Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,359 G→A 2.1% intergenic (‑155/‑150) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3590G→A 2.1% 4.9 ‑1.0 ‑0.8240intergenic (‑155/‑150)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (116/118):  total (120/120)
Fisher's exact test strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.54e-01

Only 100 of 281 total aligned reads displayed.

CGGGGCAAGAAGGGGGCTCT.G.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCC  >  NC_014034/2249260‑2249458
                                                                                                           |                                                                                                        
cGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                                                                          <  1:2253609/101‑1 (MQ=255)
 ggggCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggg                                                                                                         <  2:1109196/101‑1 (MQ=255)
  gggCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggg                                                                                                        <  2:5329162/101‑1 (MQ=255)
   ggCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggg                                                                                                       >  2:2530781/1‑101 (MQ=255)
    gCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggg                                                                                                      >  1:1531085/1‑101 (MQ=255)
     gaagcagGGGGCTCTCC.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                                                     <  1:912409/100‑1 (MQ=37)
     gaagaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                                                     <  1:1483221/100‑1 (MQ=255)
     cAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                                                     <  2:2671403/101‑1 (MQ=255)
     cAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                                                     <  1:3315069/101‑1 (MQ=255)
     cAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                                                     <  1:2533352/101‑1 (MQ=255)
      aagaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGTTATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCAggggggag                                                                                                    >  1:2369400/1‑99 (MQ=38)
      aagaagGGGGCTCT.GCCCCCGTCCAGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                                    >  2:2474096/1‑101 (MQ=255)
       agaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                                                   <  2:3869322/101‑1 (MQ=255)
       agaagGGGGCTCG.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTCCTGAAAATATCCCGTGGGGTGa                                                                                                   <  2:191458/101‑1 (MQ=37)
        gaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                                                  <  2:1758733/101‑1 (MQ=255)
        gaagGGGGCTCG.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                                                  <  1:2945530/101‑1 (MQ=255)
         aagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                                                <  2:4498615/101‑1 (MQ=255)
         aagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                                                >  2:1497481/1‑101 (MQ=255)
          agGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                                               >  2:2174038/1‑101 (MQ=255)
          agGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                                               <  2:2840027/101‑1 (MQ=255)
            ggggCTCTCC.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                                             <  1:1282682/101‑1 (MQ=255)
            ggggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                                             <  2:5601140/101‑1 (MQ=255)
            ggggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                                             <  2:4943000/101‑1 (MQ=255)
              ggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                                                           <  1:3463851/101‑1 (MQ=255)
                ctct.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                                                       <  2:3985903/101‑1 (MQ=255)
                     gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                                   >  2:3855116/3‑101 (MQ=255)
                     gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                                   >  1:3687868/3‑101 (MQ=255)
                      cccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCaga                                                                                  <  2:2317432/100‑1 (MQ=255)
                            tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                                            >  1:4342072/1‑101 (MQ=255)
                              ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.Ca                                                                         <  1:4941439/101‑1 (MQ=255)
                              ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGG.cg                                                                         >  1:1104936/1‑100 (MQ=255)
                               cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAg                                                                        <  2:5103290/101‑1 (MQ=255)
                               cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAg                                                                        <  2:4629769/101‑1 (MQ=255)
                               cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAg                                                                        >  1:3071048/1‑101 (MQ=255)
                                gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAgc                                                                       >  2:4357325/1‑101 (MQ=255)
                                gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAgc                                                                       >  1:3575561/1‑101 (MQ=255)
                                gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                            >  2:4767926/7‑101 (MQ=18)
                                gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                            <  1:668318/95‑1 (MQ=18)
                                  gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAgcg.c                                                                    >  1:2606070/1‑101 (MQ=255)
                                     gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCG.cccc                                                                 >  1:3995097/1‑101 (MQ=255)
                                      ggccT.CCCCCGGGATATTGGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCG.ccccc                                                                <  1:1024759/98‑1 (MQ=255)
                                      ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGCGAA..GCCAGAGGGGGG.CTgcg.c                                                                    >  2:4956631/1‑101 (MQ=17)
                                      ggACT.CCCCGGGGATATTTGAGGCAAGATGAA...GGGG..GCCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCG.ccccc                                                                <  2:5615259/101‑1 (MQ=255)
                                       gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCG.cccccc                                                               >  1:1672024/1‑100 (MQ=255)
                                          t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCG.ccccccttc                                                            >  2:4185741/1‑97 (MQ=255)
                                          t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCG.ccccccttc                                                            >  2:580828/1‑97 (MQ=255)
                                              cccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGG.CAGCG.CCCCCtgc                                                             >  1:1041015/1‑99 (MQ=2)
                                                cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGG.CAGCG.CCCCCtgccc                                                           >  2:4065635/1‑97 (MQ=2)
                                                cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCgcggg                                                        <  1:3766694/101‑1 (MQ=255)
                                                cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCgcggg                                                        <  1:5463697/101‑1 (MQ=255)
                                                 gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCgcgggc                                                       <  2:4246316/101‑1 (MQ=255)
                                                  ggATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGG.CAGCG.CCCCCtgcaccg                                                         <  2:4900346/101‑7 (MQ=0)
                                                   gATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCgcgggcgg                                                     >  1:4712468/1‑101 (MQ=255)
                                                    atatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGggcggc                                                    >  2:5111094/1‑101 (MQ=255)
                                                     tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGcc                                                   <  1:5614770/101‑1 (MQ=255)
                                                       tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCcgc                                                 >  2:2914001/1‑101 (MQ=255)
                                                        ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGccaga                                                >  2:5220671/1‑98 (MQ=255)
                                                             gCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGAcgcg                                           <  2:2212496/101‑1 (MQ=255)
                                                                 gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGcgcc                                       >  1:5389961/1‑101 (MQ=255)
                                                                 gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGcgcc                                       <  1:428638/101‑1 (MQ=255)
                                                                 gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGcgcc                                       <  2:3335113/101‑1 (MQ=255)
                                                                 gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGcgcc                                       >  1:4795376/1‑101 (MQ=255)
                                                                    gga...GGGG..GGTTTTTCTTTTTGCGAAAATTGCCCGGGGGGGGAAGGGGCCCC..GCCGGGGGGGGGCCCCCC.CCCCCTTCGCGGGCGGCCGCGACGCGcgccgc                                     <  1:1965046/99‑1 (MQ=255)
                                                                    gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGA.GGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa                                   >  2:5670334/1‑101 (MQ=255)
                                                                     aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa                                   >  1:3178166/1‑101 (MQ=255)
                                                                       gatggtg..ggCTTTCAACTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                                    <  2:744295/95‑1 (MQ=25)
                                                                          gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                                 >  2:3586704/1‑101 (MQ=255)
                                                                           ggg..ggTTTTCATCTTGCCGAAAAAACCCGGGGGGGTGAA.TGGCGCA..GCCAGGGGGGGC.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                <  1:4228043/101‑1 (MQ=37)
                                                                             g..ggCTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGt                              <  1:2292518/101‑1 (MQ=255)
                                                                             g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGGGCA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCTCGGGCGGCAGGGACGCGGGACGCAACAgga                              >  2:4375029/1‑100 (MQ=21)
                                                                                  cTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGt                           <  1:4143724/101‑1 (MQ=255)
                                                                                  cTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGt                           <  1:1916433/101‑1 (MQ=255)
                                                                                   tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CGGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCggc                          >  2:5482441/1‑99 (MQ=255)
                                                                                   tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                          >  1:5154420/1‑101 (MQ=255)
                                                                                   tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.GGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                          <  1:1366222/101‑1 (MQ=255)
                                                                                    ttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc                         >  2:4698769/1‑101 (MQ=255)
                                                                                      cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGCCGTGccc                       >  1:566588/1‑101 (MQ=255)
                                                                                       aTCTTGCCGAAAATATCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                      <  1:1243856/101‑1 (MQ=255)
                                                                                        tCTTGCCGAAAGTATCCCGGGGGGTGGA.TGGCGCA..GCCAGGGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCACAACAGGTCGTGCCCCt                     >  2:3758708/1‑101 (MQ=37)
                                                                                        tCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTTCCCCt                     >  1:452330/1‑101 (MQ=255)
                                                                                          ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                   >  2:3708063/1‑101 (MQ=255)
                                                                                           tGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTg                  <  2:4277762/101‑1 (MQ=255)
                                                                                           tGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTg                  <  1:5702318/101‑1 (MQ=255)
                                                                                            gCCGAAAATATCCCAGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt                 >  2:1020012/1‑101 (MQ=255)
                                                                                             ccGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                >  2:2255458/1‑101 (MQ=255)
                                                                                             ccGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                <  2:1861347/101‑1 (MQ=255)
                                                                                              cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa               <  2:787291/101‑1 (MQ=255)
                                                                                               gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt              >  1:269632/1‑101 (MQ=255)
                                                                                               gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt              <  2:1671925/101‑1 (MQ=255)
                                                                                                 aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc            >  1:3514480/1‑101 (MQ=255)
                                                                                                   atatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGACGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTg          >  2:341770/1‑101 (MQ=255)
                                                                                                    tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGt         <  1:1828752/101‑1 (MQ=255)
                                                                                                     agcagggggggTGAG.GGGCGCT..GCGAGAGGGGGG.CAACGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc        <  1:206421/96‑1 (MQ=255)
                                                                                                        ccGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCa     <  2:2575031/101‑1 (MQ=255)
                                                                                                         cGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc    >  2:2127671/1‑101 (MQ=255)
                                                                                                         cGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc    <  2:3195757/101‑1 (MQ=255)
                                                                                                          ggggggTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc   >  2:1095929/1‑101 (MQ=255)
                                                                                                          ggggggTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGCGCCCCTTTGTCATTCTGTCGCAcc   >  1:2786991/1‑101 (MQ=255)
                                                                                                           gggggTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc  >  2:2052066/1‑101 (MQ=255)
                                                                                                           gggggTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc  >  2:12716/1‑101 (MQ=255)
                                                                                                           |                                                                                                        
CGGGGCAAGAAGGGGGCTCT.G.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGG.CAGCG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCC  >  NC_014034/2249260‑2249458

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG