Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,389,542 C→G 100% A413A (GCC→GCG ackA2 → acetate kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,389,5420C→G100.0% 634.8 168A413A (GCC→GCGackA2acetate kinase
Reads supporting (aligned to +/- strand):  new base (99/69):  ref base (0/0):  total (99/69)

Only 100 of 195 total aligned reads displayed.

ACGGACGAGGAACGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCCCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAGCGCGGAACTTTCGGGCCATCGGGCCG  >  NC_014034/2389444‑2389639
                                                                                                    |                                                                                                  
acggacgAGGAACGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCa                                                                                                  <  2:269313/101‑1 (MQ=255)
acggacgAGGAACGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCa                                                                                                  <  2:1414381/101‑1 (MQ=255)
acggacgAGGAACGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCa                                                                                                  >  1:4154100/1‑101 (MQ=255)
 cggacgAGGAACGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCaa                                                                                                 <  1:3762888/101‑1 (MQ=255)
  ggacgAGGAACGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAAt                                                                                                <  2:3874097/101‑1 (MQ=255)
  ggacgAGGAACGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAAt                                                                                                <  2:708838/101‑1 (MQ=255)
   gacgAGGAACGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATg                                                                                               >  1:5226427/1‑101 (MQ=255)
   gacgAGGAACGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATg                                                                                               <  1:424380/101‑1 (MQ=255)
   gacgAGGAACGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATg                                                                                               <  1:1966282/101‑1 (MQ=255)
     cgAGGAACGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATgag                                                                                             <  1:1153812/101‑1 (MQ=255)
       aGGAACGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.ACGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGAc                                                                                           >  1:5008686/1‑101 (MQ=255)
       aGGAACGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGAc                                                                                           >  1:2331964/1‑101 (MQ=255)
       aGGAACGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGAc                                                                                           <  1:1090639/101‑1 (MQ=255)
       aGGAACGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATAAGAc                                                                                           >  2:4287230/1‑101 (MQ=255)
        ggAACGGATGATCGCGATCGACCCCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGAcc                                                                                          <  1:1371773/101‑1 (MQ=255)
        ggAACGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGAcc                                                                                          >  1:5764525/1‑101 (MQ=255)
         gAACGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCg                                                                                         >  2:5069044/1‑101 (MQ=255)
          aaCGGATTATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCgg                                                                                        <  1:1203921/101‑1 (MQ=255)
          aaCGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCgg                                                                                        <  2:354317/101‑1 (MQ=255)
          aaCGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCgg                                                                                        >  1:4749852/1‑101 (MQ=255)
            cGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGc                                                                                      <  2:4329933/101‑1 (MQ=255)
              gatgatCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCaa                                                                                    <  1:3725537/101‑1 (MQ=255)
               atgatCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAg                                                                                   <  1:3820177/101‑1 (MQ=255)
               atgatCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAg                                                                                   >  2:2010631/1‑101 (MQ=255)
                 gatCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAggg                                                                                 >  2:3826456/1‑101 (MQ=255)
                  atCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAgggg                                                                                >  1:3237010/1‑101 (MQ=255)
                    cgcgATCGACACCGCGGCTTTGACGGCCGCCGCGTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAgggggg                                                                              <  1:5010157/101‑1 (MQ=255)
                     gcgATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.c                                                                            >  2:148421/1‑101 (MQ=255)
                      cgATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.cc                                                                           <  1:1041422/101‑1 (MQ=255)
                       gATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.Ccg                                                                          >  2:3301860/1‑101 (MQ=255)
                          cGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCgcgc                                                                       <  2:3908453/101‑1 (MQ=255)
                          cGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCgcgc                                                                       <  1:1453644/101‑1 (MQ=255)
                           gACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCa                                                                      <  2:573195/101‑1 (MQ=255)
                           gACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCa                                                                      <  1:2090723/101‑1 (MQ=255)
                           gACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCa                                                                      <  1:1897912/101‑1 (MQ=255)
                           gACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCa                                                                      >  1:3504812/1‑101 (MQ=255)
                            ccacCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAg                                                                     <  2:1504176/100‑1 (MQ=255)
                            acacCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAg                                                                     >  1:3355244/1‑101 (MQ=255)
                             cacCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAgc                                                                    <  2:5117948/101‑1 (MQ=255)
                              acCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCC.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGGAgcg                                                                   >  1:840092/1‑101 (MQ=255)
                               ccGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAgcgc                                                                  <  2:1910856/101‑1 (MQ=255)
                                 gcgGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAgcgcgc                                                                >  2:4558513/1‑101 (MQ=255)
                                  cgGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGcc                                                               >  2:1005818/1‑101 (MQ=255)
                                    gCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGcccc                                                             <  1:4546781/101‑1 (MQ=255)
                                    gCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGcccc                                                             <  2:1919381/101‑1 (MQ=255)
                                    gCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGcccc                                                             >  1:783537/1‑101 (MQ=255)
                                      tttGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCctc                                                           <  2:5596965/101‑1 (MQ=255)
                                       ttGACGGCCGCCGCCTCGGCTAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCctct                                                          >  1:3052679/1‑101 (MQ=255)
                                       ttGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCctct                                                          >  1:2990442/1‑101 (MQ=255)
                                        tGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCtt                                                         >  1:4335508/1‑101 (MQ=255)
                                         gACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTg                                                        <  2:4619978/101‑1 (MQ=255)
                                         gACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTg                                                        >  2:2227456/1‑101 (MQ=255)
                                            ggCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGATCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGccc                                                     <  1:4920560/101‑1 (MQ=255)
                                            ggCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGccc                                                     <  2:3394149/101‑1 (MQ=255)
                                            ggCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGccc                                                     <  1:5592374/101‑1 (MQ=255)
                                            ggCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGccc                                                     >  2:523810/1‑101 (MQ=255)
                                            ggCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGccc                                                     <  1:2804789/101‑1 (MQ=255)
                                             gccgccgccTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCccg                                                    >  1:5416697/1‑101 (MQ=255)
                                              ccgccgccTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCccgc                                                   >  2:4224014/1‑101 (MQ=255)
                                              ccgccgccGCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCccgc                                                   <  2:1063794/101‑1 (MQ=255)
                                                gccgccTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCccgccg                                                 >  2:1399233/1‑101 (MQ=255)
                                                gccgccTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCccgccg                                                 <  2:544021/101‑1 (MQ=255)
                                                gccgccTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCccgccg                                                 >  1:3095712/1‑101 (MQ=255)
                                                cccgccGCGGCGGGGCAGCGGGGGC.AAGCCCT.GGCCTTCGCGCTGGCCGCGCAAGGAGCCCGGGCAGGGGGGC.CCGCGCAGCGCCCCCCTTTTGCccgccg                                                 <  2:4153850/100‑1 (MQ=255)
                                                 cgcccTGGGGGAGGCGGCGGGGGC.AAGACCT.GCCTTTCGCGCGGGCCGCGCAAGGGGCCCGGGCAGGGGGGG.CCGCGCGGGGCGCCCCTCTTGCCCGCCGa                                                <  2:3089914/98‑1 (MQ=255)
                                                 ccgccTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGa                                                >  1:1019443/1‑101 (MQ=255)
                                                   gccTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATg                                              >  2:741618/1‑101 (MQ=255)
                                                     cgcgggcgggCGGCCGGGGC.ACGCGCT.GCCCTGCGCGGGGGCCCGGCAGTGGGCCGGGGCAAGGGGGG.CCGCCGGGCGCGCCCCTCTCCCCCGCCGATGCg                                            <  2:2826601/93‑1 (MQ=255)
                                                         gCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGc                                        >  2:91523/1‑101 (MQ=255)
                                                         gCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGc                                        >  1:4498266/1‑101 (MQ=255)
                                                           gAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCg                                      >  2:3682584/1‑101 (MQ=255)
                                                           gAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCg                                      >  2:4358969/1‑101 (MQ=255)
                                                            ggggcggcGGGGCAAAGCCCG.GCCCTCCGCGCGGGCGGCGAAAGGAG.CCGGGGAAGGGGGGCCCGCGCGGCCCCCCCTTTTTGCCCGCCGATGCGACGCCg                                      <  2:3355039/94‑1 (MQ=255)
                                                              gCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCgggg                                   >  1:3628709/1‑101 (MQ=255)
                                                                aGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCGGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGcc                                 >  1:3957157/1‑101 (MQ=255)
                                                                 gCCGGGGC.AAGCCCT.GGCCTGCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGccc                                <  2:1257938/101‑1 (MQ=255)
                                                                  ccGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCg                               >  1:2399260/1‑101 (MQ=255)
                                                                    ggggC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGa                             >  1:5242315/1‑101 (MQ=255)
                                                                    ggggA.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGa                             >  2:4900586/1‑101 (MQ=255)
                                                                     gggG.AAGGCCTGGGCCTCCGCGCGGGCCCCGAAAGGAGCGGGGGCAGGGGGGC.CCCCGCGGCGCCCCCCTTTTCCCCGCCGATGCGACGCCGGGGCCCGGa                             <  2:538469/101‑1 (MQ=255)
                                                                       gC.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAgcg                          >  2:2360970/1‑101 (MQ=255)
                                                                        c.AAGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAgcgc                         >  1:1698411/1‑101 (MQ=255)
                                                                          aaGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAGcgcg                        >  2:1819314/1‑101 (MQ=255)
                                                                           aGACCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAGCGCgg                       >  1:2642170/1‑101 (MQ=255)
                                                                             cccT.GGCCCTGGCGCGGGCCGCGAAAAGAGACGGGGGGGGGGGGG.CCCCCCGGCGCCCCCCTTTTCCCCGCCGATGCGACGCCGGGGCCCGGAGCGCGGaa                     <  1:1819424/100‑1 (MQ=255)
                                                                             aCCT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAGCGCGGaa                     >  2:535711/1‑101 (MQ=255)
                                                                              ccT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAGCGCGGAAc                    >  1:4820003/1‑101 (MQ=255)
                                                                               cT.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAGCGCGGAACt                   >  2:1747532/1‑101 (MQ=255)
                                                                                t.GGCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAGCGCGGAACtt                  >  1:1194977/1‑101 (MQ=255)
                                                                                 tgcccgccgcgCGGGCCGCGCAGGGAGCCGGGGCAGGGGGGC.CCGCCCGGCCCCCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAGCGCGGAACtt                  <  2:3178354/94‑1 (MQ=255)
                                                                                  ggCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAGCGCGGAACttt                 >  1:2701769/1‑101 (MQ=255)
                                                                                  ggCCTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAGCGCGGAACttt                 >  1:512722/1‑101 (MQ=255)
                                                                                  ggCCTCCGCGCGGCCCGCGCAAGGGGCCGGGGCGGGGGGGC.CCGCGCGGCCCCCCCCTCTCCCCCGCCGATGCGACGCCGGGGCCCGGAGCGCGGAACttt                 <  2:3045080/101‑1 (MQ=255)
                                                                                     cTTCGCGCTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAGCGCGGAACTTTCgg              >  1:2555789/1‑101 (MQ=255)
                                                                                         gcgcTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAGCGCGGAACTTTCGGGCCa          >  1:4620390/1‑101 (MQ=255)
                                                                                          cgcTGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAGCGCGGAACTTTCCGGCCAt         >  1:937394/1‑101 (MQ=255)
                                                                                             tGGCCGCGCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGGCGCTCTTGCCCGCCGATGGGAGGCCGGGGCCCGGAGCGCGGGAGGTTCGGGCCATCgg      >  2:5211018/1‑101 (MQ=255)
                                                                                             tGGCCGCGCAAGGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAGCGCGGAACTTTCGGGCCATCgg      <  2:2522410/101‑1 (MQ=255)
                                                                                                 cgcgCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAGCGCGGAACTTTCGGGCCATCGGGCCg  >  1:3457490/1‑101 (MQ=255)
                                                                                                 cgcgCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAGCGCGGAACTTTCGGGCCATCGGGCCg  >  2:2603455/1‑101 (MQ=255)
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ACGGACGAGGAACGGATGATCGCGATCGACACCGCGGCTTTGACGGCCGCCGCCTCGGCGAGGCAGCCGGGGC.AAGACCT.GGCCTTCGCGCTGGCCGCCCAATGAGACCGGGCAAGGGGGG.CCGCGCAGCGCGCCCCTCTTGCCCGCCGATGCGACGCCGGGGCCCGGAGCGCGGAACTTTCGGGCCATCGGGCCG  >  NC_014034/2389444‑2389639

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG