                                                                           gTCC.GGGGGC.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACg                  >  2:4369655/1‑101 (MQ=255)
                                                                                gggggC.AGCGCCCC.CGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAg              >  2:1664764/1‑101 (MQ=255)
                                                                                gggggC.AGCGCCCC.CGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCAAGACAg              >  2:2280511/1‑101 (MQ=18)
                                                                                 ggggC.AGCGCCCC.CGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAgg             >  1:3518906/1‑101 (MQ=255)
                                                                                 ggggC.AGCGCCCC.CGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAgg             >  1:4908420/1‑101 (MQ=255)
                                                                                 ggggC.AGCGCCCC.CGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAgg             >  2:2951172/1‑101 (MQ=255)
                                                                                  nnnnnnnnnnccc.cGGC.NTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAgg             <  2:1515017/91‑1 (MQ=18)
                                                                                   ggcccccccccc.cGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGa            <  1:2990627/92‑1 (MQ=18)
                                                                                    gggggcccccc.cGGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGa            <  2:3092824/93‑1 (MQ=18)
                                                                                    gcccccccccc.cGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGAt           <  1:5735868/93‑1 (MQ=18)
                                                                                     c.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc         >  2:4478777/1‑101 (MQ=255)
                                                                                     c.AGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc         >  1:670341/1‑101 (MQ=255)
                                                                                      cagcccccc.cGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc         <  2:3754392/95‑1 (MQ=255)
                                                                                       aGCG.CCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCg        <  2:4674479/101‑1 (MQ=255)
                                                                                        gcg.cCC.CCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCgg       >  1:5011320/1‑101 (MQ=255)
                                                                                        gcccccc.cGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCgg       <  1:4524728/97‑1 (MQ=255)
                                                                                          gcccc.cGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAc     >  1:776693/3‑101 (MQ=255)
                                                                                          gcccc.cGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAc     >  1:1763958/3‑101 (MQ=255)
                                                                                           cccc.cGGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAc     <  1:1200545/100‑1 (MQ=255)
                                                                                           cccc.cGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAcc    <  2:3201887/100‑1 (MQ=255)
                                                                                           cccc.cGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAcc    <  2:776553/100‑1 (MQ=255)
                                                                                            ccc.cGGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAcc    <  2:4618566/101‑1 (MQ=255)
                                                                                            ccc.cGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCa   <  1:2097514/101‑1 (MQ=255)
                                                                                                   |                                                                                                
CCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGCCGGGGGTCC.GGGGGC.AGCG.CCC.CGGCGTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT  >  NC_014034/1976801‑1976990

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG