Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,057,834 A→C 14.2% intergenic (‑600/‑302) RCAP_rcc01900 ← / → RCAP_rcc01901 hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,057,8340A→C14.2% 65.1 ‑0.4 ‑0.1176intergenic (‑600/‑302)RCAP_rcc01900/RCAP_rcc01901hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (10/15):  ref base (68/83):  total (78/98)
Fisher's exact test strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.28e-01

Only 100 of 185 total aligned reads displayed.

CTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.GC.G.CGC.AGCG.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAA  >  NC_014034/2057735‑2057911
                                                                                                          |                                                                               
cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC..C.G.CGC.CGc                                                                               <  2:4924707/101‑1 (MQ=255)
 tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC..C.G.CGC.CGca                                                                              <  2:3612525/101‑2 (MQ=255)
 tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC..C.G.CGC.CGca                                                                              >  1:3859925/1‑100 (MQ=255)
  gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC..C.G.CGC.CGcaa                                                                             >  2:4750152/1‑99 (MQ=255)
   tttTCCGCCCATTAGCCCTGCTTCGGCGCGTGGACTTCGGGGGGGCGGCGGGTCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC..C.G.CGC.CGcaag                                                                            <  2:1890280/101‑4 (MQ=255)
   tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC..C.G.CGC.CGcaag                                                                            >  2:2365727/1‑98 (MQ=255)
   tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC..C.G.CGC.CGcaag                                                                            <  1:3647696/101‑4 (MQ=255)
     tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC..C.G.CGC.CGcaagcg                                                                          >  1:2294712/1‑96 (MQ=255)
      aCCGCCCATTAACCCTGCTTAGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC..C.G.CGC.CGccagcgt                                                                         >  2:913055/1‑95 (MQ=255)
        cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC..C.G.CGC.CGcaagcgttt                                                                       <  2:3079999/101‑9 (MQ=255)
         gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC..C.G.CGC.CGcaagcgtttc                                                                      <  2:4923648/101‑10 (MQ=255)
         gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC..C.G.CGC.CGcaagcgtttc                                                                      <  1:1612047/101‑10 (MQ=255)
         gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC..C.G.CGC.CGcaagcgtttc                                                                      >  1:883146/1‑92 (MQ=255)
         gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC..C.G.CGC.CGcaagcgtttc                                                                      <  2:3218194/101‑10 (MQ=255)
          cccATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC..C.G.CGC.CGcaagcgtttcc                                                                     <  1:201301/101‑11 (MQ=255)
          cccATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC..C.G.CGC.CGcaagcgtttcc                                                                     >  2:1555414/1‑91 (MQ=255)
                aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCg                                                                   >  2:1573778/1‑101 (MQ=255)
                aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCg                                                                   >  2:4311025/1‑101 (MQ=255)
                 ttcggctatCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCgg                                                                  <  1:4698755/93‑1 (MQ=255)
                  cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGc                                                                 <  2:4288153/101‑1 (MQ=255)
                   ccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCa                                                                <  1:2719473/101‑1 (MQ=255)
                    cTGCTTCGCCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGCTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCAt                                                               >  2:4814538/1‑101 (MQ=255)
                     tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATc                                                              >  1:3511466/1‑101 (MQ=255)
                      gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCa                                                             <  1:4513587/101‑1 (MQ=255)
                      gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCa                                                             <  1:5110190/101‑1 (MQ=255)
                      gCTTCGGCGCGCGGACTTCGGGGCGGAGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCa                                                             <  1:1491556/101‑1 (MQ=255)
                       cTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCat                                                            <  2:1388783/101‑2 (MQ=255)
                       cTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CCNGC.GCCGCAAGCGTTTCCGAACCGGCATCat                                                            >  1:197244/1‑100 (MQ=255)
                        ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAA.C.CC.GC.GCCGCAAGCGTTTCCGAACCGGCAtaatt                                                           >  1:1156982/1‑97 (MQ=255)
                        ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCatt                                                           <  2:2993693/101‑3 (MQ=255)
                        ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCatt                                                           >  2:4575027/1‑99 (MQ=255)
                        ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCatt                                                           <  2:822817/101‑3 (MQ=255)
                        ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCatt                                                           >  1:696911/1‑99 (MQ=255)
                        ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCatt                                                           <  1:1029305/101‑3 (MQ=255)
                        ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCatt                                                           >  1:1569643/1‑99 (MQ=255)
                         tCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTc                                                          <  2:769837/101‑1 (MQ=255)
                          cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCt                                                         >  2:3745674/1‑101 (MQ=255)
                          cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCt                                                         <  2:3016835/101‑1 (MQ=255)
                           ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCtt                                                        >  1:602327/1‑101 (MQ=255)
                           ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCtt                                                        <  2:658420/101‑1 (MQ=255)
                            gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTg                                                       >  2:5327472/1‑101 (MQ=255)
                            gcgcgcGGACGTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTg                                                       <  2:1103492/101‑1 (MQ=255)
                             cgcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTgg                                                      >  1:2662015/1‑101 (MQ=255)
                             cgcgcgGACTTCGGGGCAGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTgg                                                      <  1:2830365/101‑1 (MQ=255)
                              gcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.c                                                    <  1:2888731/101‑1 (MQ=255)
                                gcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccc                                                  >  1:590430/1‑101 (MQ=255)
                                 cgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cccc                                                 >  1:1285168/1‑101 (MQ=255)
                                 cgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cccc                                                 <  1:5706533/101‑1 (MQ=255)
                                 cgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cccc                                                 >  1:3429876/1‑101 (MQ=255)
                                 ccgACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cccc                                                 <  2:1024579/99‑1 (MQ=255)
                                  ggACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccc                                                <  1:218588/101‑1 (MQ=255)
                                   gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cccccc                                               <  2:4631801/101‑2 (MQ=255)
                                   gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cccccc                                               <  2:1788743/101‑2 (MQ=255)
                                    gcTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccg                                              <  1:4079433/100‑3 (MQ=255)
                                    aCTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccg                                              <  1:4814819/101‑3 (MQ=255)
                                      ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccgcc                                            >  1:5209739/1‑97 (MQ=255)
                                      ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccgcc                                            <  1:1817323/101‑5 (MQ=255)
                                       tCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccgccg                                           <  2:5787656/101‑6 (MQ=255)
                                        cGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCgg                                           <  2:5190275/101‑1 (MQ=255)
                                         ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCggg                                          <  2:1655807/101‑1 (MQ=255)
                                         ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCggg                                          >  1:4182102/1‑101 (MQ=255)
                                          gggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGa                                         >  1:4633340/1‑101 (MQ=255)
                                          gggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGa                                         <  2:5455272/101‑1 (MQ=255)
                                           ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGAc                                        <  1:2148911/101‑1 (MQ=255)
                                           ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGAc                                        <  2:5239790/101‑1 (MQ=255)
                                            gcggcggcgTGGCTCTTTCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACa                                       <  1:936546/101‑1 (MQ=255)
                                             cggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACaa                                      <  2:2927556/101‑1 (MQ=255)
                                              ggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAAt                                     <  2:2631554/101‑1 (MQ=255)
                                                 ggcggGGCTGTTGCCATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                  <  2:372010/101‑1 (MQ=255)
                                                 ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                  >  2:272798/1‑101 (MQ=255)
                                                 ggcggAGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                  >  2:4237504/1‑101 (MQ=255)
                                                   cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACgg                                >  2:717646/1‑101 (MQ=255)
                                                     tctctctTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgcc                              <  2:3588296/98‑1 (MQ=255)
                                                     gggCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgcc                              <  2:3291773/101‑1 (MQ=255)
                                                          ctTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                         >  1:3807999/1‑101 (MQ=255)
                                                           ttGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCa                        <  2:3465290/101‑1 (MQ=255)
                                                            tGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                       <  1:2337936/101‑1 (MQ=255)
                                                            tGCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                       >  1:4763432/1‑101 (MQ=255)
                                                             gCGATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAtt                      <  2:5703768/101‑1 (MQ=255)
                                                               gATCCTGCGTTTCCGGATTGGCGAC.AA.TCCC.GCTC.CGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGt                    <  2:1480403/101‑1 (MQ=255)
                                                                  ccTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAGCCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                 <  1:659775/101‑1 (MQ=255)
                                                                  ccTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                 <  2:2352752/101‑1 (MQ=255)
                                                                  ccTGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                 <  1:80029/101‑1 (MQ=255)
                                                                    tGCGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGc               >  1:5375591/1‑101 (MQ=255)
                                                                      cGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCa             >  2:1178539/1‑101 (MQ=255)
                                                                      cGTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCa             <  2:3511249/101‑1 (MQ=255)
                                                                       gTTTCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt            >  1:3038524/1‑101 (MQ=255)
                                                                        tttCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGGCTCATTGTTCCGCCAtt           <  1:2440459/101‑1 (MQ=255)
                                                                         ttCCGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg          <  2:3910792/101‑1 (MQ=255)
                                                                          tCCGTATTGCCCAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcaagctg         <  2:4003679/101‑7 (MQ=255)
                                                                           ccGGATTGGCCAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCTGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCg        <  1:4237764/101‑1 (MQ=255)
                                                                            cGGATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGa       >  1:3907494/1‑101 (MQ=255)
                                                                             ggATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCgct      >  2:5764902/1‑99 (MQ=255)
                                                                             ggATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt      >  2:129953/1‑101 (MQ=255)
                                                                             ggATTGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt      >  1:328837/1‑101 (MQ=255)
                                                                              gATTGGCGAC.AACC.CC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc     <  1:5284088/101‑1 (MQ=255)
                                                                                ttGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa   >  2:1282623/1‑101 (MQ=255)
                                                                                 tGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa  >  2:2251762/1‑101 (MQ=255)
                                                                                 tGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa  <  1:1896036/101‑1 (MQ=255)
                                                                                 tGGCGAC.AA.TCCC.GC.GCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa  <  2:2823660/101‑1 (MQ=255)
                                                                                                          |                                                                               
CTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGAC.AA.T.CC.GC.G.CGC.AGCG.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAA  >  NC_014034/2057735‑2057911

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG