Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 221,942 G→C 2.6% H239D (CAT→GAT)  RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034221,9420G→C 2.6% 5.2 ‑0.9 ‑0.1226H239D (CAT→GAT) RCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/2):  ref base (103/117):  total (107/119)
Fisher's exact test strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.19e-01

Only 100 of 262 total aligned reads displayed.

ACGGTCGAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCC  >  NC_014034/221842‑222042
                                                                                                    |                                                                                                     
acGGTCGAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATg                                                                                                       >  2:2335291/1‑101 (MQ=255)
acGGTCGAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATg                                                                                                       >  2:527837/1‑101 (MQ=255)
   gTCGAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.Gt                                                                                                   <  2:3943978/101‑1 (MQ=255)
     cGAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTgg                                                                                                 <  2:3832297/101‑1 (MQ=255)
         ccGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCtc                                                                                             <  2:773042/101‑1 (MQ=255)
          cGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCtcg                                                                                            <  1:341544/101‑1 (MQ=255)
              ttCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCtcggtcg                                                                                        <  2:2832245/101‑1 (MQ=255)
               tCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCtcggtcgg                                                                                       <  2:47649/101‑1 (MQ=255)
               tCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCtcggtcgg                                                                                       <  1:3907545/101‑1 (MQ=255)
               tCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCtcgggcgg                                                                                       >  1:4452585/1‑101 (MQ=255)
                cGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCgga                                                                                      <  1:4079656/101‑1 (MQ=255)
                 gCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggat                                                                                     >  2:3170159/1‑101 (MQ=255)
                 gCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggat                                                                                     >  1:4026728/1‑101 (MQ=255)
                  ccGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatc                                                                                    <  1:1989272/101‑1 (MQ=255)
                  ccGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatc                                                                                    <  2:1970394/101‑1 (MQ=255)
                  ccGGTGCCCGACACCGTCGCCGAGGCTTCGGTGATGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatc                                                                                    <  1:4974300/101‑1 (MQ=255)
                   cGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcg                                                                                   >  2:867918/1‑101 (MQ=255)
                     gTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggt                                                                                 >  2:1649174/1‑101 (MQ=255)
                      tGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtc                                                                                <  1:2231480/101‑1 (MQ=255)
                      tGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtc                                                                                <  1:1076952/101‑1 (MQ=255)
                       gCCCGATACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcg                                                                               <  1:952201/101‑1 (MQ=255)
                       gCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcg                                                                               <  2:1304390/101‑1 (MQ=255)
                           gACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcgggtc                                                                           <  1:138793/101‑1 (MQ=255)
                           gACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCCCGCTCTTCGACGGTGGGTGTCGGAGGC.GAGGGCTCGGTCggagcggtcggggc                                                                           >  1:2319973/1‑99 (MQ=255)
                            gcacCGTCGCCGGGGCTTTGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcgggtcc                                                                          <  2:2311967/100‑1 (MQ=255)
                             cacCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcgggtccg                                                                         <  2:4628118/101‑1 (MQ=255)
                             cacCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcggggccg                                                                         >  2:1551762/1‑101 (MQ=255)
                              acCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcgggtccgt                                                                        <  2:2782169/101‑1 (MQ=255)
                               ccGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcgggtccgtg                                                                       <  2:934239/101‑1 (MQ=255)
                               ccGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcgggtccggg                                                                       >  1:3039172/1‑99 (MQ=255)
                                 gTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcgggtccgtggg                                                                     <  2:2961259/101‑1 (MQ=255)
                                 gTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcgggtccgtggg                                                                     <  2:1386166/101‑1 (MQ=255)
                                    gCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcgggtccgtgggatc                                                                  <  1:1649268/101‑1 (MQ=255)
                                        aGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcgggtccgtgggatcggtc                                                              <  1:5730391/101‑1 (MQ=255)
                                        aGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcgggtccgtgggatcggtc                                                              <  2:5374910/101‑1 (MQ=255)
                                         ggCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcg                                                             <  1:2408704/101‑1 (MQ=255)
                                           cTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcggg                                                           <  1:4772206/101‑1 (MQ=255)
                                            ttCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcgggt                                                          <  2:2148308/101‑1 (MQ=255)
                                            ttCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcgggt                                                          <  1:4316893/101‑1 (MQ=255)
                                            ttCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcggggccgtgggatcggtcgggt                                                          >  2:4884190/1‑101 (MQ=255)
                                              cGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcgggtcc                                                        <  2:1797298/101‑1 (MQ=255)
                                               ggTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcgggcccg                                                       >  1:3324067/1‑101 (MQ=255)
                                                  gtTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGgatcggtcgggtccgtgggatcggtcgggtccgtgg                                                    >  1:4821949/1‑101 (MQ=255)
                                                  gtTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGgatcggtcgggtccgtgggatcggtcgggtccgtgg                                                    <  1:920932/101‑1 (MQ=255)
                                                    cgatggtCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGAtcgggcgggtccgtggggtcggccgggtgcgtggga                                                  >  2:4470511/4‑101 (MQ=255)
                                                          tCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCgggtccgtgggatcggtcgggtccgtgggatcggtc                                            >  2:2221995/1‑101 (MQ=255)
                                                          tCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCgggtccgtgggatcggtcgggtccgtgggatcggtc                                            <  1:2207169/101‑1 (MQ=255)
                                                           cAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGggtccgtgggatcggtcgggtccgtgggatcggtcg                                           >  1:4111094/1‑101 (MQ=255)
                                                            aGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGgtccgtgggatcggtcgggtccgtgggatcggtcgg                                          <  1:4209440/101‑1 (MQ=255)
                                                            aGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGgtccgtgggatcggtcgggtccgtgggatcggtcgg                                          <  2:2991851/101‑1 (MQ=255)
                                                              cGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGAt                                        <  2:4799924/101‑1 (MQ=255)
                                                              cGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGAt                                        <  2:1165835/101‑1 (MQ=255)
                                                               gTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCCGGTCCGTGGGGTCGGGCGGacc                                       >  2:5237227/1‑99 (MQ=255)
                                                                    cGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGGCCGTGGGGTCGGTCGGATCCGTgg                                  >  1:1154642/1‑101 (MQ=255)
                                                                     ggATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTggg                                 >  1:5685184/1‑101 (MQ=255)
                                                                      gATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGGCCGTGGGATCGGTCGGGTCCGTGGGa                                >  2:5112689/1‑101 (MQ=255)
                                                                       aTCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAt                               >  1:2497073/1‑101 (MQ=255)
                                                                       aTCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAt                               >  1:671945/1‑101 (MQ=255)
                                                                       aTCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAt                               <  1:3627000/101‑1 (MQ=255)
                                                                        tCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtc                              <  1:892390/101‑1 (MQ=255)
                                                                         cgcgCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcg                             >  1:2871713/1‑101 (MQ=255)
                                                                          gcgcTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcgg                            >  1:4060305/1‑101 (MQ=255)
                                                                          gcgcTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcgg                            <  1:5045283/101‑1 (MQ=255)
                                                                           cgcTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggt                           <  1:118875/101‑1 (MQ=255)
                                                                            gcTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtc                          >  2:3144217/1‑101 (MQ=255)
                                                                            gcTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtc                          <  1:1575881/101‑1 (MQ=255)
                                                                             cTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtcg                         <  2:629897/101‑1 (MQ=255)
                                                                             cTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtcg                         <  1:3248499/101‑1 (MQ=255)
                                                                             cTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTAGGATCCGAGGGAtcggtcg                         >  2:1513776/1‑101 (MQ=255)
                                                                                aTCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGt                      >  1:3722078/1‑101 (MQ=255)
                                                                                aTCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGt                      >  2:4353329/1‑101 (MQ=255)
                                                                                 tCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTc                     <  1:1515007/101‑1 (MQ=255)
                                                                                   gACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCg                   >  2:2542497/1‑101 (MQ=255)
                                                                                     ggggatcggTCGGGTCC.GTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGtc                 <  1:4903937/93‑2 (MQ=1)
                                                                                      gggttcgatCGGGTCC.GTGGGATCGGTCCGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGtcg                <  2:3578538/93‑3 (MQ=11)
                                                                                      gggatcggTCGGGTCC.GTGGGATCGGTCGGATCCGTGGGGCCCGTGGGATCGGTCGGGTCCGTGGGATCGGCCGGGTCCGTAGGAGCGGCTGGGTCCGtcg                >  2:2599995/8‑99 (MQ=2)
                                                                                      ggTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgg                <  2:4110845/101‑1 (MQ=255)
                                                                                      cgggtgCGTCGGATTCGGCGGGTTCGG.CGGGTCCGGCGGGTCCGGGGGATCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgg                <  2:2687867/96‑1 (MQ=2)
                                                                                        tGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTGGGGTCGGTgggg              >  2:2029514/1‑100 (MQ=18)
                                                                                        tGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggg              >  2:4227518/1‑100 (MQ=255)
                                                                                         gggCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAt             <  2:4066746/101‑1 (MQ=255)
                                                                                          tcggTCGGGTCC.GGGGGGTCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGtc            <  1:5099218/98‑1 (MQ=1)
                                                                                            gggggggggC.GTGGGATCGGTCGGATTCGTGGGGTCCGTTGGATCGGTCGGGTCCGTGGGATGGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGtccg          <  1:3106089/93‑3 (MQ=11)
                                                                                            ggTCGGGTCC.GTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGtccg          >  1:3538633/2‑99 (MQ=1)
                                                                                            cGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcgg          <  2:816508/101‑1 (MQ=255)
                                                                                             gTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGGCGGGTCCGTGGGAtcggg         >  2:3353016/1‑100 (MQ=255)
                                                                                              tggtaTGA.GTTGGTTCAGTGGGAGCGGTCGCATCCGTGTGATCGGTCGGGTCCGTGGGATCCGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtc        <  2:5723133/97‑1 (MQ=11)
                                                                                              tCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtc        <  1:4592732/101‑1 (MQ=255)
                                                                                               cGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcg       >  2:5494938/1‑101 (MQ=255)
                                                                                                ggATGG.GTGGGATCCGTGGGGTCGGTCGGGTGTGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcgg      <  1:5496234/101‑1 (MQ=11)
                                                                                                ggATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcgg      >  1:583036/1‑101 (MQ=255)
                                                                                                ggATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcgg      >  1:2713129/1‑101 (MQ=255)
                                                                                                ggATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcgg      >  2:1314129/1‑101 (MQ=255)
                                                                                                ggATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcgg      <  2:3007424/101‑1 (MQ=255)
                                                                                                ggATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcgg      >  2:34922/1‑101 (MQ=255)
                                                                                                  aTGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcggcc    >  1:5185634/1‑99 (MQ=255)
                                                                                                  aTGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGAt    >  1:5618691/1‑101 (MQ=255)
                                                                                                  aTGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGAt    >  1:2094894/1‑101 (MQ=255)
                                                                                                   tGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATc   <  1:5371721/101‑1 (MQ=255)
                                                                                                   tGC.GTGGGCTCGGTCGGATCGCTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATc   <  1:125234/101‑1 (MQ=255)
                                                                                                    |                                                                                                     
ACGGTCGAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGC.GTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCC  >  NC_014034/221842‑222042

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG