Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,712 C→G 12.4% Q93E (CAG→GAG)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,7120C→G12.4% 81.2 ‑3.0 ‑0.0295Q93E (CAG→GAG) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (23/14):  ref base (113/145):  total (136/159)
Fisher's exact test strand distribution p-value = 5.13e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.92e-01

Only 100 of 317 total aligned reads displayed.

GGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.CCGC.GCAGCTGCGGCTCCGGGCGCTCGGCTTTGAGC  >  NC_014034/2155612‑2155812
                                                                                                    |                                                                                                      
ggCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGccc                                                                                                        <  2:2172746/101‑1 (MQ=255)
  cGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAg                                                                                                      >  2:5675157/1‑101 (MQ=255)
  cGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAg                                                                                                      <  1:2222164/101‑1 (MQ=255)
     tcatcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTg                                                                                                   <  1:3180001/101‑1 (MQ=255)
     tcatcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTg                                                                                                   <  2:3023847/101‑1 (MQ=255)
        agggCGAGGCGGTGGCGGCTTTTTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCGCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctg                                                                                                <  2:3731385/99‑4 (MQ=255)
          ggCGGGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCt                                                                                              <  2:2012505/101‑1 (MQ=255)
          ggCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctgct                                                                                              >  2:4872556/1‑96 (MQ=255)
          ggCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCt                                                                                              >  1:3528481/1‑101 (MQ=255)
          ggCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCt                                                                                              >  1:3490106/1‑101 (MQ=255)
          ggCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCt                                                                                              <  1:5510170/101‑1 (MQ=255)
          ggCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCt                                                                                              <  2:4252956/101‑1 (MQ=255)
          ggCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCt                                                                                              <  2:3463432/101‑1 (MQ=255)
          ggCGAGGCGGTGGCGGCCTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCt                                                                                              <  2:5194728/101‑1 (MQ=255)
           gCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAggggctgctg                                                                                             >  1:4310864/1‑93 (MQ=255)
           gCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTg                                                                                             <  2:3152931/101‑1 (MQ=255)
            cGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGa                                                                                            >  2:5106387/1‑101 (MQ=255)
             gAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGat                                                                                           >  2:2926435/1‑100 (MQ=255)
             gAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGat                                                                                           >  2:2624222/1‑100 (MQ=255)
             gAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGat                                                                                           >  1:1957334/1‑100 (MQ=255)
             gAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGat                                                                                           >  1:869434/1‑100 (MQ=255)
             gAGGCGGTGGCGGCTTATTTCGAGCTCGGCGGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAc                                                                                           <  2:1918371/101‑1 (MQ=255)
             gAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAc                                                                                           >  1:5681378/1‑101 (MQ=255)
             gAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTCAc                                                                                           >  1:3673897/1‑101 (MQ=255)
              aGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAcc                                                                                          >  1:5418223/1‑101 (MQ=255)
               ggCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatga                                                                                         <  1:4760119/101‑4 (MQ=255)
               ggCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAccc                                                                                         <  1:388680/101‑1 (MQ=255)
               ggCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAccc                                                                                         <  1:2763854/101‑1 (MQ=255)
               ggCAGTGGCGGCTTATTTCGGGCTGGGGCGGCCGTTAACCACCGGCGTCGTCGCCGCGCTCGCCTTCCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatga                                                                                         <  2:1145829/101‑4 (MQ=255)
                gCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCg                                                                                        <  2:4418640/101‑1 (MQ=255)
                 cGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgagg                                                                                       <  2:4809829/101‑6 (MQ=255)
                 cGGTGCCGGCTTATTCCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACTCgg                                                                                       <  2:407479/101‑1 (MQ=255)
                  ggTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgagga                                                                                      >  1:5665144/1‑95 (MQ=255)
                    tGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtc                                                                                    >  2:3345939/1‑101 (MQ=255)
                    tGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtc                                                                                    <  2:1323322/101‑1 (MQ=255)
                     ggcggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtct                                                                                   >  2:1505087/1‑101 (MQ=255)
                        ggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtcttct                                                                                <  2:1089036/101‑1 (MQ=255)
                        ggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtcttct                                                                                <  2:3089202/101‑1 (MQ=255)
                        ggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtcttct                                                                                >  2:88273/1‑101 (MQ=255)
                         gcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCCCTGACCCGGGCcttctt                                                                               >  1:1614256/1‑101 (MQ=255)
                          cTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCttt                                                                              <  2:374941/101‑1 (MQ=255)
                             aTTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTcac                                                                           <  1:3984662/101‑1 (MQ=255)
                               ttCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTcacac                                                                         >  2:1940299/1‑100 (MQ=255)
                                tCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaac                                                                        <  2:2331794/101‑1 (MQ=255)
                                 cGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaaca                                                                       >  1:3328358/1‑101 (MQ=255)
                                  gAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCAcaacaa                                                                      <  1:918425/101‑1 (MQ=255)
                                   aGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCCCGCCTATCTCGGCCCGCGCGGCCCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAg                                                                     <  1:96410/101‑1 (MQ=255)
                                     cTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGa                                                                   <  2:4016766/101‑1 (MQ=255)
                                     cTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGa                                                                   <  1:5289981/101‑1 (MQ=255)
                                     cTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGCGGCGCTGACCCGGGCCTTCTTTCACAACAAGGa                                                                   >  1:2403607/1‑101 (MQ=255)
                                      tCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAg                                                                  >  1:3060925/1‑101 (MQ=255)
                                      tCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAg                                                                  >  1:1798314/1‑101 (MQ=255)
                                       cGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGt                                                                 <  1:4253716/101‑1 (MQ=255)
                                       cGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGt                                                                 >  2:1812762/1‑101 (MQ=255)
                                       cGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGt                                                                 >  1:691631/1‑101 (MQ=255)
                                        ggCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTc                                                                >  2:39019/1‑101 (MQ=255)
                                        ggCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTc                                                                <  1:4833011/101‑1 (MQ=255)
                                        ggCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTc                                                                <  1:520561/101‑1 (MQ=255)
                                           cGGCCCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTg                                                             <  2:1421805/101‑1 (MQ=255)
                                            ggACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCCGGTGGCGCTGACCCAGGTCTTCTTTCACAACAAGGAGGCGtca                                                            >  1:506995/1‑99 (MQ=255)
                                             gACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTgag                                                           >  1:591183/1‑101 (MQ=255)
                                             gACCGGGACCACCGGCGTCGGCGCAACGCTCCCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTgag                                                           <  1:2623182/101‑1 (MQ=255)
                                              aCCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCGGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTgaga                                                          >  1:4869276/1‑101 (MQ=255)
                                                cGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATg                                                        <  1:2581210/101‑1 (MQ=255)
                                                cGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATg                                                        >  2:4634097/1‑101 (MQ=255)
                                                cGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATg                                                        <  2:4712190/101‑1 (MQ=255)
                                                cGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATg                                                        <  1:1889158/101‑1 (MQ=255)
                                                    accaccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgacga                                                    <  2:4994106/101‑1 (MQ=255)
                                                     ccaccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgacgac                                                   <  2:801647/101‑1 (MQ=255)
                                                       cccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAg                                                 <  2:3854615/100‑1 (MQ=255)
                                                         cGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGAc                                               >  1:3801652/1‑101 (MQ=255)
                                                            cgtcgtCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTTAGATGACGACAGACTgg                                            <  2:4619269/101‑1 (MQ=255)
                                                            cgtcgtCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTgg                                            <  1:4084325/101‑1 (MQ=255)
                                                             gtcgtcGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGc                                           >  2:5544429/1‑101 (MQ=255)
                                                             gtcgtcGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGc                                           <  2:5246634/101‑1 (MQ=255)
                                                              tcgtcgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCg                                          >  2:2679176/1‑101 (MQ=255)
                                                                   gCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.Gcg                                    >  1:4466367/1‑101 (MQ=255)
                                                                     caCGCTCGCCCATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.Ccgc.g                                 <  2:1614162/100‑1 (MQ=255)
                                                                      aCGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.CCgc.gc                                <  1:1505180/101‑1 (MQ=255)
                                                                        gCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCggggggggccg                                >  1:1037221/1‑93 (MQ=255)
                                                                        gCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.GCgc.gccg                              >  1:2034511/1‑99 (MQ=255)
                                                                        gCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.GCgc.gccg                              >  1:3391675/1‑99 (MQ=255)
                                                                          tCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGCCTTCTTTCACAACAAGGAGTCGTGAGATGACGAAAGACTGGCGGG.Ccgc.ggggcg                            >  1:5555358/1‑96 (MQ=255)
                                                                            gCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.Gcggcgcgcccc                           >  2:672068/1‑92 (MQ=255)
                                                                                  ctcGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGTTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.CCGC.GCAGCTGCGGCTcc                    <  2:3673669/101‑1 (MQ=255)
                                                                                    cGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.CCGC.GCAGCTGCGGCTCCgg                  <  1:1282504/101‑1 (MQ=255)
                                                                                      gCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.CCGC.GCAGCTGCGGCTCCGGgc                <  2:5319642/101‑1 (MQ=255)
                                                                                        ccGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGTCGGG.GAAGCGGCGCCTCCggggc               >  2:2488088/1‑99 (MQ=255)
                                                                                         cgcgcgGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.GCGC.GCCGCGGCGGCGCCGGgggcc             >  1:5642547/1‑100 (MQ=255)
                                                                                         cgcgcgGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.CCGC.GCAGCTGCGGCTCCGGGCGCt             <  1:4634373/101‑1 (MQ=255)
                                                                                           cgcgGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.CCGC.GCAGCTGCGGCTCCGGGCGCTCg           <  1:4435086/101‑1 (MQ=255)
                                                                                             cggcgCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.GCGC.GCGGCTGCGGGTCCCGgcgcgccgc         >  1:3930835/1‑96 (MQ=255)
                                                                                               gcgcCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.CCGC.GCAGCTGCGGCTCCGGGCGCTCGGCtt       <  2:3489889/101‑1 (MQ=255)
                                                                                                cgcCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.CCGC.GCAGCTGCGGCTCCGGGCGCTCGGCttt      <  1:1086100/101‑1 (MQ=255)
                                                                                                cgcCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.CCGC.GCAGCTGCGGCTCCGGGCGCTCGGCttt      <  2:4562412/101‑1 (MQ=255)
                                                                                                cgcCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.CCGC.GCAGCTGCGGCTCCGGGCGCTCGGCttt      <  1:2355215/101‑1 (MQ=255)
                                                                                                 gcCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.CCGC.GCAGCTGCGGCTCCGGGCGCTCGGCTTTg     <  2:1205914/101‑1 (MQ=255)
                                                                                                 gcCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.CCGC.GCAGCTGCGGCTCCGGGCGCTCGGCTTTg     <  2:5754597/101‑1 (MQ=255)
                                                                                                  cccAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.CCGC.GCAGCTGCGGCTCCGGGCGCTCGGCTTTGa    <  1:2992177/101‑1 (MQ=255)
                                                                                                   ccAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.GCGCGGCCGC.CCGGCTCCCGGCGCCCGGcgtgttg   >  1:5737463/1‑95 (MQ=255)
                                                                                                    |                                                                                                      
GGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGG.CCGC.GCAGCTGCGGCTCCGGGCGCTCGGCTTTGAGC  >  NC_014034/2155612‑2155812

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG