Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,331 G→T 2.7% intergenic (‑127/‑178) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3310G→T 2.7% 9.7 0.0 ‑0.2255intergenic (‑127/‑178)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (3/4):  ref base (113/135):  total (116/139)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.64e-01

Only 100 of 290 total aligned reads displayed.

GATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGT  >  NC_014034/2249232‑2249430
                                                                                                         |                                                                                                          
gaTGCACAGATCCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                                                            <  1:5258103/101‑1 (MQ=255)
gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                                                            <  2:2146186/101‑1 (MQ=255)
gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                                                            >  1:3809745/1‑101 (MQ=255)
 aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..g                                                                                                         <  2:4422066/101‑1 (MQ=255)
  tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..gg                                                                                                        >  1:3335318/1‑101 (MQ=255)
  tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..gg                                                                                                        <  2:1659645/101‑1 (MQ=255)
   gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGc                                                                                                       >  2:5463407/1‑101 (MQ=255)
    cacaGATACCGCATCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCt                                                                                                      <  1:2854235/101‑1 (MQ=255)
    cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCt                                                                                                      <  2:10969/101‑1 (MQ=255)
     acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCtt                                                                                                     >  2:4342402/1‑101 (MQ=255)
      caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCttt                                                                                                    <  1:2057049/101‑1 (MQ=255)
      caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCttt                                                                                                    >  2:4825574/1‑101 (MQ=255)
      caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCttt                                                                                                    <  1:4700531/101‑1 (MQ=255)
       aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                                   >  1:3071001/1‑101 (MQ=255)
         aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCAt                                                                                                 <  2:1781233/101‑1 (MQ=255)
           aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                               <  1:5029491/101‑1 (MQ=255)
           aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                               <  2:4470016/101‑1 (MQ=255)
           aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                               >  1:3582926/1‑101 (MQ=255)
           aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                               >  2:2059805/1‑101 (MQ=255)
             cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTg                                                                                             <  2:2963652/101‑1 (MQ=255)
             cGCAGCTGGGGCCCCGTGGGCAAGAAGGGGGATCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTg                                                                                             <  2:804596/101‑1 (MQ=37)
              gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGc                                                                                            <  1:5171188/101‑1 (MQ=255)
               cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGcc                                                                                           >  2:509718/1‑101 (MQ=255)
                aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                                          <  2:4669100/101‑1 (MQ=255)
                aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                                          <  1:4109309/101‑1 (MQ=255)
                 gCTTGGGCACCCGGGGCAAGAAGGGGCCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGa                                                                                         <  2:3764183/101‑1 (MQ=255)
                  cTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                        >  1:746155/1‑101 (MQ=255)
                    tGGGCACGCGGGGCAAGAAATGGGCTCT.GCCCCCGTCCCGTGGCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGAG..GGCTTTCATCTTGCCGaaaa                                                                                      <  1:3279869/101‑1 (MQ=25)
                    tGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                                      >  2:3555088/1‑101 (MQ=255)
                    tGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                                      >  1:2212618/1‑101 (MQ=255)
                     gggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                     <  2:3637712/101‑1 (MQ=255)
                      ggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAata                                                                                    <  2:4960847/101‑1 (MQ=255)
                      ggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAata                                                                                    <  1:5451581/101‑1 (MQ=255)
                         aCCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGGAAATATcc                                                                                 >  1:153183/1‑101 (MQ=255)
                         aCCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTCCCGAAAATATcc                                                                                 <  1:3126609/101‑1 (MQ=255)
                            cGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                                              >  2:3257542/1‑101 (MQ=255)
                              gggCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggg                                                                            <  2:5329162/101‑1 (MQ=255)
                                 gaagcagGGGGCTCTCC.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                         <  1:912409/100‑1 (MQ=37)
                                 gaagaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                         <  1:1483221/100‑1 (MQ=255)
                                 cAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                         <  1:5671797/101‑1 (MQ=255)
                                  aagaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggggg                                                                        >  1:2575173/1‑99 (MQ=255)
                                  aagaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggggg                                                                        >  1:1957463/1‑99 (MQ=255)
                                  aagaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                        <  2:3369866/101‑1 (MQ=255)
                                  aagaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCAGAAAATATCCCGGGGGGTg                                                                        <  1:4499628/101‑1 (MQ=255)
                                   agaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                       <  1:2353482/101‑1 (MQ=255)
                                    gaagGGGGCTCG.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                      <  1:2945530/101‑1 (MQ=255)
                                     aagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                    <  2:249762/101‑1 (MQ=255)
                                     aagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                    <  2:5175881/101‑1 (MQ=255)
                                     aagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                    >  2:1497481/1‑101 (MQ=255)
                                     aagGGGGCTCT.GCCCCCGTCCCGTGCCCGCACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                    <  1:2256815/101‑1 (MQ=37)
                                      agGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                   >  2:2174038/1‑101 (MQ=255)
                                        ggggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                 <  2:4943000/101‑1 (MQ=255)
                                        ggggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                 <  2:5601140/101‑1 (MQ=255)
                                          ggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                               <  1:3463851/101‑1 (MQ=255)
                                           gCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                              >  1:4309378/1‑101 (MQ=255)
                                               ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                         >  1:3558700/5‑101 (MQ=255)
                                                tgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCa                                                        <  2:3915410/98‑1 (MQ=255)
                                                tccccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..CCCa                                                        <  2:4908152/98‑1 (MQ=255)
                                                 gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCGAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcccg                                                       >  2:3091455/3‑99 (MQ=255)
                                                 gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                       >  2:3855116/3‑101 (MQ=255)
                                                    cacatccagtACCGGGACT.CCCCCGGGATATTTGAGGCAAGCTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgg                                                    <  2:5704248/97‑1 (MQ=37)
                                                       gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                 <  1:251357/101‑1 (MQ=255)
                                                        tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                <  2:4370485/101‑1 (MQ=255)
                                                          ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCCGAGGGGGGCa                                              >  2:2572536/1‑101 (MQ=255)
                                                          ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                              <  1:4941439/101‑1 (MQ=255)
                                                          ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                              >  2:2728021/1‑101 (MQ=255)
                                                           cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                             >  1:374616/1‑101 (MQ=255)
                                                            gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c                                           <  1:2671209/101‑1 (MQ=255)
                                                            gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                >  2:4767926/7‑101 (MQ=18)
                                                               ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc                                       >  2:2292392/1‑101 (MQ=255)
                                                               ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc                                       <  2:171451/101‑1 (MQ=255)
                                                                 gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccc                                     >  1:1861457/1‑101 (MQ=255)
                                                                  ggccT.CCCCCGGGATATTGGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccc                                    <  1:1024759/98‑1 (MQ=255)
                                                                  ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccc                                    <  2:5644335/101‑1 (MQ=255)
                                                                  ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccc                                    >  1:4269649/1‑101 (MQ=255)
                                                                  ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccc                                    >  2:4023660/1‑101 (MQ=255)
                                                                     cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GTCTTTCATCTGGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccccctt                                 <  2:5105679/101‑4 (MQ=255)
                                                                     cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccccctt                                 <  1:4220541/101‑4 (MQ=255)
                                                                      t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttc                                >  2:4185741/1‑97 (MQ=255)
                                                                         ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTcgc                               >  2:2375026/1‑101 (MQ=255)
                                                                          cccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CCCCCtgc                                 >  1:1041015/1‑99 (MQ=2)
                                                                          cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTcgcg                              >  2:1122441/1‑101 (MQ=255)
                                                                            cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CCCCCtgccc                               >  2:4065635/1‑97 (MQ=2)
                                                                              ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCgcgggcg                          >  1:1103886/1‑101 (MQ=255)
                                                                                 ttttggAGGAAAGAAAAG...GGGG..GGTTTTAATCTTGCCAAAAATATCCGGGGGGGTGAA.TGGCGCA..GCAAGGGGGGGGCAGCCC.CCCCCTTCGCGGGCGGcc                       <  2:1175788/96‑1 (MQ=255)
                                                                                 tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGcc                       <  1:5614770/101‑1 (MQ=255)
                                                                                 tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAAGATCCGGGGGGGTGAA.GGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGcc                       <  1:1374223/101‑1 (MQ=37)
                                                                                    ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCcgcg                    <  2:1011146/101‑1 (MQ=255)
                                                                                    ttGAGGCAAGAGGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCcgcg                    <  2:4578113/101‑1 (MQ=255)
                                                                                     tGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGa                   >  1:1217705/1‑101 (MQ=255)
                                                                                         gCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgcg               <  2:2212496/101‑1 (MQ=255)
                                                                                          cAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgcgc              >  1:4014290/1‑101 (MQ=255)
                                                                                             gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGcgcc           >  1:5389961/1‑101 (MQ=255)
                                                                                                gAA...GGGG..GGCTTTCATCTGGCCGAAAATGTCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa        <  1:475399/101‑1 (MQ=37)
                                                                                                 aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa       >  1:3178166/1‑101 (MQ=255)
                                                                                                 aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa       >  1:1283618/1‑101 (MQ=255)
                                                                                                  a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc      <  1:1692353/101‑1 (MQ=255)
                                                                                                  a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc      <  2:1951910/101‑1 (MQ=255)
                                                                                                   gatggtg..ggCTTTCAACTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa        <  2:744295/95‑1 (MQ=25)
                                                                                                        gg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg   <  2:284442/101‑1 (MQ=255)
                                                                                                         |                                                                                                          
GATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGT  >  NC_014034/2249232‑2249430

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG