Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,186,002	G→C	100%	intergenic (+275/‑230)	mtlK → / → RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,186,002	0	G→C	100.0%	137.8	39	intergenic (+275/‑230)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (23/16):  ref base (0/0):  total (23/16)

AACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACC  >  NC_014034/2185919‑2186099                                                                                       |                                                                                                  accgcgcaaCCGCCCCCTTTCCCCTTGGCTCAAATATCCGGGGGGTCCGGGGGCCGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAccc                                                                                   <  2:696448/94‑1 (MQ=2)         accgcccccTTTCCCCTGGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCa                                                                           <  1:1668203/96‑1 (MQ=0)                        cTTGGCTCAAATATCCCGGGGGCCGGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGa                                                            <  2:2492220/101‑1 (MQ=9)                                                     gcggagcccccGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggc                               <  2:1238457/93‑1 (MQ=21)                                                           cccccGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACg                         <  1:2578370/99‑1 (MQ=25)                                                             cccGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGc                       <  1:4308924/101‑1 (MQ=25)                                                                          gcctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGAGCAAAAGCCCGGCCCGGCAGGACGGCTATGCCTTGAc            >  1:4035153/11‑101 (MQ=255)                                                                           cctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTTCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcc           >  1:3556549/10‑101 (MQ=255)                                                                           cctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcc           >  1:5263791/10‑101 (MQ=255)                                                                           cctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcc           <  2:702756/92‑1 (MQ=255)                                                                            ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc          <  1:2196477/93‑1 (MQ=255)                                                                            ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc          <  2:1977690/93‑1 (MQ=255)                                                                            ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc          >  2:3019161/9‑101 (MQ=255)                                                                            ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc          >  2:1187555/9‑101 (MQ=255)                                                                             tcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCt         <  1:2090434/94‑1 (MQ=255)                                                                             tcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCt         <  2:3001313/94‑1 (MQ=255)                                                                              cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCTTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt        >  1:887951/7‑101 (MQ=255)                                                                              cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcccag        >  1:1977801/7‑99 (MQ=255)                                                                              cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt        >  2:5270947/7‑101 (MQ=255)                                                                              cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt        >  1:5486693/7‑101 (MQ=255)                                                                              cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt        >  1:16237/7‑101 (MQ=255)                                                                               gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc       <  1:4646275/96‑1 (MQ=255)                                                                               gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc       <  2:554090/96‑1 (MQ=255)                                                                               gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc       <  1:4094340/96‑1 (MQ=255)                                                                               gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc       <  2:4270070/96‑1 (MQ=255)                                                                               cggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc       >  2:5043085/6‑101 (MQ=255)                                                                                ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCg      >  1:1652462/5‑101 (MQ=255)                                                                                ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCg      >  1:322259/5‑101 (MQ=255)                                                                                ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCg      >  1:1784724/5‑101 (MQ=255)                                                                                  caaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGa    >  2:3024306/3‑101 (MQ=255)                                                                                  caaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGa    >  1:4160221/3‑101 (MQ=255)                                                                                   aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc   >  1:1746561/2‑101 (MQ=255)                                                                                   aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc   >  2:2621596/2‑101 (MQ=255)                                                                                   aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc   >  2:4844865/2‑101 (MQ=255)                                                                                   aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc   >  2:5229369/2‑101 (MQ=255)                                                                                   aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc   >  1:3509379/2‑101 (MQ=255)                                                                                    aaGCCCGAGGGGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAcc  <  1:2097996/101‑1 (MQ=255)                                                                                    aaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAcc  >  2:1068229/1‑101 (MQ=255)                                                                                    aaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAcc  >  2:628929/1‑101 (MQ=255)                                                                                    aaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAcc  <  1:1399098/101‑1 (MQ=255)                                                                                       |                                                                                                  AACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACC  >  NC_014034/2185919‑2186099 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG