BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,155,440	G→C	11.9%	S2T (AGC→ACC)	RCAP_rcc02000 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,155,440	0	G→C	11.9%	73.8 ‑1.4 ‑1.3	261	S2T (AGC→ACC)	RCAP_rcc02000	hypothetical protein
Reads supporting (aligned to +/- strand): new base (19/12): ref base (112/118): total (131/130)
Fisher's exact test strand distribution p-value = 2.51e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.42e-02

Only 100 of 274 total aligned reads displayed.


CCGCCGACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACC  >  NC_014034/2155340‑2155540


  gccgACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCg                                                                                                    >  1:36061/1‑101 (MQ=255)


   ccgCCCCGGCCGCGGTCCCCCTCCCCTTCTGATGCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGa                                                                                                   <  1:1912600/101‑1 (MQ=255)


   ccgCCCCGGCCCCGGTCCCCCCCCCCTTCGGATGCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGa                                                                                                   <  1:2556258/101‑1 (MQ=255)


    cgccccGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGAt                                                                                                  <  2:431538/98‑1 (MQ=255)


    cgACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGAt                                                                                                  >  2:2138880/1‑101 (MQ=255)


     gccccGGCCGCGGTCCCCCTCACCTTCTGATCCCCTCGGGCCCCGTCCCCGTCACCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATg                                                                                                 <  2:813269/99‑1 (MQ=255)


      ccccGGCCCCGGTCGCCCCCCCCTTCCGGTGCCCTGGGGGGGCCTCCCCGTCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGc                                                                                                <  1:4344801/100‑1 (MQ=255)


       cccGGCCGCGGTCGCCCTCACCTTCTGATCCCCTCGGGCGCCGTCACCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                               <  1:2139002/101‑1 (MQ=255)


         cGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAg                                                                                             >  1:2835257/1‑101 (MQ=255)


                ggTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                      >  1:2857351/1‑101 (MQ=255)


                ggTCCCCCTCCCCTTCGGATGCCCCCGGGCGCCGTCCCCTTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                      <  1:4781213/101‑1 (MQ=255)


                 gTCCCCCTCCCCTTCTGATCCCCTGGGGCGCGGCCCCCGTCCCCGCCCGCGGGGCGCCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                     <  2:2430958/101‑1 (MQ=255)


                  tccccccccccTTCTGACCCCCTCGGGCGCCGCCCCCCCCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGt                                                                                    <  1:4030443/92‑1 (MQ=255)


                  tcccccTCCCTTTTTGATCCCCTGGGCCGCCGCCCCCGCCCCCCCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGt                                                                                    <  2:222539/98‑1 (MQ=255)


                   cGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTa                                                                                   >  2:5631098/1‑101 (MQ=255)


                    gCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                  >  1:1048373/1‑101 (MQ=255)


                    gCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                  >  2:327696/1‑101 (MQ=255)


                    gCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                  >  2:5738155/1‑101 (MQ=255)


                     cccTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTaga                                                                                 >  1:4398239/1‑101 (MQ=255)


                      ccTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                >  2:3001509/1‑101 (MQ=255)


                      ccTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                >  1:419684/1‑101 (MQ=255)


                      ccTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                >  1:3913340/1‑101 (MQ=255)


                       cTCCCCTTCTGACGCCCCGGGGGGCCGTCCCCGTCCCCCCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGc                                                                               <  1:1798328/101‑1 (MQ=255)


                          aCCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcg                                                                            >  1:1176997/1‑101 (MQ=255)


                            cTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtc                                                                          >  1:854944/1‑101 (MQ=255)


                                tGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATg                                                                      >  1:3048827/1‑101 (MQ=255)


                                  aTGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGcc                                                                    >  1:799319/1‑101 (MQ=255)


                                   cccccTGGGGCGCCACCCCCGCCCCCCCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCa                                                                   <  2:886227/99‑1 (MQ=255)


                                   cccccGCGGGCGCCGCCCCCGCCCCCGCCCGCGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCa                                                                   <  2:4515055/99‑1 (MQ=255)


                                    gCCCTGGGGCGCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCAt                                                                  <  2:54073/101‑1 (MQ=255)


                                       cgcGGGCCCCGTCCCCGCCCCCCCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTg                                                               <  1:2898958/99‑1 (MQ=255)


                                         ggggCGCCGTCCCCCTCCCCGCCCTCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggg                                                             <  1:2318320/100‑1 (MQ=255)


                                         cGGGCCCCCTCCCCGCCCCCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggg                                                             <  2:677451/101‑1 (MQ=255)


                                          gggCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgggg                                                            >  2:1980479/1‑101 (MQ=255)


                                            gcgcCGCCCCCGCCACCCCCCGCGGGGGGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcg                                                          <  2:5525374/101‑1 (MQ=255)


                                             ccccgtccccgtccCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcgg                                                         <  2:139327/99‑1 (MQ=255)


                                              gcCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcggg                                                        >  1:347139/1‑101 (MQ=255)


                                              gcCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcggg                                                        >  1:5672741/1‑101 (MQ=255)


                                                 gtcaccgtcaccGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                     >  2:4174628/1‑101 (MQ=255)


                                                 gcccccgcccccgcccgcGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                     <  1:4548781/91‑1 (MQ=255)


                                                    cccgcgcccccccgccGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCTTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTc                                                  <  2:50783/91‑1 (MQ=255)


                                                    cccgcccccgcccgcGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTc                                                  <  1:2672708/94‑1 (MQ=255)


                                                      cgcccccgcccgcCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcac                                                <  1:3858703/96‑1 (MQ=255)


                                                       gtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                               >  1:3681781/1‑101 (MQ=255)


                                                          ccccccggcggggCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACg                                            <  1:2386181/91‑1 (MQ=255)


                                                           ccgcccgcCGGGCGGCAAGTCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGa                                           >  2:388636/1‑101 (MQ=255)


                                                              cccgcgGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATgg                                        <  1:1955556/101‑1 (MQ=255)


                                                              cccgccGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATgg                                        <  2:3940410/101‑1 (MQ=255)


                                                                cgcggGGCGCAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcg                                      <  1:3984740/101‑1 (MQ=255)


                                                                cgccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcg                                      >  1:1582054/1‑101 (MQ=255)


                                                                 gccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcgg                                     >  2:2748424/1‑101 (MQ=255)


                                                                  cggggGGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggc                                    <  2:4601317/99‑1 (MQ=255)


                                                                   cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                   >  1:1093976/1‑101 (MQ=255)


                                                                    gggCGGCAAGCCGCGAAAGGGCAGGAGCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCAAGATggcggcgg                                  >  1:4418907/1‑101 (MQ=255)


                                                                     ggcggcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcggc                                 >  2:4436502/1‑101 (MQ=255)


                                                                      gggaaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGgcggcggcg                                <  1:328307/96‑1 (MQ=255)


                                                                       cggcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGGCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGgcgc                               >  2:2952558/1‑101 (MQ=255)


                                                                        gcaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCt                              <  1:4469446/98‑1 (MQ=255)


                                                                         gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                             >  2:5242817/1‑101 (MQ=255)


                                                                         gaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCCCCACGATGGCGGCGGCGCTc                             <  2:2557419/99‑1 (MQ=255)


                                                                         gaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                             <  2:3383862/99‑1 (MQ=255)


                                                                         gaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                             <  2:2886864/99‑1 (MQ=255)


                                                                            aGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGcc                          <  1:2988201/101‑1 (MQ=255)


                                                                               cgcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCggg                       <  1:5738450/101‑1 (MQ=255)


                                                                                gcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggc                      <  1:662507/101‑1 (MQ=255)


                                                                                 cgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcg                     >  2:5582987/1‑101 (MQ=255)


                                                                                  gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                    >  2:1583753/1‑101 (MQ=255)


                                                                                   aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                   >  1:1449685/1‑101 (MQ=255)


                                                                                   aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGgccggc                   >  2:3179812/1‑101 (MQ=255)


                                                                                    aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                  <  1:4917003/101‑1 (MQ=255)


                                                                                     aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTg                 <  2:1737288/101‑1 (MQ=255)


                                                                                      cgacaaaaaaaTGACCGATACCAGATGCCTGGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGa                <  2:1651560/91‑1 (MQ=255)


                                                                                       ggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAt               >  2:1301009/1‑101 (MQ=255)


                                                                                        gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg              >  2:1439536/1‑101 (MQ=255)


                                                                                        agaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtg              <  1:5024943/93‑1 (MQ=255)


                                                                                        agaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtg              >  2:3786749/9‑101 (MQ=255)


                                                                                         gaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgt             <  1:5348430/94‑1 (MQ=255)


                                                                                         cAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgt             >  1:2784394/1‑101 (MQ=255)


                                                                                          aaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgtg            <  2:3621215/95‑1 (MQ=255)


                                                                                          aaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgtg            <  1:1226739/95‑1 (MQ=255)


                                                                                          aaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgtg            >  1:4657438/7‑101 (MQ=255)


                                                                                          aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg            <  2:2665210/101‑1 (MQ=255)


                                                                                           aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCAGCACGACGATGGCGGCGGCGCAGGCCGGGCGCCTGATGTgg           >  1:5698073/6‑101 (MQ=255)


                                                                                            gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc          >  1:1347014/1‑101 (MQ=255)


                                                                                             aaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCa         <  2:3013368/98‑1 (MQ=255)


                                                                                             aTCATGAGCGATGCAAGGGGCCTCATAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa         <  1:4564500/101‑1 (MQ=255)


                                                                                               cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt       <  2:543221/101‑1 (MQ=255)


                                                                                               agtGAGCGATGCATGGGGCGTGGTAGGCATAGCCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt       <  2:4077211/99‑1 (MQ=255)


                                                                                                aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCGATCTGGGGCGCGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc      <  2:2493565/101‑1 (MQ=255)


                                                                                                aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc      <  1:5121486/101‑1 (MQ=255)


                                                                                                 tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCCTTCg     >  1:2522346/1‑101 (MQ=255)


                                                                                                 tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg     >  1:302778/1‑101 (MQ=255)


                                                                                                 tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGGGGCATTCg     >  2:2773763/1‑101 (MQ=255)


                                                                                                  gAGGGATGCAAGGGGCCTCGTAGAGCTCCTCGATGTCATCTGGGGCGGGGCCGTCGCCACGATGGCGTAGGGGCTCGCCGGGCGGCTGATGTGGCATTCGa    <  2:5757773/101‑1 (MQ=255)


                                                                                                  gAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGa    <  1:5631390/101‑1 (MQ=255)


                                                                                                  gAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGa    >  1:1268072/1‑101 (MQ=255)


                                                                                                   aGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAc   <  1:5386392/101‑1 (MQ=255)


                                                                                                   aGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAc   <  1:5238053/101‑1 (MQ=255)


                                                                                                   aGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAc   <  2:1048298/101‑1 (MQ=255)


                                                                                                    gCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAcc  <  1:2174003/101‑1 (MQ=255)


CCGCCGACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACC  >  NC_014034/2155340‑2155540

Base quality scores:


ATCG

< 3 ≤


ATCG

< 22 ≤


ATCG

< 30 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG