Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,660 C→G 34.3% T75T (ACC→ACG)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,6600C→G34.3% 0.0 ‑3.8 0.0378T75T (ACC→ACG) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (81/49):  ref base (123/125):  total (204/174)
Fisher's exact test strand distribution p-value = 2.24e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 402 total aligned reads displayed.

GCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATG  >  NC_014034/2155560‑2155760
                                                                                                    |                                                                                                    
gccgcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGAcc                                                                                                      <  2:4166776/101‑1 (MQ=255)
  cgcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGt                                                                                                    >  1:1952739/1‑101 (MQ=255)
     ttCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGac                                                                                                 <  2:129203/101‑1 (MQ=255)
       ccTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGacca                                                                                               <  1:3007994/101‑1 (MQ=255)
        cTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGaccac                                                                                              >  1:3463649/1‑101 (MQ=255)
        cTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGaccac                                                                                              <  2:1093901/101‑1 (MQ=255)
         tGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGaccacc                                                                                             <  1:5160612/101‑1 (MQ=255)
         tGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGaccacc                                                                                             >  1:1444915/1‑101 (MQ=255)
           gggcgtgaactgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCgg                                                                                           >  1:5300421/10‑101 (MQ=255)
             ccGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGcg                                                                                         <  2:2001815/101‑1 (MQ=255)
               gcgAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGcgtc                                                                                       >  1:3960132/1‑101 (MQ=255)
                     tgctCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCa                                                                                 <  1:2285489/101‑1 (MQ=255)
                     tgctCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCa                                                                                 >  1:1340102/1‑101 (MQ=255)
                       ctctGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAAc                                                                               <  1:280825/101‑1 (MQ=255)
                        tgtGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGcctcg                                                                              >  2:1036087/3‑97 (MQ=255)
                        tctGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACg                                                                              <  1:1754185/101‑1 (MQ=255)
                          tGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCt                                                                            <  2:1625499/101‑1 (MQ=255)
                           gggAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTc                                                                           <  1:5448463/101‑1 (MQ=255)
                             naGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGAACGTGACCACCGGCGTCGTCGACANNCNCgn                                                                         >  1:1028621/2‑100 (MQ=255)
                             gAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGc                                                                         >  1:4060073/1‑101 (MQ=255)
                               gCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCt                                                                       >  1:5211990/1‑101 (MQ=255)
                               gCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCt                                                                       >  1:825264/1‑101 (MQ=255)
                               gCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCt                                                                       <  2:1937092/101‑1 (MQ=255)
                                 tGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTac                                                                     >  2:1537626/1‑100 (MQ=255)
                                 tGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTac                                                                     >  1:3986071/1‑100 (MQ=255)
                                 tGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAt                                                                     >  2:760709/1‑101 (MQ=255)
                                  gCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAtc                                                                    >  2:4048730/1‑101 (MQ=255)
                                  gCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAtc                                                                    >  1:3860360/1‑101 (MQ=255)
                                  gCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAtc                                                                    <  2:3678790/101‑1 (MQ=255)
                                  gCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAtc                                                                    <  1:611019/101‑1 (MQ=255)
                                  ccccGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAtc                                                                    >  1:5754892/2‑101 (MQ=255)
                                    ccGTCGCGGTCGGCTTGGCGATCATCGGCTAGGTGGTGGCCGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAtctc                                                                  <  2:3411120/101‑1 (MQ=255)
                                     ggtggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCg                                                                 >  1:951366/11‑101 (MQ=255)
                                     cGTCGCGGTCGGCGTGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTTGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCg                                                                 <  2:2849016/101‑1 (MQ=255)
                                      gtggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCgg                                                                >  2:5662375/10‑101 (MQ=255)
                                      gTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCgg                                                                >  1:374985/1‑101 (MQ=255)
                                       tCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGc                                                               <  1:1636733/101‑1 (MQ=255)
                                        cgcgGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGcc                                                              <  2:4391947/101‑1 (MQ=255)
                                          cgGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCcg                                                            >  1:2103345/1‑101 (MQ=255)
                                          ccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcg                                                            >  2:1189407/6‑101 (MQ=255)
                                           cgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgc                                                           >  2:5761910/5‑101 (MQ=255)
                                            gtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgcg                                                          >  1:895199/4‑101 (MQ=255)
                                            gTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCcgcg                                                          >  2:1395768/1‑101 (MQ=255)
                                             tgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgcgc                                                         >  1:3393424/3‑101 (MQ=255)
                                               ggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCgcgg                                                       <  2:799694/101‑1 (MQ=255)
                                               ggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCgcgg                                                       <  2:4427385/101‑1 (MQ=255)
                                                gCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCgcggg                                                      <  1:258623/101‑2 (MQ=255)
                                                gCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCgcggc                                                      >  1:5326105/1‑101 (MQ=255)
                                                gCATGGCGATCAACGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCgcggc                                                      >  1:3126593/1‑101 (MQ=255)
                                                 cATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCgcgggg                                                     <  1:2493588/101‑3 (MQ=255)
                                                  aTGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGgggc                                                    >  2:3828878/1‑101 (MQ=255)
                                                  aTGGCGATCATCGGCGAGGCGGTGGCGGCTTATGTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGgggc                                                    <  1:4979722/101‑1 (MQ=255)
                                                   tGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGcc                                                   <  2:5110103/101‑1 (MQ=255)
                                                    ggCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGccc                                                  <  2:2172746/101‑1 (MQ=255)
                                                     gCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCa                                                 >  1:3018951/1‑101 (MQ=255)
                                                      cGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAg                                                >  2:2652618/1‑101 (MQ=255)
                                                      cGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAg                                                <  1:4436774/101‑1 (MQ=255)
                                                       gATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAgg                                               >  2:4983211/1‑101 (MQ=255)
                                                         tcatcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTg                                             <  2:3023847/101‑1 (MQ=255)
                                                            tcGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctg                                          <  2:4621932/101‑4 (MQ=255)
                                                            agggCGAGGCGGTGGCGGCTTTTTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCGCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctg                                          <  2:3731385/99‑4 (MQ=255)
                                                              ggCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctgct                                        >  2:4673390/1‑96 (MQ=255)
                                                              ggCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCt                                        <  2:4252956/101‑1 (MQ=255)
                                                              ggCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCt                                        >  1:3528481/1‑101 (MQ=255)
                                                              ggCGAGGCGGTGGCGGCCTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCt                                        <  2:5194728/101‑1 (MQ=255)
                                                               gCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAggggctgctg                                       >  1:4310864/1‑93 (MQ=255)
                                                                 gAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAc                                     <  2:3464769/101‑1 (MQ=255)
                                                                 gAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTCAc                                     >  1:3673897/1‑101 (MQ=255)
                                                                  aGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatg                                    >  2:5265848/1‑99 (MQ=255)
                                                                  aGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAcc                                    >  2:2578046/1‑101 (MQ=255)
                                                                  aGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAcc                                    <  1:1109051/101‑1 (MQ=255)
                                                                   ggCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAccc                                   <  1:2763854/101‑1 (MQ=255)
                                                                     cGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgagg                                 <  2:4809829/101‑6 (MQ=255)
                                                                     cGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCgg                                 <  2:5417933/101‑1 (MQ=255)
                                                                       gTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGt                               <  2:3580311/101‑1 (MQ=255)
                                                                       gTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGt                               <  1:5675452/101‑1 (MQ=255)
                                                                        tGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgaggatc                              >  2:4893971/1‑93 (MQ=255)
                                                                         ggcggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtct                             <  2:3232130/101‑1 (MQ=255)
                                                                         ggcggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtct                             >  1:1529406/1‑101 (MQ=255)
                                                                         ggcggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtct                             >  2:5557073/1‑101 (MQ=255)
                                                                            ggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtcttct                          <  2:3089202/101‑1 (MQ=255)
                                                                             gcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTcttctt                         <  2:4470741/101‑1 (MQ=255)
                                                                              cTTATTTCGAGCTCGGCCGGGCCGTGCCCCCCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCttt                        <  1:2731419/101‑1 (MQ=255)
                                                                                tATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTca                      >  1:4712471/1‑101 (MQ=255)
                                                                                tATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTca                      >  2:2261125/1‑101 (MQ=255)
                                                                                  tttCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTcaca                    <  2:298879/101‑1 (MQ=255)
                                                                                    tCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaac                  <  2:2331794/101‑1 (MQ=255)
                                                                                     cGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGCCTTCTTTCacaaca                 >  1:4786716/1‑101 (MQ=255)
                                                                                        gcccGGCCGGACCGTGACCTCCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTCGCGCTGACCCGGGTCTTCTTTCACAACAAgg              <  1:3264058/98‑1 (MQ=255)
                                                                                         cTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGa             >  2:1873346/1‑101 (MQ=255)
                                                                                         cTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGCGGCGCTGACCCGGGCCTTCTTTCACAACAAGGa             >  1:2403607/1‑101 (MQ=255)
                                                                                           cGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGt           >  1:691631/1‑101 (MQ=255)
                                                                                           cGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGt           <  1:4253716/101‑1 (MQ=255)
                                                                                           cGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGt           >  2:1812762/1‑101 (MQ=255)
                                                                                            ggCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTc          >  2:39019/1‑101 (MQ=255)
                                                                                                ggACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCCGGTGGCGCTGACCCAGGTCTTCTTTCACAACAAGGAGGCGtca      >  1:506995/1‑99 (MQ=255)
                                                                                                ggACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTga      <  1:5592142/101‑1 (MQ=255)
                                                                                                 gACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTgag     <  1:1846131/101‑1 (MQ=255)
                                                                                                  aCCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCGGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTgaga    >  1:4869276/1‑101 (MQ=255)
                                                                                                    cGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATg  <  1:4573885/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATG  >  NC_014034/2155560‑2155760

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG