Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,023 C→A 7.9% intergenic (+71/‑171) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0230C→A 7.9% 26.6 ‑1.8 ‑0.1177intergenic (+71/‑171)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/5):  ref base (72/91):  total (81/96)
Fisher's exact test strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.92e-01

Only 100 of 193 total aligned reads displayed.

GCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGG.CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CC.C.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGG  >  NC_014034/2493924‑2494122
                                                                                                      |                                                                                                          
 cgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCt                                                                                                           <  1:5606574/101‑1 (MQ=21)
 cgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCt                                                                                                           >  1:3114725/1‑101 (MQ=21)
  gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCtt                                                                                                          <  2:4272241/101‑1 (MQ=21)
  gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCtt                                                                                                          <  1:3340066/101‑1 (MQ=21)
  gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCtt                                                                                                          <  1:195317/101‑1 (MQ=21)
   gggggggggCCCGCCAGGCCGAGTAAGGGGGGGG.CGCTCCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCttt                                                                                                         <  1:3210721/94‑1 (MQ=17)
   ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCttt                                                                                                         <  1:1091910/101‑1 (MQ=21)
   ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCttt                                                                                                         <  1:404052/101‑1 (MQ=21)
     cgcgcgGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCa                                                                                                       >  1:1526278/1‑101 (MQ=21)
      gcgcgGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCAt                                                                                                      <  1:5128591/101‑1 (MQ=21)
      gcgcgGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCAt                                                                                                      <  2:5376790/101‑1 (MQ=21)
       cgcgGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATc                                                                                                     <  2:2335079/101‑1 (MQ=21)
        gcgGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCt                                                                                                    <  2:3431272/101‑1 (MQ=21)
             aCGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGccc                                                                                               <  2:4743496/101‑1 (MQ=21)
             aCGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGGCCGCCAGCGGACTCCCCCCCGGATATTTGCCGCAAGAAGAAAACGGAAGGCCTTTCTTCtgccc                                                                                                >  1:1041465/1‑97 (MQ=9)
                aCAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGaa                                                                                            <  2:5258250/101‑1 (MQ=21)
                 cAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGaaa                                                                                           <  1:2331066/101‑1 (MQ=21)
                 cAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGaaa                                                                                           >  2:1955983/1‑101 (MQ=21)
                  aGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAat                                                                                          <  2:3911735/101‑1 (MQ=21)
                  aGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAat                                                                                          >  2:3411163/1‑101 (MQ=21)
                  aGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAat                                                                                          <  2:4629544/101‑1 (MQ=21)
                   ggCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAata                                                                                         >  1:4318997/1‑101 (MQ=21)
                    gccgggtaggggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAatat                                                                                        <  1:290385/91‑1 (MQ=18)
                    gCCGAGTAAGGGGGGGG.CGTTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAatat                                                                                        <  2:438697/101‑1 (MQ=18)
                    gCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAatat                                                                                        <  1:3597734/101‑1 (MQ=21)
                    gCCGAGTAA.GGGGGGGCCGCTGCCCCCCGTCCGCAAGCGGCCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAatat                                                                                        <  2:5644917/101‑1 (MQ=21)
                     ccGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATc                                                                                       <  2:329755/101‑1 (MQ=21)
                      cGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATcc                                                                                      <  1:1768512/101‑1 (MQ=21)
                      cGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATcc                                                                                      >  1:1610409/1‑101 (MQ=18)
                       gAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATccc                                                                                     >  2:5357442/1‑101 (MQ=18)
                       gAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATccc                                                                                     >  1:5668494/1‑101 (MQ=18)
                       gAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATccc                                                                                     >  1:1109696/1‑101 (MQ=18)
                        aGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCCAGCGGACTCCCACCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCg                                                                                    <  2:2268504/101‑1 (MQ=18)
                        aGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCg                                                                                    >  1:1711471/1‑101 (MQ=18)
                        aGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCg                                                                                    <  2:410552/101‑1 (MQ=18)
                         gtaagggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                   <  2:4477155/96‑1 (MQ=255)
                         gtaagggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                   >  2:5087086/6‑101 (MQ=255)
                         gtaagggggggg.CGCGGCCCACCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                   <  2:1457348/96‑1 (MQ=18)
                           aagggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                                 >  2:190980/4‑101 (MQ=255)
                            agggggggg.CGCTGCCCCCCGTCCGCAAGCGGACCCCCCCCGGGGTTTTTGCCGCCAGCTGACACCGG.ATGCCCTTCATCTTCCCCTAAATAATCCggggg                                                                                >  2:2288534/3‑101 (MQ=12)
                              ggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                              <  2:4936145/101‑1 (MQ=255)
                                 gggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                           <  1:4076109/101‑1 (MQ=255)
                                 gggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                           >  2:2477211/1‑101 (MQ=255)
                                  ggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                         <  1:1688307/101‑1 (MQ=255)
                                    g.cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.Tgg                                                                       >  1:1959831/1‑101 (MQ=255)
                                       gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                                   >  2:3684816/1‑101 (MQ=255)
                                        cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTTCCGCAAGATGAAACCGG.ATGCCTTTCATCTGGCCCGAAATATCCCGGGGGGTGAA.TGGcaa                                                                    >  2:1679067/1‑99 (MQ=21)
                                         tGCCCCCCGTCCGCAAGCGGGCTCCCCCCGGGATATTTGCAGCAAGATGCAACCGG.GTGCCTTTCCTCTTTTCCCAAATAACCCGGGGGGGGacaggggaa                                                                    >  2:4961732/1‑93 (MQ=12)
                                         tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                                <  2:3987542/101‑2 (MQ=255)
                                          gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGGCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aac                                                               >  1:5799507/1‑99 (MQ=18)
                                           cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                             <  2:3399449/101‑5 (MQ=255)
                                           cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..G.aagcc                                                             >  2:1913058/1‑97 (MQ=18)
                                            ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                            <  1:4722280/101‑6 (MQ=255)
                                            ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ACGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGCC..G.aagaca                                                            >  2:883429/1‑96 (MQ=18)
                                              cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                           <  1:4838278/97‑7 (MQ=255)
                                               ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                          >  2:628593/4‑94 (MQ=255)
                                               ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACAGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccaga                                                          >  2:918705/4‑94 (MQ=18)
                                                  tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagaggg                                                       <  1:2566573/101‑11 (MQ=255)
                                                         gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagc                                                 >  1:101779/1‑99 (MQ=18)
                                                          cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcg                                                >  2:128492/1‑98 (MQ=18)
                                                          cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcg                                                >  2:2783474/1‑98 (MQ=18)
                                                           ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcgc                                               >  1:2080257/1‑97 (MQ=18)
                                                              cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cc.c.c                                        >  1:2722749/1‑101 (MQ=18)
                                                               tCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cc.c.cc                                       >  2:593976/1‑101 (MQ=18)
                                                                ccccccGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cc.c.ccc                                      >  2:1216098/1‑100 (MQ=18)
                                                                ccccccGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cc.c.ccc                                      >  2:2990638/1‑100 (MQ=17)
                                                                  ccccGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cc.c.ccctg                                    <  1:1153654/101‑4 (MQ=17)
                                                                     cGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGccc                                  <  1:3748748/101‑1 (MQ=17)
                                                                     cGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGccc                                  >  1:3401375/1‑101 (MQ=17)
                                                                      gggATATTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.C.CCTGcccc                                 >  2:5233772/1‑101 (MQ=0)
                                                                       ggATATTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.C.CCTGCCCcg                                <  1:1558387/101‑1 (MQ=0)
                                                                       ggATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGCCCcg                                <  2:5706753/101‑1 (MQ=17)
                                                                       ggATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGAGC.AGCGCCC.C.CCTGCCCcg                                >  1:3456871/1‑101 (MQ=17)
                                                                        gATATTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.C.CCTGCCCcgc                               >  1:2232856/1‑100 (MQ=2)
                                                                        gATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGCCCcgg                               >  1:5353248/1‑101 (MQ=18)
                                                                        gATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAACCAGAGGGGGGC.AGCGCCC.C.CCTGCCCcgg                               >  1:5262642/1‑101 (MQ=17)
                                                                         atatTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.C.CCTGCCCcgcc                              >  1:2461050/1‑99 (MQ=2)
                                                                         atatTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.C.CCTGCCCcgcc                              >  1:97697/1‑99 (MQ=2)
                                                                         atatTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.C.CCTGCCCcgcc                              <  2:3021862/101‑3 (MQ=2)
                                                                         atatTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.C.CCTGCCCcgcc                              >  2:1511896/1‑99 (MQ=2)
                                                                             ttGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.C.CCTGCCCcgccggat                          <  2:3238589/101‑3 (MQ=2)
                                                                              tGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCGGAGGGGGGC.AGCGCCC.C.CCTGCCCCGGCGGTcc                         <  2:3938891/101‑1 (MQ=18)
                                                                              tGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGCCCCGGCGGTcc                         <  2:5492465/101‑1 (MQ=17)
                                                                               gcagcaAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGCCCCGGGGGGCCg                        >  1:4383610/1‑101 (MQ=14)
                                                                                cagcaAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.CCAC.CCTGCCCCGGCGGTCCgg                       >  2:1786314/1‑100 (MQ=18)
                                                                                     aGATGAAACCGG.ATTCCGTTCATCTTGCCTGAAATATTCCGGGGGGTGAA.TGGA..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGCCCCGGCGGTCCGTggcgg                  <  1:2846712/101‑1 (MQ=9)
                                                                                     aGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGCCCCGGCGGTCCGTggcgg                  >  2:4204943/1‑101 (MQ=18)
                                                                                          aaaCCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGCCCCGGCGGTCCGTGGCGGCGCCt             >  1:5411536/1‑101 (MQ=18)
                                                                                          aaaCCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGCCCCGGCGGTCCGTGGCGGCGCCt             <  2:101759/101‑1 (MQ=17)
                                                                                           aaCCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGCCCCGGCGGTCCGTGGCGGCGCCtt            >  1:5728138/1‑101 (MQ=18)
                                                                                             ccGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGG..GAAGCCCGAGGGGGGC.AGCG.CC.CGCCTGCCCCGGCGGCCCGGCGCGGCGCCTGGa          >  1:5192999/1‑101 (MQ=9)
                                                                                              cGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc         >  1:745251/1‑101 (MQ=18)
                                                                                               gg.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg        >  2:3084474/1‑101 (MQ=17)
                                                                                                   tcccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc     <  2:1154967/99‑1 (MQ=17)
                                                                                                   tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCAGCAC.CC.C.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc     >  1:5365492/1‑101 (MQ=18)
                                                                                                   tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc     <  2:5283033/101‑1 (MQ=17)
                                                                                                   tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc     >  2:2526353/1‑101 (MQ=18)
                                                                                                      cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg  >  1:3962078/1‑101 (MQ=18)
                                                                                                      cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg  >  1:2635853/1‑101 (MQ=18)
                                                                                                      cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.C.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg  >  1:138099/1‑101 (MQ=17)
                                                                                                      |                                                                                                          
GCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGG.CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CC.C.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGG  >  NC_014034/2493924‑2494122

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG