Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,018 G→C 7.7% intergenic (+66/‑176) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0180G→C 7.7% 25.1 ‑1.8 0.0178intergenic (+66/‑176)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/5):  ref base (68/96):  total (77/101)
Fisher's exact test strand distribution p-value = 1.58e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 190 total aligned reads displayed.

CCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AA..GGGGGGG.CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGG  >  NC_014034/2493919‑2494117
                                                                                                       |                                                                                                         
  gcggcgGGCGCGCGGCACGCCAGGCCGAGTCAG..GGGGGGG.CGCGGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.At                                                                                                        <  1:369795/101‑1 (MQ=18)
  gcggcgGGCGCGCGGCACGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.At                                                                                                        <  2:5533574/101‑1 (MQ=21)
       gggcgGGCGGCACGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTGCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCtt                                                                                                   <  2:1908503/101‑1 (MQ=18)
       gggcgCGCGGCACGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCtt                                                                                                   <  2:4272241/101‑1 (MQ=21)
       gggcgCGCGGCACGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACCCCCCCCCGGACATTTGCAGCCAGATGAAAACGG.AGGccgt                                                                                                   >  1:5716720/1‑99 (MQ=18)
        ggcgTGCGGCACGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGGTATTTGCAGCAAGATGAAACCGG.ATGCCttt                                                                                                  >  1:5239086/1‑101 (MQ=18)
        ggcgCGCGGCACGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCttt                                                                                                  <  1:404052/101‑1 (MQ=21)
         gcgcgcGGCACGACAGGCCGAGT.AAGGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCttt                                                                                                  <  2:4441272/101‑1 (MQ=18)
         gcgcgcGGCACGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTc                                                                                                 <  1:3257067/101‑1 (MQ=21)
          cgcgcgGCACGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCa                                                                                                >  1:1526278/1‑101 (MQ=21)
           ccgcgGCACGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCAt                                                                                               <  2:3005454/100‑1 (MQ=18)
            cgcgGCACGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATc                                                                                              <  2:2335079/101‑1 (MQ=21)
             gcgGCACGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCt                                                                                             <  2:3722868/101‑1 (MQ=21)
              cgGCACGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCtt                                                                                            <  2:3465855/101‑1 (MQ=21)
              cgGCACGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCtt                                                                                            <  1:4739050/101‑1 (MQ=21)
               ggCACGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTg                                                                                           <  1:4043740/101‑1 (MQ=21)
                 cgcGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGcc                                                                                         <  1:334478/99‑1 (MQ=18)
                 cACGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGcc                                                                                         >  2:5366437/1‑101 (MQ=21)
                  aCGACAGGCCGAGT.AAG.GGGGGGG.CTCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGccc                                                                                        <  2:2269814/101‑1 (MQ=18)
                  aCGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGccc                                                                                        <  1:513639/101‑1 (MQ=21)
                  aCGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGccc                                                                                        <  1:2862297/101‑1 (MQ=21)
                  aCGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGccc                                                                                        >  1:2371653/1‑101 (MQ=21)
                  aCGACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGGCCGCCAGCGGACTCCCCCCCGGATATTTGCCGCAAGAAGAAAACGGAAGGCCTTTCTTCtgccc                                                                                         >  1:1041465/1‑97 (MQ=9)
                    gACAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCCG.ATGCCTTTCATCTTGgccca                                                                                      >  1:2620391/1‑99 (MQ=18)
                      cAGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGaaa                                                                                    >  2:1955983/1‑101 (MQ=21)
                       aGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAat                                                                                   <  2:4539795/101‑1 (MQ=21)
                       aGGCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAat                                                                                   <  2:3911735/101‑1 (MQ=21)
                       aGGCCGAGT.AA..GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAata                                                                                  <  1:4922381/101‑1 (MQ=255)
                         gCCGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAatat                                                                                 <  1:3596599/101‑1 (MQ=21)
                         gCCGAGT.AA..GGGGGGGCCGCTGCCCCCCGTCCGCAAGCGGCCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAatat                                                                                 <  2:5644917/101‑1 (MQ=21)
                          ccGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATc                                                                                <  2:1002550/101‑1 (MQ=18)
                          ccGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATc                                                                                >  2:5270971/1‑101 (MQ=18)
                          ccGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATc                                                                                <  2:5480580/101‑1 (MQ=21)
                           cGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATcc                                                                               >  1:1610409/1‑101 (MQ=18)
                           cGAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATcc                                                                               <  1:1768512/101‑1 (MQ=21)
                            gAGT.AAG.GGGGGGG.CGGTGCCCCCCGTCCGCAAGCGGTCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATccc                                                                              <  2:4730446/101‑1 (MQ=18)
                            gAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATccc                                                                              >  1:1109696/1‑101 (MQ=18)
                            gAGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATccc                                                                              >  2:5357442/1‑101 (MQ=18)
                             aGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCg                                                                             >  1:1711471/1‑101 (MQ=18)
                             aGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCg                                                                             <  2:410552/101‑1 (MQ=18)
                             aGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCg                                                                             <  1:1851003/101‑1 (MQ=18)
                             aGT.AAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCg                                                                             <  2:1405575/101‑1 (MQ=18)
                               ggaaggggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCg                                                                             <  2:3377528/95‑1 (MQ=18)
                                gtaagggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                            >  2:5087086/6‑101 (MQ=255)
                                 taagggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                           >  1:4504453/5‑101 (MQ=255)
                                   agggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                         <  1:2298560/99‑1 (MQ=255)
                                   agggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                         >  2:3237842/3‑101 (MQ=255)
                                   agggggggg.CGCTGCCCCCCGTCCGCAAGCGGACCCCCCCCGGGGTTTTTGCCGCCAGCTGACACCGG.ATGCCCTTCATCTTCCCCTAAATAATCCggggg                                                                         >  2:2288534/3‑101 (MQ=12)
                                    gggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                        <  1:4207758/100‑1 (MQ=255)
                                      gggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                      <  1:5728365/101‑1 (MQ=255)
                                       ggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                     <  1:3411368/101‑1 (MQ=255)
                                        gggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                    >  2:2477211/1‑101 (MQ=255)
                                         ggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                  <  1:679154/101‑1 (MQ=255)
                                         ggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                  <  1:1688307/101‑1 (MQ=255)
                                             cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                               >  2:3060121/1‑101 (MQ=255)
                                               cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                          <  1:536070/101‑1 (MQ=255)
                                                tGCCCCCCGTCCGCAAGCGGGCTCCCCCCGGGATATTTGCAGCAAGATGCAACCGG.GTGCCTTTCCTCTTTTCCCAAATAACCCGGGGGGGGacaggggaa                                                             >  2:4961732/1‑93 (MQ=12)
                                                tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                         <  2:3987542/101‑2 (MQ=255)
                                                tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aa                                                         >  1:5283323/1‑100 (MQ=255)
                                                 gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                        <  2:4908975/101‑3 (MQ=255)
                                                 gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                        <  1:4336343/101‑3 (MQ=255)
                                                 gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aag                                                        >  1:2070495/1‑99 (MQ=255)
                                                  cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                      <  2:3399449/101‑5 (MQ=255)
                                                  cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                      <  1:1249727/101‑5 (MQ=255)
                                                  cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..G.aagcc                                                      >  2:1913058/1‑97 (MQ=18)
                                                   ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ACGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGCC..G.aagaca                                                     >  2:883429/1‑96 (MQ=18)
                                                      ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                   >  2:628593/4‑94 (MQ=255)
                                                      ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACAGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccaga                                                   >  2:918705/4‑94 (MQ=18)
                                                       cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                  <  1:900261/99‑9 (MQ=255)
                                                       cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                  <  1:2977745/99‑9 (MQ=255)
                                                        gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                 <  2:431362/100‑10 (MQ=255)
                                                        gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                 <  2:1256930/100‑10 (MQ=255)
                                                                gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagc                                          >  1:101779/1‑99 (MQ=18)
                                                                     cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccc                                   >  1:2722749/1‑101 (MQ=18)
                                                                      tCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.ccccc                                  >  2:593976/1‑101 (MQ=18)
                                                                      tCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.ccccc                                  >  2:224865/1‑101 (MQ=17)
                                                                       ccccccGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccc                                 >  2:1216098/1‑100 (MQ=18)
                                                                         ccccGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccctg                               <  1:1153654/101‑4 (MQ=17)
                                                                          cccGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccctgc                              <  2:1085111/101‑5 (MQ=17)
                                                                            cGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGccc                             <  1:2517749/101‑1 (MQ=17)
                                                                             gggATATTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGcccc                            >  2:5233772/1‑101 (MQ=0)
                                                                              ggATATTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcg                           <  1:1558387/101‑1 (MQ=0)
                                                                              ggATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcg                           <  2:5706753/101‑1 (MQ=17)
                                                                               gATATTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgc                          >  1:2232856/1‑100 (MQ=2)
                                                                               gATATTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgc                          >  2:1503599/1‑100 (MQ=2)
                                                                               gATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcgg                          >  1:5353248/1‑101 (MQ=18)
                                                                                atatTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcggc                         >  2:1738181/1‑101 (MQ=17)
                                                                                atatTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcggc                         >  2:2837301/1‑101 (MQ=17)
                                                                                atatTGGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgcc                         <  1:5657283/101‑3 (MQ=2)
                                                                                 tatTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgccg                        >  2:254360/1‑101 (MQ=2)
                                                                                     tGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCGGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                    <  2:3938891/101‑1 (MQ=18)
                                                                                         gcaAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTgg                >  2:329376/1‑101 (MQ=18)
                                                                                          caAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggc               >  1:3195975/1‑101 (MQ=18)
                                                                                          caAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggc               >  2:1579395/1‑101 (MQ=17)
                                                                                            aGATGAAACCGG.ATTCCGTTCATCTTGCCTGAAATATTCCGGGGGGTGAA.TGGA..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg             <  1:2846712/101‑1 (MQ=9)
                                                                                            aGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg             >  2:4204943/1‑101 (MQ=18)
                                                                                                 aaaCCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCt        >  1:5411536/1‑101 (MQ=18)
                                                                                                  acccGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGGCCtt       <  1:1954441/99‑1 (MQ=17)
                                                                                                  aaCCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt       >  1:3949650/1‑101 (MQ=18)
                                                                                                       g.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg  >  2:342131/1‑101 (MQ=18)
                                                                                                       |                                                                                                         
CCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AA..GGGGGGG.CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGG  >  NC_014034/2493919‑2494117

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG