BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,262,586	T→C	100%	Q55R (CAG→CGG)	RCAP_rcc02105 ←	IS3/IS911 family transposase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,262,586	0	T→C	100.0%	915.4	252	Q55R (CAG→CGG)	RCAP_rcc02105	IS3/IS911 family transposase
Reads supporting (aligned to +/- strand): new base (165/87): ref base (0/0): total (165/87)

Only 100 of 297 total aligned reads displayed.


ATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCGTCAGCTGCTTGGCATCGGACAAATCCATCCCACCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCGG  >  NC_014034/2262488‑2262683


aTTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgccgc                                                                                                 >  1:3365765/1‑101 (MQ=255)


aTTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgccgc                                                                                                 >  2:222587/1‑101 (MQ=255)


aTTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgccgc                                                                                                 <  2:250283/101‑1 (MQ=255)


 ttGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgccgct                                                                                                <  2:1893729/101‑1 (MQ=255)


  tGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCtt                                                                                               <  1:4795211/101‑1 (MQ=255)


  tGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCtt                                                                                               <  2:1102100/101‑1 (MQ=255)


   gggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCACACTGCTTCAGCCGCTTg                                                                                              >  1:2726565/1‑101 (MQ=255)


   gggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTg                                                                                              >  2:5375503/1‑101 (MQ=255)


    ggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTgg                                                                                             <  1:525394/101‑1 (MQ=255)


    ggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTgg                                                                                             >  2:3373342/1‑101 (MQ=255)


    ggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTgg                                                                                             >  1:495185/1‑101 (MQ=255)


     gCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGc                                                                                            >  2:5431031/1‑101 (MQ=255)


        tGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATc                                                                                         >  1:3060769/1‑101 (MQ=255)


        tGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATc                                                                                         >  2:4806303/1‑101 (MQ=255)


          aaCCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCgg                                                                                       <  2:685947/101‑1 (MQ=255)


            ccAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGAc                                                                                     >  1:435825/1‑101 (MQ=255)


             cAATGGCGCCACACTGGCTCGATCCAGAATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACa                                                                                    <  2:1996483/101‑1 (MQ=255)


                  gcgcCACCCTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcc                                                                               <  2:5672655/101‑1 (MQ=255)


                  gcgcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcc                                                                               <  1:1306930/101‑1 (MQ=255)


                    gcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccat                                                                             >  1:1837614/1‑101 (MQ=255)


                    gcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccat                                                                             >  2:5411381/1‑101 (MQ=255)


                      cacaCTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccatcc                                                                           >  1:3396971/1‑101 (MQ=255)


                      cacaCTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccatcc                                                                           <  2:3347784/101‑1 (MQ=255)


                       ccacTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATccc                                                                          <  1:4503795/100‑1 (MQ=255)


                       acacTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATccc                                                                          >  2:1552618/1‑101 (MQ=255)


                        cacTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATcccg                                                                         >  2:2682104/1‑100 (MQ=255)


                          cTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCccgcc                                                                       <  1:5375793/101‑1 (MQ=255)


                              cTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATAc                                                                   >  1:2574928/1‑101 (MQ=255)


                               tCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACt                                                                  >  2:4890434/1‑101 (MQ=255)


                                cGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACtt                                                                 >  1:4191622/1‑101 (MQ=255)


                                cGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACtt                                                                 >  2:3689549/1‑101 (MQ=255)


                                cGATCCAGCATCACACCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAAGTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACtt                                                                 <  2:3937298/101‑1 (MQ=18)


                                  aTCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTgg                                                               >  1:657502/1‑101 (MQ=255)


                                     cAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCt                                                            >  1:4695825/1‑101 (MQ=255)


                                      aGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCtt                                                           >  1:5047305/1‑101 (MQ=255)


                                      aGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCACACTTGGCCtt                                                           >  1:2431544/1‑101 (MQ=18)


                                       gCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTc                                                          >  1:349092/1‑101 (MQ=255)


                                        cATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCa                                                         <  1:2051521/101‑1 (MQ=255)


                                         aTCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAg                                                        >  1:1423142/1‑101 (MQ=255)


                                          tCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGc                                                       >  2:4224297/1‑101 (MQ=255)


                                          acacaCCCGCAACCAGCGGCTCCAGCTTGCTTTTCTCTGCCGCAAACTGCTACGCCCTCTTGCCATCGGACAAACCCACCCCGCCATACTTGGCCTTCAGc                                                       <  1:4111466/100‑1 (MQ=255)


                                              ccccGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGt                                                   <  1:103203/99‑1 (MQ=18)


                                              acccGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGAAAAATCCATCCCGCCATACTTGGCCTTCAGCTTGt                                                   <  1:1666008/99‑1 (MQ=17)


                                               tCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTa                                                  >  2:3123882/1‑101 (MQ=255)


                                                ccGCCACCAGGCGCTTCAGCTTGGCTTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAg                                                 <  2:2979183/101‑1 (MQ=18)


                                                 cGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGa                                                <  2:880657/101‑1 (MQ=255)


                                                 cGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGa                                                >  1:1776889/1‑101 (MQ=255)


                                                  gCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGaa                                               >  1:1683937/1‑101 (MQ=255)


                                                   cccccaGGCGCTTCAGCTTGGCGTTCTCTCCCTCAAACTGCTTCAGCCGCTTGGCATGGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAg                                              <  1:3610398/98‑1 (MQ=18)


                                                   ccaccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAg                                              <  1:3221592/101‑1 (MQ=255)


                                                   ccaccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAg                                              >  1:2167769/1‑101 (MQ=255)


                                                    cacgaGGCGCTTCATCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGCCAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAgg                                             >  2:3630319/1‑101 (MQ=18)


                                                     cccaGGCGCTTCAGCTTGGCTTTCTCTCCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGt                                            <  2:1957274/100‑1 (MQ=18)


                                                     cccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGt                                            <  2:495135/100‑1 (MQ=255)


                                                     cccaGGCGCTTCAGCTTGGCGTTCTCTCCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGt                                            <  2:382443/100‑1 (MQ=18)


                                                     accaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGt                                            >  2:4605507/1‑101 (MQ=255)


                                                      ccgcgcgcTTCAGTTTGGCGTTCCCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCAGCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTc                                           <  2:3864474/97‑1 (MQ=18)


                                                      ccaGGCTCTTCATCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTc                                           <  1:2529944/101‑1 (MQ=18)


                                                      ccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTc                                           <  1:763540/101‑1 (MQ=255)


                                                       caGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcg                                          <  1:2200783/101‑1 (MQ=255)


                                                       caGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcg                                          >  1:1472503/1‑101 (MQ=255)


                                                        aGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcgc                                         >  2:3320203/1‑101 (MQ=255)


                                                        aGGCCCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcgc                                         <  2:440536/101‑1 (MQ=18)


                                                         ggCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcgcg                                        >  2:4855665/1‑101 (MQ=255)


                                                          gcgcTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCgg                                       <  2:5612736/101‑1 (MQ=255)


                                                          gcgcTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCgg                                       <  1:1366822/101‑1 (MQ=255)


                                                           cgcTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCggg                                      <  2:5062125/101‑1 (MQ=21)


                                                           cgcTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGAACAAACCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCggg                                      <  2:2829797/101‑1 (MQ=21)


                                                              ttCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATGCGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCt                                   >  2:5002040/1‑101 (MQ=18)


                                                              ttCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCt                                   >  1:865358/1‑101 (MQ=255)


                                                               tCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACACATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTg                                  >  1:2273658/1‑101 (MQ=21)


                                                                cAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGa                                 >  2:2385242/1‑101 (MQ=255)


                                                                cAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGa                                 >  2:1158130/1‑101 (MQ=255)


                                                                  gCTGGGCGTTCTCTCCCTCAAACGGCTTCAGCCCCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGc                               <  2:2431410/101‑1 (MQ=21)


                                                                   cTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGcc                              >  2:1300463/1‑101 (MQ=255)


                                                                     tGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCa                            >  1:5767500/1‑101 (MQ=25)


                                                                      ggCTTTCTCTTCCTCAACCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCAt                           <  2:3742126/101‑1 (MQ=25)


                                                                      ggCGTTCTCTCCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCAt                           <  1:1203423/101‑1 (MQ=25)


                                                                       gCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCTATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATg                          <  2:3155637/101‑1 (MQ=25)


                                                                        cGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGc                         >  2:2198924/1‑101 (MQ=25)


                                                                         gTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCt                        >  2:1015027/1‑101 (MQ=255)


                                                                          ttCTCTTCCCCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCtt                       <  1:3885336/101‑1 (MQ=25)


                                                                           tctcTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTc                      >  1:1258103/1‑101 (MQ=255)


                                                                            ctctTCCTCAACCTGCTTCAGCCGCTTGGCATCGAACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTcc                     <  1:1946541/101‑1 (MQ=25)


                                                                             tctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCg                    >  2:956820/1‑101 (MQ=37)


                                                                               ttCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGc                  >  1:34446/1‑101 (MQ=255)


                                                                                   tCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAg              <  1:2927817/101‑1 (MQ=255)


                                                                                    cAAACTGCTCCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGc             <  1:3707317/101‑1 (MQ=25)


                                                                                      aaCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTc           <  2:1379047/101‑1 (MQ=255)


                                                                                      aaCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTc           <  2:172463/101‑1 (MQ=255)


                                                                                        cTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCgg         >  1:4083923/1‑101 (MQ=255)


                                                                                         tGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTTTATAAGATCGCGGGGCTGAGCCCATGCTTCCGGCAAGGCTCGGa        >  2:5756111/1‑101 (MQ=21)


                                                                                         tGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGa        >  2:1224873/1‑101 (MQ=255)


                                                                                         tGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGa        >  2:2157046/1‑101 (MQ=255)


                                                                                          gcttCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGaa       >  1:5473927/4‑101 (MQ=255)


                                                                                            ttCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGt     >  2:5787857/2‑101 (MQ=255)


                                                                                             tCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTc    >  1:3110939/1‑101 (MQ=255)


                                                                                              cAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCg   >  1:1067837/1‑101 (MQ=255)


                                                                                               aGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCgg  >  1:3772141/1‑101 (MQ=255)


                                                                                               aGCCGCTGGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCgg  <  1:4305718/101‑1 (MQ=255)


ATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCGTCAGCTGCTTGGCATCGGACAAATCCATCCCACCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCGG  >  NC_014034/2262488‑2262683

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 26 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG