Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,317 G→A 2.3% intergenic (‑113/‑192) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3170G→A 2.3% 8.2 0.0 ‑0.0303intergenic (‑113/‑192)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (3/4):  ref base (133/163):  total (136/167)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.81e-01

Only 100 of 315 total aligned reads displayed.

ATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.CCCCC.TTCGCGGGCGGCCGCGACG  >  NC_014034/2249218‑2249415
                                                                                                     |                                                                                                       
  tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCGCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                                       <  1:219927/101‑1 (MQ=255)
  tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                                       >  1:1001972/1‑101 (MQ=255)
  tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                                       >  2:4227783/1‑101 (MQ=255)
    gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCaa                                                                                                     <  2:157140/101‑1 (MQ=255)
     tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAg                                                                                                    <  2:347935/101‑1 (MQ=255)
      gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                                   <  2:1347564/101‑1 (MQ=255)
        tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                                 <  2:4962380/101‑1 (MQ=255)
        tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                                 <  1:1856827/101‑1 (MQ=255)
        tCTTGAGATGCACAGATACCGCAGCTTGGGAACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                                 >  1:617163/1‑101 (MQ=255)
         cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                >  2:4832111/1‑101 (MQ=255)
         cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                <  1:876924/101‑1 (MQ=255)
         cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                <  2:2506593/101‑1 (MQ=255)
          ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                                               <  2:4695530/101‑1 (MQ=255)
          ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                                               <  2:5031176/101‑1 (MQ=255)
              gaTGCACAGATCCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                                        <  1:5258103/101‑1 (MQ=255)
              gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                                        <  1:1432384/101‑1 (MQ=255)
              gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                                        <  1:1922486/101‑1 (MQ=255)
              gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                                        <  2:1127087/101‑1 (MQ=255)
                tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggggg                                                                                      <  2:1659645/101‑1 (MQ=255)
                  cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCt                                                                                    <  1:1120856/101‑1 (MQ=255)
                  cacaGAAACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCt                                                                                    <  1:959722/101‑1 (MQ=255)
                    caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCttt                                                                                  >  1:5615551/1‑101 (MQ=255)
                    caGATACCGCAGCTTGGCCCCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCttt                                                                                  <  2:2464731/101‑1 (MQ=38)
                       aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCAt                                                                               <  2:4049277/101‑1 (MQ=255)
                         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCt                                                                             >  1:3582926/1‑101 (MQ=255)
                         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCt                                                                             <  2:4470016/101‑1 (MQ=255)
                         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCt                                                                             <  2:3311615/101‑1 (MQ=255)
                          ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCtt                                                                            <  1:382068/101‑1 (MQ=255)
                          ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCtt                                                                            <  1:1076568/101‑1 (MQ=255)
                          ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCtt                                                                            <  1:1316035/101‑1 (MQ=255)
                           cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTg                                                                           <  1:332820/101‑1 (MQ=255)
                           cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTg                                                                           <  2:2963652/101‑1 (MQ=255)
                           cGCAGCTGGGGCCCCGTGGGCAAGAAGGGGGATCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTg                                                                           <  2:804596/101‑1 (MQ=37)
                             cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGcc                                                                         >  2:2206460/1‑101 (MQ=255)
                             cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGcc                                                                         >  2:509718/1‑101 (MQ=255)
                              aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCg                                                                        <  1:4109309/101‑1 (MQ=255)
                               gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGACATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGa                                                                       >  1:5105964/1‑101 (MQ=255)
                               gCTTGGGCACCCGGGGCAAGAAGGGGCCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGa                                                                       <  2:3764183/101‑1 (MQ=255)
                                  tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaaaa                                                                    <  1:3061319/101‑1 (MQ=255)
                                  tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaaaa                                                                    >  2:3555088/1‑101 (MQ=255)
                                   gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                                   <  2:3637712/101‑1 (MQ=255)
                                   gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                                   >  2:4113417/1‑101 (MQ=255)
                                    ggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAata                                                                  <  1:5451581/101‑1 (MQ=255)
                                     gCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAatat                                                                 <  1:265060/101‑1 (MQ=255)
                                       aCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGGAAATATcc                                                               >  1:153183/1‑101 (MQ=255)
                                        cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATccc                                                              <  1:49086/101‑1 (MQ=255)
                                        cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATccc                                                              <  1:2900064/101‑1 (MQ=255)
                                        cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATccc                                                              <  2:5388484/101‑1 (MQ=255)
                                         ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCg                                                             <  1:46576/101‑1 (MQ=255)
                                          cGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgg                                                            <  1:2253609/101‑1 (MQ=255)
                                               cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                       <  1:2533352/101‑1 (MQ=255)
                                                aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGTTATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCAggggggag                                                      >  1:2369400/1‑99 (MQ=38)
                                                aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                      <  2:4910998/101‑1 (MQ=255)
                                                aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCAGAAAATATCCCGGGGGGTg                                                      <  1:4499628/101‑1 (MQ=255)
                                                aagaagGGGGCTCTGCCCCCGTCCCGGGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                      <  1:2059922/101‑1 (MQ=255)
                                                   aagGGGGCTCTGCCCCCTTCCCGTGCCGGGCCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                                   <  2:2017801/101‑1 (MQ=37)
                                                   aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                                   >  2:1497481/1‑101 (MQ=255)
                                                   aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                                   <  2:5175881/101‑1 (MQ=255)
                                                    agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg                                                  <  2:2840027/101‑1 (MQ=255)
                                                      ggggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                <  2:4654860/101‑1 (MQ=255)
                                                        ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcgc                                              <  2:1902201/101‑1 (MQ=255)
                                                        ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcgc                                              <  1:3463851/101‑1 (MQ=255)
                                                        ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTAATCTTGCCGAAAATATCCCGGGGGGGGAATGgcgc                                              >  1:1037231/1‑101 (MQ=38)
                                                         gctctccccccGTCCCGTGCCGGGACC.CCCCCGGGATTTTTGAGGAAAGAGGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCa                                             <  1:169553/94‑1 (MQ=37)
                                                          ctctGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAg                                            >  2:1104055/1‑101 (MQ=255)
                                                            ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc                                          >  1:3558700/5‑101 (MQ=255)
                                                            cggcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCGAGATGAA...GGTGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc                                          <  2:4812906/97‑1 (MQ=255)
                                                             tgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCa                                         <  2:3915410/98‑1 (MQ=255)
                                                                ccccGTCCCGGGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCagag                                      <  2:2080871/101‑1 (MQ=255)
                                                                     tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggggg                                 <  2:4370485/101‑1 (MQ=255)
                                                                     tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggggg                                 >  1:4342072/1‑101 (MQ=255)
                                                                       ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCCGAGGGGGGCa                               >  2:2572536/1‑101 (MQ=255)
                                                                       ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa                               >  2:2728021/1‑101 (MQ=255)
                                                                        cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg                              >  1:374616/1‑101 (MQ=255)
                                                                        cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAggggg                                  >  2:3850774/8‑101 (MQ=18)
                                                                         gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgc                             >  2:60989/1‑101 (MQ=255)
                                                                         gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAgggggg                                 <  1:668318/95‑1 (MQ=18)
                                                                          tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcg                            >  2:5691173/1‑101 (MQ=255)
                                                                          tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcg                            >  2:5475188/1‑101 (MQ=255)
                                                                          caagcggACTCCCCCCGGGATATTTGCAGCAAGCTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAGGGGGGc                                <  1:4983535/96‑1 (MQ=18)
                                                                            ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cc                         <  2:171451/101‑1 (MQ=255)
                                                                            ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cc                         >  2:2292392/1‑101 (MQ=255)
                                                                             cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccc                        >  1:1279654/1‑101 (MQ=255)
                                                                              gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cccc                       >  1:3995097/1‑101 (MQ=255)
                                                                               ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccccc                      >  1:4269649/1‑101 (MQ=255)
                                                                                  cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cccccctt                   <  1:3172465/101‑4 (MQ=255)
                                                                                  cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cccccctt                   <  1:4220541/101‑4 (MQ=255)
                                                                                   t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccccccttc                  >  2:4185741/1‑97 (MQ=255)
                                                                                      ccccGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCC.TTcgc                >  2:3213230/1‑101 (MQ=255)
                                                                                      ccccGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCC.TTcgc                >  2:2375026/1‑101 (MQ=255)
                                                                                      ccccGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCC.TTcgc                >  2:5608327/1‑101 (MQ=255)
                                                                                         cgcgATAATAGAGGCAAGCTGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.CCCCCATTCgcggg             <  2:2155548/98‑1 (MQ=25)
                                                                                          gggATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCGCGG.CCCCC.CTCgggggcg           >  1:2812371/1‑101 (MQ=37)
                                                                                          gggATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCC.TTCgcgggc            <  1:3268213/101‑1 (MQ=255)
                                                                                            gATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCC.TTCgcgggcgg          >  1:4712468/1‑101 (MQ=255)
                                                                                             atatTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCC.TTCGCGggcggc         >  2:5111094/1‑101 (MQ=255)
                                                                                                 ttGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCcgcg     <  2:1011146/101‑1 (MQ=255)
                                                                                                 ttGAGGCAAGAGGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCcgcg     <  2:4578113/101‑1 (MQ=255)
                                                                                                   gAGGCAAGATGAA...GGGGTGCGTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGAc   <  2:4309113/101‑1 (MQ=25)
                                                                                                    aGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGAcg  >  2:4950824/1‑101 (MQ=255)
                                                                                                     |                                                                                                       
ATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.CCCCC.TTCGCGGGCGGCCGCGACG  >  NC_014034/2249218‑2249415

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG