BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,407,597	A→C	100%	W83G (TGG→GGG)	nifH2 ←	nitrogenase iron protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,407,597	0	A→C	100.0%	869.4	228	W83G (TGG→GGG)	nifH2	nitrogenase iron protein
Reads supporting (aligned to +/- strand): new base (109/119): ref base (0/0): total (109/119)

Only 100 of 244 total aligned reads displayed.


GCGCAGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCAGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGATG  >  NC_014034/2407499‑2407694


gcgcAGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGa                                                                                                 >  2:5261038/1‑101 (MQ=255)


 cgcAGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGaa                                                                                                >  1:4893054/1‑101 (MQ=255)


   cAGCTCTTCGAGCAGATCGACCGCGGCCGAGATACCGCGCCCTGCGCAGCCCACCCCCGGCACCGTCCCTCCCCCCTCCCTCCCCCGAACGCCCCCTAAAc                                                                                              >  2:3049911/1‑101 (MQ=255)


    cgCTCTGCGAGCAGATCGCCCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAAcc                                                                                             <  1:5069001/100‑1 (MQ=255)


    aGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAAcc                                                                                             >  2:5650058/1‑101 (MQ=255)


     gCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCg                                                                                            >  2:3837388/1‑101 (MQ=255)


     gCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCg                                                                                            >  1:1859261/1‑101 (MQ=255)


     ccttttCGAGCAGACCGCCCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCCCCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCg                                                                                            <  2:5721380/97‑1 (MQ=255)


      ctctTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGa                                                                                           >  2:68864/1‑101 (MQ=255)


        ctTCGAGCAGATCGCCCGCGGCCGAGATGCCGCCCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcg                                                                                         <  1:3316641/101‑1 (MQ=255)


        ctTCGAGCAGATCGACCNCGGCGGAAATGCCGCCCCCGGCGCAGCCCACCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcg                                                                                         <  2:850871/101‑1 (MQ=255)


        ctTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcg                                                                                         >  2:1687054/1‑101 (MQ=255)


        ctTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcg                                                                                         >  1:1785863/1‑101 (MQ=255)


         tccGACCAGTTCGCCCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCCCCCCCGCCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcgg                                                                                        <  1:5346788/99‑1 (MQ=255)


           cGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcggcg                                                                                      >  2:3673801/1‑101 (MQ=255)


              gCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCTCGAAACCGACGGCGGaa                                                                                   >  1:409510/1‑101 (MQ=255)


               cAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGaaa                                                                                  >  1:252346/1‑101 (MQ=255)


               cAGACCCACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCCCCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGaaa                                                                                  <  1:578086/101‑1 (MQ=255)


                    cgcccGCGGCCGAGATGCCGCGCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCt                                                                             <  2:1620757/98‑1 (MQ=255)


                    cGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCt                                                                             >  1:1172974/1‑101 (MQ=255)


                    cGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCt                                                                             >  1:3554656/1‑101 (MQ=255)


                     gcccGCGGCCGCGCCCCCCCGCCGGGCGCAGCCCACCCCCGGCCCCGCCCCCCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCtt                                                                            <  2:5546233/99‑1 (MQ=255)


                     gACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCtt                                                                            >  2:1456280/1‑101 (MQ=255)


                      cccGCGGCCGAGTTGCCCCGCCCGGCCCACCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTc                                                                           <  1:882745/100‑1 (MQ=255)


                      cccGCGGCCGAGTGCCCGCCCCGGGCCCCCCCCCCCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTc                                                                           <  2:502940/100‑1 (MQ=255)


                       ccGCGCCGGAGAGGCCGCCCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTcc                                                                          <  2:1771220/101‑1 (MQ=255)


                        cgcgGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCa                                                                         >  2:5152360/1‑101 (MQ=255)


                        cgcgGCCGAGATGCCGCCCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCa                                                                         <  2:3401038/101‑1 (MQ=255)


                        cgcgCCCGAGATGCCGCCCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCa                                                                         <  1:2862179/101‑1 (MQ=255)


                         gcgGCCGAGATGCCGCGCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAg                                                                        <  1:2694833/101‑1 (MQ=255)


                          cgGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGc                                                                       >  1:3376293/1‑101 (MQ=255)


                          cgGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACCGCGGAAATGTCTTCCAGc                                                                       >  2:2373787/1‑101 (MQ=255)


                           ggCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCt                                                                      >  2:2824730/1‑101 (MQ=255)


                           ggCCGAGATGCCCCCCCCGGCCCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCt                                                                      <  1:4835227/101‑1 (MQ=255)


                            gCCGAGTTGCCGCGCCCGGCGCACCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCtt                                                                     <  2:3136174/101‑1 (MQ=255)


                            cccGAGACCCCGCCCCCGGCGCACCCCCCCCCGGCCCCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCtt                                                                     <  2:1859151/100‑1 (MQ=255)


                             ccGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTc                                                                    >  1:2186467/1‑101 (MQ=255)


                             ccGAGATCCCGCCCCCGGCGCACCCCCCCCCCGGCCCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTc                                                                    <  2:2276732/101‑1 (MQ=255)


                              ggagaCGCCGCCCCCGGGGCACCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCg                                                                   <  1:397511/100‑1 (MQ=255)


                              cgggaTCCCGCGCCCGGGGCCGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCg                                                                   <  2:486416/98‑1 (MQ=255)


                              cGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCg                                                                   >  2:1306814/1‑101 (MQ=255)


                               gagaTCCCGCCCCCGGCCCCCCCCCCCCCCGGCCCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCgg                                                                  <  1:1490677/101‑1 (MQ=255)


                                agaccccGCGCCCGGCCCAGCCCCCCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGt                                                                 <  2:3239149/96‑1 (MQ=255)


                                  aTGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGttt                                                               >  1:204371/1‑101 (MQ=255)


                                  aTGCCGCGCCCGGCGCACCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGttt                                                               <  2:3968209/101‑1 (MQ=255)


                                   tGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTg                                                              >  2:4091925/1‑101 (MQ=255)


                                    gCCGCGCCCGGCGCCGCCCCCCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGc                                                             <  2:2469397/101‑1 (MQ=255)


                                    cccGCCCCCGGCCCAGCCCCCCCCCGCCCCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGc                                                             <  2:2029199/100‑1 (MQ=255)


                                         gcCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCa                                                        >  1:1531068/1‑101 (MQ=255)


                                           cccgccccccccCCCCCCGGCCCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACg                                                      <  2:1510327/92‑1 (MQ=255)


                                           ccGGCGCAGCCCCCCCCCGGCACCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACg                                                      <  2:1969998/101‑1 (MQ=255)


                                           ccGGCCCACCCCCCCCCCGGCACCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACg                                                      <  2:5305196/101‑1 (MQ=255)


                                              gcgcGGCCCCCCCCGGGCCCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTc                                                   <  1:761061/101‑1 (MQ=255)


                                               ccccccccCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTcg                                                  <  1:4587602/96‑1 (MQ=255)


                                               ccccccccCCCCCCGGCCCCGCCCCCCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTcg                                                  <  1:5488985/96‑1 (MQ=255)


                                               ccccccccCCCCCCGGCCCCGCCCCCCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTcg                                                  <  1:1687153/96‑1 (MQ=255)


                                                  cccccCCCCCCGCCCCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCgcgc                                               <  1:746745/99‑1 (MQ=255)


                                                  aGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCgcgc                                               >  1:4604265/1‑101 (MQ=255)


                                                   gCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCa                                              <  2:551810/101‑1 (MQ=255)


                                                       ccccccGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCt                                          <  1:3033667/100‑1 (MQ=255)


                                                       aCCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCt                                          >  2:5796319/1‑101 (MQ=255)


                                                            cGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTATTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCa                                     >  2:111880/1‑101 (MQ=255)


                                                             gccccccccccccccgCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAg                                    <  2:1172893/93‑1 (MQ=255)


                                                              cccccGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGc                                   <  1:3202606/98‑1 (MQ=255)


                                                               cACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCa                                  >  1:1146438/1‑101 (MQ=255)


                                                                cccGGCCCCCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCAc                                 <  1:2667758/100‑1 (MQ=255)


                                                                  cGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACgg                               >  2:4811361/1‑101 (MQ=255)


                                                                    gcccgcccGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTg                             >  1:4310028/1‑101 (MQ=255)


                                                                    gcccgcccGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTg                             >  1:2759019/1‑101 (MQ=255)


                                                                     cccccccgCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTgg                            <  2:4400306/101‑1 (MQ=255)


                                                                      ccgcccgcCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTggg                           >  2:3653200/1‑101 (MQ=255)


                                                                       cccccgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGc                          <  1:4458220/99‑1 (MQ=255)


                                                                        gcccgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCa                         <  1:105686/101‑1 (MQ=255)


                                                                         cccgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAg                        >  1:1951638/1‑101 (MQ=255)


                                                                         cccgccTCGATGCACAGAACGCCCCCAAAACCGACGGCGGAAAAGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAg                        <  2:4777702/101‑1 (MQ=255)


                                                                            gccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTc                     >  2:910158/1‑101 (MQ=255)


                                                                            gccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTc                     <  1:2303260/101‑1 (MQ=255)


                                                                            gccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTc                     <  2:2758858/101‑1 (MQ=255)


                                                                             ccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCt                    <  1:3837630/101‑1 (MQ=255)


                                                                             ccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCt                    <  2:2581894/101‑1 (MQ=255)


                                                                             ccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGCCCAGCACGTTGGCCAGTTCt                    <  2:4591265/101‑1 (MQ=255)


                                                                              gtCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTg                   >  2:375824/2‑101 (MQ=255)


                                                                              cTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTg                   >  1:1415832/1‑101 (MQ=255)


                                                                                cGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGcc                 <  2:3567462/101‑1 (MQ=255)


                                                                                  aTGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGcccc               >  2:237427/1‑101 (MQ=255)


                                                                                   tGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCTAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTTTCCAGCACTGTGGGCAGTTCTCCCccg              <  1:5734281/101‑1 (MQ=255)


                                                                                    gCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCccgc             <  2:2700288/101‑1 (MQ=255)


                                                                                     cacaGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCccgcc            >  1:3088117/1‑101 (MQ=255)


                                                                                      acaGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCccgccg           <  2:4190327/101‑1 (MQ=255)


                                                                                       caGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCCCGGTGGGCAGTTCTGCCCcgccgc          <  2:3684770/101‑1 (MQ=255)


                                                                                        aGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCcgcg         <  2:1530975/101‑1 (MQ=255)


                                                                                        aGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCcgcg         <  2:358639/101‑1 (MQ=255)


                                                                                        aGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCcgcg         <  2:635575/101‑1 (MQ=255)


                                                                                        aGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCcgcg         >  2:3866456/1‑101 (MQ=255)


                                                                                        aGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTCCCCCGCcgcg         <  1:17114/101‑1 (MQ=255)


                                                                                             gacccCGAACCCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCAGGGTCGCGCAGGCTGTCCGGGGCGGTGGGCTGTTCTAAGCGGCCACGCAGGa    <  2:2416087/99‑1 (MQ=255)


                                                                                             gCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGa    <  1:4757898/101‑1 (MQ=255)


                                                                                             gCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGAAGTTCTGCCCCGCCGCGCAGGa    >  2:301680/1‑101 (MQ=255)


                                                                                               cccCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGAtg  <  1:3529123/101‑1 (MQ=255)


                                                                                               cccCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGAtg  <  1:1577201/101‑1 (MQ=255)


GCGCAGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCAGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGATG  >  NC_014034/2407499‑2407694

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG